fgf21 knockout fgf21ko mice (Taconic Biosciences)

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Taconic Biosciences fgf21 knockout fgf21ko mice

<t>FGF21</t> is primarily expressed in the liver and is dispensable for the physiological response to fasting . WT and <t>FGF21KO</t> animals were either allowed ad libidum access to food (fed) or subjected to a 24-h fast (fasted). (A) Plasma FGF21 levels were measured in WT animals. (B) FGF21 mRNA and (C) protein expression were measured in liver and adipose tissue from WT animals. (D) Blood glucose was assessed via glucometer in each cohort. Plasma was collected from WT and FGF21KO animals and used to determine circulating levels of (E) insulin, (F) βHB, (G) triglycerides, and (H) free fatty acids. The concentration of (I) triglycerides and (J) glycogen was measured in the livers of WT and FGF21KO animals. (K) A pyruvate tolerance test was performed on a separate cohort of WT and FGF21KO animals by administration of 2 g/kg sodium pyruvate following a 22-h fast. * p < 0.05 vs. fed; α, p < 0.05 vs. WT Fed; β, p < 0.05 vs. FGF21KO fed; φ, p < 0.05 vs. WT fasted; #, p < 0.05 vs. WT. No statistical difference was observed in fed measurements between genotypes.

Fgf21 Knockout Fgf21ko Mice, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fgf21/Fgf21/pmc04928592-37-3-7
Average 93 stars, based on 8 article reviews

fgf21 knockout fgf21ko mice - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "Fibroblast Growth Factor 21-Null Mice Do Not Exhibit an Impaired Response to Fasting"

Article Title: Fibroblast Growth Factor 21-Null Mice Do Not Exhibit an Impaired Response to Fasting

Journal: Frontiers in Endocrinology

doi: 10.3389/fendo.2016.00077

FGF21 is primarily expressed in the liver and is dispensable for the physiological response to fasting . WT and FGF21KO animals were either allowed ad libidum access to food (fed) or subjected to a 24-h fast (fasted). (A) Plasma FGF21 levels were measured in WT animals. (B) FGF21 mRNA and (C) protein expression were measured in liver and adipose tissue from WT animals. (D) Blood glucose was assessed via glucometer in each cohort. Plasma was collected from WT and FGF21KO animals and used to determine circulating levels of (E) insulin, (F) βHB, (G) triglycerides, and (H) free fatty acids. The concentration of (I) triglycerides and (J) glycogen was measured in the livers of WT and FGF21KO animals. (K) A pyruvate tolerance test was performed on a separate cohort of WT and FGF21KO animals by administration of 2 g/kg sodium pyruvate following a 22-h fast. * p < 0.05 vs. fed; α, p < 0.05 vs. WT Fed; β, p < 0.05 vs. FGF21KO fed; φ, p < 0.05 vs. WT fasted; #, p < 0.05 vs. WT. No statistical difference was observed in fed measurements between genotypes.

Figure Legend Snippet: FGF21 is primarily expressed in the liver and is dispensable for the physiological response to fasting . WT and FGF21KO animals were either allowed ad libidum access to food (fed) or subjected to a 24-h fast (fasted). (A) Plasma FGF21 levels were measured in WT animals. (B) FGF21 mRNA and (C) protein expression were measured in liver and adipose tissue from WT animals. (D) Blood glucose was assessed via glucometer in each cohort. Plasma was collected from WT and FGF21KO animals and used to determine circulating levels of (E) insulin, (F) βHB, (G) triglycerides, and (H) free fatty acids. The concentration of (I) triglycerides and (J) glycogen was measured in the livers of WT and FGF21KO animals. (K) A pyruvate tolerance test was performed on a separate cohort of WT and FGF21KO animals by administration of 2 g/kg sodium pyruvate following a 22-h fast. * p < 0.05 vs. fed; α, p < 0.05 vs. WT Fed; β, p < 0.05 vs. FGF21KO fed; φ, p < 0.05 vs. WT fasted; #, p < 0.05 vs. WT. No statistical difference was observed in fed measurements between genotypes.

Techniques Used: Clinical Proteomics, Expressing, Concentration Assay

Oxidative and gluconeogenic gene expression is attenuated in the liver, while lipase gene expression and activity in WAT remains unaltered in fasted FGF21KO mice . (A) In the liver, expressions of genes associated with fatty acid oxidation (CPT1a, ACADS, ACADM, ACADL, ACADVL, HADH), fatty acid import (CD36), gluconeogenesis (G6PC, PCK1), and ketone body production (HMGCS2, BDH1) were measured. (B) Expressions of genes associated with lipolysis (HSL, ATGL, LPL), fatty acid synthesis (FASN), fatty acid import (CD36), and thermogenesis (CIDEA, UCP1) were measured. In adipose tissue: α, p < 0.05 vs. WT Fed; β, p < 0.05 vs. FGF21KO fed; φ, p < 0.05 vs. WT fasted. No statistical differences were observed between genotypes in fed expression of any gene measured. (C) The amount of total and phosphorylated HSL was assessed by Western blot in WAT of WT and FGF21KO animals in both the fed and fasted state.

Figure Legend Snippet: Oxidative and gluconeogenic gene expression is attenuated in the liver, while lipase gene expression and activity in WAT remains unaltered in fasted FGF21KO mice . (A) In the liver, expressions of genes associated with fatty acid oxidation (CPT1a, ACADS, ACADM, ACADL, ACADVL, HADH), fatty acid import (CD36), gluconeogenesis (G6PC, PCK1), and ketone body production (HMGCS2, BDH1) were measured. (B) Expressions of genes associated with lipolysis (HSL, ATGL, LPL), fatty acid synthesis (FASN), fatty acid import (CD36), and thermogenesis (CIDEA, UCP1) were measured. In adipose tissue: α, p < 0.05 vs. WT Fed; β, p < 0.05 vs. FGF21KO fed; φ, p < 0.05 vs. WT fasted. No statistical differences were observed between genotypes in fed expression of any gene measured. (C) The amount of total and phosphorylated HSL was assessed by Western blot in WAT of WT and FGF21KO animals in both the fed and fasted state.

Techniques Used: Gene Expression, Activity Assay, Expressing, Western Blot

Endogenous FGF21 is not required for proper lipid and carbohydrate metabolism during the fed to fasted transition . At the beginning of the light cycle, WT animals had their food removed and were administered 5 mg/kg ΔN17 once every 3 h for 12 h. (A) Blood glucose measurements were taken via glucometer at the specified time points. Circulating levels of (B) insulin, (C) βHB, (D) triglycerides, and (E) free fatty acids were determined from plasma samples collected at the end of the study. Liver samples were taken at the end of the study and used to determine the concentrations of (F) triglycerides and (G) glycogen. (H) In the liver, expression of FGF21 as well as genes associated with fatty acid oxidation (CPT1a, ACADS, ACADM, ACADL, ACADVL, HADH), fatty acid import (CD36), gluconeogenesis (G6PC, PCK1), and ketone body production (HMGCS2, BDH1) were measured. (I) Expression of FGF21 in addition to genes associated with lipolysis (HSL, ATGL, LPL), fatty acid synthesis (FASN), fatty acid import (CD36), and thermogenesis (CIDEA, UCP1) were measured in adipose tissue. * p < 0.05 vs. PBS.

Figure Legend Snippet: Endogenous FGF21 is not required for proper lipid and carbohydrate metabolism during the fed to fasted transition . At the beginning of the light cycle, WT animals had their food removed and were administered 5 mg/kg ΔN17 once every 3 h for 12 h. (A) Blood glucose measurements were taken via glucometer at the specified time points. Circulating levels of (B) insulin, (C) βHB, (D) triglycerides, and (E) free fatty acids were determined from plasma samples collected at the end of the study. Liver samples were taken at the end of the study and used to determine the concentrations of (F) triglycerides and (G) glycogen. (H) In the liver, expression of FGF21 as well as genes associated with fatty acid oxidation (CPT1a, ACADS, ACADM, ACADL, ACADVL, HADH), fatty acid import (CD36), gluconeogenesis (G6PC, PCK1), and ketone body production (HMGCS2, BDH1) were measured. (I) Expression of FGF21 in addition to genes associated with lipolysis (HSL, ATGL, LPL), fatty acid synthesis (FASN), fatty acid import (CD36), and thermogenesis (CIDEA, UCP1) were measured in adipose tissue. * p < 0.05 vs. PBS.

Techniques Used: Clinical Proteomics, Expressing

Blockade of FGF21 signaling during late fasting does not impair the fasted response . WT mice were fasted for 21 h, administered 5 mg/kg ΔN17, and then subjected to a PTT 1 h later by administration of 2 g/kg sodium pyruvate. (A) Blood glucose was measured at the specified time points via glucometer. A separate cohort of WT mice was fasted for 23 h, administered 5 mg/kg ΔN17, and then sacrificed 1 h later. (B) Blood glucose was assessed via glucometer. Plasma samples were collected and used to determine circulating levels of (C) insulin, (D) βHB, (E) triglycerides, and (F) free fatty acids. Hepatic (G) Triglyceride and (H) glycogen content was measured. (I) In the liver, expression of FGF21 as well as genes associated with fatty acid oxidation (CPT1a, ACADS, ACADM, ACADL, ACADVL, HADH), fatty acid import (CD36), gluconeogenesis (G6PC, PCK1), and ketone body production (HMGCS2, BDH1) were measured. (J) Expression of FGF21 in addition to genes associated with lipolysis (HSL, ATGL, LPL), fatty acid synthesis (FASN), fatty acid import (CD36), and thermogenesis (CIDEA, UCP1) were measured in adipose tissue. * p < 0.05 vs. PBS.

Figure Legend Snippet: Blockade of FGF21 signaling during late fasting does not impair the fasted response . WT mice were fasted for 21 h, administered 5 mg/kg ΔN17, and then subjected to a PTT 1 h later by administration of 2 g/kg sodium pyruvate. (A) Blood glucose was measured at the specified time points via glucometer. A separate cohort of WT mice was fasted for 23 h, administered 5 mg/kg ΔN17, and then sacrificed 1 h later. (B) Blood glucose was assessed via glucometer. Plasma samples were collected and used to determine circulating levels of (C) insulin, (D) βHB, (E) triglycerides, and (F) free fatty acids. Hepatic (G) Triglyceride and (H) glycogen content was measured. (I) In the liver, expression of FGF21 as well as genes associated with fatty acid oxidation (CPT1a, ACADS, ACADM, ACADL, ACADVL, HADH), fatty acid import (CD36), gluconeogenesis (G6PC, PCK1), and ketone body production (HMGCS2, BDH1) were measured. (J) Expression of FGF21 in addition to genes associated with lipolysis (HSL, ATGL, LPL), fatty acid synthesis (FASN), fatty acid import (CD36), and thermogenesis (CIDEA, UCP1) were measured in adipose tissue. * p < 0.05 vs. PBS.

Techniques Used: Clinical Proteomics, Expressing

Knock-Out:

Article Title: FGF21 Is Increased by Inflammatory Stimuli and Protects Leptin-Deficient ob/ob Mice from the Toxicity of Sepsis
Article Snippet: For the FGF21 protection studies female ob/ob and C57BL/6 mice were purchased from Harlan UK (Belton Loughborough, UK). .. FGF21 knockout (KO) mice were purchased from Taconic Farms, Inc. (Hudson, NY). ..

Article Title: Anti-inflammatory effects of exercise training in adipose tissue do not require FGF21
Article Snippet: .. Male FGF21 knockout mice on a C57BL/6NTac background (FGF21KO) and age-matched, but not littermate, C57BL/6NTac WT mice controls were bred by Taconic Biosciences (Hudson, NY) and kindly provided by Eli Lilly (Indianapolis, IN). ..

Article Title: Fibroblast Growth Factor 21-Null Mice Do Not Exhibit an Impaired Response to Fasting
Article Snippet: Animal protocols in this study were approved by the Eli Lilly and Co., Animal Use and Care Committee (Protocol No. 13-030). .. Male C57Bl/6J and FGF21 knockout (FGF21KO) mice (Taconic Farms) were maintained on a standard chow diet consisting of 13% fat, 67% carbohydrate, and 20% protein caloric content (2014; Harlan Teklad, Madison, WI, USA) and had free access to food and water before randomization by weight. ..

Article Title: Fibroblast growth factor 21 and exercise-induced hepatic mitochondrial adaptations
Article Snippet: .. FGF21 knockout mice on a C57BL/6 background (FGF21-KO) and age-matched, but not littermate C57BL/6 WT mice controls were bred by Taconic Biosciences (Hudson, NY) and kindly provided by Eli Lilly (Indianapolis, IN). ..

Article Title: Anti-inflammatory Effects of Exercise Training in Adipose Tissue Do Not Require FGF21
Article Snippet: .. Male FGF21 knockout mice on a C57BL/6NTac background (FGF21KO) and age-matched, but not littermate, C57BL/6NTac WT mice controls were bred by Taconic Biosciences (Hudson, NY) and kindly provided by Eli Lilly (Indianapolis, IN). ..

other:

Article Title: Cholic Acid Supplementation of a High-Fat Obesogenic Diet Suppresses Hepatic Triacylglycerol Accumulation in Mice via a Fibroblast Growth Factor 21-Dependent Mechanism.
Article Snippet: Male FGF21KO mice (FGF21−/−) on the C57BL/6NTac background andWT (FGF21+/+) C57BL/6NTacmice were provided by Lilly Research Laboratories.This FGF21KO colony (Mouse Genome Informatics registered allele: fgf21tm1Emf) was developed by Taconic Biosciences under a contract with Lilly Research Laboratories.

Control:

Article Title: Fibroblast Growth Factor 21 Is Not Required for the Reductions in Circulating Insulin-Like Growth Factor-1 or Global Cell Proliferation Rates in Response to Moderate Calorie Restriction in Adult Mice
Article Snippet: 12- to 16-week-old male C57BL/6 mice were used in the initial studies (Jackson Laboratory, Bar Harbor, ME). .. For the FGF21-KO study, 12- to 17-week-old male control C57BL/6NTac (WT) mice and 12- to 15-week-old male FGF21-KO mice, generated as previously described , were used (Taconic Farms Inc., Hudson, NY). ..

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fgf21 knockout fgf21ko mice (Taconic Biosciences)

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