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R&D Systems fgf21
$<t>FGF21</t> is regulated by the nutritional status in fed, fasted and diet-induced obesity (DIO). Fasting reduced FGF21 mRNA in whole pancreas (A). β-klotho, the co-receptor of FGF21, is expressed in the pancreas and is inversely regulated by fasting (B). FGF21 protein was only detected in pancreas compared to liver and inguinal white adipose tissue (IWAT) (C). Pancreas FGF21 protein level was reduced by fasting in both chow fed and high fat diet fed conditions (D, E). Separation of pancreatic fractions to evaluate the individual contribution of islets and acinar tissue on FGF21 expression show that acinar pancreas contributes to the majority of FGF21 levels while islet cells have very low FGF21 levels and are not nutritionally regulated (F). Acinar expression of FGF21 is regulated with fasting (F, G). n = 6 per group. Each western blot lane represents an individual animal.$
Fgf21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fgf21/Mouse+FGF-21+Antibody/pmc04752212-46-6-8
Average 94 stars, based on 26 article reviews

fgf21 - by Bioz Stars, 2026-11

94/100 stars

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1) Product Images from "Fibroblast Growth Factor 21 (FGF21) Protects against High Fat Diet Induced Inflammation and Islet Hyperplasia in Pancreas"

Article Title: Fibroblast Growth Factor 21 (FGF21) Protects against High Fat Diet Induced Inflammation and Islet Hyperplasia in Pancreas

Journal: PLoS ONE

doi: 10.1371/journal.pone.0148252

$FGF21 is regulated by the nutritional status in fed, fasted and diet-induced obesity (DIO). Fasting reduced FGF21 mRNA in whole pancreas (A). β-klotho, the co-receptor of FGF21, is expressed in the pancreas and is inversely regulated by fasting (B). FGF21 protein was only detected in pancreas compared to liver and inguinal white adipose tissue (IWAT) (C). Pancreas FGF21 protein level was reduced by fasting in both chow fed and high fat diet fed conditions (D, E). Separation of pancreatic fractions to evaluate the individual contribution of islets and acinar tissue on FGF21 expression show that acinar pancreas contributes to the majority of FGF21 levels while islet cells have very low FGF21 levels and are not nutritionally regulated (F). Acinar expression of FGF21 is regulated with fasting (F, G). n = 6 per group. Each western blot lane represents an individual animal.$
Figure Legend Snippet: FGF21 is regulated by the nutritional status in fed, fasted and diet-induced obesity (DIO). Fasting reduced FGF21 mRNA in whole pancreas (A). β-klotho, the co-receptor of FGF21, is expressed in the pancreas and is inversely regulated by fasting (B). FGF21 protein was only detected in pancreas compared to liver and inguinal white adipose tissue (IWAT) (C). Pancreas FGF21 protein level was reduced by fasting in both chow fed and high fat diet fed conditions (D, E). Separation of pancreatic fractions to evaluate the individual contribution of islets and acinar tissue on FGF21 expression show that acinar pancreas contributes to the majority of FGF21 levels while islet cells have very low FGF21 levels and are not nutritionally regulated (F). Acinar expression of FGF21 is regulated with fasting (F, G). n = 6 per group. Each western blot lane represents an individual animal.

Techniques Used: Expressing, Western Blot

Acute FGF21 exposure induces ERK1/2 signaling events therefore phosphorylation of ERK1/2 (pERK1/2) has been chosen as a marker of FGF21 signaling. Up to 50% of acinar cells show strong nuclear pERK1/2 staining (brown) after FGF21 administration (A i, iii). Islets represent a very unique pattern with pERK1/2 only on the peripheral cells (A ii, iv). Percentage of pERK1/2-labeled acinar cells with saline and FGF21 administration (B). Western blot of pERK1/2 and total ERK1/2 from the same pancreas samples (C). Epididymal white adipose tissue (EWAT) is a positive control for FGF21 signaling (C). S = saline and F = FGF21. Confocal photomicrographs show FGF21-induced pERK1/2 immunoreactivity in islets (D). Absence of pERK1/2 staining (green) after saline treatment (Di; Inset , islet outline is indicated by insulin-immunoreactivity shown in red). FGF21 did not elicit pERK1/2 in insulin (red)-producing β-cells (D ii). Merged images show pERK1/2 co-localization in glucagon-positive α cells (D iii) and somatostatin-positive δ cell (D iv). Split panels show glucagon (iii a ) and pERK (iii b ) labeling or somatostatin (iv a ) and pERK (iv b ) labeling in single cells. A robust and specific pERK1/2 staining was observed in almost 10% of total islet nuclei mostly at the periphery. Outlined region (dashed line) in the inset indicates the region represented in each panel. Representative pERK1/2 labelled cells are indicated by white arrows. n = 4 per group. Scale: 200 μm, A; 20 μm, D.

Figure Legend Snippet: Acute FGF21 exposure induces ERK1/2 signaling events therefore phosphorylation of ERK1/2 (pERK1/2) has been chosen as a marker of FGF21 signaling. Up to 50% of acinar cells show strong nuclear pERK1/2 staining (brown) after FGF21 administration (A i, iii). Islets represent a very unique pattern with pERK1/2 only on the peripheral cells (A ii, iv). Percentage of pERK1/2-labeled acinar cells with saline and FGF21 administration (B). Western blot of pERK1/2 and total ERK1/2 from the same pancreas samples (C). Epididymal white adipose tissue (EWAT) is a positive control for FGF21 signaling (C). S = saline and F = FGF21. Confocal photomicrographs show FGF21-induced pERK1/2 immunoreactivity in islets (D). Absence of pERK1/2 staining (green) after saline treatment (Di; Inset , islet outline is indicated by insulin-immunoreactivity shown in red). FGF21 did not elicit pERK1/2 in insulin (red)-producing β-cells (D ii). Merged images show pERK1/2 co-localization in glucagon-positive α cells (D iii) and somatostatin-positive δ cell (D iv). Split panels show glucagon (iii a ) and pERK (iii b ) labeling or somatostatin (iv a ) and pERK (iv b ) labeling in single cells. A robust and specific pERK1/2 staining was observed in almost 10% of total islet nuclei mostly at the periphery. Outlined region (dashed line) in the inset indicates the region represented in each panel. Representative pERK1/2 labelled cells are indicated by white arrows. n = 4 per group. Scale: 200 μm, A; 20 μm, D.

Techniques Used: Phospho-proteomics, Marker, Staining, Labeling, Saline, Western Blot, Positive Control

Chronic FGF21 treatment for 3 days by mini osmotic pumps led to the reduction in FGF21 expression in pancreas (A). FGF21 infusion down-regulated insulin but glucagon, somatostatin or amylase expression. While FGF21 infusion did not alter serum glucose levels (B), it reduced the serum levels of pancreatic hormones, insulin (C) and glucagon (D) without affecting the amylase activity (E). n = 10 per group.

Figure Legend Snippet: Chronic FGF21 treatment for 3 days by mini osmotic pumps led to the reduction in FGF21 expression in pancreas (A). FGF21 infusion down-regulated insulin but glucagon, somatostatin or amylase expression. While FGF21 infusion did not alter serum glucose levels (B), it reduced the serum levels of pancreatic hormones, insulin (C) and glucagon (D) without affecting the amylase activity (E). n = 10 per group.

Techniques Used: Expressing, Activity Assay

WT and FGF21 KO mice on chow diet have similar body weight (A), glucose tolerance (B) and insulin secretion in response to glucose (C), despite notable differences in their pancreatic morphology (D i, iii). After consuming high fat diet for 16 weeks, FGF21 KO mice (D iv) had increased islet hyperplasia compared with WT mice (D ii). Islet cell surface area was measured and represented (E). Islet cell proliferation markers were substantially over expressed in obese FGF21 KO animals (F). Islet hyperplasia leads to increased serum insulin (G) in FGF21 KO animals with a slight reduction in blood glucose (H) (n = 7 per group). Scale: 1 mm.

Figure Legend Snippet: WT and FGF21 KO mice on chow diet have similar body weight (A), glucose tolerance (B) and insulin secretion in response to glucose (C), despite notable differences in their pancreatic morphology (D i, iii). After consuming high fat diet for 16 weeks, FGF21 KO mice (D iv) had increased islet hyperplasia compared with WT mice (D ii). Islet cell surface area was measured and represented (E). Islet cell proliferation markers were substantially over expressed in obese FGF21 KO animals (F). Islet hyperplasia leads to increased serum insulin (G) in FGF21 KO animals with a slight reduction in blood glucose (H) (n = 7 per group). Scale: 1 mm.

Techniques Used:

HFD consumption caused severe pancreatic inflammation in FGF21 KO mice (A). H&E staining showing, that compared to WT animals (i), FGF21 KO mice developed severe pancreatic periductal inflammation (outlined area in ii-iii) when consuming a HFD diet for 16 weeks. Lymphocytic nature of inflammatory cells is shown in a higher magnification image (iii). (B) Immunohistochemical analysis for the lymphocytic marker CD3 on the pancreas of WT (i) and FGF21 KO animals (ii-iii) is represented. FGF21 KO showed higher number of CD3, T cell receptor antigen. n = 7 per group. Scale: 1 mm, Ai-ii; 2 mm, Bi-ii; 200 μm, iii.

Figure Legend Snippet: HFD consumption caused severe pancreatic inflammation in FGF21 KO mice (A). H&E staining showing, that compared to WT animals (i), FGF21 KO mice developed severe pancreatic periductal inflammation (outlined area in ii-iii) when consuming a HFD diet for 16 weeks. Lymphocytic nature of inflammatory cells is shown in a higher magnification image (iii). (B) Immunohistochemical analysis for the lymphocytic marker CD3 on the pancreas of WT (i) and FGF21 KO animals (ii-iii) is represented. FGF21 KO showed higher number of CD3, T cell receptor antigen. n = 7 per group. Scale: 1 mm, Ai-ii; 2 mm, Bi-ii; 200 μm, iii.

Techniques Used: Staining, Immunohistochemical staining, Marker

(A) Representative cytofluorometric dot plots of isolated lymphocytes from WT and FGF21 KO animals consuming high fat diet for 16 weeks. FGF21 KO mice show elevated CD45+ (i), TCRb+ and Thy1+ T lymphocytes (ii) and Foxp3+ Treg cells. CD19+ B lymphocytes were not significantly altered (iii). Corresponding summary data is shown in the right panel. The experiment was repeated twice (n = 4 per group). (B) Gene expression analysis of cytokines is presented. n = 7 per group.

Figure Legend Snippet: (A) Representative cytofluorometric dot plots of isolated lymphocytes from WT and FGF21 KO animals consuming high fat diet for 16 weeks. FGF21 KO mice show elevated CD45+ (i), TCRb+ and Thy1+ T lymphocytes (ii) and Foxp3+ Treg cells. CD19+ B lymphocytes were not significantly altered (iii). Corresponding summary data is shown in the right panel. The experiment was repeated twice (n = 4 per group). (B) Gene expression analysis of cytokines is presented. n = 7 per group.

Techniques Used: Isolation, Gene Expression

Magnetic Resonance Imaging:

Article Title: An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarction
Article Snippet: The protein bands were visualized with an ECL detection kit (Thermo Fisher Scientific) and quantified using ImageJ software. .. The following primary antibodies were used: MR (1:500; ab41912, Abcam), FGF21 (1:1000; AF3057, R&D Systems), cleaved caspase-3 (1:1000; 9664S, Cell Signaling Technology), NCOR1 (1:500; 5948S, Cell Signaling Technology), anti-FLAG (1:2000; F1804, Sigma-Aldrich), HDAC1 (1:500; 39531, Active Motif), HDAC3 (1:1000; 85057S, Cell Signaling Technology), HDAC5 (1:1000; ab55403, Abcam), HDAC6 (1:500; 7612S, Cell Signaling Technology), HDAC9 (1:500; ab59718, Abcam), p-STAT3 (1:1000; 9145S, Cell Signaling Technology), STAT3 (1:1000; 9139S, Cell Signaling Technology,), α-tubulin (1:5000; T6199, Sigma-Aldrich), and GAPDH (1:5000; 5174S, Cell Signaling Technology). .. Mouse livers were preserved in RNAlater and sent to WuXi NextCODE (Shanghai, China) for sequencing.

Cell-Signaling:

Ubiquitin Proteomics:

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner.
Article Snippet: In addition, InstantBlue Coomassie protein stain was performed according to according to manufacturer’s instructions (Abcam, Cat# ab119211) to visualize protein loading of the ECM fractions. .. Primary antibodies used in this study were: Akt (Cell Signaling Technology, Cat# 9272 at 1:1000), p-Akt Ser473 (Cell Signaling Technology, Cat# 4060 at 1:1000), AMPKa (Cell Signaling Technology, Cat# 2532 at 1:1000), p-AMPKa Thr172 (Cell Signaling Technology, Cat# 2535 at 1:1000), ATF4 (Cell Signaling Technology, Cat# 11815 and LSBio LifeSpan Biosciences, Cat# LS-B6361; both at 1:1000), ATF6a (SAB SignalWayAntibody LLC, Cat# 24383; at 1:1000), BiP (Sigma, Cat# GG8918; at 1:1000), calreticulin (Cell Signaling Technology, Cat# 2891; at 1:1000), b-dystroglycan (developmental Studies Hybridoma Bank, cat# MANDAG2 clone 7D11; 1:100), p-4E-BP1 Thr37/46 (Cell Signaling Technology, Cat# 2855 at 1:1000), 4E-BP1 (Cell Signaling Technology, Cat# 9644 at 1:1000), FGF21 (R&D Systems, Cat# AF3057; at 1:500), Foxo1 (Cell Signaling Technology, Cat# 2880 at 1:1000), p-Foxo1 ser256 (Cell Signaling Technology, Cat# 9461 at 1:1000), Foxo3 (Cell Signaling Technology, Cat# 2497 at 1:1000), p-Foxo3 ser413 (Cell Signaling Technology, Cat# 8174 at 1:1000), p-Foxo3 ser 253 (Cell Signaling Technology, Cat# 9466 at 1:1000), IRE1a (Cell Signaling Technology, Cat# 3294; at 1:1000), b1D-integrin (Millipore, MAB1900; at 1:500), LAMP1 (Development Studies HybridomaBank, Cat# 1D4B; at 1:100), LAMP2 (Development Studies HybridomaBank, Cat# ABL-93; at 1:100), LC3b (Cell Signaling Technology, Cat# 3868; at 1:1000), mTOR (Cell Signaling Technology, Cat# 2972 at 1:1000), p-mTOR ser2448 (Cell Signaling Technology, Cat# 2971 at 1:1000), p62 (Sigma, Cat# p0067 at 1:1000), LIMPII (Abcam, Cat# ab176317; at 1:1000), p-p70 S6K Thr389 (Cell Signaling Technology, Cat# 9205 at 1:1000), p70 S6K (Cell Signaling Technology, Cat# 2708 at 1:1000),PERK (Cell Signaling Technology, Cat# 3192; at 1:1000), anti-puromycin (Sigma, Cat# MABE343; at 1:1000); a-sarcoglycan (Development Studies Hybridoma Bank, Cat# IVD3(1)A9; at 1:100), b-sarcoglycan (Novus Biologicals, Cat# NBP1-90300; at 1:1000); d-sarcoglycan (Abcam, Cat# ab137101; at 1:500); Smad2 (Cell Signaling Technology, Cat# 5339; at 1:1000); Smad3 (Abcam, Cat# ab40854; at 1:500); P-Smad2 Ser465/467/P-Smad3 Ser423/425 (Signaling Technology, Cat# 8828; at 1:1000); p-Smad3 Ser423/425 (Signaling Technology, Cat# 9520; at 1:1000); TGFb RII (Santa Cruz Biotechnology, Cat# sc-17799; at 1:200); Thbs1 (R&D Systems, Cat# AF3074; at 1:500 and ThermoFisher Scientific, Cat# MA5-13395; at 1:1000); Thbs2 (R&D Systems, Cat# MAB1635; at 1:500); Thbs4 (R&D Systems, Cat# AF2390; at 1:500); Ubiquitin (Santa Cruz Biotechnology, Cat# sc-8017; at 1:200); and glyceraldehyde 3-phosphate dehydrogenase (Gapdh; Fitzgerald, Cat# 10R-G109A; at 1:10000) which served as overall loading control. .. Transmission electron microscopy was performed as previously described.24 In short, quadriceps from anesthetized mice were harvested and immediately immersed in relaxing buffer (0.15% sucrose, 5% dextrose, 100 mM KCl in 1x PBS), fixed overnight (3.5% glutaraldehyde, 0.15% sucrose in 0.1 M sodium cacodylate ph 7.4), post-fixed for 2 h at room temperature in 1% OsO4 (in water), washed, dehydrated and embedded using Epoxy resin (Electron Microscopy Sciences, Embed 812 Embedding Kit with BDMA, 22 Cell Reports 43, 114149, May 28, 2024 Cat# 14121).

Control:

Bioprocessing:

Article Title: MAPL loss dysregulates bile and liver metabolism in mice.
Article Snippet: Bands were visualized with Western-Lightning PLUS-ECL (Perkin-Elmer) with an INTAS ChemoCam (INTAS Science Imaging GmbH) and quantified with ImageJ software. .. Mitochondrial and peroxisomal anchored protein ligase was detected by rabbit polyclonal antibodies (HPA017681, 1:1,000, Sigma), PERK by rabbit polyclonal antibodies (100-401-962, 1:1,000, Rockland antibodies & assays), phospho-PERK by rabbit monoclonal antibodies (3179, 1:1,000, Cell Signaling), eIF2a by mouse monoclonal antibodies (2103, 1:500, Cell Signaling), phosphoeIF2a by rabbit polyclonal antibodies (SAB4504388, 1:500, Sigma), Fgf21 by goat polyclonal antibodies (AF3057, 1:500, R&D systems), CHOP by rabbit polyclonal antibodies (5554, 1:500, Cell Signaling), BiP by rabbit polyclonal antibodies (ADI-SPA-826-D, 1:1,000, Enzo), CYP3A11 by rabbit polyclonal antibodies (13384, 1:500, Cell Signaling), CYP4A14 by goat polyclonal antibodies (sc-46087, 1:500, Santa Cruz), CYP7A1 by rabbit polyclonal antibodies (ab65596, 1:500, Abcam), CYP27A1 by rabbit polyclonal antibodies (NBP2-16061, 1:500, Novus Biologicals), SUMO1 by mouse monoclonal antibodies (332400, 1:1,000, Invitrogen), Hsp60 by mouse monoclonal antibodies (sc-136291, 1:1,000, Santa Cruz), Hsp70 by rabbit polyclonal antibodies (ab137680, 1:1,000, Abcam), ABCD3 by mouse monoclonal antibodies (sab4200181, 1:1,000, Sigma), DRP1 by mouse monoclonal antibodies (611113, 1:1,000, BD Transduction Labs), Mfn2 by rabbit polyclonal antibodies (M6319, 1:1,000, Sigma), UCP1 by a polyclonal antibody (U6382, 1:500, Sigma), ACOX1 by a polyclonal antibody (10957-1-AP, 1:1,000, Proteintech), SCP2 by a polyclonal antibody (14377-1-AP, 1:1,000, Proteintech), PEX14 by a polyclonal antibody (ABC142, 1:1,000, Millipore), vinculin by a monoclonal antibody (V4505, 1:1,000, Sigma), b-actin by rabbit polyclonal antibodies (SAB4502543, 1:1,000, Sigma) AIF by mouse monoclonal antibody (sc13116 1:1,000, SantaCruz), ABCB11 by polyclonal antibodies (PA527742 1:1,000, Thermo Fisher), EGFR by rabbit polyclonal antibodies (A00023 1:1,000, Boster), phospho-EGFR by rabbit polyclonal antibodies (369700 1:1,000, Invitrogen) and b-actin by mouse monoclonal antibodies (A2228, 1:1,000, Sigma). ..

Article Title: MAPL loss dysregulates bile and liver metabolism in mice
Article Snippet: Bands were visualized with Western‐Lightning PLUS‐ECL (Perkin‐Elmer) with an INTAS ChemoCam (INTAS Science Imaging GmbH) and quantified with ImageJ software. .. Mitochondrial and peroxisomal anchored protein ligase was detected by rabbit polyclonal antibodies (HPA017681, 1:1,000, Sigma), PERK by rabbit polyclonal antibodies (100‐401‐962, 1:1,000, Rockland antibodies & assays), phospho‐PERK by rabbit monoclonal antibodies (3179, 1:1,000, Cell Signaling), eIF2α by mouse monoclonal antibodies (2103, 1:500, Cell Signaling), phospho‐eIF2α by rabbit polyclonal antibodies (SAB4504388, 1:500, Sigma), Fgf21 by goat polyclonal antibodies (AF3057, 1:500, R&D systems), CHOP by rabbit polyclonal antibodies (5554, 1:500, Cell Signaling), BiP by rabbit polyclonal antibodies (ADI‐SPA‐826‐D, 1:1,000, Enzo), CYP3A11 by rabbit polyclonal antibodies (13384, 1:500, Cell Signaling), CYP4A14 by goat polyclonal antibodies (sc‐46087, 1:500, Santa Cruz), CYP7A1 by rabbit polyclonal antibodies (ab65596, 1:500, Abcam), CYP27A1 by rabbit polyclonal antibodies (NBP2‐16061, 1:500, Novus Biologicals), SUMO1 by mouse monoclonal antibodies (332400, 1:1,000, Invitrogen), Hsp60 by mouse monoclonal antibodies (sc‐136291, 1:1,000, Santa Cruz), Hsp70 by rabbit polyclonal antibodies (ab137680, 1:1,000, Abcam), ABCD3 by mouse monoclonal antibodies (sab4200181, 1:1,000, Sigma), DRP1 by mouse monoclonal antibodies (611113, 1:1,000, BD Transduction Labs), Mfn2 by rabbit polyclonal antibodies (M6319, 1:1,000, Sigma), UCP1 by a polyclonal antibody (U6382, 1:500, Sigma), ACOX1 by a polyclonal antibody (10957‐1‐AP, 1:1,000, Proteintech), SCP2 by a polyclonal antibody (14377‐1‐AP, 1:1,000, Proteintech), PEX14 by a polyclonal antibody (ABC142, 1:1,000, Millipore), vinculin by a monoclonal antibody (V4505, 1:1,000, Sigma), β‐actin by rabbit polyclonal antibodies (SAB4502543, 1:1,000, Sigma) AIF by mouse monoclonal antibody (sc13116 1:1,000, SantaCruz), ABCB11 by polyclonal antibodies (PA527742 1:1,000, Thermo Fisher), EGFR by rabbit polyclonal antibodies (A00023 1:1,000, Boster), phospho‐EGFR by rabbit polyclonal antibodies (369700 1:1,000, Invitrogen) and β‐actin by mouse monoclonal antibodies (A2228, 1:1,000, Sigma). ..

Incubation:

Article Title: Fibroblast growth factor 21 ameliorates cholestatic liver injury via a hepatic FGFR4-JNK pathway.
Article Snippet: Cholestasis is characterized by hepatic accumulation of cytotoxic bile acids (BAs), which often subsequently leads to liver injury, inflammation, fibrosis, and liver cirrhosis.. Fibroblast growth factor 21 (FGF21) is a liversecreted hormone with pleiotropic effects on the homeostasis of glucose, lipid, and energy metabolism.. However, whether hepatic FGF21 plays a role in cholestatic liver injury remains elusive.