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serum fgf21  (R&D Systems)


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    Structured Review

    R&D Systems serum fgf21
    Serum Fgf21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/Mouse%2FRat+FGF-21+Quantikine+ELISA+Kit/pm41812727-85-0-3
    Average 96 stars, based on 294 article reviews
    serum fgf21 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Modification:

    Article Title: Target to treat a metabolic disease in an individual
    Article Snippet: .. Evaluation of the effect of ION No. 1018060 on FGF-21 levels was evaluated 48 hours post final dose Measurement of FGF-21 Levels To evaluate the effect of modified oligonucleotides on liver function, FGF-21 levels were measured in liver homogenates using an R&D Systems Quantikine ELISA kit (MF2100). ..

    Article Title: Target to treat a metabolic disease in an individual
    Article Snippet: .. Measurement of FGF-21 levels To evaluate the effect of modified oligonucleotides on liver function, FGF-21 levels were measured in liver homogenates using an R&D Systems Quantikine ELISA kit (MF2100). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Target to treat a metabolic disease in an individual
    Article Snippet: .. Evaluation of the effect of ION No. 1018060 on FGF-21 levels was evaluated 48 hours post final dose Measurement of FGF-21 Levels To evaluate the effect of modified oligonucleotides on liver function, FGF-21 levels were measured in liver homogenates using an R&D Systems Quantikine ELISA kit (MF2100). ..

    Article Title: Target to treat a metabolic disease in an individual
    Article Snippet: .. Measurement of FGF-21 levels To evaluate the effect of modified oligonucleotides on liver function, FGF-21 levels were measured in liver homogenates using an R&D Systems Quantikine ELISA kit (MF2100). ..

    Article Title: Fibroblast growth factor 21-inducing rare sugars prevent diet-induced obesity in male BL/6 mice
    Article Snippet: .. Mouse FGF21 protein levels were quantified using the Mouse/Rat FGF-21 Quantikine ELISA Kit (MF2100; R & D Systems) according to the instructions of the manufacturer. .. Data visualization and statistical analysis All analyses were conducted using Microsoft Excel and GraphPad Prism 10.

    Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice
    Article Snippet: Plasma insulin levels were determined using an ultra-sensitive mouse insulin ELISA kit (90080) from Crystal Chem (Elk Grove Village, IL, USA). .. Blood for circulating FGF21, testosterone, and estradiol levels was collected from submandibular bleeding immediately prior to euthanasia; plasma was assayed with a mouse/rat FGF21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA), and testosterone (582701) and estradiol (501890) quantikine ELISA kits from Caymen Chemicals (Ann Arbor, MI, USA). .. Liver and plasma triglycerides and cholesterol were measured using Triglyceride Colorimetric Assay Kit (Pointe Scientific, #C75101L) and Cholesterol Colorimetric Assay Kit (Pointe Scientific, T7532500) following the manufacturer’s protocol.

    Article Title: Rab2A modulates liver fibroblast growth factor 21 (FGF21) expression and systemic metabolism via apolipoprotein B–CREBH signaling
    Article Snippet: .. Circulating levels of FGF21 were measured using ELISA kits (R&D Systems; MF2100). ..

    Article Title: Inducible Impairment of Polymerase Gamma Activity in Cardiomyocytes Promotes Severe Cardiomyopathy with Cardiac Hepatopathy
    Article Snippet: Image Lab Software (BioRad) was used to perform densitometry analyses, and all quantification results were normalized to their respective loading control or total protein. .. Commercial ELISA kits for FGF21 (R&D Systems, #MF2100) and GDF15 (R&D Systems, #MGD150) were used to measure respective plasma concentration as per manufacturer’s instructions. .. Mouse heart tissue (20-, 24- & 28-week PolG Con /PolG Mut , n=5/group/time-point) were lysed on ice (8M urea in 50 mM HEPES pH 8.0 with HaltTM Protease/Phosphatase Inhibitor Cocktail, #78442, Thermo Fisher Scientific) and extracted by rapid pulse tip-probe sonication on ice.

    Article Title: Methionine restriction provides sex-specific protection against high-fat diet-induced adiposity, peripheral insulin resistance, and neuroinflammation in FGF21-dependent and independent manners in mice
    Article Snippet: .. Serum FGF21 concentrations were measured using an enzyme-linked immunosorbent assay (ELISA), according to the solid phase sandwich method (Cat# MF2100; R&D Systems, Minneapolis, MN) following the manufacturer’s protocol. ..

    Clinical Proteomics:

    Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice
    Article Snippet: Plasma insulin levels were determined using an ultra-sensitive mouse insulin ELISA kit (90080) from Crystal Chem (Elk Grove Village, IL, USA). .. Blood for circulating FGF21, testosterone, and estradiol levels was collected from submandibular bleeding immediately prior to euthanasia; plasma was assayed with a mouse/rat FGF21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA), and testosterone (582701) and estradiol (501890) quantikine ELISA kits from Caymen Chemicals (Ann Arbor, MI, USA). .. Liver and plasma triglycerides and cholesterol were measured using Triglyceride Colorimetric Assay Kit (Pointe Scientific, #C75101L) and Cholesterol Colorimetric Assay Kit (Pointe Scientific, T7532500) following the manufacturer’s protocol.

    Article Title: Inducible Impairment of Polymerase Gamma Activity in Cardiomyocytes Promotes Severe Cardiomyopathy with Cardiac Hepatopathy
    Article Snippet: Image Lab Software (BioRad) was used to perform densitometry analyses, and all quantification results were normalized to their respective loading control or total protein. .. Commercial ELISA kits for FGF21 (R&D Systems, #MF2100) and GDF15 (R&D Systems, #MGD150) were used to measure respective plasma concentration as per manufacturer’s instructions. .. Mouse heart tissue (20-, 24- & 28-week PolG Con /PolG Mut , n=5/group/time-point) were lysed on ice (8M urea in 50 mM HEPES pH 8.0 with HaltTM Protease/Phosphatase Inhibitor Cocktail, #78442, Thermo Fisher Scientific) and extracted by rapid pulse tip-probe sonication on ice.

    Concentration Assay:

    Article Title: Inducible Impairment of Polymerase Gamma Activity in Cardiomyocytes Promotes Severe Cardiomyopathy with Cardiac Hepatopathy
    Article Snippet: Image Lab Software (BioRad) was used to perform densitometry analyses, and all quantification results were normalized to their respective loading control or total protein. .. Commercial ELISA kits for FGF21 (R&D Systems, #MF2100) and GDF15 (R&D Systems, #MGD150) were used to measure respective plasma concentration as per manufacturer’s instructions. .. Mouse heart tissue (20-, 24- & 28-week PolG Con /PolG Mut , n=5/group/time-point) were lysed on ice (8M urea in 50 mM HEPES pH 8.0 with HaltTM Protease/Phosphatase Inhibitor Cocktail, #78442, Thermo Fisher Scientific) and extracted by rapid pulse tip-probe sonication on ice.



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    R&D Systems mouse rat fgf21 quantikine elisa kit
    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
    Mouse Rat Fgf21 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/Mouse%2FRat+FGF-21+Quantikine+ELISA+Kit/bio_rxiv__64898__2026__03__31__715667-55-22-29
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    Proteintech c plasma fgf21 protein levels
    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
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    Image Search Results


    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).

    Journal: bioRxiv

    Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice

    doi: 10.64898/2026.03.31.715667

    Figure Lengend Snippet: (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).

    Article Snippet: Blood for circulating FGF21, testosterone, and estradiol levels was collected from submandibular bleeding immediately prior to euthanasia; plasma was assayed with a mouse/rat FGF21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA), and testosterone (582701) and estradiol (501890) quantikine ELISA kits from Caymen Chemicals (Ann Arbor, MI, USA).

    Techniques: Activity Assay, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control