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human intact fgf21 elisa kit  (Eagle Biosciences)


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    Eagle Biosciences human intact fgf21 elisa kit
    Human Intact Fgf21 Elisa Kit, supplied by Eagle Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf21/Intact+FGF-21+ELISA/pm40929158-52-19-25
    Average 93 stars, based on 21 article reviews
    human intact fgf21 elisa kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: FGF21 variants
    Article Snippet: In detail, mature human FGF21 (SEQ ID NO: 2) or variants were added to blood samples (K2EDTA plasma, mixture of several healthy human donors) at a concentration of 100 ng/mL and incubated for up to 24 hours at 37° C. After 0, 1, 8, and/or 24 hours, samples were taken, and incubation was stopped by adding 2× concentrated protease inhibitor (Protease Inhibitor Cocktail Tablets, Roche). .. Subsequently, the amount of remaining intact, full length FGF21 protein was assessed using Human Intact FGF21 ELISA (F2131-K01, Eagle Biosciences, Nashua, N.H., USA). .. Subsequently, the amount of remaining intact, full length FGF21 protein was assessed using Human Intact FGF21 ELISA (F2131-K01, Eagle Biosciences, Nashua, N.H., USA).

    Article Title: Increased Fibrosis in White Adipose Tissue of Male and Female bGH Transgenic Mice Appears Independent of TGF-β Action.
    Article Snippet: Fibrosis is a pathological state caused by excess deposition of extracellular matrix proteins in a tissue.. Male bovine growth hormone (bGH) transgenic mice experience metabolic dysfunction with a marked decrease in lifespan and with increased fibrosis in several tissues including white adipose tissue (WAT), which is more pronounced in the subcutaneous (Sc) depot.. The current study expanded on these initial findings to evaluate WAT fibrosis in female bGH mice and the role of transforming growth factor (TGF)-β in the development of WAT fibrosis.

    Article Title: Protein intake counteracts alcohol intake in the regulation of postprandial FGF21 secretion in humans.
    Article Snippet: .. 140 Laboratory analyses 141 Concentrations of intact human FGF21 were measured in duplicate in plasma samples using 142 the Human Intact FGF21 ELISA kit (F2131-K01, Eagle Biosciences, Nashua, NH) according 143 to the manufacturer’s instructions. ..

    Clinical Proteomics:

    Article Title: Meal sugar-protein balance determines postprandial FGF21 response in humans
    Article Snippet: .. Intact FGF21 was measured in duplicate in EDTA plasma samples by enzyme-linked immunosorbent assay (ELISA) (Eagle Biosciences, F2131-K01) in accordance with the manufacturer’s instructions. ..

    Article Title: Protein intake counteracts alcohol intake in the regulation of postprandial FGF21 secretion in humans.
    Article Snippet: .. 140 Laboratory analyses 141 Concentrations of intact human FGF21 were measured in duplicate in plasma samples using 142 the Human Intact FGF21 ELISA kit (F2131-K01, Eagle Biosciences, Nashua, NH) according 143 to the manufacturer’s instructions. ..



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    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
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    Proteintech c plasma fgf21 protein levels
    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
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    Image Search Results


    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).

    Journal: bioRxiv

    Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice

    doi: 10.64898/2026.03.31.715667

    Figure Lengend Snippet: (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).

    Article Snippet: Blood for circulating FGF21, testosterone, and estradiol levels was collected from submandibular bleeding immediately prior to euthanasia; plasma was assayed with a mouse/rat FGF21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA), and testosterone (582701) and estradiol (501890) quantikine ELISA kits from Caymen Chemicals (Ann Arbor, MI, USA).

    Techniques: Activity Assay, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control