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U.S Biomax Inc ffpe oscc tissue microarray
Immunohistochemical analysis using C 44 Mab-108 and C 44 Mab-46 against oral squamous cell carcinoma <t>(OSCC)</t> tissues. After antigen retrieval, the sections were incubated with 10 µg/mL of C 44 Mab-108 ( A , B , I , J ), 1 µg/mL of C 44 Mab-46 ( C , D , K , L ), and without the primary antibody (control) ( E , F , M , N ) followed by treatment with the Envision+ kit. The color was developed using 3,3′-diaminobenzidine tetrahydrochloride (DAB), and the sections were counterstained with hematoxylin. ( G , H , O , P ) Hematoxylin and eosin (HE) staining. ( Q , R ) Blocking of the C 44 Mab-108 reactivity to OSCC tissue by the CD44 peptide (aa 271–290) containing the C 44 Mab-108 epitope. After antigen retrieval, sections were incubated with C 44 Mab-108 (10 μg/mL) or C 44 Mab-108 (10 μg/mL) plus human CD44 peptide (aa 271–290, 10 μg/mL) followed by treatment with the Envision+ kit. The color was developed using DAB, and sections were counterstained with hematoxylin. Scale bar = 100 µm.
Ffpe Oscc Tissue Microarray, supplied by U.S Biomax Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ffpe+oscc+tissue+microarray/pmc10047040-135-3-10
Average 92 stars, based on 1 article reviews
ffpe oscc tissue microarray - by Bioz Stars, 2026-10
92/100 stars

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Article Title: Development of a Novel Anti-CD44 Variant 4 Monoclonal Antibody C 44 Mab-108 for Immunohistochemistry

Journal: Current Issues in Molecular Biology

doi: 10.3390/cimb45030121

Immunohistochemical analysis using C 44 Mab-108 and C 44 Mab-46 against oral squamous cell carcinoma (OSCC) tissues. After antigen retrieval, the sections were incubated with 10 µg/mL of C 44 Mab-108 ( A , B , I , J ), 1 µg/mL of C 44 Mab-46 ( C , D , K , L ), and without the primary antibody (control) ( E , F , M , N ) followed by treatment with the Envision+ kit. The color was developed using 3,3′-diaminobenzidine tetrahydrochloride (DAB), and the sections were counterstained with hematoxylin. ( G , H , O , P ) Hematoxylin and eosin (HE) staining. ( Q , R ) Blocking of the C 44 Mab-108 reactivity to OSCC tissue by the CD44 peptide (aa 271–290) containing the C 44 Mab-108 epitope. After antigen retrieval, sections were incubated with C 44 Mab-108 (10 μg/mL) or C 44 Mab-108 (10 μg/mL) plus human CD44 peptide (aa 271–290, 10 μg/mL) followed by treatment with the Envision+ kit. The color was developed using DAB, and sections were counterstained with hematoxylin. Scale bar = 100 µm.
Figure Legend Snippet: Immunohistochemical analysis using C 44 Mab-108 and C 44 Mab-46 against oral squamous cell carcinoma (OSCC) tissues. After antigen retrieval, the sections were incubated with 10 µg/mL of C 44 Mab-108 ( A , B , I , J ), 1 µg/mL of C 44 Mab-46 ( C , D , K , L ), and without the primary antibody (control) ( E , F , M , N ) followed by treatment with the Envision+ kit. The color was developed using 3,3′-diaminobenzidine tetrahydrochloride (DAB), and the sections were counterstained with hematoxylin. ( G , H , O , P ) Hematoxylin and eosin (HE) staining. ( Q , R ) Blocking of the C 44 Mab-108 reactivity to OSCC tissue by the CD44 peptide (aa 271–290) containing the C 44 Mab-108 epitope. After antigen retrieval, sections were incubated with C 44 Mab-108 (10 μg/mL) or C 44 Mab-108 (10 μg/mL) plus human CD44 peptide (aa 271–290, 10 μg/mL) followed by treatment with the Envision+ kit. The color was developed using DAB, and sections were counterstained with hematoxylin. Scale bar = 100 µm.

Techniques Used: Immunohistochemical staining, Incubation, Staining, Blocking Assay

Related Articles

other:

Article Title: Development of a Novel Anti-CD44 Variant 4 Monoclonal Antibody C 44 Mab-108 for Immunohistochemistry
Article Snippet: The formalin-fixed paraffin-embedded (FFPE) OSCC tissue microarray (Product Code: OR601c, US Biomax Inc., Rockville, MD, USA) and the esophageal tissue microarray (Product Code: BC02011, US Biomax Inc.) were deparaffinized in xylene (Sigma-Aldrich Corp.) and rehydrated.

Formalin-fixed Paraffin-Embedded:

Article Title: Development of a Novel Anti-CD44 Variant 8 Monoclonal Antibody C<sub>44</sub>Mab-94 against Gastric Carcinomas
Article Snippet: .. Formalin-fixed paraffin-embedded (FFPE) gastric carcinoma (BS01012e and BS01011b) and OSCC (OR601c) tissue arrays were obtained from US Biomax Inc. (Rockville, MD, USA). .. The tissue arrays were autoclaved in citrate buffer (pH 6.0; Nichirei Biosciences, Inc., Tokyo, Japan) for 20 min.

Article Title: A Novel Anti-CD44 Variant 3 Monoclonal Antibody C 44 Mab-6 Was Established for Multiple Applications.
Article Snippet: .. FFPE sections of OSCC tissue array (OR601c) and colorectal carcinoma tissue array (CO483a) were purchased from US Biomax Inc. (Rockville, MD, USA). .. The tissue arrays were autoclaved in EnVision FLEX Target Retrieval Solution High pH (Agilent Technologies, Inc.) for 20 min. After blocking with SuperBlock T20 (Thermo Fisher Scientific, Inc.), the sections were incubated with C44Mab-6 (1 μg/mL) and C44Mab-46 (1 μg/mL) for 1 h at room temperature.

Article Title: Development of a Novel Anti-CD44 Variant 7/8 Monoclonal Antibody C44Mab-34 for Multiple Applications against Oral Carcinomas
Article Snippet: The chemiluminescence signals were obtained with ImmunoStar LD (FUJIFILM Wako Pure Chemical Corporation), and detected using a Sayaca-Imager (DRC Co. Ltd., Tokyo, Japan). .. Formalin-fixed paraffin-embedded (FFPE) sections of the OSCC tissue array (Catalog number: OR601c) were purchased from US Biomax Inc. (Rockville, MD, USA). .. The OSCC tissue array was autoclaved in EnVision FLEX Target Retrieval Solution High pH (Agilent Technologies, Inc.) for 20 min. After blocking with SuperBlock T20 (Thermo Fisher Scientific, Inc.), the sections were incubated with C44Mab-34 (10 μg/mL) and C44Mab-46 (1 μg/mL) for 1 h at room temperature and then treated with the EnVision+ Kit for mouse (Agilent Technologies Inc.) for 30 min.

Microarray:

Article Title: Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression
Article Snippet: .. This is a cross-sectional, descriptive observational study using tissue microarray (TMA OR601c, US Biomax Inc., Rockville, MD, USA) containing 50 samples of OSCC and 10 samples of healthy oral mucosa (OM) used as control. ..

Control:

Article Title: Key proteins of invadopodia are overexpressed in oral squamous cell carcinoma suggesting an important role of MT1-MMP in the tumoral progression
Article Snippet: .. This is a cross-sectional, descriptive observational study using tissue microarray (TMA OR601c, US Biomax Inc., Rockville, MD, USA) containing 50 samples of OSCC and 10 samples of healthy oral mucosa (OM) used as control. ..

Immunohistochemical staining:

Article Title: Interleukin 17 and peripheral IL-17-expressing T cells are negatively correlated with the overall survival of head and neck cancer patients
Article Snippet: Samples were run on BD FACSCalibur, and FCS ExpressV3 Software (BD Biosciences) was used for the analysis. .. Immunohistochemical staining for human IL-17RA was performed using a human oral squamous cell cancer tissue array with adjacent normal tongue tissue (US Biomax Inc., cat. OR601a). .. For deparaffinization, rehydration and antigen retrieval, the sections were immersed in TrilogyTM solution (Sigma–Aldrich) and autoclaved at 120°C for 20 min.

Staining:

Article Title: Interleukin 17 and peripheral IL-17-expressing T cells are negatively correlated with the overall survival of head and neck cancer patients
Article Snippet: Samples were run on BD FACSCalibur, and FCS ExpressV3 Software (BD Biosciences) was used for the analysis. .. Immunohistochemical staining for human IL-17RA was performed using a human oral squamous cell cancer tissue array with adjacent normal tongue tissue (US Biomax Inc., cat. OR601a). .. For deparaffinization, rehydration and antigen retrieval, the sections were immersed in TrilogyTM solution (Sigma–Aldrich) and autoclaved at 120°C for 20 min.



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U.S Biomax Inc ffpe oscc tissue microarray
Immunohistochemical analysis using C 44 Mab-108 and C 44 Mab-46 against oral squamous cell carcinoma <t>(OSCC)</t> tissues. After antigen retrieval, the sections were incubated with 10 µg/mL of C 44 Mab-108 ( A , B , I , J ), 1 µg/mL of C 44 Mab-46 ( C , D , K , L ), and without the primary antibody (control) ( E , F , M , N ) followed by treatment with the Envision+ kit. The color was developed using 3,3′-diaminobenzidine tetrahydrochloride (DAB), and the sections were counterstained with hematoxylin. ( G , H , O , P ) Hematoxylin and eosin (HE) staining. ( Q , R ) Blocking of the C 44 Mab-108 reactivity to OSCC tissue by the CD44 peptide (aa 271–290) containing the C 44 Mab-108 epitope. After antigen retrieval, sections were incubated with C 44 Mab-108 (10 μg/mL) or C 44 Mab-108 (10 μg/mL) plus human CD44 peptide (aa 271–290, 10 μg/mL) followed by treatment with the Envision+ kit. The color was developed using DAB, and sections were counterstained with hematoxylin. Scale bar = 100 µm.
Ffpe Oscc Tissue Microarray, supplied by U.S Biomax Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ffpe+oscc+tissue+microarray/pmc10047040-135-3-10
Average 92 stars, based on 1 article reviews
ffpe oscc tissue microarray - by Bioz Stars, 2026-10
92/100 stars
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Immunohistochemical analysis using C 44 Mab-108 and C 44 Mab-46 against oral squamous cell carcinoma (OSCC) tissues. After antigen retrieval, the sections were incubated with 10 µg/mL of C 44 Mab-108 ( A , B , I , J ), 1 µg/mL of C 44 Mab-46 ( C , D , K , L ), and without the primary antibody (control) ( E , F , M , N ) followed by treatment with the Envision+ kit. The color was developed using 3,3′-diaminobenzidine tetrahydrochloride (DAB), and the sections were counterstained with hematoxylin. ( G , H , O , P ) Hematoxylin and eosin (HE) staining. ( Q , R ) Blocking of the C 44 Mab-108 reactivity to OSCC tissue by the CD44 peptide (aa 271–290) containing the C 44 Mab-108 epitope. After antigen retrieval, sections were incubated with C 44 Mab-108 (10 μg/mL) or C 44 Mab-108 (10 μg/mL) plus human CD44 peptide (aa 271–290, 10 μg/mL) followed by treatment with the Envision+ kit. The color was developed using DAB, and sections were counterstained with hematoxylin. Scale bar = 100 µm.

Journal: Current Issues in Molecular Biology

Article Title: Development of a Novel Anti-CD44 Variant 4 Monoclonal Antibody C 44 Mab-108 for Immunohistochemistry

doi: 10.3390/cimb45030121

Figure Lengend Snippet: Immunohistochemical analysis using C 44 Mab-108 and C 44 Mab-46 against oral squamous cell carcinoma (OSCC) tissues. After antigen retrieval, the sections were incubated with 10 µg/mL of C 44 Mab-108 ( A , B , I , J ), 1 µg/mL of C 44 Mab-46 ( C , D , K , L ), and without the primary antibody (control) ( E , F , M , N ) followed by treatment with the Envision+ kit. The color was developed using 3,3′-diaminobenzidine tetrahydrochloride (DAB), and the sections were counterstained with hematoxylin. ( G , H , O , P ) Hematoxylin and eosin (HE) staining. ( Q , R ) Blocking of the C 44 Mab-108 reactivity to OSCC tissue by the CD44 peptide (aa 271–290) containing the C 44 Mab-108 epitope. After antigen retrieval, sections were incubated with C 44 Mab-108 (10 μg/mL) or C 44 Mab-108 (10 μg/mL) plus human CD44 peptide (aa 271–290, 10 μg/mL) followed by treatment with the Envision+ kit. The color was developed using DAB, and sections were counterstained with hematoxylin. Scale bar = 100 µm.

Article Snippet: The formalin-fixed paraffin-embedded (FFPE) OSCC tissue microarray (Product Code: OR601c, US Biomax Inc., Rockville, MD, USA) and the esophageal tissue microarray (Product Code: BC02011, US Biomax Inc.) were deparaffinized in xylene (Sigma-Aldrich Corp.) and rehydrated.

Techniques: Immunohistochemical staining, Incubation, Staining, Blocking Assay