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Becton Dickinson fc-blocking buffer
Fc Blocking Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fc-blocking+buffer/fc+blocking+buffer/pmc05172358-135-7-11
Average 90 stars, based on 1 article reviews
fc-blocking buffer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

other:

Article Title: MS-20 enhances the gut microbiota-associated antitumor effects of anti-PD1 antibody.
Article Snippet: After centrifugation at 300 × g for 5 min, the cells were washed with staining buffer and incubated with Fc-blocking buffer (BD 553,142) to prevent nonspecific binding for 10 min on ice.

Article Title: MS-20 enhances the gut microbiota-associated antitumor effects of anti-PD1 antibody
Article Snippet: After centrifugation at 300 × g for 5 min, the cells were washed with staining buffer and incubated with Fc-blocking buffer (BD 553,142) to prevent nonspecific binding for 10 min on ice.

Incubation:

Article Title: Interleukin-10 disrupts liver repair in acetaminophen-induced acute liver failure.
Article Snippet: .. The cells were then incubated with Fc blocking buffer (BD Biosciences; diluted 1:20) for 10 minutes at 4 °C, frontiersin.org rinsed, and then pelleted by centrifugation at 300 g for 5 minutes. ..

Article Title: Suppression of heterotopic ossification in fibrodysplasia ossificans progressiva using AAV gene delivery.
Article Snippet: Collected splenocytes and bone marrow cells were washed with 1× phosphate-buffered saline (PBS) and then incubated with RBC lysis buffer (BioLegend, #420301) for 2–5min at room temperature to eliminate red blood cells (RBC). .. Further, cells werewashed twice with cold fluorescence-activated cell sorting (FACS) buffer and filtered through a sterile 40μm cell strainer before resuspending into FACS buffer and then incubated with Fc blocking buffer (BD Biosciences, #564765) for 15min at 4 °C. .. After Fc receptor blocking, cells were treated with fluorochrome-labeled antibody cocktail including anti-mouse/human CD11b APC (1:100, BioLegend, #101212), antimouse CD45R (B220) PerCP Cy 5.5 (1:100, Tonbo, #65-0452), antimouse CD3 FITC (1:100, BioLegend, #100203), anti-Mouse CD11c Brilliant Violet 510 (1:100, BioLegend, #117337), anti-mouse Ly6G PECy7 (1:100, BioLegend, #127618) and anti-mouse Ly6C FITC (1:100, BioLegend, #128006) in cold FACS buffer.

Article Title: Differential Sensitivity of Kupffer Cells and Hepatic Monocyte-Derived Macrophages to Bacterial Lipopolysaccharide.
Article Snippet: .. The cells were then incubated with Fc blocking buffer (BD Biosciences; diluted 1:20) for 10 mins at 4°C. ..

Article Title: Interleukin-10 disrupts liver repair in acetaminophen-induced acute liver failure
Article Snippet: .. The cells were then incubated with Fc blocking buffer (BD Biosciences; diluted 1:20) for 10 minutes at 4 °C, rinsed, and then pelleted by centrifugation at 300 g for 5 minutes. ..

Article Title: Augmentation of a neuroprotective myeloid state by hematopoietic cell transplantation
Article Snippet: .. The pellet was resuspended in 100μl FC block buffer (BD Biosciences, 553141, 1:50 in FACS buffer) and 1ul recombinant RNase inhibitor (Takara, 2313B) incubated on ice for 5min. .. FACS buffer consisted of PBS (Gibco, 10010049) containing 1% BSA (Thermo Fisher, BP9700100)).

Blocking Assay:

Article Title: Interleukin-10 disrupts liver repair in acetaminophen-induced acute liver failure.
Article Snippet: .. The cells were then incubated with Fc blocking buffer (BD Biosciences; diluted 1:20) for 10 minutes at 4 °C, frontiersin.org rinsed, and then pelleted by centrifugation at 300 g for 5 minutes. ..

Article Title: Suppression of heterotopic ossification in fibrodysplasia ossificans progressiva using AAV gene delivery.
Article Snippet: Collected splenocytes and bone marrow cells were washed with 1× phosphate-buffered saline (PBS) and then incubated with RBC lysis buffer (BioLegend, #420301) for 2–5min at room temperature to eliminate red blood cells (RBC). .. Further, cells werewashed twice with cold fluorescence-activated cell sorting (FACS) buffer and filtered through a sterile 40μm cell strainer before resuspending into FACS buffer and then incubated with Fc blocking buffer (BD Biosciences, #564765) for 15min at 4 °C. .. After Fc receptor blocking, cells were treated with fluorochrome-labeled antibody cocktail including anti-mouse/human CD11b APC (1:100, BioLegend, #101212), antimouse CD45R (B220) PerCP Cy 5.5 (1:100, Tonbo, #65-0452), antimouse CD3 FITC (1:100, BioLegend, #100203), anti-Mouse CD11c Brilliant Violet 510 (1:100, BioLegend, #117337), anti-mouse Ly6G PECy7 (1:100, BioLegend, #127618) and anti-mouse Ly6C FITC (1:100, BioLegend, #128006) in cold FACS buffer.

Article Title: Differential Sensitivity of Kupffer Cells and Hepatic Monocyte-Derived Macrophages to Bacterial Lipopolysaccharide.
Article Snippet: .. The cells were then incubated with Fc blocking buffer (BD Biosciences; diluted 1:20) for 10 mins at 4°C. ..

Article Title: Interleukin-10 disrupts liver repair in acetaminophen-induced acute liver failure
Article Snippet: .. The cells were then incubated with Fc blocking buffer (BD Biosciences; diluted 1:20) for 10 minutes at 4 °C, rinsed, and then pelleted by centrifugation at 300 g for 5 minutes. ..

Article Title: Augmentation of a neuroprotective myeloid state by hematopoietic cell transplantation
Article Snippet: .. The pellet was resuspended in 100μl FC block buffer (BD Biosciences, 553141, 1:50 in FACS buffer) and 1ul recombinant RNase inhibitor (Takara, 2313B) incubated on ice for 5min. .. FACS buffer consisted of PBS (Gibco, 10010049) containing 1% BSA (Thermo Fisher, BP9700100)).

Centrifugation:

Article Title: Interleukin-10 disrupts liver repair in acetaminophen-induced acute liver failure.
Article Snippet: .. The cells were then incubated with Fc blocking buffer (BD Biosciences; diluted 1:20) for 10 minutes at 4 °C, frontiersin.org rinsed, and then pelleted by centrifugation at 300 g for 5 minutes. ..

Article Title: Interleukin-10 disrupts liver repair in acetaminophen-induced acute liver failure
Article Snippet: .. The cells were then incubated with Fc blocking buffer (BD Biosciences; diluted 1:20) for 10 minutes at 4 °C, rinsed, and then pelleted by centrifugation at 300 g for 5 minutes. ..

Fluorescence:

Article Title: Suppression of heterotopic ossification in fibrodysplasia ossificans progressiva using AAV gene delivery.
Article Snippet: Collected splenocytes and bone marrow cells were washed with 1× phosphate-buffered saline (PBS) and then incubated with RBC lysis buffer (BioLegend, #420301) for 2–5min at room temperature to eliminate red blood cells (RBC). .. Further, cells werewashed twice with cold fluorescence-activated cell sorting (FACS) buffer and filtered through a sterile 40μm cell strainer before resuspending into FACS buffer and then incubated with Fc blocking buffer (BD Biosciences, #564765) for 15min at 4 °C. .. After Fc receptor blocking, cells were treated with fluorochrome-labeled antibody cocktail including anti-mouse/human CD11b APC (1:100, BioLegend, #101212), antimouse CD45R (B220) PerCP Cy 5.5 (1:100, Tonbo, #65-0452), antimouse CD3 FITC (1:100, BioLegend, #100203), anti-Mouse CD11c Brilliant Violet 510 (1:100, BioLegend, #117337), anti-mouse Ly6G PECy7 (1:100, BioLegend, #127618) and anti-mouse Ly6C FITC (1:100, BioLegend, #128006) in cold FACS buffer.

FACS:

Article Title: Suppression of heterotopic ossification in fibrodysplasia ossificans progressiva using AAV gene delivery.
Article Snippet: Collected splenocytes and bone marrow cells were washed with 1× phosphate-buffered saline (PBS) and then incubated with RBC lysis buffer (BioLegend, #420301) for 2–5min at room temperature to eliminate red blood cells (RBC). .. Further, cells werewashed twice with cold fluorescence-activated cell sorting (FACS) buffer and filtered through a sterile 40μm cell strainer before resuspending into FACS buffer and then incubated with Fc blocking buffer (BD Biosciences, #564765) for 15min at 4 °C. .. After Fc receptor blocking, cells were treated with fluorochrome-labeled antibody cocktail including anti-mouse/human CD11b APC (1:100, BioLegend, #101212), antimouse CD45R (B220) PerCP Cy 5.5 (1:100, Tonbo, #65-0452), antimouse CD3 FITC (1:100, BioLegend, #100203), anti-Mouse CD11c Brilliant Violet 510 (1:100, BioLegend, #117337), anti-mouse Ly6G PECy7 (1:100, BioLegend, #127618) and anti-mouse Ly6C FITC (1:100, BioLegend, #128006) in cold FACS buffer.

Article Title: Augmentation of a neuroprotective myeloid state by hematopoietic cell transplantation
Article Snippet: .. The pellet was resuspended in 100μl FC block buffer (BD Biosciences, 553141, 1:50 in FACS buffer) and 1ul recombinant RNase inhibitor (Takara, 2313B) incubated on ice for 5min. .. FACS buffer consisted of PBS (Gibco, 10010049) containing 1% BSA (Thermo Fisher, BP9700100)).

Sterility:

Article Title: Suppression of heterotopic ossification in fibrodysplasia ossificans progressiva using AAV gene delivery.
Article Snippet: Collected splenocytes and bone marrow cells were washed with 1× phosphate-buffered saline (PBS) and then incubated with RBC lysis buffer (BioLegend, #420301) for 2–5min at room temperature to eliminate red blood cells (RBC). .. Further, cells werewashed twice with cold fluorescence-activated cell sorting (FACS) buffer and filtered through a sterile 40μm cell strainer before resuspending into FACS buffer and then incubated with Fc blocking buffer (BD Biosciences, #564765) for 15min at 4 °C. .. After Fc receptor blocking, cells were treated with fluorochrome-labeled antibody cocktail including anti-mouse/human CD11b APC (1:100, BioLegend, #101212), antimouse CD45R (B220) PerCP Cy 5.5 (1:100, Tonbo, #65-0452), antimouse CD3 FITC (1:100, BioLegend, #100203), anti-Mouse CD11c Brilliant Violet 510 (1:100, BioLegend, #117337), anti-mouse Ly6G PECy7 (1:100, BioLegend, #127618) and anti-mouse Ly6C FITC (1:100, BioLegend, #128006) in cold FACS buffer.

Recombinant:

Article Title: Augmentation of a neuroprotective myeloid state by hematopoietic cell transplantation
Article Snippet: .. The pellet was resuspended in 100μl FC block buffer (BD Biosciences, 553141, 1:50 in FACS buffer) and 1ul recombinant RNase inhibitor (Takara, 2313B) incubated on ice for 5min. .. FACS buffer consisted of PBS (Gibco, 10010049) containing 1% BSA (Thermo Fisher, BP9700100)).



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