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fc receptor blocking solution  (Cell Signaling Technology Inc)


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Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc fc receptor blocking solution
    Fc Receptor Blocking Solution, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+blocking+solution/CD16%2FCD32+(2%2E4G2)+Rat+mAb/pmc12173058-161-28-33
    Average 94 stars, based on 13 article reviews
    fc receptor blocking solution - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Adeno-associated virus–based gene therapy treats inflammatory kidney disease in mice
    Article Snippet: .. Cryosections were incubated overnight at 4°C with the following primary antibodies: chicken anti-GFP, 1:500 (Thermo Fisher Scientific, A10262); rat anti-CD31, 1:200 (BD Pharmingen, 550274); rat anti-CD16/CD32, 1:200 (Cell Signaling Technology, 88280S); rabbit anti-WT1, 1:200 (Abcam, ab89901); rabbit anti-PDGFR, 1:200 (Abcam, ab32570); mouse anti-goat/sheep IgG-Biotin (Fc-specific), 1:1,000 (Sigma-Aldrich, B3148); Alexa Fluor 555 donkey anti-sheep IgG, 1:1,000 (Invitrogen, A21436); Alexa Fluor 555 donkey anti-mouse IgG, 1:1,000 (Invitrogen, A31570); Mouse anti-RECA-1, 1:200 (Novus Biologicals, NB600-1388); rabbit anti-C1q, 1:200 (Abcam, ab182451); rat anti-C3, 1:200 (Novus Biologicals, NB200-540SS). .. Alexa Fluor 555 conjugated Streptavidin and Alexa Fluor 488-, 594-, or 647-labeled secondary antibodies (Invitrogen) were incubated at room temperature for 1 hour.

    Article Title: Adeno-associated virus–based gene therapy treats inflammatory kidney disease in mice
    Article Snippet: .. Cryosections were incubated overnight at 4°C with the following primary antibodies: chicken antiGFP, 1:500 (Thermo Fisher Scientific, A10262); rat anti-CD31, 1:200 (BD Pharmingen, 550274); rat anti-CD16/CD32, 1:200 (Cell Signaling Technology, 88280S); rabbit anti-WT1, 1:200 (Abcam, ab89901); rabbit anti-PDGFR, 1:200 (Abcam, ab32570); mouse anti-goat/sheep IgG-Biotin (Fc-specific), 1:1,000 (Sigma-Aldrich, B3148); Alexa Fluor 555 donkey anti-sheep IgG, 1:1,000 (Invitrogen, A21436); Alexa Fluor 555 donkey anti-mouse IgG, 1:1,000 (Invitrogen, A31570); Mouse anti-RECA-1, 1:200 (Novus Biologicals, NB600-1388); rabbit antiC1q, 1:200 (Abcam, ab182451); rat anti-C3, 1:200 (Novus Biologicals, NB200-540SS). .. Alexa Fluor 555 conjugated Streptavidin and Alexa Fluor 488-, 594-, or 647-labeled secondary antibodies (Invitrogen) were incubated at room temperature for 1 hour.

    In Vitro:

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming
    Article Snippet: .. Cells were collected in vitro , or single-cell suspensions were prepared from lymph nodes or tumor tissues via enzymatic digestion in vivo , followed by the addition of Fc receptor blocking solution (88280S, Cell Signaling Technology). ..

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming.
    Article Snippet: .. Cells were collected in vitro, or single-cell suspensions were prepared from lymph nodes or tumor tissues via enzymatic digestion in vivo, followed by the addition of Fc receptor blocking solution (88280S, Cell Signaling Technology). ..

    Single Cell:

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming
    Article Snippet: .. Cells were collected in vitro , or single-cell suspensions were prepared from lymph nodes or tumor tissues via enzymatic digestion in vivo , followed by the addition of Fc receptor blocking solution (88280S, Cell Signaling Technology). ..

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming.
    Article Snippet: .. Cells were collected in vitro, or single-cell suspensions were prepared from lymph nodes or tumor tissues via enzymatic digestion in vivo, followed by the addition of Fc receptor blocking solution (88280S, Cell Signaling Technology). ..

    In Vivo:

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming
    Article Snippet: .. Cells were collected in vitro , or single-cell suspensions were prepared from lymph nodes or tumor tissues via enzymatic digestion in vivo , followed by the addition of Fc receptor blocking solution (88280S, Cell Signaling Technology). ..

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming.
    Article Snippet: .. Cells were collected in vitro, or single-cell suspensions were prepared from lymph nodes or tumor tissues via enzymatic digestion in vivo, followed by the addition of Fc receptor blocking solution (88280S, Cell Signaling Technology). ..

    Blocking Assay:

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming
    Article Snippet: .. Cells were collected in vitro , or single-cell suspensions were prepared from lymph nodes or tumor tissues via enzymatic digestion in vivo , followed by the addition of Fc receptor blocking solution (88280S, Cell Signaling Technology). ..

    Article Title: HOXA10 drives immune evasion in early lung adenocarcinoma by recruiting immunosuppressive macrophages via NF-κB/CCL2 signaling
    Article Snippet: Fresh tumor tissues were excised and enzymatically digested with collagenase IV & I (Gibco) and DNase I (Roche) for 40 min, then filtered through 70-μm cell strainers to obtain single-cell suspensions. .. After blocking non-specific antibody binding with CD16/CD32 antibody (CST, #88,280), surface staining was performed with relative antibodies listed in Supplementary methods. .. After fixation and permeabilization using a Fixation/Permeabilization kit (Invitrogen, GAS003), intracellular IFN-γ was stained with anti-IFN-γ antibody (BD, #562,020).

    Article Title: Knockout of cyclin dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice
    Article Snippet: .. To reduce potential nonspecific antibody staining, Mouse Fc Block (CD16/CD32) (Cell Signaling, #88280) was used as described by the manufacturer. ..

    Article Title: Knockout of cyclin-dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice
    Article Snippet: .. To reduce potential nonspecific antibody staining, Mouse Fc Block (CD16/CD32) (Cell Signaling, #88280) was used as described by the manufacturer. ..

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming.
    Article Snippet: .. Cells were collected in vitro, or single-cell suspensions were prepared from lymph nodes or tumor tissues via enzymatic digestion in vivo, followed by the addition of Fc receptor blocking solution (88280S, Cell Signaling Technology). ..

    Binding Assay:

    Article Title: HOXA10 drives immune evasion in early lung adenocarcinoma by recruiting immunosuppressive macrophages via NF-κB/CCL2 signaling
    Article Snippet: Fresh tumor tissues were excised and enzymatically digested with collagenase IV & I (Gibco) and DNase I (Roche) for 40 min, then filtered through 70-μm cell strainers to obtain single-cell suspensions. .. After blocking non-specific antibody binding with CD16/CD32 antibody (CST, #88,280), surface staining was performed with relative antibodies listed in Supplementary methods. .. After fixation and permeabilization using a Fixation/Permeabilization kit (Invitrogen, GAS003), intracellular IFN-γ was stained with anti-IFN-γ antibody (BD, #562,020).

    Staining:

    Article Title: HOXA10 drives immune evasion in early lung adenocarcinoma by recruiting immunosuppressive macrophages via NF-κB/CCL2 signaling
    Article Snippet: Fresh tumor tissues were excised and enzymatically digested with collagenase IV & I (Gibco) and DNase I (Roche) for 40 min, then filtered through 70-μm cell strainers to obtain single-cell suspensions. .. After blocking non-specific antibody binding with CD16/CD32 antibody (CST, #88,280), surface staining was performed with relative antibodies listed in Supplementary methods. .. After fixation and permeabilization using a Fixation/Permeabilization kit (Invitrogen, GAS003), intracellular IFN-γ was stained with anti-IFN-γ antibody (BD, #562,020).

    Article Title: Knockout of cyclin dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice
    Article Snippet: .. To reduce potential nonspecific antibody staining, Mouse Fc Block (CD16/CD32) (Cell Signaling, #88280) was used as described by the manufacturer. ..

    Article Title: Knockout of cyclin-dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice
    Article Snippet: .. To reduce potential nonspecific antibody staining, Mouse Fc Block (CD16/CD32) (Cell Signaling, #88280) was used as described by the manufacturer. ..



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