anti-francisella tularensis lps fb11 clone (Thermo Fisher)
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Anti Francisella Tularensis Lps Fb11 Clone, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fb11/anti++f++tularensis+lipopolysaccharide++lps++antibody++clone+fb11+/pmc10141597-237-30-36
Average 90 stars, based on 1 article reviews
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1) Product Images from "Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids"
Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
Journal: ACS Central Science
doi: 10.1021/acscentsci.2c01473
Figure Legend Snippet: Experimental framework for profiling GC-like B cell responses to glycoconjugate vaccine candidates. (a) Schematic of strategies for preparing designer glycoconjugate vaccine candidates (left) and evaluating their immunogenicity in mice and in synthetic immune organoids (right). Glycoengineered bacteria enable the assembly of a single O-PS repeat unit (a tetrasaccharide structure in the case of F. tularensis Schu S4) on a undecaprenol lipid carrier in the cytoplasmic membrane, which is subsequently flipped into the periplasm and polymerized to form variable-length O-PS antigens by the endogenous Wzx flippase and Wzy O-antigen polymerase Wzy, respectively. In the presence of C. jejuni PglB ( Cj PglB), the lipid-linked O-PS is site-specifically transferred to asparagine residues in recombinant carrier proteins bearing a DQNAT motif. Schematic created with BioRender.com . (b) Immunoblot analysis of purified carrier proteins derived from E. coli CLM24 cells carrying a plasmid encoding either MBP 4xDQNAT or CRM 197 4xDQNAT along with plasmid pGAB2 encoding the Ft O-PS biosynthetic pathway and with (+) or without (−) plasmid pMAF10 encoding Cj PglB as indicated. Blots were probed with anti-FLAG antibody to detect acceptor proteins (green signal) and FB11 antibody to detect Ft O-PS antigens (red signal). Images depict an overlay of anti-FLAG and FB11 blots. Arrows denote aglycosylated (agly) and multiply glycosylated (gly) forms of MBP 4xDQNAT and CRM 197 4xDQNAT . Molecular weight ( M W ) markers are indicated on the left. Results are representative of three biological replicates.
Techniques Used: Immunopeptidomics, Bacteria, Membrane, Recombinant, Western Blot, Purification, Derivative Assay, Plasmid Preparation, Molecular Weight
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Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Infection:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. 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Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Immunohistochemistry:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Incubation:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Conjugation Assay:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Flow Cytometry:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Control:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Expressing:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Isolation:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Selection:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, RNA Sequencing:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Gene Expression:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Fluorescence:Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria Article Snippet: Staining was performed using an Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids Article Snippet: Antimouse antiboides used for confocal imaging included the following: Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay. Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, |
![Antigenic change in lipopolysaccharide (LPS) of KU-1 variants passaged on Eugon chocolate agar in Western blots. Purified LPSs of KU-1 original and the variants passaged on Eugon chocolate agar 10, 20, and 80 times (EP10, EP20, and EP80, respectively) and LVS (lanes 1, 2, 3, 4, and 5, respectively) were detected using MAbs M15C6, M13A13, and <t>FB11</t> [sheets ( A ), ( B ), and ( C ), respectively]. M: molecular weight markers (Bio-Rad) indicate 75, 50, 37, 25, 20, 15, and 10 (arrow) kDa from the top.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0542/pmc07760542/pmc07760542__microorganisms-08-01881-g009.jpg)