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anti-francisella tularensis lps fb11 clone  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti-francisella tularensis lps fb11 clone
    Experimental framework for profiling GC-like B cell responses to glycoconjugate vaccine candidates. (a) Schematic of strategies for preparing designer glycoconjugate vaccine candidates (left) and evaluating their immunogenicity in mice and in synthetic immune organoids (right). Glycoengineered bacteria enable the assembly of a single O-PS repeat unit (a tetrasaccharide structure in the case of F. <t>tularensis</t> Schu S4) on a undecaprenol lipid carrier in the cytoplasmic membrane, which is subsequently flipped into the periplasm and polymerized to form variable-length O-PS antigens by the endogenous Wzx flippase and Wzy O-antigen polymerase Wzy, respectively. In the presence of C. jejuni PglB ( Cj PglB), the lipid-linked O-PS is site-specifically transferred to asparagine residues in recombinant carrier proteins bearing a DQNAT motif. Schematic created with BioRender.com . (b) Immunoblot analysis of purified carrier proteins derived from E. coli CLM24 cells carrying a plasmid encoding either MBP 4xDQNAT or CRM 197 4xDQNAT along with plasmid pGAB2 encoding the Ft O-PS biosynthetic pathway and with (+) or without (−) plasmid pMAF10 encoding Cj PglB as indicated. Blots were probed with anti-FLAG antibody to detect acceptor proteins (green signal) and <t>FB11</t> antibody to detect Ft O-PS antigens (red signal). Images depict an overlay of anti-FLAG and FB11 blots. Arrows denote aglycosylated (agly) and multiply glycosylated (gly) forms of MBP 4xDQNAT and CRM 197 4xDQNAT . Molecular weight ( M W ) markers are indicated on the left. Results are representative of three biological replicates.
    Anti Francisella Tularensis Lps Fb11 Clone, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fb11/anti++f++tularensis+lipopolysaccharide++lps++antibody++clone+fb11+/pmc10141597-237-30-36
    Average 90 stars, based on 1 article reviews
    anti-francisella tularensis lps fb11 clone - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids"

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids

    Journal: ACS Central Science

    doi: 10.1021/acscentsci.2c01473

    Experimental framework for profiling GC-like B cell responses to glycoconjugate vaccine candidates. (a) Schematic of strategies for preparing designer glycoconjugate vaccine candidates (left) and evaluating their immunogenicity in mice and in synthetic immune organoids (right). Glycoengineered bacteria enable the assembly of a single O-PS repeat unit (a tetrasaccharide structure in the case of F. tularensis Schu S4) on a undecaprenol lipid carrier in the cytoplasmic membrane, which is subsequently flipped into the periplasm and polymerized to form variable-length O-PS antigens by the endogenous Wzx flippase and Wzy O-antigen polymerase Wzy, respectively. In the presence of C. jejuni PglB ( Cj PglB), the lipid-linked O-PS is site-specifically transferred to asparagine residues in recombinant carrier proteins bearing a DQNAT motif. Schematic created with BioRender.com . (b) Immunoblot analysis of purified carrier proteins derived from E. coli CLM24 cells carrying a plasmid encoding either MBP 4xDQNAT or CRM 197 4xDQNAT along with plasmid pGAB2 encoding the Ft O-PS biosynthetic pathway and with (+) or without (−) plasmid pMAF10 encoding Cj PglB as indicated. Blots were probed with anti-FLAG antibody to detect acceptor proteins (green signal) and FB11 antibody to detect Ft O-PS antigens (red signal). Images depict an overlay of anti-FLAG and FB11 blots. Arrows denote aglycosylated (agly) and multiply glycosylated (gly) forms of MBP 4xDQNAT and CRM 197 4xDQNAT . Molecular weight ( M W ) markers are indicated on the left. Results are representative of three biological replicates.
    Figure Legend Snippet: Experimental framework for profiling GC-like B cell responses to glycoconjugate vaccine candidates. (a) Schematic of strategies for preparing designer glycoconjugate vaccine candidates (left) and evaluating their immunogenicity in mice and in synthetic immune organoids (right). Glycoengineered bacteria enable the assembly of a single O-PS repeat unit (a tetrasaccharide structure in the case of F. tularensis Schu S4) on a undecaprenol lipid carrier in the cytoplasmic membrane, which is subsequently flipped into the periplasm and polymerized to form variable-length O-PS antigens by the endogenous Wzx flippase and Wzy O-antigen polymerase Wzy, respectively. In the presence of C. jejuni PglB ( Cj PglB), the lipid-linked O-PS is site-specifically transferred to asparagine residues in recombinant carrier proteins bearing a DQNAT motif. Schematic created with BioRender.com . (b) Immunoblot analysis of purified carrier proteins derived from E. coli CLM24 cells carrying a plasmid encoding either MBP 4xDQNAT or CRM 197 4xDQNAT along with plasmid pGAB2 encoding the Ft O-PS biosynthetic pathway and with (+) or without (−) plasmid pMAF10 encoding Cj PglB as indicated. Blots were probed with anti-FLAG antibody to detect acceptor proteins (green signal) and FB11 antibody to detect Ft O-PS antigens (red signal). Images depict an overlay of anti-FLAG and FB11 blots. Arrows denote aglycosylated (agly) and multiply glycosylated (gly) forms of MBP 4xDQNAT and CRM 197 4xDQNAT . Molecular weight ( M W ) markers are indicated on the left. Results are representative of three biological replicates.

    Techniques Used: Immunopeptidomics, Bacteria, Membrane, Recombinant, Western Blot, Purification, Derivative Assay, Plasmid Preparation, Molecular Weight

    Related Articles

    Imaging:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Immunopeptidomics:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Bacteria:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Membrane:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Recombinant:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Western Blot:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Purification:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Derivative Assay:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Plasmid Preparation:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Molecular Weight:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Infection:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    H&E Stain:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Immunohistochemistry:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Incubation:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Conjugation Assay:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Flow Cytometry:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Control:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Expressing:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Isolation:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Selection:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    RNA Sequencing:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Gene Expression:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.

    Fluorescence:

    Article Title: Francisella tularensis PCR detection in Cape hares ( Lepus capensis ) and wild rabbits ( Oryctolagus cuniculus ) in Algeria
    Article Snippet: Staining was performed using an F. tularensis LPS-directed mouse monoclonal antibody (FB11, used at 1:1000, ThermoFisher Scientific, Rockford USA) applied for one hour at room temperature.

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Article Title: CD200R deletion promotes a neutrophil niche for Francisella tularensis and increases infectious burden and mortality
    Article Snippet: Cells were then washed in Permeabilisation buffer (eBioscience), centrifuged and incubated with anti- F. tularensis lipopolysaccharide (LPS) antibody (clone FB11, Thermo #MA1-21690) labelled with APC Conjugation Kit (Abcam) in permeabilisation buffer (eBioscience) for an additional 30 min at 4 °C.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay.
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www. thermofisher.com) as the primary antibody at 1:1,000 dilution.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: A blocking step for endogenous peroxidase was performed with 3% aqueous solution of hydrogen peroxide for 10 min. A specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, Francisella tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com), was applied as the primary antibody at a 1:1,000 dilution for 45 min at room temperature.

    Article Title: Pathologic and Immunohistochemical Evidence of Possible Francisellaceae among Aborted Ovine Fetuses, Uruguay
    Article Snippet: We applied a specific mouse monoclonal IgG3 raised against F. tularensis lipopolysaccharide, F. tularensis LPS Monoclonal Antibody (T14) (Thermo Fisher Scientific, https://www.thermofisher.com ) as the primary antibody at 1:1,000 dilution.



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    Antigenic change in lipopolysaccharide (LPS) of KU-1 variants passaged on Eugon chocolate agar in Western blots. Purified LPSs of KU-1 original and the variants passaged on Eugon chocolate agar 10, 20, and 80 times (EP10, EP20, and EP80, respectively) and LVS (lanes 1, 2, 3, 4, and 5, respectively) were detected using MAbs M15C6, M13A13, and <t>FB11</t> [sheets ( A ), ( B ), and ( C ), respectively]. M: molecular weight markers (Bio-Rad) indicate 75, 50, 37, 25, 20, 15, and 10 (arrow) kDa from the top.
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    Image Search Results


    Experimental framework for profiling GC-like B cell responses to glycoconjugate vaccine candidates. (a) Schematic of strategies for preparing designer glycoconjugate vaccine candidates (left) and evaluating their immunogenicity in mice and in synthetic immune organoids (right). Glycoengineered bacteria enable the assembly of a single O-PS repeat unit (a tetrasaccharide structure in the case of F. tularensis Schu S4) on a undecaprenol lipid carrier in the cytoplasmic membrane, which is subsequently flipped into the periplasm and polymerized to form variable-length O-PS antigens by the endogenous Wzx flippase and Wzy O-antigen polymerase Wzy, respectively. In the presence of C. jejuni PglB ( Cj PglB), the lipid-linked O-PS is site-specifically transferred to asparagine residues in recombinant carrier proteins bearing a DQNAT motif. Schematic created with BioRender.com . (b) Immunoblot analysis of purified carrier proteins derived from E. coli CLM24 cells carrying a plasmid encoding either MBP 4xDQNAT or CRM 197 4xDQNAT along with plasmid pGAB2 encoding the Ft O-PS biosynthetic pathway and with (+) or without (−) plasmid pMAF10 encoding Cj PglB as indicated. Blots were probed with anti-FLAG antibody to detect acceptor proteins (green signal) and FB11 antibody to detect Ft O-PS antigens (red signal). Images depict an overlay of anti-FLAG and FB11 blots. Arrows denote aglycosylated (agly) and multiply glycosylated (gly) forms of MBP 4xDQNAT and CRM 197 4xDQNAT . Molecular weight ( M W ) markers are indicated on the left. Results are representative of three biological replicates.

    Journal: ACS Central Science

    Article Title: Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids

    doi: 10.1021/acscentsci.2c01473

    Figure Lengend Snippet: Experimental framework for profiling GC-like B cell responses to glycoconjugate vaccine candidates. (a) Schematic of strategies for preparing designer glycoconjugate vaccine candidates (left) and evaluating their immunogenicity in mice and in synthetic immune organoids (right). Glycoengineered bacteria enable the assembly of a single O-PS repeat unit (a tetrasaccharide structure in the case of F. tularensis Schu S4) on a undecaprenol lipid carrier in the cytoplasmic membrane, which is subsequently flipped into the periplasm and polymerized to form variable-length O-PS antigens by the endogenous Wzx flippase and Wzy O-antigen polymerase Wzy, respectively. In the presence of C. jejuni PglB ( Cj PglB), the lipid-linked O-PS is site-specifically transferred to asparagine residues in recombinant carrier proteins bearing a DQNAT motif. Schematic created with BioRender.com . (b) Immunoblot analysis of purified carrier proteins derived from E. coli CLM24 cells carrying a plasmid encoding either MBP 4xDQNAT or CRM 197 4xDQNAT along with plasmid pGAB2 encoding the Ft O-PS biosynthetic pathway and with (+) or without (−) plasmid pMAF10 encoding Cj PglB as indicated. Blots were probed with anti-FLAG antibody to detect acceptor proteins (green signal) and FB11 antibody to detect Ft O-PS antigens (red signal). Images depict an overlay of anti-FLAG and FB11 blots. Arrows denote aglycosylated (agly) and multiply glycosylated (gly) forms of MBP 4xDQNAT and CRM 197 4xDQNAT . Molecular weight ( M W ) markers are indicated on the left. Results are representative of three biological replicates.

    Article Snippet: Antimouse antiboides used for confocal imaging included the following: anti-CD19 polyclonal (ThermoFisher Cat # PA5-27442); anti-IgM II/41 clone (ThermoFisher Cat # 14-5790-82); antidiphtheria toxin polyclonal (Abcam Cat # ab151222); and anti- Francisella tularensis LPS FB11 clone (ThermoFisher Cat # MA1-21690).

    Techniques: Immunopeptidomics, Bacteria, Membrane, Recombinant, Western Blot, Purification, Derivative Assay, Plasmid Preparation, Molecular Weight

    Antigenic change in lipopolysaccharide (LPS) of KU-1 variants passaged on Eugon chocolate agar in Western blots. Purified LPSs of KU-1 original and the variants passaged on Eugon chocolate agar 10, 20, and 80 times (EP10, EP20, and EP80, respectively) and LVS (lanes 1, 2, 3, 4, and 5, respectively) were detected using MAbs M15C6, M13A13, and FB11 [sheets ( A ), ( B ), and ( C ), respectively]. M: molecular weight markers (Bio-Rad) indicate 75, 50, 37, 25, 20, 15, and 10 (arrow) kDa from the top.

    Journal: Microorganisms

    Article Title: Virulence of Francisella tularensis Subspecies holarctica Biovar japonica and Phenotypic Change during Serial Passages on Artificial Media

    doi: 10.3390/microorganisms8121881

    Figure Lengend Snippet: Antigenic change in lipopolysaccharide (LPS) of KU-1 variants passaged on Eugon chocolate agar in Western blots. Purified LPSs of KU-1 original and the variants passaged on Eugon chocolate agar 10, 20, and 80 times (EP10, EP20, and EP80, respectively) and LVS (lanes 1, 2, 3, 4, and 5, respectively) were detected using MAbs M15C6, M13A13, and FB11 [sheets ( A ), ( B ), and ( C ), respectively]. M: molecular weight markers (Bio-Rad) indicate 75, 50, 37, 25, 20, 15, and 10 (arrow) kDa from the top.

    Article Snippet: FB11 was purchased from Biodesign International (Saco, ME, USA).

    Techniques: Chocolate, Western Blot, Purification, Molecular Weight