4 µl fastpfu buffer (5×) (Beijing TransGen Biotech)

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Beijing TransGen Biotech 4 µl fastpfu buffer (5×)
4 µl Fastpfu Buffer (5×), supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fastpfu+buffer/1+%C3%97+fastpfu+buffer/pm39597525-75-8-33
Average 90 stars, based on 1 article reviews

4 µl fastpfu buffer (5×) - by Bioz Stars, 2026-10

90/100 stars

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Amplification:

Article Title: Soil Properties Regulate Soil Microbial Communities During Forest Succession in a Karst Region of Southwest China
Article Snippet: .. The bacterial amplification solution consisted of 4 μL FastPfu Buffer (5×), 2 μL dNTPs (2.5 mM), 0.8 μL each of Forward Primer (5 μM) and Reverse Primer (5 μM), 0.4 μL FastPfu Polymerse (Beijing TransGen Biotech Co., Ltd., Beijing, China), 0.2 μL BSA, 10 ng Template DNA, and ddH 2 O to a final volume of 20 μL. ..

Article Title: Soil Properties Regulate Soil Microbial Communities During Forest Succession in a Karst Region of Southwest China.
Article Snippet: .. The bacterial amplification solution consisted of 4 μL FastPfu Buffer (5×), 2 μL dNTPs (2.5 mM), 0.8 μL each of Forward Primer (5 μM) and Reverse Primer (5 μM), 0.4 μL FastPfu Polymerse (Beijing TransGen Biotech Co., Ltd., Beijing, China), 0.2 μL BSA, 10 ng Template DNA, and ddH2O to a final volume of 20 μL. ..

Polymerase Chain Reaction:

Article Title: Variation and Correlation Analysis of Flavour and Bacterial Diversity of Low-Salt Hotpot Sauce during Storage.
Article Snippet: .. The PCR mix consisted of 4 μL of 5× TransStart FastPfu buffer, 2 μL of dNTP solution (2.5 mmol/L), 0.8 μL of upstream primer (5 μmol/L), 0.8 μL of downstream primer (5 μmol/L), 0.4 μL of TransStart FastPfu DNA polymerase (Beijing TransGen Biotechnology Co., Ltd., Beijing, China), and 10 ng of template DNA comprising 20 μL of distilled water. ..

Article Title: Microbiome-Metabolites Analysis Reveals Unhealthy Alterations in the Gut Microbiota but Improved Meat Quality with a High-Rice Diet Challenge in a Small Ruminant Model
Article Snippet: .. PCR was performed in a total of 20 μL volume, containing 1 × FastPfu Buffer, 250 μM dNTP, 0.1 μM each of the primer, 1 U FastPfu Polymerase (Beijing TransGen Biotech, Beijing, China) and 10 ng template DNA. ..

Article Title: Compositional Shifts in the Mycobiota of 'Shine Muscat' Grape ( Vitis labruscana Baily × V. vinifera L.) Bunches During Cold Storage at Different Temperatures.
Article Snippet: .. PCR reactions were conducted in triplicate in a 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase (All PCR reagents were purchased from Beijing TransGen Biotech Co., Ltd., Beijing, China), and 10 ng of template DNA on a GeneAmp 9700 thermocycler (ABI, Foster city, CA, USA) with the following program: 3 min of denaturation at 95 ◦C, 33 cycles of 30 s at 95 ◦C, 30 s for annealing at 55 ◦C, and 45 s for elongation at 72 ◦C, and a final extension at 72 ◦C for 10 min. .. The PCR products were then extracted from a 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), and quantified using QuantiFluorTM-ST (Promega, Madison, WI, USA) according to the manufacturer’s protocol.

Article Title: Fermented Soy and Fish Protein Dietary Sources Shape Ileal and Colonic Microbiota, Improving Nutrient Digestibility and Host Health in a Piglet Model
Article Snippet: .. Polymerase chain reaction (PCR) was conducted in a total volume of 20 μL, including 1 × FastPfu buffer, 250 μM dNTP, 0.2 μM each primer, 1 U FastPfu polymerase (Beijing TransGen Biotech, Beijing, China), and 10 ng template DNA. .. Thermal cycling parameters: initial denaturation at 98°C for 1 min, followed by 30 cycles of 98°C for 10 s, annealing at 50°C for 30 s, elongation at 72°C for 30 s, and a final extension at 72°C for 5 min. PCR products were electrophoresed on 2% agarose gels and purified using a Qiagen gel extraction kit (Qiagen, Hilden, Germany).

Article Title: Identification and Validation of New Stable QTLs for Grain Weight and Size by Multiple Mapping Models in Common Wheat
Article Snippet: .. PCR amplifications were performed in 10-μL volumes that included 0.25 μM of each primer, 0.25 mM dNTPs, 100 ng genomic DNA, 0.5 unit Fastpfu polymerase, and 1 μL of 10 × Fastpfu PCR buffer (Beijing TransGen Biotech, Beijing, China). ..

Article Title: Compositional Shifts in the Mycobiota of ‘Shine Muscat’ Grape ( Vitis labruscana Baily × V. vinifera L.) Bunches During Cold Storage at Different Temperatures
Article Snippet: .. PCR reactions were conducted in triplicate in a 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase (All PCR reagents were purchased from Beijing TransGen Biotech Co., Ltd., Beijing, China), and 10 ng of template DNA on a GeneAmp 9700 thermocycler (ABI, Foster city, CA, USA) with the following program: 3 min of denaturation at 95 °C, 33 cycles of 30 s at 95 °C, 30 s for annealing at 55 °C, and 45 s for elongation at 72 °C, and a final extension at 72 °C for 10 min. .. The PCR products were then extracted from a 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), and quantified using QuantiFluorTM-ST (Promega, Madison, WI, USA) according to the manufacturer’s protocol.

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4 µl fastpfu buffer (5×) (Beijing TransGen Biotech)

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