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TransGen biotech co 5× fastpfu buffer
5× Fastpfu Buffer, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fastpfu+buffer+(5/5%C3%97+fastpfu+buffer/pmc09019705-31-0-4
Average 90 stars, based on 1 article reviews
5× fastpfu buffer - by Bioz Stars, 2026-11
90/100 stars

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Polymerase Chain Reaction:

Article Title: Arbuscular mycorrhizal fungal communities in soils where astragalus had grown for 2 years were similar to those in the abandoned farmland
Article Snippet: .. PCR reaction system consisted of 20 μL, including 5 × FastPfu Buffer 4 μL, 2.5 mM dNTPs 2 μL, 5 μM forward primer 0.8 μL, 5 μM reverse primer 0.8 μL, FastPfu DNA polymerase (TransGen Biotech) 0.4 μL, bovine serum albumin 0.2 μL, template DNA 10 ng, and dd H 2 O to reach a final volume of 20 μL. ..

Article Title: Waste seaweed compost and rhizosphere bacteria Pseudomonas koreensis promote tomato seedlings growth by benefiting properties, enzyme activities and rhizosphere bacterial community in coastal saline soil of Yellow River Delta, China.
Article Snippet: This study investigated the effects of waste seaweed compost and rhizosphere bacteria Pseudomonas koreensis HCH2-3 on the tomato seedlings growth in coastal saline soils and chemical properties, enzyme activities, microbial communities of rhizosphere soil.. Microcosmic experiment showed that the seaweed compost and rhizosphere bacteria (SC + HCH2-3) significantly alleviated the negative effects of salinity on the growth of tomato seedlings.. SC + HCH2-3 amendment significantly increased the plant height and root fresh biomass of tomato seedling by 105.59% and 55.60% in the coastal saline soils, respectively.

Article Title: Contrasting Effects of Grazing in Shaping the Seasonal Trajectory of Foliar Fungal Endophyte Communities on Two Semiarid Grassland Species
Article Snippet: .. PCRs for each sample were carried out in triplicate 20-μL reactions with 0.8 μL of each primer at 5 μmol·L −1 , 10 ng template DNA, 2 μL dNTPs at 2.5 mmol·L −1 , 0.4 μL FastPfu Polymerase (TransGen AP221-02; TransStart FastPfu DNA Polymerase; TransGen Biotech, Beijing, China), 4 μL 5× FastPfu buffer, 0.2 μL BSA, and certified DNA-free PCR water, according to the following procedures: 95 °C for 2 min, 32 cycles of 95 °C for 30 s, 53 °C for 30 s and 72 °C for 30 s, and 72 °C for 5 min. PCR amplifications were all performed on the ABI GeneAmp 9700 PCR system (Applied Biosystems, Waltham, MA, USA). ..

Article Title: Microbially produced vitamin B12 contributes to the lipid-lowering effect of silymarin.
Article Snippet: .. PCR reactions were performed in triplicate in 20μL mixtures containing 4μL of 5 × FastPfu Buffer, 2μL of 2.5mM dNTPs, 0.8μL of each primer (5μM), 0.4μL of FastPfu Polymerase (Transgen Biotech Co. Ltd., Beijing, China), and 10 ng of template DNA with the following settings: 95 °C for 3min, followed by 27 cycles at 95 °C for 30 s, 55 °C for 40 s, and 72 °C for 45 s and a final extension at 72 °C for 10min. .. The resulted PCR products were extracted from a 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences Co. Ltd., Union City, CA, USA), and further quantified using QuantiFluorTM-ST (Promega Co. Ltd., USA).

Amplification:

Article Title: Acidification suppresses the natural capacity of soil microbiome to fight pathogenic Fusarium infections.
Article Snippet: .. The amplification reactions were carried out in a total volume of 20μL, which consisted of 4μL of 5× FastPfu Buffer, 2μL of 2.5mM dNTPs, 0.8μL of both the forward and reverse primers, 10 ng of template DNA, and 0.4μL of FastPfu DNA Polymerase (TransGen Biotech., China). ..

Article Title: Fine root litter quality regulates soil carbon storage efficiency in subtropical forest soils
Article Snippet: .. Amplification reactions were carried out in 4 μL of 5 FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer, 10 ng of template DNA, and 0.4 μL of FastPfu DNA Polymerase (TransGen Biotech., China; total volume 20 μL). .. Amplification was carried out on an ABI GeneAmp 9 700 Thermal Cycler (Thermo Fisher Scientific, USA).

Article Title: Thinning alters the network patterns and keystone taxa of rhizosphere soil microbial communities in Chinese fir plantation
Article Snippet: The composition and structure of microorganisms, which are affected by management practices, play a crucial role in controlling the function of forest ecosystems.. Although the response of microbial communities to thinning has been well studied, the effect of thinning on the cooccurrence patterns of soil bacteria and fungi, especially in rhizosphere soil, remains unclear.. In this study, we investigated the bacterial and fungal communities in rhizosphere and bulk soil of Chinese fir (Cunninghamia lanceolata [Lamb.]

Article Title: Acidification suppresses the natural capacity of soil microbiome to fight pathogenic Fusarium infections
Article Snippet: .. The amplification reactions were carried out in a total volume of 20 μL, which consisted of 4 μL of 5× FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of both the forward and reverse primers, 10 ng of template DNA, and 0.4 μL of FastPfu DNA Polymerase (TransGen Biotech., China). ..



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