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fairplay ® ii microarray labeling kit  (Agilent technologies)


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    Structured Review

    Agilent technologies fairplay ® ii microarray labeling kit
    Fairplay ® Ii Microarray Labeling Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fairplay+ii+microarray+labelling+kit/pmc02175387-81-0-9
    Average 90 stars, based on 1 article reviews
    fairplay ® ii microarray labeling kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Genome-wide expression analysis of roxarsone-stimulated growth of broiler chickens (Gallus gallus).
    Article Snippet: Abbreviations: aa-dUTP, 5-(3-)-2′deoxyuridine-5′-tr albumin; DMSO, dimethyl sulfoxide; GO, gene ontolog ORF, open reading frame; PMT, photomultiplier tube; Q rapid amplification cDNA ends; SDS, sodium lauryl sulfa ⁎ Corresponding author at: Central Veterinary Res United Arab Emirates.. Tel.. : +971 4 3375165; fax: +97 E-mail address: lichanglv@mbg.ae (C. Li).

    Microarray:

    Article Title: Genome-wide expression analysis of roxarsone-stimulated growth of broiler chickens (Gallus gallus).
    Article Snippet: Abbreviations: aa-dUTP, 5-(3-)-2′deoxyuridine-5′-tr albumin; DMSO, dimethyl sulfoxide; GO, gene ontolog ORF, open reading frame; PMT, photomultiplier tube; Q rapid amplification cDNA ends; SDS, sodium lauryl sulfa ⁎ Corresponding author at: Central Veterinary Res United Arab Emirates.. Tel.. : +971 4 3375165; fax: +97 E-mail address: lichanglv@mbg.ae (C. Li).

    Article Title: Proteomic Identification of Putative Biomarkers of Radiotherapy Resistance: A Possible Role for the 26S Proteasome?
    Article Snippet: .. Briefly, indirect complementary DNA (cDNA) labeling was performed using the FairPlay II Microarray Labeling Kit (252006; Stratagene, La Jolla, CA) using Cy3 and Cy5 monofunctional reactive dyes (PA23001 and PA25001; Amersham Biosciences, Bucks, UK). .. Labeled cDNA samples were pooled, combined with 2 μl of 1 μg/ml human Cot-1 DNA (15279011; Invitrogen, Paisley, UK), 2 μl of 1 μg/μl Oligo d(A) (POLYA.GF; Invitrogen), 2 μl of 1 μl/μl yeast tRNA (15401011; Invitrogen), and 390 μl of 10 mM Tris base (pH 8.5) and concentrated to 5 μl using a Microcon YM-100 Centrifugal Filter Device (42413; Millipore, Watford, UK) according to the manufacturer's protocol.

    Article Title: The absence of the luxS gene increases swimming motility and flagella synthesis in Escherichia coli K12.
    Article Snippet: Despite the significant role of S-ribosylhomocysteinase (LuxS) in the activated methyl cycle pathway and quorum sensing, the connectivity between luxS and other cellular functions remains incomplete.. Herein, we show that luxS deletion significantly increases swimming motility and flagella synthesis in Escherichia coli K12 using motility, transcriptome, and scanning electron microscopy assays.. Further, based on the transcriptome and network component analyses, and known regulatory relations, we propose a conceptual genetic regulatory network underlying the increased flagella synthesis in response to luxS deletion.

    Labeling:

    Article Title: Genome-wide expression analysis of roxarsone-stimulated growth of broiler chickens (Gallus gallus).
    Article Snippet: Abbreviations: aa-dUTP, 5-(3-)-2′deoxyuridine-5′-tr albumin; DMSO, dimethyl sulfoxide; GO, gene ontolog ORF, open reading frame; PMT, photomultiplier tube; Q rapid amplification cDNA ends; SDS, sodium lauryl sulfa ⁎ Corresponding author at: Central Veterinary Res United Arab Emirates.. Tel.. : +971 4 3375165; fax: +97 E-mail address: lichanglv@mbg.ae (C. Li).

    Article Title: Proteomic Identification of Putative Biomarkers of Radiotherapy Resistance: A Possible Role for the 26S Proteasome?
    Article Snippet: .. Briefly, indirect complementary DNA (cDNA) labeling was performed using the FairPlay II Microarray Labeling Kit (252006; Stratagene, La Jolla, CA) using Cy3 and Cy5 monofunctional reactive dyes (PA23001 and PA25001; Amersham Biosciences, Bucks, UK). .. Labeled cDNA samples were pooled, combined with 2 μl of 1 μg/ml human Cot-1 DNA (15279011; Invitrogen, Paisley, UK), 2 μl of 1 μg/μl Oligo d(A) (POLYA.GF; Invitrogen), 2 μl of 1 μl/μl yeast tRNA (15401011; Invitrogen), and 390 μl of 10 mM Tris base (pH 8.5) and concentrated to 5 μl using a Microcon YM-100 Centrifugal Filter Device (42413; Millipore, Watford, UK) according to the manufacturer's protocol.

    Article Title: Options available for profiling small samples: a review of sample amplification technology when combined with microarray profiling
    Article Snippet: .. For indirect labeling, the latest version of a commonly used kit (Fairplay II, Stratagene) claims that as little as 2 μg of total RNA (a minimum of ∼7 × 10 4 cells) is required. .. In addition, another brand allows the use of 3 μg total RNA (Atlas Powerscript Kit, Clontech).

    Article Title: The absence of the luxS gene increases swimming motility and flagella synthesis in Escherichia coli K12.
    Article Snippet: Despite the significant role of S-ribosylhomocysteinase (LuxS) in the activated methyl cycle pathway and quorum sensing, the connectivity between luxS and other cellular functions remains incomplete.. Herein, we show that luxS deletion significantly increases swimming motility and flagella synthesis in Escherichia coli K12 using motility, transcriptome, and scanning electron microscopy assays.. Further, based on the transcriptome and network component analyses, and known regulatory relations, we propose a conceptual genetic regulatory network underlying the increased flagella synthesis in response to luxS deletion.

    Incubation:

    Article Title: Genome-wide expression analysis of roxarsone-stimulated growth of broiler chickens (Gallus gallus).
    Article Snippet: Abbreviations: aa-dUTP, 5-(3-)-2′deoxyuridine-5′-tr albumin; DMSO, dimethyl sulfoxide; GO, gene ontolog ORF, open reading frame; PMT, photomultiplier tube; Q rapid amplification cDNA ends; SDS, sodium lauryl sulfa ⁎ Corresponding author at: Central Veterinary Res United Arab Emirates.. Tel.. : +971 4 3375165; fax: +97 E-mail address: lichanglv@mbg.ae (C. Li).

    other:

    Article Title: Transcriptomic and Functional Analysis of NaCl-Induced Stress in Enterococcus faecalis
    Article Snippet: The concentrations of the RNA samples were measured by using the NanoDrop (NanoDrop Technologies), and the quality was assessed by using the RNA 600 Nano LabChip kit and the Bioanalyzer 2100 (Agilent Technologies).

    Article Title: Loss of housekeeping selenoprotein expression in mouse liver modulates lipoprotein metabolism
    Article Snippet: Fairplay ® II Microarray Labeling kit was obtained from Stratagene (La Jolla, CA, USA), RNA Storage Solution from Ambion Inc. (Austin, TX, USA), MinElute column from Qiagen (Valencia, CA, USA) and SYBR green supermix from Bio Rad Laboratories (Hercules, CA, USA).

    Isolation:

    Article Title: The absence of the luxS gene increases swimming motility and flagella synthesis in Escherichia coli K12.
    Article Snippet: Despite the significant role of S-ribosylhomocysteinase (LuxS) in the activated methyl cycle pathway and quorum sensing, the connectivity between luxS and other cellular functions remains incomplete.. Herein, we show that luxS deletion significantly increases swimming motility and flagella synthesis in Escherichia coli K12 using motility, transcriptome, and scanning electron microscopy assays.. Further, based on the transcriptome and network component analyses, and known regulatory relations, we propose a conceptual genetic regulatory network underlying the increased flagella synthesis in response to luxS deletion.

    Mutagenesis:

    Article Title: The absence of the luxS gene increases swimming motility and flagella synthesis in Escherichia coli K12.
    Article Snippet: Despite the significant role of S-ribosylhomocysteinase (LuxS) in the activated methyl cycle pathway and quorum sensing, the connectivity between luxS and other cellular functions remains incomplete.. Herein, we show that luxS deletion significantly increases swimming motility and flagella synthesis in Escherichia coli K12 using motility, transcriptome, and scanning electron microscopy assays.. Further, based on the transcriptome and network component analyses, and known regulatory relations, we propose a conceptual genetic regulatory network underlying the increased flagella synthesis in response to luxS deletion.

    Synthesized:

    Article Title: The absence of the luxS gene increases swimming motility and flagella synthesis in Escherichia coli K12.
    Article Snippet: Despite the significant role of S-ribosylhomocysteinase (LuxS) in the activated methyl cycle pathway and quorum sensing, the connectivity between luxS and other cellular functions remains incomplete.. Herein, we show that luxS deletion significantly increases swimming motility and flagella synthesis in Escherichia coli K12 using motility, transcriptome, and scanning electron microscopy assays.. Further, based on the transcriptome and network component analyses, and known regulatory relations, we propose a conceptual genetic regulatory network underlying the increased flagella synthesis in response to luxS deletion.

    Reverse Transcription:

    Article Title: Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon.
    Article Snippet: .. RNA was reverse transcribed and labelled with either Cy3 or Cy5 using the FAIRPLAY II cDNA labelling kit (Stratagene) according to the manufacturer's instructions. ..



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    Image Search Results


    The effect of NaCl on the expression of EF0282, EF1211 and EF2642 as quantified by QPCR (□; Pfaffl method), and by microarray (▪).

    Journal: PLoS ONE

    Article Title: Transcriptomic and Functional Analysis of NaCl-Induced Stress in Enterococcus faecalis

    doi: 10.1371/journal.pone.0094571

    Figure Lengend Snippet: The effect of NaCl on the expression of EF0282, EF1211 and EF2642 as quantified by QPCR (□; Pfaffl method), and by microarray (▪).

    Article Snippet: The concentrations of the RNA samples were measured by using the NanoDrop (NanoDrop Technologies), and the quality was assessed by using the RNA 600 Nano LabChip kit and the Bioanalyzer 2100 (Agilent Technologies). cDNA was synthesized and labeled with the Fairplay II Microarray labeling kit (Stratagene), with modifications as previously described .

    Techniques: Expressing, Microarray