facswash buffer (Danaher Inc)

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Danaher Inc facswash buffer
Facswash Buffer, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 474 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facswash+buffer/FETAL+BOVINE+SERUM-CHAR+AUSTRALIAN%2C+AUSTRALIAN+FILTERED%2C+500+ML/pmc10198946-153-8-18
Average 96 stars, based on 474 article reviews

facswash buffer - by Bioz Stars, 2026-09

96/100 stars

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Multiple Displacement Amplification:

Article Title: Overexpression of VIRMA confers vulnerability to breast cancers via the m 6 A-dependent regulation of unfolded protein response
Article Snippet: SKBR3, MDA-MB-231, MDA-MB-453, MCF7, AU565, HS578T, and HEK293T cells were obtained from the American Tissue Culture Collection (ATCC). .. SKBR3, MDA-MB-231, and MDA-MB-453 cells were maintained in RPMI media (ThermoFisher cat# 22400089) supplemented with 10% fetal bovine serum (GE Life Sciences cat# SH30084.03). .. AU565 and HS578T cells were cultured in DMEM media and supplements mentioned above with the addition of 1 mM sodium pyruvate (Gibco cat# 11360-070) and 10 μg/mL of insulin (Novo nordisk cat#1331415), respectively.

Cell Culture:

Article Title: Comparative Interactome Analysis of α-arrestin Families in Human and Drosophila
Article Snippet: .. To obtain the media containing lentivirus, HEK293 cells were cultured in DMEM containing 4.5 g/L glucose (SH30243.01; Hyclone) supplemented with 10% FBS (SH30084.03; Hyclone) and 1% penicillin-streptomycin. ..

Transfection:

Article Title: Overexpression of VIRMA confers vulnerability to breast cancers via the m 6 A-dependent regulation of unfolded protein response
Article Snippet: Media was changed 3–4 h post transfection and the cells were grown in the 37 °C incubator with 5% CO 2 for 48 h. Cells were harvested for assessment of transfection efficiency, RT-qPCR and cell proliferation assay using the CCK8 kit (Abcam cat# ab228554). .. For transfection efficiency analysis, cells were resuspended in FACSWash Buffer (1 × PBS containing 2.5% Fetal Bovine Serum (HyCloneTM, cat# SH30084.03), 2 mM EDTA (ThermoFisher, cat# AM9261), 0.01% sodium azide (Sigma, cat# 71289-5G)) containing 0.05μg/mL DAPI (4,6-diamidino-2-phenylindole dihydrochloride, Invitrogen, cat# D1306) for live/dead discrimination. .. Samples were acquired on LSR Fortessa (BD) to determine the percentage of GFP +ve cells and data were analysed using Flowjo X (BD).

other:

Article Title: rhMFGE8 Attenuates Endothelial-to-Mesenchymal Transition in Acute Lung Injury Induced by Lipopolysaccharide Through the BMP/Smad1/4/5/Snail Signalling Pathway
Article Snippet: 2.2 Cell culture Human lung microvascular endothelial cells (HLMECs) were purchased from ScienCell (ScienCell Research Laboratories, Inc., CA, USA, cat. no. 3000) and stored at the College of Life Science.

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