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irdye blocking buffer  (Rockland Immunochemicals)


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    Rockland Immunochemicals irdye blocking buffer
    Irdye Blocking Buffer, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+buffer/FACS+and+IF+Staining+Buffer+with+Goat+Serum/pmc04666965-85-4-7
    Average 93 stars, based on 2 article reviews
    irdye blocking buffer - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: AMP-activated protein kinase inhibits transforming growth factor-β-mediated vascular smooth muscle cell growth: implications for a Smad-3-dependent mechanism
    Article Snippet: All primary antibodies were purchased from Abcam (Cambridge, MA) or Cell Signaling (Danvers, MA), were diluted 1:1,000 for In-Cell Western or 1:500 for immunofluorescence detected by flow cytometry and iCys laser-scanning cytometric (LSC) analysis, and were targeted against the following: Smad2, pSmad2 Thr 8 , Smad3, pSmad3 Ser 423/425 , Smad7; cyclin D, cyclin E, cyclin-dependent kinase (CDK) 2, CDK4, CDK6, p21, p27; α-tubulin, and β-actin. .. Antibodies were diluted in IRDye blocking buffer (Rockland, Gilbertsville, PA) or 1% BSA in PBS (Gemini, Stoneham, MA). .. IRDye secondary antibodies (1:1,000; Rockland) and FITC- or Texas red-conjugated secondary antibodies (1:10,000; Rockland) were used for protein detection while Draq 5 (1:10,000; Cell Signaling) and Sapphire 700 (1:1,000; Li-Cor, Lincoln, NE) were used for DNA staining and protein normalization, respectively.

    Incubation:

    Article Title: Nanoparticle-based artificial RNA silencing machinery for antiviral therapy
    Article Snippet: .. In a typical test, RNA substrates (0.5 μg) were incubated with nanozyme (0.034 nM), DNA-NPs (0.034 nM), control nanozyme (0.034 nM), control DNA-NPs (0.034 nM), or particle-free RNase A (0.408 nM) in a phosphate buffered saline solution (11 μL; phosphate, 10 mM; NaCl, 0.138 M; KCl, 0.027 M; pH 7.4) for 15 min. Then the formaldehyde loading buffer (11 μL, purchased from Londa Rockland, Inc.) was added to denature the RNA products, and the resulting solution was heated at 65 °C for 11 min and then immediately placed on ice for 2 min before loading onto a 2% agarose/formaldehyde denaturing gel (10×3-(N-morpholino) propanesulfonic acid (denoted as MOPS) buffer, 5 mL: RNase free water, 45 mL; agarose, molecular biology grade, 1.0 g; and 37% formaldehyde solution, 0.9 mL). ..

    Control:

    Article Title: Nanoparticle-based artificial RNA silencing machinery for antiviral therapy
    Article Snippet: .. In a typical test, RNA substrates (0.5 μg) were incubated with nanozyme (0.034 nM), DNA-NPs (0.034 nM), control nanozyme (0.034 nM), control DNA-NPs (0.034 nM), or particle-free RNase A (0.408 nM) in a phosphate buffered saline solution (11 μL; phosphate, 10 mM; NaCl, 0.138 M; KCl, 0.027 M; pH 7.4) for 15 min. Then the formaldehyde loading buffer (11 μL, purchased from Londa Rockland, Inc.) was added to denature the RNA products, and the resulting solution was heated at 65 °C for 11 min and then immediately placed on ice for 2 min before loading onto a 2% agarose/formaldehyde denaturing gel (10×3-(N-morpholino) propanesulfonic acid (denoted as MOPS) buffer, 5 mL: RNase free water, 45 mL; agarose, molecular biology grade, 1.0 g; and 37% formaldehyde solution, 0.9 mL). ..

    Saline:

    Article Title: Nanoparticle-based artificial RNA silencing machinery for antiviral therapy
    Article Snippet: .. In a typical test, RNA substrates (0.5 μg) were incubated with nanozyme (0.034 nM), DNA-NPs (0.034 nM), control nanozyme (0.034 nM), control DNA-NPs (0.034 nM), or particle-free RNase A (0.408 nM) in a phosphate buffered saline solution (11 μL; phosphate, 10 mM; NaCl, 0.138 M; KCl, 0.027 M; pH 7.4) for 15 min. Then the formaldehyde loading buffer (11 μL, purchased from Londa Rockland, Inc.) was added to denature the RNA products, and the resulting solution was heated at 65 °C for 11 min and then immediately placed on ice for 2 min before loading onto a 2% agarose/formaldehyde denaturing gel (10×3-(N-morpholino) propanesulfonic acid (denoted as MOPS) buffer, 5 mL: RNase free water, 45 mL; agarose, molecular biology grade, 1.0 g; and 37% formaldehyde solution, 0.9 mL). ..



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    Image Search Results


    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Journal: bioRxiv

    Article Title: A Single-Cell Atlas of the Mouse Dural Meninges Reveals Pervasive Sex Differences Across Cellular Compartments

    doi: 10.64898/2026.05.12.721894

    Figure Lengend Snippet: A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Article Snippet: The cell pellets were resuspended in ice-cold FACS buffer (0.1M PBS; 1mM EDTA (Fisher Scientific) and 1% BSA (Equitech-Bio; BAH70); pH 7.4).

    Techniques: Isolation, Expressing, Quantitative Proteomics