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facs buffer  (Bio-Rad)


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    Bio-Rad facs buffer
    Facs Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 5501 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+buffer/TC20+Automated+Cell+Counter/pm41968960-153-12-28
    Average 97 stars, based on 5501 article reviews
    facs buffer - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    other:

    Article Title: Wnt3-mediated fibrosis and carcinogenesis of lung squamous cell carcinoma in idiopathic pulmonary fibrosis
    Article Snippet: TC20 Automated Cell Counter , Bio-Rad , N/A.

    Article Title: Wnt3-mediated fibrosis and carcinogenesis of lung squamous cell carcinoma in idiopathic pulmonary fibrosis
    Article Snippet: Cells were counted using a TC20 Automated Cell Counter (Bio-Rad) and seeded into 6-well plates.

    Article Title: Characterization of novel macrophage cell lines derived from goat and sheep blood and their susceptibility to ruminant virus infections.
    Article Snippet: At each passage, cells were detached using TrypLE Express Solution (Thermo Fisher Scienti c, Waltham, MA), and the number of harvested cells was measured using a Bio-Rad TC20 automated cell counter.

    Article Title: IL-15 and IL-21 synergy improves anti-tumor efficacy of iPSC-derived cytotoxic T cells in solid tumors.
    Article Snippet: A TC20 automated cell counter (Bio-Rad, USA) was used to count iCAR-T cells.

    Article Title: Protocol for culturing healthy primary endothelial cells isolated from research-grade human corneoscleral donor tissue
    Article Snippet: TC20 Automated Cell Counter , Bio-Rad , Cat. No.: 1450102.

    Isolation:

    Article Title: Enable CAR T cell immunotherapy in glioblastoma by modifying its microenvironment via oncolytic adenovirus encoding bispecific T cell engager
    Article Snippet: CD8+ T cells were isolated from human PBMCs using the EasySep Human CD8+ T cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada). .. After isolation, cells were counted using a TC20 automated cell counter (Bio-Rad, Hercules, CA). .. For cell expansion, Dynabeads Human T-Activator CD3/CD28 for T cell expansion and activation were added to the CD8+ T cells and cultured with IL-2 (PEPROTech, 100 IU/mL; Cranbury, NJ) in complete CTS OpTmizer T cell expansion SFM (Thermo Fisher Scientific).

    Flow Cytometry:

    Article Title: Distinct cytokines regulate gene expression and anti-tumor activity in regenerated CD8 + T cells from induced pluripotent stem cells
    Article Snippet: .. The cell numbers were determined using an automated cell counter (TC20 TM , Bio-Rad) or flow cytometry. ..

    Concentration Assay:

    Article Title: Protocol for identifying cell-type-specific genes associated with disease risk using single-cell eQTL data
    Article Snippet: .. Determine cell concentration and viability using TC20 automated cell counter (Bio-Rad) according to the manufacturer’s instructions. ..



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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
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    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were <t>FACS</t> sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.
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    Image Search Results


    A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Journal: bioRxiv

    Article Title: A Single-Cell Atlas of the Mouse Dural Meninges Reveals Pervasive Sex Differences Across Cellular Compartments

    doi: 10.64898/2026.05.12.721894

    Figure Lengend Snippet: A. Scheme of the experiment. Dura from male and female mice were isolated and pooled. CD45+ and CD45- cells were FACS sorted and analyzed using the 10x Chromium Genomic platform. B. UMAP clustering of the major cell types pooled from male and female mice. C. Feature plots of expression of the major markers identifying major dural cells types. D. UMAP clustering of the dural cells from male and female mice based on sex. E. Bubble plot of expression of sex-chromosome genes in male and female samples. F. Quantification of the proportion of major cell types in male and female samples. G. Violin plot of differentially expressed genes (DEG) from pseudo-bulk analysis comparing male and female cells. Colored genes depict significant differential expression. Blue is enriched in male sample; orange is enriched in female sample. H. Quantification of the number of DEG, either total, (black), up regulated (red) or down regulated (blue) is each major cell type comparing male and female mice. I. Upset plot comparing the unique and shared DEG between males and females across the major cell types.

    Article Snippet: The cell pellets were resuspended in ice-cold FACS buffer (0.1M PBS; 1mM EDTA (Fisher Scientific) and 1% BSA (Equitech-Bio; BAH70); pH 7.4).

    Techniques: Isolation, Expressing, Quantitative Proteomics