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facs staining buffer  (Rockland Immunochemicals)


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    Structured Review

    Rockland Immunochemicals facs staining buffer
    Facs Staining Buffer, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+buffer/FACS+and+IF+Staining+Buffer+with+BSA/pm39638879-185-8-11
    Average 93 stars, based on 6 article reviews
    facs staining buffer - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    FACS:

    Article Title: Cellular trafficking and fate mapping of cells within the nervous system after in utero hematopoietic cell transplantation.
    Article Snippet: Blood was collected in 3mL of PBS (ThermoFisher, Waltham, MA, USA)+ EDTA (Invitrogen, Waltham, MA, USA). .. The pellet was re-suspended in 100 μL of FACS staining buffer (Rockland Immunochemicals, Limerick, PA, USA) and stained with APC rat antimouse CD45 (BD Pharmingen, Franklin Lakes, NJ, USA) for 30min. .. Remaining plasma and red blood cells (RBC) were lysed for 5min with RBC lysis buffer (G-Biosciences, St. Louis,MO,USA).

    Article Title: A Cre-deleter specific for embryo-derived brain macrophages reveals distinct features of microglia and border macrophages.
    Article Snippet: In brief Tools to target microglia specifically and efficiently from the embryonic development are lacking.. Brioschi et al. generated the Crybb1-Cre line, which recombines in microglia and borderassociated macrophages during the embryonic stage.. Combining Crybb1-Cre and other tools, they resolved embryonicderived versus monocyte-derived BAMs in the mouse cortex.

    Article Title: Selective degradation of pathogenic autoantibodies by lysosomal targeting chimeras for the treatment of myasthenia gravis
    Article Snippet: Cells were then trypsinized with 50 μL/well trypLE (Gibco, 12604-021) for 8-10 minutes and transferred into 96-well FACS plates (Thermo Scientific, 14-245-71). .. Cells were washed with FACS buffer (Rockland, MB-089-0500) and centrifuged twice before final resuspension in FACS buffer and read on the flow cytometer (Agilent, NovoCyte Advanteon). .. Data analysis was completed using FlowJo software and the mean LLDR intensity was reported.

    Staining:

    Article Title: Cellular trafficking and fate mapping of cells within the nervous system after in utero hematopoietic cell transplantation.
    Article Snippet: Blood was collected in 3mL of PBS (ThermoFisher, Waltham, MA, USA)+ EDTA (Invitrogen, Waltham, MA, USA). .. The pellet was re-suspended in 100 μL of FACS staining buffer (Rockland Immunochemicals, Limerick, PA, USA) and stained with APC rat antimouse CD45 (BD Pharmingen, Franklin Lakes, NJ, USA) for 30min. .. Remaining plasma and red blood cells (RBC) were lysed for 5min with RBC lysis buffer (G-Biosciences, St. Louis,MO,USA).

    Article Title: A Cre-deleter specific for embryo-derived brain macrophages reveals distinct features of microglia and border macrophages.
    Article Snippet: In brief Tools to target microglia specifically and efficiently from the embryonic development are lacking.. Brioschi et al. generated the Crybb1-Cre line, which recombines in microglia and borderassociated macrophages during the embryonic stage.. Combining Crybb1-Cre and other tools, they resolved embryonicderived versus monocyte-derived BAMs in the mouse cortex.

    Article Title: Evaluation of vaccine candidates against Rhodococcus equi in BALB/c mice infection model: cellular and humoral immune responses
    Article Snippet: After 16 h of stimulation, breamectin A (10 μg/mL, 423303, Biolegend, California, USA) was added and incubated for 6 h. The cells were collected and stained with PerCP-conjugated anti-mouse CD4 (100431, Biolegend) and FITC-conjugated anti-mouse CD8a (100803, Biolegend) antibodies for 30 min at 4 °C in the dark. .. The cells were washed twice with cold FCM staining buffer (MB-089-0500, Rockland, Limerick, Ireland), fixed and permeabilized with FIX&PERM solution (GAS-002 M, Nordic Mubio) for 20 min, and then washed with perm wash buffer. .. Then, the cells were stained with APC-conjugated anti-mouse IFN-γ (505809, Biolegend) and PE-conjugated anti-mouse TNF-α (506305, Biolegend) at 4 °C for 20 min in the dark.

    Flow Cytometry:

    Article Title: A Cre-deleter specific for embryo-derived brain macrophages reveals distinct features of microglia and border macrophages.
    Article Snippet: In brief Tools to target microglia specifically and efficiently from the embryonic development are lacking.. Brioschi et al. generated the Crybb1-Cre line, which recombines in microglia and borderassociated macrophages during the embryonic stage.. Combining Crybb1-Cre and other tools, they resolved embryonicderived versus monocyte-derived BAMs in the mouse cortex.

    Article Title: Selective degradation of pathogenic autoantibodies by lysosomal targeting chimeras for the treatment of myasthenia gravis
    Article Snippet: Cells were then trypsinized with 50 μL/well trypLE (Gibco, 12604-021) for 8-10 minutes and transferred into 96-well FACS plates (Thermo Scientific, 14-245-71). .. Cells were washed with FACS buffer (Rockland, MB-089-0500) and centrifuged twice before final resuspension in FACS buffer and read on the flow cytometer (Agilent, NovoCyte Advanteon). .. Data analysis was completed using FlowJo software and the mean LLDR intensity was reported.

    Single Cell:

    Article Title: A Cre-deleter specific for embryo-derived brain macrophages reveals distinct features of microglia and border macrophages.
    Article Snippet: In brief Tools to target microglia specifically and efficiently from the embryonic development are lacking.. Brioschi et al. generated the Crybb1-Cre line, which recombines in microglia and borderassociated macrophages during the embryonic stage.. Combining Crybb1-Cre and other tools, they resolved embryonicderived versus monocyte-derived BAMs in the mouse cortex.

    Blocking Assay:

    Article Title: A Cre-deleter specific for embryo-derived brain macrophages reveals distinct features of microglia and border macrophages.
    Article Snippet: In brief Tools to target microglia specifically and efficiently from the embryonic development are lacking.. Brioschi et al. generated the Crybb1-Cre line, which recombines in microglia and borderassociated macrophages during the embryonic stage.. Combining Crybb1-Cre and other tools, they resolved embryonicderived versus monocyte-derived BAMs in the mouse cortex.

    Incubation:

    Article Title: A Cre-deleter specific for embryo-derived brain macrophages reveals distinct features of microglia and border macrophages.
    Article Snippet: In brief Tools to target microglia specifically and efficiently from the embryonic development are lacking.. Brioschi et al. generated the Crybb1-Cre line, which recombines in microglia and borderassociated macrophages during the embryonic stage.. Combining Crybb1-Cre and other tools, they resolved embryonicderived versus monocyte-derived BAMs in the mouse cortex.

    other:

    Article Title: Evaluation of vaccine candidates against Rhodococcus equi in BALB/c mice infection model: cellular and humoral immune responses.
    Article Snippet: Then, the cells were stained with APC-conjugated anti-mouse IFN-γ (505809, Biolegend) and PE-conjugated anti-mouse TNF-α (506305, Biolegend) at 4 °C for 20 min in the dark.



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