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Becton Dickinson facs-wash buffer
Facs Wash Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+buffer/facs+wash+buffer/pm34114693-86-6-18
Average 90 stars, based on 1 article reviews
facs-wash buffer - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Incubation:

Article Title: SMRT and NCoR1 fine-tune inflammatory versus tolerogenic balance in dendritic cells by differentially regulating STAT3 signaling
Article Snippet: .. After incubation the cells were washed twice with FACS wash buffer and then acquired for differential expression analysis using LSRII fortessa flow cytometer (BD Biosciences). .. The acquired data was analyzed using FlowJo-X software (Treestar).

Article Title: Cytomegalovirus-specific T cell immunity reconstitution following allogeneic hematopoietic stem cell transplantation: a pilot stduy
Article Snippet: After stimulation, cells were Page 5/26 collected, washed with phosphate-buffered saline (PBS), and incubated in PBS with Live/Dead Fixable Viability Dye Brilliant Violet 510 (BD Bioscience) for 15 min. .. Afterward, the cells were washed using FACS wash buffer (BD Biosciences) and incubated in the dark for 30 min with a uorescent antibody combination comprising anti-CD14 uorescein isothiocyanate (FITC, BD Biosciences), anti-CD4 APC/Cy7 (Biolegend), and anti-CD8 PerCP/Cy5.5 (Biolegend). .. Following a double wash with FACS wash buffer, the cells were permeabilized for 20 min using 1× Cyto x solution (Biolegend), washed twice with Cytoperm buffer (Biolegend), and subsequently stained with a uorescent antibody mixture containing anti-IL-2 PE (Biolegend), anti-IFN-γ APC (Biolegend), anti-TNF-α Brilliant Violet 405 (Biolegend), and anti-macrophage in ammatory protein (MIP)-1β PE/Cy7 (BD Biosciences) in Cytoperm buffer in the dark for 30 min.

FACS:

Article Title: SMRT and NCoR1 fine-tune inflammatory versus tolerogenic balance in dendritic cells by differentially regulating STAT3 signaling
Article Snippet: .. After incubation the cells were washed twice with FACS wash buffer and then acquired for differential expression analysis using LSRII fortessa flow cytometer (BD Biosciences). .. The acquired data was analyzed using FlowJo-X software (Treestar).

Article Title: Cytomegalovirus-specific T cell immunity reconstitution following allogeneic hematopoietic stem cell transplantation: a pilot stduy
Article Snippet: After stimulation, cells were Page 5/26 collected, washed with phosphate-buffered saline (PBS), and incubated in PBS with Live/Dead Fixable Viability Dye Brilliant Violet 510 (BD Bioscience) for 15 min. .. Afterward, the cells were washed using FACS wash buffer (BD Biosciences) and incubated in the dark for 30 min with a uorescent antibody combination comprising anti-CD14 uorescein isothiocyanate (FITC, BD Biosciences), anti-CD4 APC/Cy7 (Biolegend), and anti-CD8 PerCP/Cy5.5 (Biolegend). .. Following a double wash with FACS wash buffer, the cells were permeabilized for 20 min using 1× Cyto x solution (Biolegend), washed twice with Cytoperm buffer (Biolegend), and subsequently stained with a uorescent antibody mixture containing anti-IL-2 PE (Biolegend), anti-IFN-γ APC (Biolegend), anti-TNF-α Brilliant Violet 405 (Biolegend), and anti-macrophage in ammatory protein (MIP)-1β PE/Cy7 (BD Biosciences) in Cytoperm buffer in the dark for 30 min.

Article Title: IL-10-Secreting CD8 + T Cells Specific for Human Cytomegalovirus (HCMV): Generation, Maintenance and Phenotype
Article Snippet: The cells were washed and resuspended in a mix of intracellular antibodies (CD69 Pacific Blue, 4-1BB PE-Cy5, IL-10 PE, CD4 BV605, CD8 BV570 (Biolegend) and IFNγ BV786 (BD Biosciences, Wokingham, UK)) in solution B (FIX & PERM) for 30 min at 4 °C. .. The cells were washed in FACS wash buffer and resuspended in a final concentration of 2% paraformaldehyde (PFA); the samples were acquired on a BD LSR-Fortessa (BD Biosciences) using FACS Diva software and analyzed with FlowJo software (Treestar, BD Biosciences); the samples were compensated and transformed using the software and then analyzed using the gating strategy shown in . ..

Article Title: SARS-CoV-2 Omicron variant escapes neutralizing antibodies and T cell responses more efficiently than other variants in mild COVID-19 convalescents
Article Snippet: .. Fixed cells were washed, resuspended in FACS wash buffer and data was acquired on BD FACS Symphony. ..

Article Title: Enhancement of SARS-CoV-2 N Antigen-Specific T Cell Functionality by Modulating the Autophagy-Mediated Signal Pathway in Mice
Article Snippet: .. After being simulated for five days, cells were harvested and washed with FACS washing buffer and stained with anti-mouse CD3-PE, anti-mouse CD4-BB700, anti-mouse CD8-PE-Cy7, and fixable viability stain 780 (BD, Franklin Lakes, NJ, USA). ..

Article Title: SARS-CoV-2 Omicron variant escapes neutralizing antibodies and T cell responses more efficiently than other variants in mild COVID-19 convalescents
Article Snippet: A maximum of 1 × 10 7 cells were stained with Fixable Viability Stain 700 (FVS700) (BD Bioscience in a 1:1000 dilution), 5 μL Human Fc block (BD Bioscience) per 2 × 10 6 cells, 1 μg/mL each of RBD and Spike tetramers, 5 μL each of CD21 BV421, IgD BV510, CD10 BV605, CD19 BV711 and CD20 APC-H7, 8 μL of IgG BV786, 2 μL each of CD27 PE-CF594 and CD38 PE-Cy7, 2.5 μL HLA-DR BB515 and 0.5 μL CD3 BB700 (BD Bioscience). .. Cells were washed, resuspended in FACS wash buffer and the data acquired on BD FACSAriaTM III. .. Data analysis was performed using FlowJo version 10.7.1 (TreeStar).

Article Title: mTOR inhibition improves the formation of functional T cell memory following COVID-19 vaccination of kidney transplant recipients
Article Snippet: .. Fixed cells were washed, resuspended in FACS wash buffer and data was acquired on BD FACS Symphony. ..

Quantitative Proteomics:

Article Title: SMRT and NCoR1 fine-tune inflammatory versus tolerogenic balance in dendritic cells by differentially regulating STAT3 signaling
Article Snippet: .. After incubation the cells were washed twice with FACS wash buffer and then acquired for differential expression analysis using LSRII fortessa flow cytometer (BD Biosciences). .. The acquired data was analyzed using FlowJo-X software (Treestar).

Flow Cytometry:

Article Title: SMRT and NCoR1 fine-tune inflammatory versus tolerogenic balance in dendritic cells by differentially regulating STAT3 signaling
Article Snippet: .. After incubation the cells were washed twice with FACS wash buffer and then acquired for differential expression analysis using LSRII fortessa flow cytometer (BD Biosciences). .. The acquired data was analyzed using FlowJo-X software (Treestar).

Concentration Assay:

Article Title: IL-10-Secreting CD8 + T Cells Specific for Human Cytomegalovirus (HCMV): Generation, Maintenance and Phenotype
Article Snippet: The cells were washed and resuspended in a mix of intracellular antibodies (CD69 Pacific Blue, 4-1BB PE-Cy5, IL-10 PE, CD4 BV605, CD8 BV570 (Biolegend) and IFNγ BV786 (BD Biosciences, Wokingham, UK)) in solution B (FIX & PERM) for 30 min at 4 °C. .. The cells were washed in FACS wash buffer and resuspended in a final concentration of 2% paraformaldehyde (PFA); the samples were acquired on a BD LSR-Fortessa (BD Biosciences) using FACS Diva software and analyzed with FlowJo software (Treestar, BD Biosciences); the samples were compensated and transformed using the software and then analyzed using the gating strategy shown in . ..

Software:

Article Title: IL-10-Secreting CD8 + T Cells Specific for Human Cytomegalovirus (HCMV): Generation, Maintenance and Phenotype
Article Snippet: The cells were washed and resuspended in a mix of intracellular antibodies (CD69 Pacific Blue, 4-1BB PE-Cy5, IL-10 PE, CD4 BV605, CD8 BV570 (Biolegend) and IFNγ BV786 (BD Biosciences, Wokingham, UK)) in solution B (FIX & PERM) for 30 min at 4 °C. .. The cells were washed in FACS wash buffer and resuspended in a final concentration of 2% paraformaldehyde (PFA); the samples were acquired on a BD LSR-Fortessa (BD Biosciences) using FACS Diva software and analyzed with FlowJo software (Treestar, BD Biosciences); the samples were compensated and transformed using the software and then analyzed using the gating strategy shown in . ..

Transformation Assay:

Article Title: IL-10-Secreting CD8 + T Cells Specific for Human Cytomegalovirus (HCMV): Generation, Maintenance and Phenotype
Article Snippet: The cells were washed and resuspended in a mix of intracellular antibodies (CD69 Pacific Blue, 4-1BB PE-Cy5, IL-10 PE, CD4 BV605, CD8 BV570 (Biolegend) and IFNγ BV786 (BD Biosciences, Wokingham, UK)) in solution B (FIX & PERM) for 30 min at 4 °C. .. The cells were washed in FACS wash buffer and resuspended in a final concentration of 2% paraformaldehyde (PFA); the samples were acquired on a BD LSR-Fortessa (BD Biosciences) using FACS Diva software and analyzed with FlowJo software (Treestar, BD Biosciences); the samples were compensated and transformed using the software and then analyzed using the gating strategy shown in . ..

Staining:

Article Title: Enhancement of SARS-CoV-2 N Antigen-Specific T Cell Functionality by Modulating the Autophagy-Mediated Signal Pathway in Mice
Article Snippet: .. After being simulated for five days, cells were harvested and washed with FACS washing buffer and stained with anti-mouse CD3-PE, anti-mouse CD4-BB700, anti-mouse CD8-PE-Cy7, and fixable viability stain 780 (BD, Franklin Lakes, NJ, USA). ..

other:

Article Title: Activation of human γδ T cells and NK cells by Staphylococcal enterotoxins requires both monocytes and conventional T cells.
Article Snippet: Stained cells were washed, resuspended in FACS-wash buffer, and acquired using a FACSVerse instrument and FACS-Suite software (both BD Biosciences).



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