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nbp2  (Novus Biologicals)


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    Novus Biologicals nbp2
    Nbp2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/CD68%2FSR-D1+Antibody+(FA-11)+-+BSA+Free/pmc12931916-35-7-4
    Average 94 stars, based on 22 article reviews
    nbp2 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Endothelial JAML inhibits inflammation and atherosclerosis through TRIM25-mediated STAT1 ubiquitination.
    Article Snippet: To detect JAML expression in aortic cells, the sections were co-stained with anti-JAML (1:50, 21302-1-AP, PTG) and antiCD31 (1:50, GB15063-50, Servicebio) antibodies. .. Additionally, immunofluorescence staining was performed using antibodies against α-SMA (1:200, ab5694, Abcam), CD45 (1:100, 20103- 1-AP, PTG), CD68 (1:200, NBP2-33337, Novus), VCAM-1 (1:100, ab134047, Abcam), ICAM-1 (1:100, 10020-1-AP, PTG), and STAT1 (1:100, 10144-2-AP, PTG) to assess their respective expression and localization. .. After washing, the sections were incubated with secondary antibodies at 37°C for 30 min, followed by DAPI staining (ab104139, Abcam).

    Staining:

    Article Title: Endothelial JAML inhibits inflammation and atherosclerosis through TRIM25-mediated STAT1 ubiquitination.
    Article Snippet: To detect JAML expression in aortic cells, the sections were co-stained with anti-JAML (1:50, 21302-1-AP, PTG) and antiCD31 (1:50, GB15063-50, Servicebio) antibodies. .. Additionally, immunofluorescence staining was performed using antibodies against α-SMA (1:200, ab5694, Abcam), CD45 (1:100, 20103- 1-AP, PTG), CD68 (1:200, NBP2-33337, Novus), VCAM-1 (1:100, ab134047, Abcam), ICAM-1 (1:100, 10020-1-AP, PTG), and STAT1 (1:100, 10144-2-AP, PTG) to assess their respective expression and localization. .. After washing, the sections were incubated with secondary antibodies at 37°C for 30 min, followed by DAPI staining (ab104139, Abcam).

    Expressing:

    Article Title: Endothelial JAML inhibits inflammation and atherosclerosis through TRIM25-mediated STAT1 ubiquitination.
    Article Snippet: To detect JAML expression in aortic cells, the sections were co-stained with anti-JAML (1:50, 21302-1-AP, PTG) and antiCD31 (1:50, GB15063-50, Servicebio) antibodies. .. Additionally, immunofluorescence staining was performed using antibodies against α-SMA (1:200, ab5694, Abcam), CD45 (1:100, 20103- 1-AP, PTG), CD68 (1:200, NBP2-33337, Novus), VCAM-1 (1:100, ab134047, Abcam), ICAM-1 (1:100, 10020-1-AP, PTG), and STAT1 (1:100, 10144-2-AP, PTG) to assess their respective expression and localization. .. After washing, the sections were incubated with secondary antibodies at 37°C for 30 min, followed by DAPI staining (ab104139, Abcam).

    Incubation:

    Article Title: p16-expressing microglia and endothelial cells promote tauopathy and neurovascular abnormalities in PS19 mice.
    Article Snippet: Article p16-expressing microglia and endothelial cells promote tauopathy and neurovascular abnormalities in PS19 mice

    Article Title: The Endothelial Cell-Expressed Prion Protein Prnd/Doppel Promotes Neovascularization and Long-term Recovery after Ischemic Stroke
    Article Snippet: .. We added primary antibodies: Prnd (Thermo Fisher Scientific, PIPA5113502), VEGF (Thermo Fisher Scientific, ENP802), CD31 (R&D Systems, AF3628), ZO-1 (Thermo Fisher Scientific, PIPA585256 ), Claudin 5 (Thermo Fisher Scientific, 35-2500), GFAP (Novus, NBP1-05198), CD68 (Novus, NBP2-33337), Neun (Thermo Fisher Scientific, PIPA5143552), TMEM 119 (Novus Biologicals, NBP230551), Laminin (Sigma, L9393), and VE-Cadherin (BD Pharmingen, 550548) to the slides and incubated the slides overnight at 4°C in a moisturized dark box, washed three times (5min per wash with gentle rocking at room temperature) and added secondary antibodies (1:500 dilutions) and incubated at room temperature for 1 hr. ..

    Blocking Assay:

    Article Title: p16-expressing microglia and endothelial cells promote tauopathy and neurovascular abnormalities in PS19 mice.
    Article Snippet: Article p16-expressing microglia and endothelial cells promote tauopathy and neurovascular abnormalities in PS19 mice

    Gentle:

    Article Title: The Endothelial Cell-Expressed Prion Protein Prnd/Doppel Promotes Neovascularization and Long-term Recovery after Ischemic Stroke
    Article Snippet: .. We added primary antibodies: Prnd (Thermo Fisher Scientific, PIPA5113502), VEGF (Thermo Fisher Scientific, ENP802), CD31 (R&D Systems, AF3628), ZO-1 (Thermo Fisher Scientific, PIPA585256 ), Claudin 5 (Thermo Fisher Scientific, 35-2500), GFAP (Novus, NBP1-05198), CD68 (Novus, NBP2-33337), Neun (Thermo Fisher Scientific, PIPA5143552), TMEM 119 (Novus Biologicals, NBP230551), Laminin (Sigma, L9393), and VE-Cadherin (BD Pharmingen, 550548) to the slides and incubated the slides overnight at 4°C in a moisturized dark box, washed three times (5min per wash with gentle rocking at room temperature) and added secondary antibodies (1:500 dilutions) and incubated at room temperature for 1 hr. ..



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    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) Stereomicroscope images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.

    Journal: Science Advances

    Article Title: p75 NTR orchestrates chondrogenesis and mechanoadaptation to preserve temporomandibular joint development and homeostasis

    doi: 10.1126/sciadv.aef7786

    Figure Lengend Snippet: ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) Stereomicroscope images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.

    Article Snippet: For histological and morphometric analysis, pellets were cultured for 3 weeks and first imaged using a stereomicroscope (LEICA M205 FA; Leica Microsystems).

    Techniques: Analysis, Staining, Gene Expression, Western Blot, TUNEL Assay, Proliferation Assay, Derivative Assay, Apoptosis Assay, Single Cell, RNA Sequencing, Real-time Polymerase Chain Reaction