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m205 fa fluorescence stereomicroscope  (Danaher Inc)


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    Structured Review

    Danaher Inc m205 fa fluorescence stereomicroscope
    M205 Fa Fluorescence Stereomicroscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/M205+FA+Fluorescence+Stereo+Microscopes/pmc07959511-480-33-37
    Average 96 stars, based on 1297 article reviews
    m205 fa fluorescence stereomicroscope - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Subchondral bone remodeling in knee osteoarthritis is coupled to lipid mobilization from bone marrow adipocytes
    Article Snippet: .. Whole-mount images of the stained osteochondral chips were acquired using a Leica M205 FA stereomicroscope. ..

    Analysis:

    Article Title: p75 NTR orchestrates chondrogenesis and mechanoadaptation to preserve temporomandibular joint development and homeostasis
    Article Snippet: .. For histological and morphometric analysis, pellets were cultured for 3 weeks and first imaged using a stereomicroscope (LEICA M205 FA; Leica Microsystems). ..

    Cell Culture:

    Article Title: p75 NTR orchestrates chondrogenesis and mechanoadaptation to preserve temporomandibular joint development and homeostasis
    Article Snippet: .. For histological and morphometric analysis, pellets were cultured for 3 weeks and first imaged using a stereomicroscope (LEICA M205 FA; Leica Microsystems). ..

    Microscopy:

    Article Title: H3K18 lactylation promotes VEGFR-3-dependent lymphatic hyperplasia and barrier disruption in metabolic syndrome
    Article Snippet: After 5-min ventilation, the inferior vena cava was ligated, and 800 μL of 30 mg/mL Fluorescein Isothiocyanate-Dextran (Molecular Weight: 500 kDa, Sigma-Aldrich, FD20S) was injected into anterior vena cava to trace the blood vasculature. .. The hearts were arrested in diastole, immersed into PBS, and immediately imaged with a M205 FA stereo microscope (Leica, Germany). .. For mouse hearts that underwent cardiac lymphangiography, cardiac tissues labeled with Qdots within the lymphatic plexus regions were harvested under an M205 FA stereo microscope (Leica, Germany).

    Article Title: Coordination of lignin and pectin remodeling by a NAC transcription factor regulates seed abscission in sesame (Sesamum indicum L.).
    Article Snippet: .. Low-magnification images were then 19 collected using a Leica stereomicroscope (M205FA, Leica) and confocal laser-scanning 20 microscopy images were obtained using a Zeiss LSM 700 (Zen SP3 Black edition). ..

    Article Title: In vivo DNA damage protection during cell migration across confining embryonic tissue environments.
    Article Snippet: .. The embryos were mounted, and single images were captured using a Leica M205 FA fluorescence microscope. .. To visualize the extracellular space between adjacent tissues in live embryos, Neurocan-GFP mRNA injection or 10-kDa dextran red or far red was used (Alexa 546 10,000 Mw dextran, Invitrogen D22911, Alexa 647 10,000 Mw dextran, Invitrogen D22914).

    Confocal:

    Article Title: Coordination of lignin and pectin remodeling by a NAC transcription factor regulates seed abscission in sesame (Sesamum indicum L.).
    Article Snippet: .. Low-magnification images were then 19 collected using a Leica stereomicroscope (M205FA, Leica) and confocal laser-scanning 20 microscopy images were obtained using a Zeiss LSM 700 (Zen SP3 Black edition). ..

    Fluorescence:

    Article Title: A Chiral Virus-Like Nanoplatform That Broadly Mediates Antibiotic Delivery to the Brain for Bacterial Meningitis Therapy.
    Article Snippet: Fluoresence images were acquired using a small animal imaging ystem (Carestream FX PRO, Bruker/Carestream, USA; Ex/Em: 4 of 28 570/625 nm), and the average fluorescence intensity of each organ was quantified via ROI analysis. .. Brain fluorescence intensity was further examined under a stereomicroscope (M205FA, Leica, Germany). ..

    Article Title: In vivo DNA damage protection during cell migration across confining embryonic tissue environments.
    Article Snippet: .. The embryos were mounted, and single images were captured using a Leica M205 FA fluorescence microscope. .. To visualize the extracellular space between adjacent tissues in live embryos, Neurocan-GFP mRNA injection or 10-kDa dextran red or far red was used (Alexa 546 10,000 Mw dextran, Invitrogen D22911, Alexa 647 10,000 Mw dextran, Invitrogen D22914).



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    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) <t>Stereomicroscope</t> images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.
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    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) <t>Stereomicroscope</t> images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.
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    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) <t>Stereomicroscope</t> images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.
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    nefa  (Randox)
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    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) <t>Stereomicroscope</t> images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.
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    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) <t>Stereomicroscope</t> images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.
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    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) <t>Stereomicroscope</t> images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.
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    Danaher Inc leica m205 fa stereomicroscope
    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) <t>Stereomicroscope</t> images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.
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    Randox non esterified fatty acids nefa serum levels
    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) <t>Stereomicroscope</t> images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.
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    Danaher Inc leica m205fa
    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) <t>Stereomicroscope</t> images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.
    Leica M205fa, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) Stereomicroscope images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.

    Journal: Science Advances

    Article Title: p75 NTR orchestrates chondrogenesis and mechanoadaptation to preserve temporomandibular joint development and homeostasis

    doi: 10.1126/sciadv.aef7786

    Figure Lengend Snippet: ( A ) scRNA-seq workflow. Created in BioRender. Kadota, C. (2026) https://BioRender.com/ks47d2b . ( B ) UMAP visualization of cell types in TMJ. Chondrocytes are highlighted in blue. ( C ) Proportion of each cell cluster in p75 +/+ and p75 −/− MCCs. ( D ) Pathway analysis of chondrocytes identified by scRNA-seq. ( E ) Stereomicroscope images and gross pellet size. Scale bar, 500 μm. ( F ) Safranin O staining [Top: Whole-pellet view (10×; scale bar, 500 μm); bottom, 40× view of the dotted area. Scale bar, 100 μm] and quantification of Safranin O–positive area, and ( G ) gene expression analyses ( Acan , Ihh , Sox9 , and Col2a1 ) of p75 +/+ and p75 −/− mBMSCs underwent 3D pellet culture ( N = 6 per group). ( H ) Left: Western blotting of p75 NTR , phosphorylated-AKT, AKT, phosphorylated-p38, and p38 in mBMSCs treated with vehicle or BDNF. Right: Quantification of p-AKT/AKT and p-p38/p38 ratios. ( N = 5 independent experiments). ( I ) Gene expression analyses in p75 +/+ and p75 −/− condyles for p75 NTR , Ntrk2 , Bdnf , Trp53 , Bax , Acan , Ihh , Sox9 , Col2a1 , and Mki67 ( N = 5 per group). IF staining for ( J ) Ki-67 and ( K ) TUNEL and quantification of Ki-67 and TUNEL-positive cells in p75 +/+ and p75 −/− TMJ ( N = 5 per group). Blue, DAPI. Scale bar, 50 μm. ( L ) Cell proliferation assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). ( M ) Apoptosis assay of mBMSCs derived from p75 +/+ and p75 −/− mice ( N = 5 per group). Statistical significance was determined using unpaired two-sided Student’s t test or two-way ANOVA, as appropriate. Bonferroni correction was applied for pairwise post hoc comparisons following two-way ANOVA. Results are represented as the means ± 1 SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. N represents the number of mice or experimental replicates.

    Article Snippet: For histological and morphometric analysis, pellets were cultured for 3 weeks and first imaged using a stereomicroscope (LEICA M205 FA; Leica Microsystems).

    Techniques: Analysis, Staining, Gene Expression, Western Blot, TUNEL Assay, Proliferation Assay, Derivative Assay, Apoptosis Assay, Single Cell, RNA Sequencing, Real-time Polymerase Chain Reaction