Review



nonesterified fatty acid nefa  (Randox)


Bioz Verified Symbol Randox is a verified supplier
Bioz Manufacturer Symbol Randox manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Randox nonesterified fatty acid nefa
    Nonesterified Fatty Acid Nefa, supplied by Randox, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/NEFA/pm41453537-75-17-22
    Average 96 stars, based on 1 article reviews
    nonesterified fatty acid nefa - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Glass:

    Article Title: Individual and Additive Effects of Acute Intake of New Zealand Blackcurrant Powder and Caffeine on High-Intensity Intermittent Running Performance
    Article Snippet: phase HPLC. Detailed results for plasma caffeine and caffeine metabolite concentrations are presented in Appendix A.3 . Serum FFA were analysed using the Randox non-esterified fatty acids kit (FA 115, Randox Laboratories Limited, Crumlin, Northern Ireland) according to the manufacturer’s instructions using a clinical chemistry analyser (RX Daytona plus, Randox Laboratories Limited, Crumlin, Norther

    Article Title: Effect of the Use of Bovine Appeasing Substance on Immunological, Metabolic, and Oxidative Parameters of Postpartum Dairy Cows: Modulation of the Stress Axis
    Article Snippet: Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].. Non-esterified fatty acids (NEFA) were analyzed using Randox® kits (FA115—Colorimetric Method and RB1007—Enzymatic Method, Antrim, UK) [21].. ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).

    Article Title: Multivariate analysis of placental gene expression of adipokines and biochemical markers in cows with and without hyperketonemia.
    Article Snippet: 3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.. 4 NEFA, RANDOX Laboratories Ltd./ref.FA115, Reino Unido.. 5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.

    Article Title: Ketone monoester reduces blood glucose, exogenous CHO oxidation and oxidation efficiency in trained male cyclists when fed 120 g/h of CHO during exercise.
    Article Snippet: Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.. 333 Samples were later analysed for glycerol and non-esterified fatty acids (NEFA) with 334 commercially available enzymatic spectrophotometric assays (RX Daytona+ Analyser; Randox, 335 UK) as per the manufacturer’s instructions (analytical coefficient of variation, CV, <2% and 336 <5%, respectively).. Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.

    High Performance Liquid Chromatography:

    Article Title: Individual and Additive Effects of Acute Intake of New Zealand Blackcurrant Powder and Caffeine on High-Intensity Intermittent Running Performance
    Article Snippet: phase HPLC. Detailed results for plasma caffeine and caffeine metabolite concentrations are presented in Appendix A.3 . Serum FFA were analysed using the Randox non-esterified fatty acids kit (FA 115, Randox Laboratories Limited, Crumlin, Northern Ireland) according to the manufacturer’s instructions using a clinical chemistry analyser (RX Daytona plus, Randox Laboratories Limited, Crumlin, Norther

    Article Title: Effect of the Use of Bovine Appeasing Substance on Immunological, Metabolic, and Oxidative Parameters of Postpartum Dairy Cows: Modulation of the Stress Axis
    Article Snippet: Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].. Non-esterified fatty acids (NEFA) were analyzed using Randox® kits (FA115—Colorimetric Method and RB1007—Enzymatic Method, Antrim, UK) [21].. ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).

    Article Title: Multivariate analysis of placental gene expression of adipokines and biochemical markers in cows with and without hyperketonemia.
    Article Snippet: 3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.. 4 NEFA, RANDOX Laboratories Ltd./ref.FA115, Reino Unido.. 5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.

    Article Title: Ketone monoester reduces blood glucose, exogenous CHO oxidation and oxidation efficiency in trained male cyclists when fed 120 g/h of CHO during exercise.
    Article Snippet: Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.. 333 Samples were later analysed for glycerol and non-esterified fatty acids (NEFA) with 334 commercially available enzymatic spectrophotometric assays (RX Daytona+ Analyser; Randox, 335 UK) as per the manufacturer’s instructions (analytical coefficient of variation, CV, <2% and 336 <5%, respectively).. Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.

    Clinical Proteomics:

    Article Title: Individual and Additive Effects of Acute Intake of New Zealand Blackcurrant Powder and Caffeine on High-Intensity Intermittent Running Performance
    Article Snippet: phase HPLC. Detailed results for plasma caffeine and caffeine metabolite concentrations are presented in Appendix A.3 . Serum FFA were analysed using the Randox non-esterified fatty acids kit (FA 115, Randox Laboratories Limited, Crumlin, Northern Ireland) according to the manufacturer’s instructions using a clinical chemistry analyser (RX Daytona plus, Randox Laboratories Limited, Crumlin, Norther

    Article Title: Effect of the Use of Bovine Appeasing Substance on Immunological, Metabolic, and Oxidative Parameters of Postpartum Dairy Cows: Modulation of the Stress Axis
    Article Snippet: Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].. Non-esterified fatty acids (NEFA) were analyzed using Randox® kits (FA115—Colorimetric Method and RB1007—Enzymatic Method, Antrim, UK) [21].. ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).

    Article Title: Multivariate analysis of placental gene expression of adipokines and biochemical markers in cows with and without hyperketonemia.
    Article Snippet: 3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.. 4 NEFA, RANDOX Laboratories Ltd./ref.FA115, Reino Unido.. 5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.

    Article Title: Ketone monoester reduces blood glucose, exogenous CHO oxidation and oxidation efficiency in trained male cyclists when fed 120 g/h of CHO during exercise.
    Article Snippet: Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.. 333 Samples were later analysed for glycerol and non-esterified fatty acids (NEFA) with 334 commercially available enzymatic spectrophotometric assays (RX Daytona+ Analyser; Randox, 335 UK) as per the manufacturer’s instructions (analytical coefficient of variation, CV, <2% and 336 <5%, respectively).. Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.

    Reversed-phase High-Performance Liquid Chromatography:

    Article Title: Individual and Additive Effects of Acute Intake of New Zealand Blackcurrant Powder and Caffeine on High-Intensity Intermittent Running Performance
    Article Snippet: phase HPLC. Detailed results for plasma caffeine and caffeine metabolite concentrations are presented in Appendix A.3 . Serum FFA were analysed using the Randox non-esterified fatty acids kit (FA 115, Randox Laboratories Limited, Crumlin, Northern Ireland) according to the manufacturer’s instructions using a clinical chemistry analyser (RX Daytona plus, Randox Laboratories Limited, Crumlin, Norther

    Article Title: Effect of the Use of Bovine Appeasing Substance on Immunological, Metabolic, and Oxidative Parameters of Postpartum Dairy Cows: Modulation of the Stress Axis
    Article Snippet: Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].. Non-esterified fatty acids (NEFA) were analyzed using Randox® kits (FA115—Colorimetric Method and RB1007—Enzymatic Method, Antrim, UK) [21].. ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).

    Article Title: Multivariate analysis of placental gene expression of adipokines and biochemical markers in cows with and without hyperketonemia.
    Article Snippet: 3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.. 4 NEFA, RANDOX Laboratories Ltd./ref.FA115, Reino Unido.. 5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.

    Article Title: Ketone monoester reduces blood glucose, exogenous CHO oxidation and oxidation efficiency in trained male cyclists when fed 120 g/h of CHO during exercise.
    Article Snippet: Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.. 333 Samples were later analysed for glycerol and non-esterified fatty acids (NEFA) with 334 commercially available enzymatic spectrophotometric assays (RX Daytona+ Analyser; Randox, 335 UK) as per the manufacturer’s instructions (analytical coefficient of variation, CV, <2% and 336 <5%, respectively).. Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.

    Northern Blot:

    Article Title: Individual and Additive Effects of Acute Intake of New Zealand Blackcurrant Powder and Caffeine on High-Intensity Intermittent Running Performance
    Article Snippet: phase HPLC. Detailed results for plasma caffeine and caffeine metabolite concentrations are presented in Appendix A.3 . Serum FFA were analysed using the Randox non-esterified fatty acids kit (FA 115, Randox Laboratories Limited, Crumlin, Northern Ireland) according to the manufacturer’s instructions using a clinical chemistry analyser (RX Daytona plus, Randox Laboratories Limited, Crumlin, Norther

    Article Title: Effect of the Use of Bovine Appeasing Substance on Immunological, Metabolic, and Oxidative Parameters of Postpartum Dairy Cows: Modulation of the Stress Axis
    Article Snippet: Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].. Non-esterified fatty acids (NEFA) were analyzed using Randox® kits (FA115—Colorimetric Method and RB1007—Enzymatic Method, Antrim, UK) [21].. ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).

    Article Title: Multivariate analysis of placental gene expression of adipokines and biochemical markers in cows with and without hyperketonemia.
    Article Snippet: 3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.. 4 NEFA, RANDOX Laboratories Ltd./ref.FA115, Reino Unido.. 5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.

    Article Title: Ketone monoester reduces blood glucose, exogenous CHO oxidation and oxidation efficiency in trained male cyclists when fed 120 g/h of CHO during exercise.
    Article Snippet: Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.. 333 Samples were later analysed for glycerol and non-esterified fatty acids (NEFA) with 334 commercially available enzymatic spectrophotometric assays (RX Daytona+ Analyser; Randox, 335 UK) as per the manufacturer’s instructions (analytical coefficient of variation, CV, <2% and 336 <5%, respectively).. Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.

    Concentration Assay:

    Article Title: Individual and Additive Effects of Acute Intake of New Zealand Blackcurrant Powder and Caffeine on High-Intensity Intermittent Running Performance
    Article Snippet: phase HPLC. Detailed results for plasma caffeine and caffeine metabolite concentrations are presented in Appendix A.3 . Serum FFA were analysed using the Randox non-esterified fatty acids kit (FA 115, Randox Laboratories Limited, Crumlin, Northern Ireland) according to the manufacturer’s instructions using a clinical chemistry analyser (RX Daytona plus, Randox Laboratories Limited, Crumlin, Norther

    Article Title: Effect of the Use of Bovine Appeasing Substance on Immunological, Metabolic, and Oxidative Parameters of Postpartum Dairy Cows: Modulation of the Stress Axis
    Article Snippet: Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].Serum cortisol (Immulite Cortisol, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) and insulin-like growth factor I-IGF-I (Immulite IGF-1, Siemens Healthcare Diagnostics, Tarrytown, NY, USA) were measured with immunoassay tests that implemented the chemiluminescence method using a commercial kit, with the methodology also being described by Baldacim et al. [20].. Non-esterified fatty acids (NEFA) were analyzed using Randox® kits (FA115—Colorimetric Method and RB1007—Enzymatic Method, Antrim, UK) [21].. ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).ROS analysis in serum was performed using a fluorimetric protocol established by Lebel et al. [22], where 10 μL of serum was incubated with the same amount of 2′,7′- dichlorofluorescein diacetate (DCFH-DA, 7 μM) and 240 μL of sulfate-buffered saline (PBS).

    Article Title: Multivariate analysis of placental gene expression of adipokines and biochemical markers in cows with and without hyperketonemia.
    Article Snippet: 3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.3 β-hidroxibutirato /Rambut, RANDOX Laboratories Ltd./ref.RB1008, Reino Unido.. 4 NEFA, RANDOX Laboratories Ltd./ref.FA115, Reino Unido.. 5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.5 Glucose Liquiform, Labtest Diagnostics S.A., Brazil.

    Article Title: Ketone monoester reduces blood glucose, exogenous CHO oxidation and oxidation efficiency in trained male cyclists when fed 120 g/h of CHO during exercise.
    Article Snippet: Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.Following 332 centrifugation, plasma samples were aliquoted and stored at -80 °C for subsequent analysis.. 333 Samples were later analysed for glycerol and non-esterified fatty acids (NEFA) with 334 commercially available enzymatic spectrophotometric assays (RX Daytona+ Analyser; Randox, 335 UK) as per the manufacturer’s instructions (analytical coefficient of variation, CV, <2% and 336 <5%, respectively).. Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.Note that plasma NEFA and glycerol data are representative of n=7 only due 337 to a catheter fault with one participant.



    Similar Products

    leica m205fa stereo microscope  (Danaher Inc)
    96
    Danaher Inc leica m205fa stereo microscope
    Leica M205fa Stereo Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/M205+FA+Fluorescence+Stereo+Microscopes/pmc13628057-255-8-8
    Average 96 stars, based on 1 article reviews
    leica m205fa stereo microscope - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    stereomicroscope  (Danaher Inc)
    96
    Danaher Inc stereomicroscope
    Stereomicroscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/M205+FA+Fluorescence+Stereo+Microscopes/pmc13633098-285-12-14
    Average 96 stars, based on 1 article reviews
    stereomicroscope - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    fa cells lines  (Qiagen)
    99
    Qiagen fa cells lines
    Fa Cells Lines, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/RNeasy+Mini+Kit/pm42816536-306-11-26
    Average 99 stars, based on 1 article reviews
    fa cells lines - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    e e farnesol fa mp biomedicals  (Valiant Co Ltd)
    90
    Valiant Co Ltd e e farnesol fa mp biomedicals
    E E Farnesol Fa Mp Biomedicals, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/Farnesol/pm42789392-242-12-14
    Average 90 stars, based on 1 article reviews
    e e farnesol fa mp biomedicals - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    fluorescence stereomicroscope  (Danaher Inc)
    96
    Danaher Inc fluorescence stereomicroscope
    Fluorescence Stereomicroscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/M205+FA+Fluorescence+Stereo+Microscopes/pm42776840-751-43-46
    Average 96 stars, based on 1 article reviews
    fluorescence stereomicroscope - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    fa 200 mg kg mce hy 16637  (MedChemExpress)
    95
    MedChemExpress fa 200 mg kg mce hy 16637
    Acsm2/ACSM2A is downregulated in renal tubular <t>epithelial</t> <t>cells</t> from three distinct AKI mouse models and in OGD/R-induced cell models. (A) A schematic diagram showing the construction of the IRI-induced AKI mouse model. (B,C) Renal function was assessed by measuring serum creatinine (B) and BUN (C) in a mouse model of IRI-induced AKI (mice n = 5). (D) Transcriptional level of Acsm2 in IRI-induced AKI mouse model (mice n = 5). (E) Western blot analysis of Acsm2 protein expression in a mouse model of IRI-induced AKI. (F) A schematic diagram showing the construction of the FA-induced AKI mouse model. (G,H) Renal function was assessed by measuring serum creatinine (G) and BUN (H) in a mouse model of FA-induced AKI (mice n = 5). (I) Transcriptional level of Acsm2 in FA-induced AKI mouse model (mice n = 5). (J) Western blot analysis of Acsm2 protein expression in a mouse model of FA-induced AKI. (K) A schematic diagram showing the construction of the Cisplatin-induced AKI mouse model. (L,M) Renal function was assessed by measuring serum creatinine (L) and BUN (M) in a mouse model of Cisplatin-induced AKI (mice n = 5). (N) Transcriptional level of Acsm2 in Cisplatin-induced AKI mouse model (mice n = 5). (O) Western blot analysis of Acsm2 protein expression in a mouse model of Cisplatin-induced AKI. (P) Transcriptional level of ACSM2A in OGD/R-induced HK-2 cell models ( n = 3). (Q) Western blot analysis of ACSM2A protein expression in OGD/R-induced HK-2 cell models. (R) Transcriptional level of ACSM2A in OGD/R-induced primary human TECs models ( n = 3). Data are representative of three independent experiments (E, J, O, Q). Error bars are shown as mean ± SD with n = 3 (P, R) or n = 5 (B-D, G-I, L-N) biological replicates. Two-tailed unpaired Student’s t-test (B-D, G-I, L-N, P, R). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.
    Fa 200 Mg Kg Mce Hy 16637, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/Folic+acid/pmc13642285-247-20-23
    Average 95 stars, based on 1 article reviews
    fa 200 mg kg mce hy 16637 - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    fa 45 24 11  (Eppendorf AG)
    96
    Eppendorf AG fa 45 24 11
    Acsm2/ACSM2A is downregulated in renal tubular <t>epithelial</t> <t>cells</t> from three distinct AKI mouse models and in OGD/R-induced cell models. (A) A schematic diagram showing the construction of the IRI-induced AKI mouse model. (B,C) Renal function was assessed by measuring serum creatinine (B) and BUN (C) in a mouse model of IRI-induced AKI (mice n = 5). (D) Transcriptional level of Acsm2 in IRI-induced AKI mouse model (mice n = 5). (E) Western blot analysis of Acsm2 protein expression in a mouse model of IRI-induced AKI. (F) A schematic diagram showing the construction of the FA-induced AKI mouse model. (G,H) Renal function was assessed by measuring serum creatinine (G) and BUN (H) in a mouse model of FA-induced AKI (mice n = 5). (I) Transcriptional level of Acsm2 in FA-induced AKI mouse model (mice n = 5). (J) Western blot analysis of Acsm2 protein expression in a mouse model of FA-induced AKI. (K) A schematic diagram showing the construction of the Cisplatin-induced AKI mouse model. (L,M) Renal function was assessed by measuring serum creatinine (L) and BUN (M) in a mouse model of Cisplatin-induced AKI (mice n = 5). (N) Transcriptional level of Acsm2 in Cisplatin-induced AKI mouse model (mice n = 5). (O) Western blot analysis of Acsm2 protein expression in a mouse model of Cisplatin-induced AKI. (P) Transcriptional level of ACSM2A in OGD/R-induced HK-2 cell models ( n = 3). (Q) Western blot analysis of ACSM2A protein expression in OGD/R-induced HK-2 cell models. (R) Transcriptional level of ACSM2A in OGD/R-induced primary human TECs models ( n = 3). Data are representative of three independent experiments (E, J, O, Q). Error bars are shown as mean ± SD with n = 3 (P, R) or n = 5 (B-D, G-I, L-N) biological replicates. Two-tailed unpaired Student’s t-test (B-D, G-I, L-N, P, R). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.
    Fa 45 24 11, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/Rotor+FA-45-24-11/10__3390_slash_vetsci13100997-82-28-31
    Average 96 stars, based on 1 article reviews
    fa 45 24 11 - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    stereo dissection microscopy  (Danaher Inc)
    96
    Danaher Inc stereo dissection microscopy
    Acsm2/ACSM2A is downregulated in renal tubular <t>epithelial</t> <t>cells</t> from three distinct AKI mouse models and in OGD/R-induced cell models. (A) A schematic diagram showing the construction of the IRI-induced AKI mouse model. (B,C) Renal function was assessed by measuring serum creatinine (B) and BUN (C) in a mouse model of IRI-induced AKI (mice n = 5). (D) Transcriptional level of Acsm2 in IRI-induced AKI mouse model (mice n = 5). (E) Western blot analysis of Acsm2 protein expression in a mouse model of IRI-induced AKI. (F) A schematic diagram showing the construction of the FA-induced AKI mouse model. (G,H) Renal function was assessed by measuring serum creatinine (G) and BUN (H) in a mouse model of FA-induced AKI (mice n = 5). (I) Transcriptional level of Acsm2 in FA-induced AKI mouse model (mice n = 5). (J) Western blot analysis of Acsm2 protein expression in a mouse model of FA-induced AKI. (K) A schematic diagram showing the construction of the Cisplatin-induced AKI mouse model. (L,M) Renal function was assessed by measuring serum creatinine (L) and BUN (M) in a mouse model of Cisplatin-induced AKI (mice n = 5). (N) Transcriptional level of Acsm2 in Cisplatin-induced AKI mouse model (mice n = 5). (O) Western blot analysis of Acsm2 protein expression in a mouse model of Cisplatin-induced AKI. (P) Transcriptional level of ACSM2A in OGD/R-induced HK-2 cell models ( n = 3). (Q) Western blot analysis of ACSM2A protein expression in OGD/R-induced HK-2 cell models. (R) Transcriptional level of ACSM2A in OGD/R-induced primary human TECs models ( n = 3). Data are representative of three independent experiments (E, J, O, Q). Error bars are shown as mean ± SD with n = 3 (P, R) or n = 5 (B-D, G-I, L-N) biological replicates. Two-tailed unpaired Student’s t-test (B-D, G-I, L-N, P, R). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.
    Stereo Dissection Microscopy, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/M205+FA+Fluorescence+Stereo+Microscopes/pm42772574-76-5-8
    Average 96 stars, based on 1 article reviews
    stereo dissection microscopy - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    m205 fa stereo microscope  (Danaher Inc)
    96
    Danaher Inc m205 fa stereo microscope
    Acsm2/ACSM2A is downregulated in renal tubular <t>epithelial</t> <t>cells</t> from three distinct AKI mouse models and in OGD/R-induced cell models. (A) A schematic diagram showing the construction of the IRI-induced AKI mouse model. (B,C) Renal function was assessed by measuring serum creatinine (B) and BUN (C) in a mouse model of IRI-induced AKI (mice n = 5). (D) Transcriptional level of Acsm2 in IRI-induced AKI mouse model (mice n = 5). (E) Western blot analysis of Acsm2 protein expression in a mouse model of IRI-induced AKI. (F) A schematic diagram showing the construction of the FA-induced AKI mouse model. (G,H) Renal function was assessed by measuring serum creatinine (G) and BUN (H) in a mouse model of FA-induced AKI (mice n = 5). (I) Transcriptional level of Acsm2 in FA-induced AKI mouse model (mice n = 5). (J) Western blot analysis of Acsm2 protein expression in a mouse model of FA-induced AKI. (K) A schematic diagram showing the construction of the Cisplatin-induced AKI mouse model. (L,M) Renal function was assessed by measuring serum creatinine (L) and BUN (M) in a mouse model of Cisplatin-induced AKI (mice n = 5). (N) Transcriptional level of Acsm2 in Cisplatin-induced AKI mouse model (mice n = 5). (O) Western blot analysis of Acsm2 protein expression in a mouse model of Cisplatin-induced AKI. (P) Transcriptional level of ACSM2A in OGD/R-induced HK-2 cell models ( n = 3). (Q) Western blot analysis of ACSM2A protein expression in OGD/R-induced HK-2 cell models. (R) Transcriptional level of ACSM2A in OGD/R-induced primary human TECs models ( n = 3). Data are representative of three independent experiments (E, J, O, Q). Error bars are shown as mean ± SD with n = 3 (P, R) or n = 5 (B-D, G-I, L-N) biological replicates. Two-tailed unpaired Student’s t-test (B-D, G-I, L-N, P, R). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.
    M205 Fa Stereo Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/M205+FA+Fluorescence+Stereo+Microscopes/pm42768110-70-17-16
    Average 96 stars, based on 1 article reviews
    m205 fa stereo microscope - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    nefa  (Randox)
    97
    Randox nefa
    Acsm2/ACSM2A is downregulated in renal tubular <t>epithelial</t> <t>cells</t> from three distinct AKI mouse models and in OGD/R-induced cell models. (A) A schematic diagram showing the construction of the IRI-induced AKI mouse model. (B,C) Renal function was assessed by measuring serum creatinine (B) and BUN (C) in a mouse model of IRI-induced AKI (mice n = 5). (D) Transcriptional level of Acsm2 in IRI-induced AKI mouse model (mice n = 5). (E) Western blot analysis of Acsm2 protein expression in a mouse model of IRI-induced AKI. (F) A schematic diagram showing the construction of the FA-induced AKI mouse model. (G,H) Renal function was assessed by measuring serum creatinine (G) and BUN (H) in a mouse model of FA-induced AKI (mice n = 5). (I) Transcriptional level of Acsm2 in FA-induced AKI mouse model (mice n = 5). (J) Western blot analysis of Acsm2 protein expression in a mouse model of FA-induced AKI. (K) A schematic diagram showing the construction of the Cisplatin-induced AKI mouse model. (L,M) Renal function was assessed by measuring serum creatinine (L) and BUN (M) in a mouse model of Cisplatin-induced AKI (mice n = 5). (N) Transcriptional level of Acsm2 in Cisplatin-induced AKI mouse model (mice n = 5). (O) Western blot analysis of Acsm2 protein expression in a mouse model of Cisplatin-induced AKI. (P) Transcriptional level of ACSM2A in OGD/R-induced HK-2 cell models ( n = 3). (Q) Western blot analysis of ACSM2A protein expression in OGD/R-induced HK-2 cell models. (R) Transcriptional level of ACSM2A in OGD/R-induced primary human TECs models ( n = 3). Data are representative of three independent experiments (E, J, O, Q). Error bars are shown as mean ± SD with n = 3 (P, R) or n = 5 (B-D, G-I, L-N) biological replicates. Two-tailed unpaired Student’s t-test (B-D, G-I, L-N, P, R). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.
    Nefa, supplied by Randox, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa/NEFA/custom%40fa%4010%2E3390%2Fnu18183063
    Average 97 stars, based on 1 article reviews
    nefa - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Acsm2/ACSM2A is downregulated in renal tubular epithelial cells from three distinct AKI mouse models and in OGD/R-induced cell models. (A) A schematic diagram showing the construction of the IRI-induced AKI mouse model. (B,C) Renal function was assessed by measuring serum creatinine (B) and BUN (C) in a mouse model of IRI-induced AKI (mice n = 5). (D) Transcriptional level of Acsm2 in IRI-induced AKI mouse model (mice n = 5). (E) Western blot analysis of Acsm2 protein expression in a mouse model of IRI-induced AKI. (F) A schematic diagram showing the construction of the FA-induced AKI mouse model. (G,H) Renal function was assessed by measuring serum creatinine (G) and BUN (H) in a mouse model of FA-induced AKI (mice n = 5). (I) Transcriptional level of Acsm2 in FA-induced AKI mouse model (mice n = 5). (J) Western blot analysis of Acsm2 protein expression in a mouse model of FA-induced AKI. (K) A schematic diagram showing the construction of the Cisplatin-induced AKI mouse model. (L,M) Renal function was assessed by measuring serum creatinine (L) and BUN (M) in a mouse model of Cisplatin-induced AKI (mice n = 5). (N) Transcriptional level of Acsm2 in Cisplatin-induced AKI mouse model (mice n = 5). (O) Western blot analysis of Acsm2 protein expression in a mouse model of Cisplatin-induced AKI. (P) Transcriptional level of ACSM2A in OGD/R-induced HK-2 cell models ( n = 3). (Q) Western blot analysis of ACSM2A protein expression in OGD/R-induced HK-2 cell models. (R) Transcriptional level of ACSM2A in OGD/R-induced primary human TECs models ( n = 3). Data are representative of three independent experiments (E, J, O, Q). Error bars are shown as mean ± SD with n = 3 (P, R) or n = 5 (B-D, G-I, L-N) biological replicates. Two-tailed unpaired Student’s t-test (B-D, G-I, L-N, P, R). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Multi-omics integration identifies Acsm2/ACSM2A deficiency as a metabolic driver of ZBP1-mediated PANoptosis in acute kidney injury

    doi: 10.3389/fcell.2026.1936504

    Figure Lengend Snippet: Acsm2/ACSM2A is downregulated in renal tubular epithelial cells from three distinct AKI mouse models and in OGD/R-induced cell models. (A) A schematic diagram showing the construction of the IRI-induced AKI mouse model. (B,C) Renal function was assessed by measuring serum creatinine (B) and BUN (C) in a mouse model of IRI-induced AKI (mice n = 5). (D) Transcriptional level of Acsm2 in IRI-induced AKI mouse model (mice n = 5). (E) Western blot analysis of Acsm2 protein expression in a mouse model of IRI-induced AKI. (F) A schematic diagram showing the construction of the FA-induced AKI mouse model. (G,H) Renal function was assessed by measuring serum creatinine (G) and BUN (H) in a mouse model of FA-induced AKI (mice n = 5). (I) Transcriptional level of Acsm2 in FA-induced AKI mouse model (mice n = 5). (J) Western blot analysis of Acsm2 protein expression in a mouse model of FA-induced AKI. (K) A schematic diagram showing the construction of the Cisplatin-induced AKI mouse model. (L,M) Renal function was assessed by measuring serum creatinine (L) and BUN (M) in a mouse model of Cisplatin-induced AKI (mice n = 5). (N) Transcriptional level of Acsm2 in Cisplatin-induced AKI mouse model (mice n = 5). (O) Western blot analysis of Acsm2 protein expression in a mouse model of Cisplatin-induced AKI. (P) Transcriptional level of ACSM2A in OGD/R-induced HK-2 cell models ( n = 3). (Q) Western blot analysis of ACSM2A protein expression in OGD/R-induced HK-2 cell models. (R) Transcriptional level of ACSM2A in OGD/R-induced primary human TECs models ( n = 3). Data are representative of three independent experiments (E, J, O, Q). Error bars are shown as mean ± SD with n = 3 (P, R) or n = 5 (B-D, G-I, L-N) biological replicates. Two-tailed unpaired Student’s t-test (B-D, G-I, L-N, P, R). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.

    Article Snippet: To establish the folic acid (FA)- or cisplatin-induced acute kidney injury (AKI) model, mice received a single intraperitoneal injection of FA (200 mg/kg; MCE, HY-16637) or cisplatin (10 mg/kg; MCE, HY-17394).

    Techniques: Western Blot, Analysis, Expressing, Two Tailed Test

    ACSM2A downregulation promotes PANoptosis in renal tubular epithelial cells during AKI. (A) LDH release from control and OGD/R-treated HK-2 cells as a measure of cytotoxicity ( n = 6). (B) Cell death quantification in control and OGD/R-treated HK-2 cells ( n = 6). (C) Western blot analysis of Caspase 3, Cleaved-caspase 3 (c-caspase3), MLKL, Phosphorylated-MLKL (p-MLKL), GSDMD and Cleaved-GSDMD (c-GSDMD) protein expression in control and OGD/R-treated HK-2 cells. (D) Cell death quantification in control and OGD/R-treated HK-2 cells, pre-treated with dimethylsulfoxide (DMSO; control), 20 µM Z-VAD-FMK, 10 µM Disulfiram, 10 µM GSK-872 or a combination of all three inhibitors for 1 h ( n = 3). (E) Cytotoxicity assessed by LDH release from HK-2 cells following siRNA-mediated knockdown of ACSM2A for 48 h ( n = 3). (F) Cell death quantification in HK-2 cells following siRNA-mediated knockdown of ACSM2A for 48 h ( n = 3). (G) Cell death quantification in HK-2 cells transfected with ACSM2A siRNA for 24 h, followed by treatment with DMSO (control), 20 µM Z-VAD-FMK, 10 µM disulfiram, 10 µM GSK-872, or a combination of all three inhibitors for an additional 24 h ( n = 3). (H) Cell death quantification in HK-2 cells transfected with ACSM2A siRNA for 48 h and subsequently subjected to control or OGD/R treatment ( n = 3). (I) Cell death quantification in HK-2 cells overexpressing of ACSM2A for 48 h and subsequently subjected to control or OGD/R treatment ( n = 3). (J) Western blot analysis of ACSM2A and GAPDH were conducted in control HK-2 cells, cells subjected to ACSM2A knockdown, and cells in which ACSM2A was overexpressed subsequent to ACSM2A knockdown. (K) Cell death quantification in HK-2 cells under the indicated treatment conditions, as shown in Fig. (J) ( n = 3). (L) Western blot analysis of Caspase 3, Cleaved-caspase 3 (c-caspase3), MLKL, Phosphorylated-MLKL (p-MLKL), GSDMD and Cleaved-GSDMD (c-GSDMD) protein expression in HK-2 cells under the indicated treatment conditions, as shown in Fig. (J) . (M) Cell death quantification in primary human TECs under the indicated treatment conditions, as shown in Fig. J ( n = 3). Data are representative of three independent experiments (C, J, L) . Error bars are shown as mean ± SD with n = 3 (D-I, K, M) or n = 6 (A,B) biological replicates. Two-tailed unpaired Student’s t-test (A,B,E,F) . One-way ANOVA with Tukey’s multiple comparisons test (D, G, H, I, K, M) . * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Multi-omics integration identifies Acsm2/ACSM2A deficiency as a metabolic driver of ZBP1-mediated PANoptosis in acute kidney injury

    doi: 10.3389/fcell.2026.1936504

    Figure Lengend Snippet: ACSM2A downregulation promotes PANoptosis in renal tubular epithelial cells during AKI. (A) LDH release from control and OGD/R-treated HK-2 cells as a measure of cytotoxicity ( n = 6). (B) Cell death quantification in control and OGD/R-treated HK-2 cells ( n = 6). (C) Western blot analysis of Caspase 3, Cleaved-caspase 3 (c-caspase3), MLKL, Phosphorylated-MLKL (p-MLKL), GSDMD and Cleaved-GSDMD (c-GSDMD) protein expression in control and OGD/R-treated HK-2 cells. (D) Cell death quantification in control and OGD/R-treated HK-2 cells, pre-treated with dimethylsulfoxide (DMSO; control), 20 µM Z-VAD-FMK, 10 µM Disulfiram, 10 µM GSK-872 or a combination of all three inhibitors for 1 h ( n = 3). (E) Cytotoxicity assessed by LDH release from HK-2 cells following siRNA-mediated knockdown of ACSM2A for 48 h ( n = 3). (F) Cell death quantification in HK-2 cells following siRNA-mediated knockdown of ACSM2A for 48 h ( n = 3). (G) Cell death quantification in HK-2 cells transfected with ACSM2A siRNA for 24 h, followed by treatment with DMSO (control), 20 µM Z-VAD-FMK, 10 µM disulfiram, 10 µM GSK-872, or a combination of all three inhibitors for an additional 24 h ( n = 3). (H) Cell death quantification in HK-2 cells transfected with ACSM2A siRNA for 48 h and subsequently subjected to control or OGD/R treatment ( n = 3). (I) Cell death quantification in HK-2 cells overexpressing of ACSM2A for 48 h and subsequently subjected to control or OGD/R treatment ( n = 3). (J) Western blot analysis of ACSM2A and GAPDH were conducted in control HK-2 cells, cells subjected to ACSM2A knockdown, and cells in which ACSM2A was overexpressed subsequent to ACSM2A knockdown. (K) Cell death quantification in HK-2 cells under the indicated treatment conditions, as shown in Fig. (J) ( n = 3). (L) Western blot analysis of Caspase 3, Cleaved-caspase 3 (c-caspase3), MLKL, Phosphorylated-MLKL (p-MLKL), GSDMD and Cleaved-GSDMD (c-GSDMD) protein expression in HK-2 cells under the indicated treatment conditions, as shown in Fig. (J) . (M) Cell death quantification in primary human TECs under the indicated treatment conditions, as shown in Fig. J ( n = 3). Data are representative of three independent experiments (C, J, L) . Error bars are shown as mean ± SD with n = 3 (D-I, K, M) or n = 6 (A,B) biological replicates. Two-tailed unpaired Student’s t-test (A,B,E,F) . One-way ANOVA with Tukey’s multiple comparisons test (D, G, H, I, K, M) . * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.

    Article Snippet: To establish the folic acid (FA)- or cisplatin-induced acute kidney injury (AKI) model, mice received a single intraperitoneal injection of FA (200 mg/kg; MCE, HY-16637) or cisplatin (10 mg/kg; MCE, HY-17394).

    Techniques: Control, Western Blot, Analysis, Expressing, Knockdown, Transfection, Two Tailed Test

    ACSM2A deficiency in renal tubular epithelial cells promotes ZBP1-mediated PANoptosis by inducing mitochondrial oxidative stress and Z-nucleic acid accumulation. (A) Intracellular ATP levels in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells with ACSM2A overexpression. (B,C) Representative fluorescence images (B) and quantitative analysis (C) of MitoSox in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells with ACSM2A overexpression. Red fluorescence indicates MitoSox (mitochondrial superoxide). (D,E) Representative fluorescence images (D) and quantitative analysis (E) of DCFH-DA in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells with ACSM2A overexpression. Green fluorescence indicates DCFH-DA (ROS). (F,G) Representative fluorescence images (F) and quantification (G) of Z22 levels in control HK-2 cells, ACSM2A-knockdown cells, ACSM2A-knockdown cells with ACSM2A overexpression, ACSM2A-knockdown cells treated with mito-TEMPO (10 μM) for 24 h and control HK-2 cells treated with MitoPQ (10 μM) for 12 h. Green fluorescence indicates Z22 (Z-NA). Blue fluorescence indicates DAPI (nucleus). (H) Cell death quantification in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells treated with mito-TEMPO (10 μM) for 24 h ( n = 3). (I) Cell death quantification in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells with ZBP1 knockdown ( n = 3). Data are representative of three independent experiments (B,D,F) . Error bars are shown as mean ± SD with n = 3 (A,C,E,H,I) or n = 6 (G) biological replicates. One-way ANOVA with Tukey’s multiple comparisons test (A,C,E,G,H,I) . * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Multi-omics integration identifies Acsm2/ACSM2A deficiency as a metabolic driver of ZBP1-mediated PANoptosis in acute kidney injury

    doi: 10.3389/fcell.2026.1936504

    Figure Lengend Snippet: ACSM2A deficiency in renal tubular epithelial cells promotes ZBP1-mediated PANoptosis by inducing mitochondrial oxidative stress and Z-nucleic acid accumulation. (A) Intracellular ATP levels in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells with ACSM2A overexpression. (B,C) Representative fluorescence images (B) and quantitative analysis (C) of MitoSox in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells with ACSM2A overexpression. Red fluorescence indicates MitoSox (mitochondrial superoxide). (D,E) Representative fluorescence images (D) and quantitative analysis (E) of DCFH-DA in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells with ACSM2A overexpression. Green fluorescence indicates DCFH-DA (ROS). (F,G) Representative fluorescence images (F) and quantification (G) of Z22 levels in control HK-2 cells, ACSM2A-knockdown cells, ACSM2A-knockdown cells with ACSM2A overexpression, ACSM2A-knockdown cells treated with mito-TEMPO (10 μM) for 24 h and control HK-2 cells treated with MitoPQ (10 μM) for 12 h. Green fluorescence indicates Z22 (Z-NA). Blue fluorescence indicates DAPI (nucleus). (H) Cell death quantification in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells treated with mito-TEMPO (10 μM) for 24 h ( n = 3). (I) Cell death quantification in control HK-2 cells, ACSM2A-knockdown cells, and ACSM2A-knockdown cells with ZBP1 knockdown ( n = 3). Data are representative of three independent experiments (B,D,F) . Error bars are shown as mean ± SD with n = 3 (A,C,E,H,I) or n = 6 (G) biological replicates. One-way ANOVA with Tukey’s multiple comparisons test (A,C,E,G,H,I) . * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, not significant.

    Article Snippet: To establish the folic acid (FA)- or cisplatin-induced acute kidney injury (AKI) model, mice received a single intraperitoneal injection of FA (200 mg/kg; MCE, HY-16637) or cisplatin (10 mg/kg; MCE, HY-17394).

    Techniques: Control, Knockdown, Over Expression, Fluorescence, Analysis