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Nacalai dmem f 12 medium
Dmem F 12 Medium, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/f+12/dmem/pm42283489-175-5-7
Average 86 stars, based on 1 article reviews
dmem f 12 medium - by Bioz Stars, 2026-10
86/100 stars

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other:

Article Title: Non-nutritive sweeteners attenuate reinforced cephalic phase insulin release and impair glucose tolerance in mice
Article Snippet: The test solutions included D -glucose (Nacalai Tesque, 16806–25), saccharin sodium dihydrate (Sigma-Aldrich, S6047), sucralose (Tokyo Chemical Industry, S0839), and acesulfame potassium (Tokyo Chemical Industry, A1490) at various concentrations.

Cell Culture:

Article Title: Activation-induced cytidine deaminase promotes DNA demethylation and expression of the Ninjurin-2 gene.
Article Snippet: LoVo cells were cultured in Ham’s F-12 medium (Nacalai Tesque) supplemented with 10% heatinactivated FBS (Sigma) and 25 mM HEPES (Nacalai Tesque). .. T84 cells were cultured in DMEM/F-12 medium (Nacalai Tesque) supplemented with 5% heat-inactivated FBS (Sigma). .. We generated cloning primers (Table S1) based on the human AID coding sequence in the Ensembl Genome Browser (GRCh37) and used them for plasmid insertion.

Article Title: Activation-induced cytidine deaminase promotes DNA demethylation and expression of the Ninjurin-2 gene.
Article Snippet: Caco-2 cells were cultured in Eagle’s minimum essential medium (MEM; Nacalai Tesque, Kyoto, Japan) supplemented with 20% heat-inactivated FBS (Sigma), 0.1 mM MEM nonessential amino acid (NEAA) solution (Nacalai Tesque), and 1 mM sodium pyruvate solution (Nacalai Tesque). .. LoVo cells were cultured in Ham’s F-12 medium (Nacalai Tesque) supplemented with 10% heatinactivated FBS (Sigma) and 25 mM HEPES (Nacalai Tesque). .. T84 cells were cultured in DMEM/F-12 medium (Nacalai Tesque) supplemented with 5% heat-inactivated FBS (Sigma).

Article Title: Effect of terahertz irradiation on DNA damage repair in living cells.
Article Snippet: .. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Waltham, MA, USA) and 100 U/mL penicillin–streptomycin (Thermo Fisher Scientific) at 37 °C in a 5% CO2 humidified atmosphere. .. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Waltham, MA, USA) and 100 U/mL penicillin–streptomycin (Thermo Fisher Scientific) at 37 °C in a 5% CO2 humidified atmosphere.

Article Title: Non-invasive quantitative assessment of kidney injury using near-infrared autofluorescence imaging.
Article Snippet: .. Slices were cultured at 37°C in highglucose DMEM (16971-55, Nacalai Tesque, Kyoto, Japan) containing 10% fetal calf serum Jo urn al Pr e-p roo f 13 and penicillin-streptomycin (15140122, Gibco), with 1.5 ml of medium per well of a 6-well plate. ..

Article Title: Ceramide kinase/ceramide 1-phosphate signaling regulates LC3B expression and autophagosome formation.
Article Snippet: .. WT and CERK-KO cells were routinely cultured in DMEM (Nacalai Tesque, Kyoto, Japan) supplemented with 10% FBS and 1% antibiotics. .. For transient knockdown of CERK, WT HeLa cells were transfected with control siRNA (siCtrl) or siRNA targeting CERK (siCERK) using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s instructions.

Modification:

Article Title: Ion transport peptide regulates larval body water balance via a receptor guanylyl cyclase in Drosophila
Article Snippet: S2 cells obtained from Tadashi Uemura (Kyoto University) were maintained in 25-cm 2 cell-culture flasks (NEST #707003) in a humidified incubator at 25°C in Schneider’s Drosophila medium (SDM; Thermo Fisher Gibco #21720024) containing 10% fetal bovine serum (FBS; Thermo Fisher Gibco #10270-106) and 1% Penicillin-streptomycin solution (P/S; Fujifilm Wako #168-23191). .. HEK293T cells obtained from Hiroaki Daitoku (University of Tsukuba) were maintained in Dulbecco's Modified Eagle Medium (DMEM; Nacalai Tesque #08456-36) containing 10% FBS (Gibco #F7524) and 1% P/S (Nacalai Tesque #09367-34) in 100-mm Type-I-collagen-coated dishes (Iwaki #4020-010-MYP) in a humidified incubator at 37°C under 5% CO 2 . .. Before seeding the cells on new plates, they were washed with Dulbecco's phosphate-buffered saline (D-PBS; Nacalai Tesque #11482-15) and treated with 0.02% EDTA (Nacalai Tesque #15130-95).

Article Title: Effect of terahertz irradiation on DNA damage repair in living cells.
Article Snippet: .. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Waltham, MA, USA) and 100 U/mL penicillin–streptomycin (Thermo Fisher Scientific) at 37 °C in a 5% CO2 humidified atmosphere. .. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Waltham, MA, USA) and 100 U/mL penicillin–streptomycin (Thermo Fisher Scientific) at 37 °C in a 5% CO2 humidified atmosphere.

Article Title: Mesenchymal stem cell-mediated delivery boosts the efficacy of suicide gene therapy based on retroviral replicating vectors in peritoneally disseminated cancer.
Article Snippet: Normal human adult mesothelial cells (NMCs) were obtained from Zen-Bio, Inc. (Research Triangle Park, NC, USA) and cultured in mesothelial cell growth medium (Zen-Bio, Inc.). .. Human embryonic kidney 293T cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 10% FBS. ..



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