Extraction:
Solvent:
High Performance Liquid Chromatography:Article Title: Optimization of ultrasound-assisted enzymatic extraction of saponins from Panax zingiberensis stems and leaves.
Article Snippet: .. Ultrasound-assisted extractor (KQ-500DE, Kunshan Ultrasonic Instrument Co., Ltd., China ), High-performance liquid chromatography (HPLC) (Agilent 1260 Infinity II, Agilent Technologies, USA); Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS); (Waters Xevo G2-XS QTof, Waters Corporation, USA.); pH meter (PB-10, Sartorius AG, Germany). .. The ginsenoside Rg1 reference standard was obtained from Yunnan Yingge Biotechnology Co., Ltd. Cellulase (11 U/mg) and compound pectinase (60 U/mg) were supplied by Changzhou Xiasheng Enzyme Biotechnology Co., Ltd. Vanillin was purchased from Yuanye Biotechnology Co., Ltd.; glacial acetic acid was from Chongqing Chuandong Chemical Co., Ltd.; perchloric acid was from City Cologne Chemical Co., Ltd.; and absolute ethanol was from Tianjin Zhiyuan Chemical Reagent Co., Ltd. Methanol and acetonitrile were supplied by Merck, and formic acid was sourced from Aladdin.
Article Title: Optimization of ultrasound-assisted enzymatic extraction of saponins from Panax zingiberensis stems and leaves
Article Snippet: .. Ultrasound-assisted extractor (KQ-500DE, Kunshan Ultrasonic Instrument Co., Ltd., China ), High-performance liquid chromatography (HPLC) (Agilent 1260 Infinity II, Agilent Technologies, USA); Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS); (Waters Xevo G2-XS QTof, Waters Corporation, USA.); pH meter (PB-10, Sartorius AG, Germany). .. The ginsenoside Rg1 reference standard was obtained from Yunnan Yingge Biotechnology Co., Ltd. Cellulase (11 U/mg) and compound pectinase (60 U/mg) were supplied by Changzhou Xiasheng Enzyme Biotechnology Co., Ltd. Vanillin was purchased from Yuanye Biotechnology Co., Ltd.; glacial acetic acid was from Chongqing Chuandong Chemical Co., Ltd.; perchloric acid was from City Cologne Chemical Co., Ltd.; and absolute ethanol was from Tianjin Zhiyuan Chemical Reagent Co., Ltd. Methanol and acetonitrile were supplied by Merck, and formic acid was sourced from Aladdin.
Chromatography:Article Title: Optimization of ultrasound-assisted enzymatic extraction of saponins from Panax zingiberensis stems and leaves.
Article Snippet: .. Ultrasound-assisted extractor (KQ-500DE, Kunshan Ultrasonic Instrument Co., Ltd., China ), High-performance liquid chromatography (HPLC) (Agilent 1260 Infinity II, Agilent Technologies, USA); Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS); (Waters Xevo G2-XS QTof, Waters Corporation, USA.); pH meter (PB-10, Sartorius AG, Germany). .. The ginsenoside Rg1 reference standard was obtained from Yunnan Yingge Biotechnology Co., Ltd. Cellulase (11 U/mg) and compound pectinase (60 U/mg) were supplied by Changzhou Xiasheng Enzyme Biotechnology Co., Ltd. Vanillin was purchased from Yuanye Biotechnology Co., Ltd.; glacial acetic acid was from Chongqing Chuandong Chemical Co., Ltd.; perchloric acid was from City Cologne Chemical Co., Ltd.; and absolute ethanol was from Tianjin Zhiyuan Chemical Reagent Co., Ltd. Methanol and acetonitrile were supplied by Merck, and formic acid was sourced from Aladdin.
Article Title: Optimization of ultrasound-assisted enzymatic extraction of saponins from Panax zingiberensis stems and leaves
Article Snippet: .. Ultrasound-assisted extractor (KQ-500DE, Kunshan Ultrasonic Instrument Co., Ltd., China ), High-performance liquid chromatography (HPLC) (Agilent 1260 Infinity II, Agilent Technologies, USA); Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS); (Waters Xevo G2-XS QTof, Waters Corporation, USA.); pH meter (PB-10, Sartorius AG, Germany). .. The ginsenoside Rg1 reference standard was obtained from Yunnan Yingge Biotechnology Co., Ltd. Cellulase (11 U/mg) and compound pectinase (60 U/mg) were supplied by Changzhou Xiasheng Enzyme Biotechnology Co., Ltd. Vanillin was purchased from Yuanye Biotechnology Co., Ltd.; glacial acetic acid was from Chongqing Chuandong Chemical Co., Ltd.; perchloric acid was from City Cologne Chemical Co., Ltd.; and absolute ethanol was from Tianjin Zhiyuan Chemical Reagent Co., Ltd. Methanol and acetonitrile were supplied by Merck, and formic acid was sourced from Aladdin.
Mass Spectrometry:Article Title: Optimization of ultrasound-assisted enzymatic extraction of saponins from Panax zingiberensis stems and leaves.
Article Snippet: .. Ultrasound-assisted extractor (KQ-500DE, Kunshan Ultrasonic Instrument Co., Ltd., China ), High-performance liquid chromatography (HPLC) (Agilent 1260 Infinity II, Agilent Technologies, USA); Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS); (Waters Xevo G2-XS QTof, Waters Corporation, USA.); pH meter (PB-10, Sartorius AG, Germany). .. The ginsenoside Rg1 reference standard was obtained from Yunnan Yingge Biotechnology Co., Ltd. Cellulase (11 U/mg) and compound pectinase (60 U/mg) were supplied by Changzhou Xiasheng Enzyme Biotechnology Co., Ltd. Vanillin was purchased from Yuanye Biotechnology Co., Ltd.; glacial acetic acid was from Chongqing Chuandong Chemical Co., Ltd.; perchloric acid was from City Cologne Chemical Co., Ltd.; and absolute ethanol was from Tianjin Zhiyuan Chemical Reagent Co., Ltd. Methanol and acetonitrile were supplied by Merck, and formic acid was sourced from Aladdin.
Article Title: Optimization of ultrasound-assisted enzymatic extraction of saponins from Panax zingiberensis stems and leaves
Article Snippet: .. Ultrasound-assisted extractor (KQ-500DE, Kunshan Ultrasonic Instrument Co., Ltd., China ), High-performance liquid chromatography (HPLC) (Agilent 1260 Infinity II, Agilent Technologies, USA); Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS); (Waters Xevo G2-XS QTof, Waters Corporation, USA.); pH meter (PB-10, Sartorius AG, Germany). .. The ginsenoside Rg1 reference standard was obtained from Yunnan Yingge Biotechnology Co., Ltd. Cellulase (11 U/mg) and compound pectinase (60 U/mg) were supplied by Changzhou Xiasheng Enzyme Biotechnology Co., Ltd. Vanillin was purchased from Yuanye Biotechnology Co., Ltd.; glacial acetic acid was from Chongqing Chuandong Chemical Co., Ltd.; perchloric acid was from City Cologne Chemical Co., Ltd.; and absolute ethanol was from Tianjin Zhiyuan Chemical Reagent Co., Ltd. Methanol and acetonitrile were supplied by Merck, and formic acid was sourced from Aladdin.
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