spin-column-based extraction method (Qiagen)

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Qiagen spin-column-based extraction method
Spin Column Based Extraction Method, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/extraction+method+column+based+extraction+method/spin+columns/pmc10742398-84-2-4
Average 90 stars, based on 1 article reviews

spin-column-based extraction method - by Bioz Stars, 2026-10

90/100 stars

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Binding Assay:

Article Title: A Comparative Longitudinal Study Analyzing Vaginal Microbiota Differences Between Term and Preterm Pregnancies in Korean Women
Article Snippet: The extraction procedure included the addition of 20 μL of Proteinase K (20 mg/mL) and 400 μL of an AL buffer (Qiagen) to 370 μL of the sample suspension, followed by mixing for 15 s and incubation at 56 °C for 10 min to ensure complete lysis while preserving DNA integrity. .. Subsequently, 400 μL of ethanol (96–100%) was added, and the mixture was carefully transferred to Qiagen spin columns to allow for high-purity DNA binding and purification. .. The elution step was carried out using 100 μL of an AE buffer (Qiagen), and the extracted DNA was immediately quantified using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).

Purification:

Article Title: Replicating and non-replicating vectors for recombinant protein production in plants and method of use thereof
Article Snippet: Prior to circularization, 5 μg total RNA was decapped with 25 U of RNA 5′ pyrophosphohydrolase (NEB) and 40 U of RNAseOUT RNA inhibitor (Invitrogen) in a total volume of 50 μl buffer (20 mM Tris-HCl, pH 8.8, 10 mM (NH₄)₂SO₄, 10 mM KCl, 2 mM MgSO₄, 0.1% Triton X-100) for 1 hour at 37° C. The reaction was stopped by addition of 1 μl of 500 mM EDTA, and heated to 65° C. for 5 minutes. .. Decapped RNA was purified using a spin column (Qiagen®). .. RNA was then circularized with 10 U of T4 RNA Ligase (NEB) and 40 U RNAseOUT in a total volume of 20 μl buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl₂, 1 mM DTT, 10% PEG8000, 50 μM ATP) for 1.5 hours at 25° C. The reaction was terminated by boiling for 2 minutes.

Article Title:
Article Snippet: First, the idp genes were PCR amplified from idp-fret constructs using Q5 Polymerase (NEB) and the primers listed in SI Table S1. .. The two PCR products were purified on a spin column (Qiagen) and quantified by A260. .. The two PCR products were purified on a spin column (Qiagen) and quantified by A260.

Article Title:
Article Snippet: .. The resulting DNA fragments were purified on a spin column (Qiagen) and quantified by A260. .. The resulting DNA fragments were purified on a spin column (Qiagen) and quantified by A260.

Polymerase Chain Reaction:

DNA Extraction:

Article Title: Seasonality and dynamics of schistosomiasis in the environment: usefulness of environmental DNA (eDNA) surveillance system at a community level for risk mapping schistosomiasis in Ekiran Village, Leyte, Philippines.
Article Snippet: Post-incubation, the filter was attached to a salivette tube (Sarstedt AG & Co. KG, Nümbrecht, Germany) and centrifuged for 3 minutes at 3,000 × g. Refrigerated absolute ethanol was added to the lysed filtrate at half the volume collected so that the final concentration of ethanol was one-third of the total volume and incubated in refrigeration for at least an hour, preferably overnight. .. Afterward, the precipitate was transferred to a spin column (QIAGEN), and the DNA extraction was completed by following the manufacturer’s protocol with a final elution performed twice with 55 μL of elution buffer each time, resulting in a total volume of 110 μL eluate. ..

Extraction:

Article Title: MB based RT-qPCR increase the clinical application of cfEBV DNA for NPC in non-endemic area of China.
Article Snippet: .. Many previous endemic studies utilized nucleic acid extraction through spin column (Qiagen). ..

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