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mitochondrial extraction kit  (Beijing Solarbio Science)


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    Beijing Solarbio Science mitochondrial extraction kit
    Mitochondrial Extraction Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/extraction+kit/mitochondrial+extraction+kit/pm41431176-258-13-17
    Average 96 stars, based on 548 article reviews
    mitochondrial extraction kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Extraction:

    Article Title: Silver nanoparticles induce binding of mitochondrial DNA polymerase gamma subunit Polg2 and mitochondrial dysfunction in HeLa cells.
    Article Snippet: the biosafety of engineered nanoparticles, with particular focus on AgNPs (Sahani and Sharma 2021, Monopoli et al. 2012, Kaushal et al. 2023, Azevedo et al. 2024).. Currently employed in food packaging and medicinal uses, AgNPs exhibit dose-dependent cytotoxicity mediated through multiple molecular pathways (Choi et al. 2008).. Emerging evidence suggests that oxidative stress represents an early hallmark of AgNP-induced cellular damage.

    Article Title: RIPC Protects Against Renal Ischaemia–Reperfusion Injury by Activating SIRT 3/Parkin‐Mediated Mitophagy
    Article Snippet: Chloroquine (CQ; Sigma‐Aldrich), a late‐stage autophagy inhibitor that blocks autophagosome‐lysosome fusion, was dissolved in sterile PBS to a stock concentration of 100 mM and used at a final concentration of 50 μM for in vitro cell experiments [ , ]. .. Mitochondria were isolated from renal tissues using a mitochondrial extraction kit (EX1321; Solarbio) according to the instructions. ..

    Article Title: Targeting PDCD4 rescues sepsis-induced liver injury by rebalancing mitochondrial quality control via YME1L1 degradation
    Article Snippet: .. 8 Cell mitochondrial isolation 9 Mitochondrial extraction was performed using a mitochondria isolation kit (SM0020, Solarbio) and a 10 Dounce homogenizer (2 mL capacity). ..

    Article Title: The Drp1-CoQ10-Coa6-ETC axis represents a therapeutic potential for working memory impairment caused by neuronal mitochondrial dysfunction
    Article Snippet: .. Mitochondria isolation was performed using the mitochondrial extraction kit according to the instructions (SM0020, Solarbio, Beijing, China). ..

    Article Title: SIRT5 Attenuates Traumatic Brain Injury by Preserving OPA1-Mediated Mitochondrial Fusion and Limiting Oxidative Stress.
    Article Snippet: Mitochondrial isolation and respiratory chain complex activity assays The activities of mitochondrial respiratory chain complexes I–V were assessed using commercial assay kits (Solarbio, BC0515 for complex I, BC3230 for complex II, BC3240 for complex III, BC0945 for complex IV, and BC1445 for complex V) according to the manufacturer’s instructions. .. Mitochondria were first isolated from fresh tissue or cells using a mitochondrial extraction kit (Solarbio, BC3270). ..

    Article Title: Mitochondrial homeostasis regulation in the prefrontal cortex: A key mechanism for emodin-mediated attenuation of insomnia.
    Article Snippet: Ethnopharmacological relevance: Insomnia represents a prevalent sleep disorder with neurobiological underpinnings that are not yet fully elucidated.. Emerging research indicates that mitochondrial homeostasis plays a pivotal role in the regulation of sleep.. Emodin (EMD), the principal bioactive constituent of the traditional Chinese medicinal herb Polygonum multiflorum, has been reported to exert antioxidant properties and promote sleep.

    Isolation:

    Article Title: RIPC Protects Against Renal Ischaemia–Reperfusion Injury by Activating SIRT 3/Parkin‐Mediated Mitophagy
    Article Snippet: Chloroquine (CQ; Sigma‐Aldrich), a late‐stage autophagy inhibitor that blocks autophagosome‐lysosome fusion, was dissolved in sterile PBS to a stock concentration of 100 mM and used at a final concentration of 50 μM for in vitro cell experiments [ , ]. .. Mitochondria were isolated from renal tissues using a mitochondrial extraction kit (EX1321; Solarbio) according to the instructions. ..

    Article Title: Targeting PDCD4 rescues sepsis-induced liver injury by rebalancing mitochondrial quality control via YME1L1 degradation
    Article Snippet: .. 8 Cell mitochondrial isolation 9 Mitochondrial extraction was performed using a mitochondria isolation kit (SM0020, Solarbio) and a 10 Dounce homogenizer (2 mL capacity). ..

    Article Title: The Drp1-CoQ10-Coa6-ETC axis represents a therapeutic potential for working memory impairment caused by neuronal mitochondrial dysfunction
    Article Snippet: .. Mitochondria isolation was performed using the mitochondrial extraction kit according to the instructions (SM0020, Solarbio, Beijing, China). ..

    Article Title: SIRT5 Attenuates Traumatic Brain Injury by Preserving OPA1-Mediated Mitochondrial Fusion and Limiting Oxidative Stress.
    Article Snippet: Mitochondrial isolation and respiratory chain complex activity assays The activities of mitochondrial respiratory chain complexes I–V were assessed using commercial assay kits (Solarbio, BC0515 for complex I, BC3230 for complex II, BC3240 for complex III, BC0945 for complex IV, and BC1445 for complex V) according to the manufacturer’s instructions. .. Mitochondria were first isolated from fresh tissue or cells using a mitochondrial extraction kit (Solarbio, BC3270). ..

    Article Title: Dual functionalization of steviol enables mitochondrial targeting and redox modulation in antitumor therapy.
    Article Snippet: Mitochondria are essential for cancer cell survival, with the thioredoxin/thioredoxin reductase 2 (Trx/TrxR2) system acting as a key redox regulator.. Steviol, an abundant natural ent-kaurane diterpenoid, exhibits negligible cytotoxicity, while most active ent-kaurane analogs depend on a reactive exo-methylene cyclopentanone moiety, raising selectivity and safety concerns.. To address these limitations, 28 triphenylphosphonium (TPP)-conjugated steviol derivatives were synthesized to enhance mitochondrial accumulation and modulate mitochondrial signaling.

    Article Title: Mitochondrial homeostasis regulation in the prefrontal cortex: A key mechanism for emodin-mediated attenuation of insomnia.
    Article Snippet: Ethnopharmacological relevance: Insomnia represents a prevalent sleep disorder with neurobiological underpinnings that are not yet fully elucidated.. Emerging research indicates that mitochondrial homeostasis plays a pivotal role in the regulation of sleep.. Emodin (EMD), the principal bioactive constituent of the traditional Chinese medicinal herb Polygonum multiflorum, has been reported to exert antioxidant properties and promote sleep.

    Article Title: Mitochondrial ‘Attack-and-Trap’ strategy potentiates cGAS-STING signaling to stimulate durable antitumor immunity against glioblastoma
    Article Snippet: Materials: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), cholesterol, DSPE-TK-PEG2000, Mdivi-1, 1,3-Diphenylisobenzofuran (DPBF), 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine,4chlorobenzenesulfonate Salt (DiD), 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO), and MitoSOX Red were purchased from Medchemexpress (Shanghai, China). .. Chlorin (Ce6), Mitochondria Isolation Kit, Cell Counting Kit-8, MitoTracker Green, 4’,6-diamidino-2phenylindole (DAPI), and JC-1 Mitochondrial Membrane Potential Assay Kit was obtained from Solarbio LIFE SCIENCES (Beijing, China). .. CellROX was purchased from Thermo Fisher Scientific (Massachusetts, USA).

    Tissue:

    Article Title: SIRT5 Attenuates Traumatic Brain Injury by Preserving OPA1-Mediated Mitochondrial Fusion and Limiting Oxidative Stress.
    Article Snippet: Mitochondrial isolation and respiratory chain complex activity assays The activities of mitochondrial respiratory chain complexes I–V were assessed using commercial assay kits (Solarbio, BC0515 for complex I, BC3230 for complex II, BC3240 for complex III, BC0945 for complex IV, and BC1445 for complex V) according to the manufacturer’s instructions. .. Mitochondria were first isolated from fresh tissue or cells using a mitochondrial extraction kit (Solarbio, BC3270). ..

    CCK-8 Assay:

    Article Title: Mitochondrial ‘Attack-and-Trap’ strategy potentiates cGAS-STING signaling to stimulate durable antitumor immunity against glioblastoma
    Article Snippet: Materials: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), cholesterol, DSPE-TK-PEG2000, Mdivi-1, 1,3-Diphenylisobenzofuran (DPBF), 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine,4chlorobenzenesulfonate Salt (DiD), 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO), and MitoSOX Red were purchased from Medchemexpress (Shanghai, China). .. Chlorin (Ce6), Mitochondria Isolation Kit, Cell Counting Kit-8, MitoTracker Green, 4’,6-diamidino-2phenylindole (DAPI), and JC-1 Mitochondrial Membrane Potential Assay Kit was obtained from Solarbio LIFE SCIENCES (Beijing, China). .. CellROX was purchased from Thermo Fisher Scientific (Massachusetts, USA).

    Mitochondrial Membrane Potential Assay:

    Article Title: Mitochondrial ‘Attack-and-Trap’ strategy potentiates cGAS-STING signaling to stimulate durable antitumor immunity against glioblastoma
    Article Snippet: Materials: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), cholesterol, DSPE-TK-PEG2000, Mdivi-1, 1,3-Diphenylisobenzofuran (DPBF), 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine,4chlorobenzenesulfonate Salt (DiD), 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO), and MitoSOX Red were purchased from Medchemexpress (Shanghai, China). .. Chlorin (Ce6), Mitochondria Isolation Kit, Cell Counting Kit-8, MitoTracker Green, 4’,6-diamidino-2phenylindole (DAPI), and JC-1 Mitochondrial Membrane Potential Assay Kit was obtained from Solarbio LIFE SCIENCES (Beijing, China). .. CellROX was purchased from Thermo Fisher Scientific (Massachusetts, USA).



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