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monarch hmw dna extraction kit  (New England Biolabs)


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    Structured Review

    New England Biolabs monarch hmw dna extraction kit
    Monarch Hmw Dna Extraction Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/extraction+kit/Monarch+HMW+DNA+Extraction+Kit+for+Cell/pm39862869-40-25-24
    Average 99 stars, based on 246 article reviews
    monarch hmw dna extraction kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6). .. BJ and IMR-90 cells were used as input for gDNA isolation using the NEB Monarch HMW DNA extraction kit for cells and blood (New England Biolabs, Cat. No. T3050L) following manufacturer’s instructions. .. HMW DNA was quantified by Nanodrop and QubitTM 1xdsDNA High Sensitivity kit (Thermo Fisher, Cat. No. Q33231 ).

    Article Title: Closely related, yet phenotypically different - Genome assemblies of two sister species of widow spiders: Latrodectus hasselti and L. katipo , Theridiidae
    Article Snippet: .. Genomic DNA for females of both species (two L. katipo and one L. hasselti ) used for long read as well as short read sequencing for L. hasselti was isolated using Monarch HMW DNA Extraction Kit for Tissue v2.1_4/21 (New England Biolabs). ..

    DNA Extraction:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6). .. BJ and IMR-90 cells were used as input for gDNA isolation using the NEB Monarch HMW DNA extraction kit for cells and blood (New England Biolabs, Cat. No. T3050L) following manufacturer’s instructions. .. HMW DNA was quantified by Nanodrop and QubitTM 1xdsDNA High Sensitivity kit (Thermo Fisher, Cat. No. Q33231 ).

    Article Title: Cultural transmission and genomic co-divergence in the willow tit across the Palearctic
    Article Snippet: .. High-molecular-weight DNA for PacBio HiFi sequencing was extracted with the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs). .. Illumina short-read libraries were prepared from the same individual.

    Article Title: The nuclear and mitochondrial genomes of the lined rockskipper, Istiblennius lineatus (Teleostei: Blenniidae).
    Article Snippet: .. 13 14 DNA was extracted from muscle tissue using the New England Biolabs High Molecular 15 Weight DNA extraction kit (Cat. #T3050L) following the manufacturer’s instructions. .. 16 Genomic DNA was sequenced on a Pacific Biosciences’ Revio 25M SMRT cell (HiFi 17 protocol) by the Uppsala Genome Centre, NGI-SciLifeLab (Uppsala, Sweden).

    Article Title: Population differences of chromosome 22q11.2 duplication structure predispose differentially to microdeletion and inversion.
    Article Snippet: .. Briefly, high-molecularweight (HMW) DNA was extracted from frozen blood or cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). .. At all steps, quantification was performed with Qubit dsDNA HS (Thermo Fisher, Q32854) measured on DS-11 FX (Denovix) and size distribution checked using FEMTO Pulse (Agilent, M5330AA & FP-1002-0275.)

    Article Title: MethylBench: A comprehensive benchmark of DNA methylation profiling methods across diverse sequencing platforms
    Article Snippet: .. DNA was extracted using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs). .. DNA from EDTA blood of 5 probands was extracted using the Flexigene kit (Qiagen) according to manufacturer’s instructions.

    Article Title: Closely related, yet phenotypically different - Genome assemblies of two sister species of widow spiders: Latrodectus hasselti and L. katipo , Theridiidae
    Article Snippet: .. Genomic DNA for females of both species (two L. katipo and one L. hasselti ) used for long read as well as short read sequencing for L. hasselti was isolated using Monarch HMW DNA Extraction Kit for Tissue v2.1_4/21 (New England Biolabs). ..

    Article Title: Structural Genomic Variation and Its Potential Role in Deer Speciation
    Article Snippet: .. DNA was extracted using the NEB Monarch HMW DNA Extraction Kit for Cells & Blood (#T3050) UHMW protocol, with some modifications to the cell lysis step. .. Specifically, cells were digested at 56°C for 1 h at 300 rpm with 100 μL Nuclei Prep Buffer, 100 μL Nuclei Lysis Buffer, 10 μL Proteinase K (NEB #P8107, 20 mg/mL), and 10 μL 1 M DTT (GoldBio #DTT in dH2O, final concentration ~50 mM).

    Sequencing:

    Article Title: Cultural transmission and genomic co-divergence in the willow tit across the Palearctic
    Article Snippet: .. High-molecular-weight DNA for PacBio HiFi sequencing was extracted with the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs). .. Illumina short-read libraries were prepared from the same individual.

    Article Title: Closely related, yet phenotypically different - Genome assemblies of two sister species of widow spiders: Latrodectus hasselti and L. katipo , Theridiidae
    Article Snippet: .. Genomic DNA for females of both species (two L. katipo and one L. hasselti ) used for long read as well as short read sequencing for L. hasselti was isolated using Monarch HMW DNA Extraction Kit for Tissue v2.1_4/21 (New England Biolabs). ..

    Cell Culture:

    Article Title: Population differences of chromosome 22q11.2 duplication structure predispose differentially to microdeletion and inversion.
    Article Snippet: .. Briefly, high-molecularweight (HMW) DNA was extracted from frozen blood or cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). .. At all steps, quantification was performed with Qubit dsDNA HS (Thermo Fisher, Q32854) measured on DS-11 FX (Denovix) and size distribution checked using FEMTO Pulse (Agilent, M5330AA & FP-1002-0275.)

    Lysis:

    Article Title: Structural Genomic Variation and Its Potential Role in Deer Speciation
    Article Snippet: .. DNA was extracted using the NEB Monarch HMW DNA Extraction Kit for Cells & Blood (#T3050) UHMW protocol, with some modifications to the cell lysis step. .. Specifically, cells were digested at 56°C for 1 h at 300 rpm with 100 μL Nuclei Prep Buffer, 100 μL Nuclei Lysis Buffer, 10 μL Proteinase K (NEB #P8107, 20 mg/mL), and 10 μL 1 M DTT (GoldBio #DTT in dH2O, final concentration ~50 mM).



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