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cell culture supernatants  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science cell culture supernatants
    Cell Culture Supernatants, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/extraction+kit/Cell+Supernatant+Exosome+Extraction+Kit/pm33554442-63-10-16
    Average 94 stars, based on 29 article reviews
    cell culture supernatants - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: PRRSV NSP5 orchestrates dual immune disruption by targeting NLRP3 and STING.
    Article Snippet: The reaction was terminated by adding the stop solution, and absorbance was determined using a microplate reader (Bio-Rad). .. For measuring IL-1β release, cell culture supernatants were collected and analyzed using the Porcine IL-1β ELISA Kit (Solarbio, #SEKP-0001). ..

    Article Title: Hexokinase 2-mediated histone H3K18la promotes PAI-1-dependent thrombosis in acute myeloid leukemia via tumor-endothelial crosstalk
    Article Snippet: The concentration of lactate in the patients’ plasma was measured using the Lactate Assay Kit (DiaSys, Germany) with a biochemistry analyzer (Mindray, China) according to the manufacturer’s instructions. .. For lactate in mouse plasma, cytoplasm or cell culture supernatants were detected using the L-Lactate Assay Kit (Solarbio, China) according to the manufacturer’s instruction. ..

    Article Title: An adenovirus-vectored recombinant vaccine confers passive immunity protection from swine acute diarrhea syndrome coronavirus challenge in neonatal mice
    Article Snippet: .. After 60 h of incubation, the concentrations of cytokine (IFN-γ and IL-4) in the cell culture supernatants were measured using IFN-γ ELISA kits (SEKP-0010, Solarbio) and IL-4 kits (SEKP-0003, Solarbio), in accordance with the manufacturers’ protocols. .. Data were analyzed using GraphPad Prism 8 with appropriate statistical tests (unpaired t -test, one-way or two-way ANOVA).

    Article Title: Hexokinase 2-mediated histone H3K18la promotes PAI-1-dependent thrombosis in acute myeloid leukemia via tumor-endothelial crosstalk.
    Article Snippet: The concentration of lactate in the patients’ plasma was measured using the Lactate Assay Kit (DiaSys, Germany) with a biochemistry analyzer (Mindray, China) according to the manufacturer’s instructions. .. For lactate in mouse plasma, cytoplasm or cell culture supernatants were detected using the L-Lactate Assay Kit (Solarbio, China) according to the manufacturer’s instruction. ..

    Article Title: PRRSV NSP5 orchestrates dual immune disruption by targeting NLRP3 and STING
    Article Snippet: The reaction was terminated by adding the stop solution, and absorbance was determined using a microplate reader (Bio-Rad). .. For measuring IL-1β release, cell culture supernatants were collected and analyzed using the Porcine IL-1β ELISA Kit (Solarbio, #SEKP-0001). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: PRRSV NSP5 orchestrates dual immune disruption by targeting NLRP3 and STING.
    Article Snippet: The reaction was terminated by adding the stop solution, and absorbance was determined using a microplate reader (Bio-Rad). .. For measuring IL-1β release, cell culture supernatants were collected and analyzed using the Porcine IL-1β ELISA Kit (Solarbio, #SEKP-0001). ..

    Article Title: MCT4 alleviates lipid accumulation, inflammation and PANoptosis in non-alcoholic fatty liver disease by inhibiting JAK-STAT signaling transduction
    Article Snippet: Finally, the protein bands were developed using the enhanced chemiluminescence (ECL) method (Pierce, Rockford, IL, USA), and the expression levels of the proteins were quantified using the ImageJ software (National Institutes of Health, USA). .. The concentrations of key cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6) and IL-8, in cell supernatants were determined by ELISA using kits from Solarbio (China), according to the manufacturer’s protocol. .. The levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were detected using relevant kits (Sigma).

    Article Title: An adenovirus-vectored recombinant vaccine confers passive immunity protection from swine acute diarrhea syndrome coronavirus challenge in neonatal mice
    Article Snippet: .. After 60 h of incubation, the concentrations of cytokine (IFN-γ and IL-4) in the cell culture supernatants were measured using IFN-γ ELISA kits (SEKP-0010, Solarbio) and IL-4 kits (SEKP-0003, Solarbio), in accordance with the manufacturers’ protocols. .. Data were analyzed using GraphPad Prism 8 with appropriate statistical tests (unpaired t -test, one-way or two-way ANOVA).

    Article Title: PRRSV NSP5 orchestrates dual immune disruption by targeting NLRP3 and STING
    Article Snippet: The reaction was terminated by adding the stop solution, and absorbance was determined using a microplate reader (Bio-Rad). .. For measuring IL-1β release, cell culture supernatants were collected and analyzed using the Porcine IL-1β ELISA Kit (Solarbio, #SEKP-0001). ..

    Clinical Proteomics:

    Article Title: Hexokinase 2-mediated histone H3K18la promotes PAI-1-dependent thrombosis in acute myeloid leukemia via tumor-endothelial crosstalk
    Article Snippet: The concentration of lactate in the patients’ plasma was measured using the Lactate Assay Kit (DiaSys, Germany) with a biochemistry analyzer (Mindray, China) according to the manufacturer’s instructions. .. For lactate in mouse plasma, cytoplasm or cell culture supernatants were detected using the L-Lactate Assay Kit (Solarbio, China) according to the manufacturer’s instruction. ..

    Article Title: Hexokinase 2-mediated histone H3K18la promotes PAI-1-dependent thrombosis in acute myeloid leukemia via tumor-endothelial crosstalk.
    Article Snippet: The concentration of lactate in the patients’ plasma was measured using the Lactate Assay Kit (DiaSys, Germany) with a biochemistry analyzer (Mindray, China) according to the manufacturer’s instructions. .. For lactate in mouse plasma, cytoplasm or cell culture supernatants were detected using the L-Lactate Assay Kit (Solarbio, China) according to the manufacturer’s instruction. ..

    other:

    Article Title: PKM2 preconditioning protects endothelial cells from pyroptosis and BBB disruption via NRF2/TRX/TXNIP signaling in neonatal hypoxic-ischemic brain injury
    Article Snippet: Cell supernatants were collected, and a commercial detection kit (Solarbio Biotechnology, China) was used to measure lactate levels.

    Bicinchoninic Acid Protein Assay:

    Article Title: Activation of IL-1 and IL-18 induced by Mycobacterium neoaurum and bacillus calmette-guérin are dependent on the NLRP3 and AIM2 inflammasome.
    Article Snippet: (Field and Cowie 2006; Koh et al. 2002).. Mycobacterium neoaurum (M. neoaurum) is a fast-growing nontuberculous mycobacteria (Moseley and Thind 2020).. M. neoaurum, as a zoonotic pathogen, can cause disease in many animals.

    Incubation:

    Article Title: An adenovirus-vectored recombinant vaccine confers passive immunity protection from swine acute diarrhea syndrome coronavirus challenge in neonatal mice
    Article Snippet: .. After 60 h of incubation, the concentrations of cytokine (IFN-γ and IL-4) in the cell culture supernatants were measured using IFN-γ ELISA kits (SEKP-0010, Solarbio) and IL-4 kits (SEKP-0003, Solarbio), in accordance with the manufacturers’ protocols. .. Data were analyzed using GraphPad Prism 8 with appropriate statistical tests (unpaired t -test, one-way or two-way ANOVA).



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