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qiaquick gel extraction kit  (Qiagen)


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    Structured Review

    Qiagen qiaquick gel extraction kit
    Qiaquick Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 7550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/extraction+kit/QIAquick+Gel+Extraction+Kit/pm42881182-489-11-15
    Average 96 stars, based on 7550 article reviews
    qiaquick gel extraction kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Immune responses and seasonal red sea bream iridovirus dynamics following formalin-inactivated vaccination in farmed rock bream (Oplegnathus fasciatus).
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Pervasive weak incompatibilities shape phenotypic variation and genome evolution in yeast hybrids.
    Article Snippet: .. Libraries were quantified, pooled at equal concentration and size-selected for fragments between 500 and 800bp by 2% agarose-gel electrophoresis and purification using the QIAquick Gel Extraction Kit (Qiagen). .. Fragment-size distributions were assessed using a BioAnalyzer 2100 with a High Sensitivity DNA chip (Agilent)48.

    Article Title: Reconstituting human and SV40 replisomes with Okazaki fragment maturation reveals synchronized leading- and lagging-strand synthesis.
    Article Snippet: ZN38 plasmid was digested with BsaI (20,000 units/ml, New England Biolabs) in a reaction containing 1 x CutSmart Buffer (New England Biolabs) for 2 h at 37 °C. .. The digested plasmid was purified from an agarose gel using the QIAquick Gel Extraction Kit (QIAGEN). .. The BsaIlinearized DNA was ligated to a 5-fold molar excess pre-annealed fork and incubated in a reaction containing T4 DNA ligase (60,000 units, New England Biolabs), 10x T4 ligase buffer at 16 °C for 17 h. The excess unligated fork was removed by gel purification.

    Article Title: High Genetic Variation in the COI Gene Complicates Molecular Identification of Eimeria Species in Goats from South Korea.
    Article Snippet: .. The amplified PCR products were confirmed via 1% agarose gel electrophoresis in Tris‐acetate‐EDTA (TAE) buffer and purified using a QIAquick Gel extraction kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions. ..

    Electrophoresis:

    Article Title: Immune responses and seasonal red sea bream iridovirus dynamics following formalin-inactivated vaccination in farmed rock bream (Oplegnathus fasciatus).
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Pervasive weak incompatibilities shape phenotypic variation and genome evolution in yeast hybrids.
    Article Snippet: .. Libraries were quantified, pooled at equal concentration and size-selected for fragments between 500 and 800bp by 2% agarose-gel electrophoresis and purification using the QIAquick Gel Extraction Kit (Qiagen). .. Fragment-size distributions were assessed using a BioAnalyzer 2100 with a High Sensitivity DNA chip (Agilent)48.

    Purification:

    Article Title: Immune responses and seasonal red sea bream iridovirus dynamics following formalin-inactivated vaccination in farmed rock bream (Oplegnathus fasciatus).
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Pervasive weak incompatibilities shape phenotypic variation and genome evolution in yeast hybrids.
    Article Snippet: .. Libraries were quantified, pooled at equal concentration and size-selected for fragments between 500 and 800bp by 2% agarose-gel electrophoresis and purification using the QIAquick Gel Extraction Kit (Qiagen). .. Fragment-size distributions were assessed using a BioAnalyzer 2100 with a High Sensitivity DNA chip (Agilent)48.

    Article Title: Reconstituting human and SV40 replisomes with Okazaki fragment maturation reveals synchronized leading- and lagging-strand synthesis.
    Article Snippet: ZN38 plasmid was digested with BsaI (20,000 units/ml, New England Biolabs) in a reaction containing 1 x CutSmart Buffer (New England Biolabs) for 2 h at 37 °C. .. The digested plasmid was purified from an agarose gel using the QIAquick Gel Extraction Kit (QIAGEN). .. The BsaIlinearized DNA was ligated to a 5-fold molar excess pre-annealed fork and incubated in a reaction containing T4 DNA ligase (60,000 units, New England Biolabs), 10x T4 ligase buffer at 16 °C for 17 h. The excess unligated fork was removed by gel purification.

    Article Title: Integrating gut microbiome and serum metabolomes to investigate the hepatoprotective effects of Lactiplantibacillus Plantarum PA01 against deoxynivalenol-induced liver injury in broilers.
    Article Snippet: .. PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN, NRW, Germany). .. Sequencing libraries were prepared and sequenced on an Illumina NovaSeq 6000 platform (PE300, Illumina, San Diego).

    Article Title: Rational attenuation of elapid long-chain neurotoxin by targeted residue substitution and disulfide architecture simplification: implications for receptor interaction and immune recognition.
    Article Snippet: Elapid long-chain neurotoxins (LNTxs) exert their toxicity through high-affinity binding to nicotinic acetylcholine receptors (nAChRs).. Specific residues mediate this process, including basic (Lys23, Arg33, Arg36, Lys49), structural (Cys26, Cys30), and aromatic (Phe29) amino acids.. To investigate the functional and immunogenic implications of modifying these residues, we designed two recombinant variants of a consensus LNTx.

    Article Title: Water-Sediment Partitioning of a Neutrophilic Iron-Cycling Microbiome in the High-Altitude Dzhily-Su Mineral Springs (Mt. Elbrus).
    Article Snippet: .. The amplicon libraries were purified using a QIAquick Gel Extraction Kit (Qiagen, Germany), and checked using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). .. The final libraries were pooled in equimolar amounts and sequenced on an Illumina MiSeq platform in paired-end mode (2 × 156 bp).

    Article Title: Borrelia miyamotoi exceeds Borrelia burgdorferi sensu lato in human exposure despite the opposite in ticks: an integrated serological, entomological and molecular study in southeastern Kazakhstan.
    Article Snippet: Each locus was amplified in two consecutive rounds of conventional PCR with a high-fidelity polymerase (Platinum Taq, Invitrogen, USA), following the supplier's protocol. .. Products were separated in 1.5% agarose gels cast in 1× Tris-acetate buffer containing 0.5 μg/mL ethidium bromide, run on an EasyCast D2 system (Fisher Scientific, USA), then excised and purified with the QIAquick Gel Extraction Kit (Qiagen, USA). .. Purified amplicons were sequenced by the Sanger method (BigDye Terminator v3.1, Applied Biosystems, USA) on a 24-capillary ABI 3500xl instrument, and base calls were reviewed in Sequencing Analysis v5.2.

    Article Title: High Genetic Variation in the COI Gene Complicates Molecular Identification of Eimeria Species in Goats from South Korea.
    Article Snippet: .. The amplified PCR products were confirmed via 1% agarose gel electrophoresis in Tris‐acetate‐EDTA (TAE) buffer and purified using a QIAquick Gel extraction kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions. ..

    Gel Extraction:

    Article Title: Immune responses and seasonal red sea bream iridovirus dynamics following formalin-inactivated vaccination in farmed rock bream (Oplegnathus fasciatus).
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Pervasive weak incompatibilities shape phenotypic variation and genome evolution in yeast hybrids.
    Article Snippet: .. Libraries were quantified, pooled at equal concentration and size-selected for fragments between 500 and 800bp by 2% agarose-gel electrophoresis and purification using the QIAquick Gel Extraction Kit (Qiagen). .. Fragment-size distributions were assessed using a BioAnalyzer 2100 with a High Sensitivity DNA chip (Agilent)48.

    Article Title: Reconstituting human and SV40 replisomes with Okazaki fragment maturation reveals synchronized leading- and lagging-strand synthesis.
    Article Snippet: ZN38 plasmid was digested with BsaI (20,000 units/ml, New England Biolabs) in a reaction containing 1 x CutSmart Buffer (New England Biolabs) for 2 h at 37 °C. .. The digested plasmid was purified from an agarose gel using the QIAquick Gel Extraction Kit (QIAGEN). .. The BsaIlinearized DNA was ligated to a 5-fold molar excess pre-annealed fork and incubated in a reaction containing T4 DNA ligase (60,000 units, New England Biolabs), 10x T4 ligase buffer at 16 °C for 17 h. The excess unligated fork was removed by gel purification.

    Article Title: Integrating gut microbiome and serum metabolomes to investigate the hepatoprotective effects of Lactiplantibacillus Plantarum PA01 against deoxynivalenol-induced liver injury in broilers.
    Article Snippet: .. PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN, NRW, Germany). .. Sequencing libraries were prepared and sequenced on an Illumina NovaSeq 6000 platform (PE300, Illumina, San Diego).

    Article Title: Rational attenuation of elapid long-chain neurotoxin by targeted residue substitution and disulfide architecture simplification: implications for receptor interaction and immune recognition.
    Article Snippet: Elapid long-chain neurotoxins (LNTxs) exert their toxicity through high-affinity binding to nicotinic acetylcholine receptors (nAChRs).. Specific residues mediate this process, including basic (Lys23, Arg33, Arg36, Lys49), structural (Cys26, Cys30), and aromatic (Phe29) amino acids.. To investigate the functional and immunogenic implications of modifying these residues, we designed two recombinant variants of a consensus LNTx.

    Article Title: Water-Sediment Partitioning of a Neutrophilic Iron-Cycling Microbiome in the High-Altitude Dzhily-Su Mineral Springs (Mt. Elbrus).
    Article Snippet: .. The amplicon libraries were purified using a QIAquick Gel Extraction Kit (Qiagen, Germany), and checked using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). .. The final libraries were pooled in equimolar amounts and sequenced on an Illumina MiSeq platform in paired-end mode (2 × 156 bp).

    Article Title: Borrelia miyamotoi exceeds Borrelia burgdorferi sensu lato in human exposure despite the opposite in ticks: an integrated serological, entomological and molecular study in southeastern Kazakhstan.
    Article Snippet: Each locus was amplified in two consecutive rounds of conventional PCR with a high-fidelity polymerase (Platinum Taq, Invitrogen, USA), following the supplier's protocol. .. Products were separated in 1.5% agarose gels cast in 1× Tris-acetate buffer containing 0.5 μg/mL ethidium bromide, run on an EasyCast D2 system (Fisher Scientific, USA), then excised and purified with the QIAquick Gel Extraction Kit (Qiagen, USA). .. Purified amplicons were sequenced by the Sanger method (BigDye Terminator v3.1, Applied Biosystems, USA) on a 24-capillary ABI 3500xl instrument, and base calls were reviewed in Sequencing Analysis v5.2.

    Article Title: High Genetic Variation in the COI Gene Complicates Molecular Identification of Eimeria Species in Goats from South Korea.
    Article Snippet: .. The amplified PCR products were confirmed via 1% agarose gel electrophoresis in Tris‐acetate‐EDTA (TAE) buffer and purified using a QIAquick Gel extraction kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions. ..

    Concentration Assay:

    Article Title: Pervasive weak incompatibilities shape phenotypic variation and genome evolution in yeast hybrids.
    Article Snippet: .. Libraries were quantified, pooled at equal concentration and size-selected for fragments between 500 and 800bp by 2% agarose-gel electrophoresis and purification using the QIAquick Gel Extraction Kit (Qiagen). .. Fragment-size distributions were assessed using a BioAnalyzer 2100 with a High Sensitivity DNA chip (Agilent)48.

    Plasmid Preparation:

    Article Title: Reconstituting human and SV40 replisomes with Okazaki fragment maturation reveals synchronized leading- and lagging-strand synthesis.
    Article Snippet: ZN38 plasmid was digested with BsaI (20,000 units/ml, New England Biolabs) in a reaction containing 1 x CutSmart Buffer (New England Biolabs) for 2 h at 37 °C. .. The digested plasmid was purified from an agarose gel using the QIAquick Gel Extraction Kit (QIAGEN). .. The BsaIlinearized DNA was ligated to a 5-fold molar excess pre-annealed fork and incubated in a reaction containing T4 DNA ligase (60,000 units, New England Biolabs), 10x T4 ligase buffer at 16 °C for 17 h. The excess unligated fork was removed by gel purification.

    Polymerase Chain Reaction:

    Article Title: Integrating gut microbiome and serum metabolomes to investigate the hepatoprotective effects of Lactiplantibacillus Plantarum PA01 against deoxynivalenol-induced liver injury in broilers.
    Article Snippet: .. PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN, NRW, Germany). .. Sequencing libraries were prepared and sequenced on an Illumina NovaSeq 6000 platform (PE300, Illumina, San Diego).

    Article Title: High Genetic Variation in the COI Gene Complicates Molecular Identification of Eimeria Species in Goats from South Korea.
    Article Snippet: .. The amplified PCR products were confirmed via 1% agarose gel electrophoresis in Tris‐acetate‐EDTA (TAE) buffer and purified using a QIAquick Gel extraction kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions. ..

    Amplification:

    Article Title: Rational attenuation of elapid long-chain neurotoxin by targeted residue substitution and disulfide architecture simplification: implications for receptor interaction and immune recognition.
    Article Snippet: Elapid long-chain neurotoxins (LNTxs) exert their toxicity through high-affinity binding to nicotinic acetylcholine receptors (nAChRs).. Specific residues mediate this process, including basic (Lys23, Arg33, Arg36, Lys49), structural (Cys26, Cys30), and aromatic (Phe29) amino acids.. To investigate the functional and immunogenic implications of modifying these residues, we designed two recombinant variants of a consensus LNTx.

    Article Title: Water-Sediment Partitioning of a Neutrophilic Iron-Cycling Microbiome in the High-Altitude Dzhily-Su Mineral Springs (Mt. Elbrus).
    Article Snippet: .. The amplicon libraries were purified using a QIAquick Gel Extraction Kit (Qiagen, Germany), and checked using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). .. The final libraries were pooled in equimolar amounts and sequenced on an Illumina MiSeq platform in paired-end mode (2 × 156 bp).

    Article Title: High Genetic Variation in the COI Gene Complicates Molecular Identification of Eimeria Species in Goats from South Korea.
    Article Snippet: .. The amplified PCR products were confirmed via 1% agarose gel electrophoresis in Tris‐acetate‐EDTA (TAE) buffer and purified using a QIAquick Gel extraction kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions. ..



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