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nuclei lysis buffer  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec nuclei lysis buffer
    Nuclei Lysis Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/extraction+buffer/Nuclei+Extraction+Buffer/pmc13378589-245-8-14
    Average 96 stars, based on 115 article reviews
    nuclei lysis buffer - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Extraction:

    Article Title: Protocol optimization to process mouse colon samples for single-nuclei RNA sequencing using FLEX library preparation
    Article Snippet: 10% BSA , Sigma Aldrich , 126615. .. Nuclei extraction buffer , Miltenyi , 130-128-024. .. Acridine Orange , Revvity , CS1-0108.

    Article Title: SORLA up-regulation suppresses pathological effects in aged tauopathy mouse brain
    Article Snippet: .. Three frozen 9 MO hippocampus tissue from WT, PS19, and SORLA TG/PS19 groups were pooled and lysed in Miltenyi nuclei extraction buffer containing RNase inhibitor (0.2 U/μl) using a gentleMACS Octo Dissociator (Program 4C_nuclei_1) according to the manufacturer’s protocol. .. Miltenyi Debris removal solution was applied to the nuclei suspension, and NeuN-AF488 and Olig2-AF647 were added to the samples and incubated on ice for 30 min.

    Article Title: Tricuspid valve regurgitation accelerates heart failure via a cardio-intestinal innate immune circuit
    Article Snippet: .. Snap-frozen cardiac tissue samples were transferred into 2 ml of ice-cold Nuclei Extraction Buffer (Miltenyi Biotec, #130-128-024) buffer supplemented with 0.2 U/μl RNAse inhibitor (Invitrogen, AM2696) in gentleMACS C Tubes (Miltenyi Biotec, #130-093-237). .. Tubes were placed on a gentleMACS Octo Dissociator (Miltenyi Biotec, #130-095-937), and the 4C_nuclei_1 program was run.

    Article Title: Patient-derived tau-seeded human neuronal chimeras recapitulate mature Alzheimer’s tau pathology and uncover human-specific neuronal vulnerability
    Article Snippet: Mice were euthanized and perfused with ice-cold DPBS, and the brains were quickly extracted and rinsed in the same buffer supplied with RiboGrip RNase inhibitor (Solis BioDyne, 0.8 U/μL). .. Brains were then dissociated and chopped into fine pieces before being transferred into Nuclei Extraction Buffer (Miltenyi Biotec, 130-128-024). .. Tissues were subsequently processed using gentleMACS dissociator (Miltenyi Biotec) following instructions.

    Article Title: Single-cell and spatial omics reveals region-specific plasticity and therapeutic vulnerabilities in metastatic high-risk neuroblastoma
    Article Snippet: .. Snap-frozen human NBL specimens were lysed in ice-cold nuclei lysis buffer (Nuclei Extraction Buffer (Miltenyi Biotec) containing dithiothreitol (DTT) (Sigma-Aldrich) and (RNase) inhibitor (Sigma-Aldrich) for 5 min on a gentleMACS Octo Dissociator at 4°C with agitation. ..

    Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing.
    Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, mouse MILTENYI BIOTEC Cat130-105-807 Anti-Nucleus MicroBeads MILTENYI BIOTEC Cat#130-132-997 BD RhapsodyTM cDNA Kit BD Biosciences Cat#633773 BD RhapsodyTM Enhanced Cartridge Reagent Kit BD Biosciences Cat#667052 BD RhapsodyTM 8-Lane Cartridge BD Biosciences Cat#666262 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat#633801 QubitTM dsDNA HS Assay Kit Thermo Fisher Cat#Q32851 Agilent High Sensitivity DNA Kit Agilent Cat#5067-4626 MACS BSA Stock Solution MILTENYI BIOTEC Cat#130-091-376 BD® OMICS-One Dual Index Kit BD Biosciences Cat#571899 Agencourt® AMPure® XP magnetic beads Beckman Coulter Cat#A63880 Other LS Columns MILTENYI BIOTEC Cat#130-042-401 MACS SmartStrainers (30 μm) MILTENYI BIOTEC Cat#130-098-458 MACS SmartStrainers (70 μm) MILTENYI BIOTEC Cat#130-098-462 MACS SmartStrainers (100 μm) MILTENYI BIOTEC Cat#130-098-463 GentleMACS C Tubes MILTENYI BIOTEC Cat#130-093-237 Eppendorf® LoBind® Tubes BD Biosciences Cat#570751 Hemocytometer NANOENTEK Cat#DHC-N01 Hemocytometer adapter BD Biosciences Cat#633703 Centrifuge and rotor with adapters for 5-mL Falcon® tubes and 15-mL tubes. ..

    Lysis:

    Article Title: Single-cell and spatial omics reveals region-specific plasticity and therapeutic vulnerabilities in metastatic high-risk neuroblastoma
    Article Snippet: .. Snap-frozen human NBL specimens were lysed in ice-cold nuclei lysis buffer (Nuclei Extraction Buffer (Miltenyi Biotec) containing dithiothreitol (DTT) (Sigma-Aldrich) and (RNase) inhibitor (Sigma-Aldrich) for 5 min on a gentleMACS Octo Dissociator at 4°C with agitation. ..

    RNA Sequencing:

    Article Title: Transcriptional plasticity of ventral tegmental area neurons induced after cessation of chronic cocaine exposure is required for incubation of cocaine seeking
    Article Snippet: .. For single nucleus RNA sequencing (snRNA-seq) experiments, the nuclei fraction was pelleted and resuspended in MACS nuclei separation buffer (Miltenyi Biotec, 130-128-024). .. For single nucleus RNA sequencing (snRNA-seq) experiments, the nuclei fraction was pelleted and resuspended in MACS nuclei separation buffer (Miltenyi Biotec, 130-128-024).

    Magnetic Cell Separation:

    Article Title: Transcriptional plasticity of ventral tegmental area neurons induced after cessation of chronic cocaine exposure is required for incubation of cocaine seeking
    Article Snippet: .. For single nucleus RNA sequencing (snRNA-seq) experiments, the nuclei fraction was pelleted and resuspended in MACS nuclei separation buffer (Miltenyi Biotec, 130-128-024). .. For single nucleus RNA sequencing (snRNA-seq) experiments, the nuclei fraction was pelleted and resuspended in MACS nuclei separation buffer (Miltenyi Biotec, 130-128-024).

    Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing.
    Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, mouse MILTENYI BIOTEC Cat130-105-807 Anti-Nucleus MicroBeads MILTENYI BIOTEC Cat#130-132-997 BD RhapsodyTM cDNA Kit BD Biosciences Cat#633773 BD RhapsodyTM Enhanced Cartridge Reagent Kit BD Biosciences Cat#667052 BD RhapsodyTM 8-Lane Cartridge BD Biosciences Cat#666262 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat#633801 QubitTM dsDNA HS Assay Kit Thermo Fisher Cat#Q32851 Agilent High Sensitivity DNA Kit Agilent Cat#5067-4626 MACS BSA Stock Solution MILTENYI BIOTEC Cat#130-091-376 BD® OMICS-One Dual Index Kit BD Biosciences Cat#571899 Agencourt® AMPure® XP magnetic beads Beckman Coulter Cat#A63880 Other LS Columns MILTENYI BIOTEC Cat#130-042-401 MACS SmartStrainers (30 μm) MILTENYI BIOTEC Cat#130-098-458 MACS SmartStrainers (70 μm) MILTENYI BIOTEC Cat#130-098-462 MACS SmartStrainers (100 μm) MILTENYI BIOTEC Cat#130-098-463 GentleMACS C Tubes MILTENYI BIOTEC Cat#130-093-237 Eppendorf® LoBind® Tubes BD Biosciences Cat#570751 Hemocytometer NANOENTEK Cat#DHC-N01 Hemocytometer adapter BD Biosciences Cat#633703 Centrifuge and rotor with adapters for 5-mL Falcon® tubes and 15-mL tubes. ..

    Western Blot:

    Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing.
    Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, mouse MILTENYI BIOTEC Cat130-105-807 Anti-Nucleus MicroBeads MILTENYI BIOTEC Cat#130-132-997 BD RhapsodyTM cDNA Kit BD Biosciences Cat#633773 BD RhapsodyTM Enhanced Cartridge Reagent Kit BD Biosciences Cat#667052 BD RhapsodyTM 8-Lane Cartridge BD Biosciences Cat#666262 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat#633801 QubitTM dsDNA HS Assay Kit Thermo Fisher Cat#Q32851 Agilent High Sensitivity DNA Kit Agilent Cat#5067-4626 MACS BSA Stock Solution MILTENYI BIOTEC Cat#130-091-376 BD® OMICS-One Dual Index Kit BD Biosciences Cat#571899 Agencourt® AMPure® XP magnetic beads Beckman Coulter Cat#A63880 Other LS Columns MILTENYI BIOTEC Cat#130-042-401 MACS SmartStrainers (30 μm) MILTENYI BIOTEC Cat#130-098-458 MACS SmartStrainers (70 μm) MILTENYI BIOTEC Cat#130-098-462 MACS SmartStrainers (100 μm) MILTENYI BIOTEC Cat#130-098-463 GentleMACS C Tubes MILTENYI BIOTEC Cat#130-093-237 Eppendorf® LoBind® Tubes BD Biosciences Cat#570751 Hemocytometer NANOENTEK Cat#DHC-N01 Hemocytometer adapter BD Biosciences Cat#633703 Centrifuge and rotor with adapters for 5-mL Falcon® tubes and 15-mL tubes. ..

    Recombinant:

    Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing.
    Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, mouse MILTENYI BIOTEC Cat130-105-807 Anti-Nucleus MicroBeads MILTENYI BIOTEC Cat#130-132-997 BD RhapsodyTM cDNA Kit BD Biosciences Cat#633773 BD RhapsodyTM Enhanced Cartridge Reagent Kit BD Biosciences Cat#667052 BD RhapsodyTM 8-Lane Cartridge BD Biosciences Cat#666262 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat#633801 QubitTM dsDNA HS Assay Kit Thermo Fisher Cat#Q32851 Agilent High Sensitivity DNA Kit Agilent Cat#5067-4626 MACS BSA Stock Solution MILTENYI BIOTEC Cat#130-091-376 BD® OMICS-One Dual Index Kit BD Biosciences Cat#571899 Agencourt® AMPure® XP magnetic beads Beckman Coulter Cat#A63880 Other LS Columns MILTENYI BIOTEC Cat#130-042-401 MACS SmartStrainers (30 μm) MILTENYI BIOTEC Cat#130-098-458 MACS SmartStrainers (70 μm) MILTENYI BIOTEC Cat#130-098-462 MACS SmartStrainers (100 μm) MILTENYI BIOTEC Cat#130-098-463 GentleMACS C Tubes MILTENYI BIOTEC Cat#130-093-237 Eppendorf® LoBind® Tubes BD Biosciences Cat#570751 Hemocytometer NANOENTEK Cat#DHC-N01 Hemocytometer adapter BD Biosciences Cat#633703 Centrifuge and rotor with adapters for 5-mL Falcon® tubes and 15-mL tubes. ..

    Staining:

    Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing.
    Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, mouse MILTENYI BIOTEC Cat130-105-807 Anti-Nucleus MicroBeads MILTENYI BIOTEC Cat#130-132-997 BD RhapsodyTM cDNA Kit BD Biosciences Cat#633773 BD RhapsodyTM Enhanced Cartridge Reagent Kit BD Biosciences Cat#667052 BD RhapsodyTM 8-Lane Cartridge BD Biosciences Cat#666262 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat#633801 QubitTM dsDNA HS Assay Kit Thermo Fisher Cat#Q32851 Agilent High Sensitivity DNA Kit Agilent Cat#5067-4626 MACS BSA Stock Solution MILTENYI BIOTEC Cat#130-091-376 BD® OMICS-One Dual Index Kit BD Biosciences Cat#571899 Agencourt® AMPure® XP magnetic beads Beckman Coulter Cat#A63880 Other LS Columns MILTENYI BIOTEC Cat#130-042-401 MACS SmartStrainers (30 μm) MILTENYI BIOTEC Cat#130-098-458 MACS SmartStrainers (70 μm) MILTENYI BIOTEC Cat#130-098-462 MACS SmartStrainers (100 μm) MILTENYI BIOTEC Cat#130-098-463 GentleMACS C Tubes MILTENYI BIOTEC Cat#130-093-237 Eppendorf® LoBind® Tubes BD Biosciences Cat#570751 Hemocytometer NANOENTEK Cat#DHC-N01 Hemocytometer adapter BD Biosciences Cat#633703 Centrifuge and rotor with adapters for 5-mL Falcon® tubes and 15-mL tubes. ..

    Amplification:

    Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing.
    Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, mouse MILTENYI BIOTEC Cat130-105-807 Anti-Nucleus MicroBeads MILTENYI BIOTEC Cat#130-132-997 BD RhapsodyTM cDNA Kit BD Biosciences Cat#633773 BD RhapsodyTM Enhanced Cartridge Reagent Kit BD Biosciences Cat#667052 BD RhapsodyTM 8-Lane Cartridge BD Biosciences Cat#666262 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat#633801 QubitTM dsDNA HS Assay Kit Thermo Fisher Cat#Q32851 Agilent High Sensitivity DNA Kit Agilent Cat#5067-4626 MACS BSA Stock Solution MILTENYI BIOTEC Cat#130-091-376 BD® OMICS-One Dual Index Kit BD Biosciences Cat#571899 Agencourt® AMPure® XP magnetic beads Beckman Coulter Cat#A63880 Other LS Columns MILTENYI BIOTEC Cat#130-042-401 MACS SmartStrainers (30 μm) MILTENYI BIOTEC Cat#130-098-458 MACS SmartStrainers (70 μm) MILTENYI BIOTEC Cat#130-098-462 MACS SmartStrainers (100 μm) MILTENYI BIOTEC Cat#130-098-463 GentleMACS C Tubes MILTENYI BIOTEC Cat#130-093-237 Eppendorf® LoBind® Tubes BD Biosciences Cat#570751 Hemocytometer NANOENTEK Cat#DHC-N01 Hemocytometer adapter BD Biosciences Cat#633703 Centrifuge and rotor with adapters for 5-mL Falcon® tubes and 15-mL tubes. ..

    Magnetic Beads:

    Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing.
    Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, mouse MILTENYI BIOTEC Cat130-105-807 Anti-Nucleus MicroBeads MILTENYI BIOTEC Cat#130-132-997 BD RhapsodyTM cDNA Kit BD Biosciences Cat#633773 BD RhapsodyTM Enhanced Cartridge Reagent Kit BD Biosciences Cat#667052 BD RhapsodyTM 8-Lane Cartridge BD Biosciences Cat#666262 BD RhapsodyTM WTA Amplification Kit BD Biosciences Cat#633801 QubitTM dsDNA HS Assay Kit Thermo Fisher Cat#Q32851 Agilent High Sensitivity DNA Kit Agilent Cat#5067-4626 MACS BSA Stock Solution MILTENYI BIOTEC Cat#130-091-376 BD® OMICS-One Dual Index Kit BD Biosciences Cat#571899 Agencourt® AMPure® XP magnetic beads Beckman Coulter Cat#A63880 Other LS Columns MILTENYI BIOTEC Cat#130-042-401 MACS SmartStrainers (30 μm) MILTENYI BIOTEC Cat#130-098-458 MACS SmartStrainers (70 μm) MILTENYI BIOTEC Cat#130-098-462 MACS SmartStrainers (100 μm) MILTENYI BIOTEC Cat#130-098-463 GentleMACS C Tubes MILTENYI BIOTEC Cat#130-093-237 Eppendorf® LoBind® Tubes BD Biosciences Cat#570751 Hemocytometer NANOENTEK Cat#DHC-N01 Hemocytometer adapter BD Biosciences Cat#633703 Centrifuge and rotor with adapters for 5-mL Falcon® tubes and 15-mL tubes. ..



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