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Lucigen Corp quick extract qe buffer
Quick Extract Qe Buffer, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/extraction+buffer/buffer+quickextract/pm42259299-309-21-25
Average 86 stars, based on 1 article reviews
quick extract qe buffer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Codon-optimized base editors enable efficient base substitution in Atlantic salmon.
Article Snippet: .. DNA was extracted using QuickExtract buffer (Lucigen, Middleton, WI, USA) and then subjected to a PCR reaction to amplify o n- and off-target regions of each gene using specific primers (Table S2). ..

Article Title: Ribosome-associated quality control of aberrant protein production during amino acid limitation
Article Snippet: Two sgRNAs were designed in introns flanking the first coding exon that was not a multiple of 3 in length and cloned into the targeting plasmids pU6-(BbsI)_CBh-Cas9-T2A-BFP (#64323) and pSpCas9(BB)-2A-GFP (Addgene #48138). .. Knockout clones were verified by genomic DNA PCR using cell pellets lysed in QuickExtract buffer (Lucigen) according to the manufacturer’s instructions, using primers flanking the sgRNA binding sites; and/or by western blotting. ..

other:

Article Title: Modified immune cells having adenosine deaminase base editors for modifying a nucleobase in a target sequence
Article Snippet: The resulting PCR product was purified on a Zymo Research 25 μg DCC column and used as mRNA template in the subsequent in vitro transcription.

Article Title: Base editing enzymes
Article Snippet: To construct the MGA (Metagenomi adenine base editor) entry plasmid containing T7 promoter-His tag-TadA*(ABE8.17m)-SV40 NLS, three DNA fragments were amplified from pAL6.

Plasmid Preparation:

Article Title: Structure and engineering of the large serine recombinase Bxb1 for gene integration.
Article Snippet: Article Structure and engineering of the large serine recombinase Bxb1 for gene integration

Knock-Out:

Article Title: Ribosome-associated quality control of aberrant protein production during amino acid limitation
Article Snippet: Two sgRNAs were designed in introns flanking the first coding exon that was not a multiple of 3 in length and cloned into the targeting plasmids pU6-(BbsI)_CBh-Cas9-T2A-BFP (#64323) and pSpCas9(BB)-2A-GFP (Addgene #48138). .. Knockout clones were verified by genomic DNA PCR using cell pellets lysed in QuickExtract buffer (Lucigen) according to the manufacturer’s instructions, using primers flanking the sgRNA binding sites; and/or by western blotting. ..

Clone Assay:

Article Title: Ribosome-associated quality control of aberrant protein production during amino acid limitation
Article Snippet: Two sgRNAs were designed in introns flanking the first coding exon that was not a multiple of 3 in length and cloned into the targeting plasmids pU6-(BbsI)_CBh-Cas9-T2A-BFP (#64323) and pSpCas9(BB)-2A-GFP (Addgene #48138). .. Knockout clones were verified by genomic DNA PCR using cell pellets lysed in QuickExtract buffer (Lucigen) according to the manufacturer’s instructions, using primers flanking the sgRNA binding sites; and/or by western blotting. ..

Binding Assay:

Article Title: Ribosome-associated quality control of aberrant protein production during amino acid limitation
Article Snippet: Two sgRNAs were designed in introns flanking the first coding exon that was not a multiple of 3 in length and cloned into the targeting plasmids pU6-(BbsI)_CBh-Cas9-T2A-BFP (#64323) and pSpCas9(BB)-2A-GFP (Addgene #48138). .. Knockout clones were verified by genomic DNA PCR using cell pellets lysed in QuickExtract buffer (Lucigen) according to the manufacturer’s instructions, using primers flanking the sgRNA binding sites; and/or by western blotting. ..

Western Blot:

Article Title: Ribosome-associated quality control of aberrant protein production during amino acid limitation
Article Snippet: Two sgRNAs were designed in introns flanking the first coding exon that was not a multiple of 3 in length and cloned into the targeting plasmids pU6-(BbsI)_CBh-Cas9-T2A-BFP (#64323) and pSpCas9(BB)-2A-GFP (Addgene #48138). .. Knockout clones were verified by genomic DNA PCR using cell pellets lysed in QuickExtract buffer (Lucigen) according to the manufacturer’s instructions, using primers flanking the sgRNA binding sites; and/or by western blotting. ..

Electroporation:

Article Title: A commonly inherited human PCSK9 germline variant drives breast cancer metastasis via LRP1 receptor
Article Snippet: .. 24 hours after electroporation each 2-cell embryo was transferred to 6ul of QuickExtract buffer (Lucigen, QE09050), heated to 68°C for 6 minutes, and then 98°C for 2 minutes. ..



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