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gst bacterial expression vector pgex 6p 1  (Cytiva Europe)


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    Cytiva Europe gst bacterial expression vector pgex 6p 1
    Gst Bacterial Expression Vector Pgex 6p 1, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector/PGEX-6P-1+Vector/us12653805-381-12-17
    Average 95 stars, based on 834 article reviews
    gst bacterial expression vector pgex 6p 1 - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: Bisphenol BPAF and BPC are agonists for estrogen receptor ERα but antagonists for N-terminal domain-lacking ERα
    Article Snippet: Briefly, a cDNA fragment encoding ERα-LBD (residues 303‒595) was amplified by PCR and cloned into the pGEX-6p-1 vector (GE Healthcare BioSciences, Piscataway, NJ, USA) for GST fusion, and the resulting GST-ERα-LBD construct was expressed in E. coli BL21α.

    Article Title: Targeting LASP1, eIF4A1, eIF4B and CXC4 with modulators and combinations thereof for cancer therapy
    Article Snippet: The verity of the DNA construct was confirmed by sequencing (Eurofins Genomics, Louisville, Ky.).

    Clone Assay:

    Article Title: Two Plasmodium vivax hypnozoite-expressed RNA-binding proteins inhibit liver stage replication.
    Article Snippet: The liver stage counts and sizes were quantified using the ‘Hybrid Duo Count and Measure’ function in Keyence Microscopy (BZ-X710). .. For IESI-1, gene sequences that correspond to homologous regions containing the annotated RRM for orthologues from P. falciparum (aa 875-1232), P. vivax (aa 896-1203) and P. yoelii (aa 795-956) were cloned into the pGEX6P1 (Cytiva, cat. no. 28-9546-48) vector for expression with an N-terminal GST-tag. .. Sequences corresponding to aa 1-109 containing the three CCCH zinc finger domains of IESI-2 orthologues from P. falciparum, P. vivax and P. yoelii were also cloned downstream of a GST-tag in the pGEX6P1 (Cytiva, cat. no. 28-9546-48) vector for recombinant protein expression in bacteria, E. coli Rosetta 2 (DE3)pLysS (EMD Millipore, cat. no. 71401).

    Article Title: Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimp.
    Article Snippet: .. 639 640 Plasmid constructs, protein expression, and purification 641 The coding sequences for the functional domains of P. vannamei O-GlcNAc transferase 642 (PvOGT, GenBank: MH797025.1) and O-GlcNAcase (PvOGA, GenBank: 643 MH797026.1) were cloned into the pGEX-6P-1 vector (GE Healthcare, Boston, MA, 644 Jo urn al Pr e-p roo f 23 USA, 28-9546-48) at the BamHI site using the ClonExpress II One Step Cloning Kit 645 (Vazyme, Nanjing, China, C112). ..

    Article Title: The CCCH-type zinc-finger Pf MD3 promotes translation for malaria parasite transmission
    Article Snippet: .. For in vitro RNA-binding assays, the full length, short (aa 140-214), medium (aa 1-214) and long (aa 140-485) versions of Pf MD3 were cloned downstream of a GST-tag in the pGEX6P1 (Cytiva, 28-9546-48) vector for recombinant protein expression in bacteria, E. coli BL21(DE3)pLysS (Promega, L1195). ..

    Expressing:

    Article Title: Two Plasmodium vivax hypnozoite-expressed RNA-binding proteins inhibit liver stage replication.
    Article Snippet: The liver stage counts and sizes were quantified using the ‘Hybrid Duo Count and Measure’ function in Keyence Microscopy (BZ-X710). .. For IESI-1, gene sequences that correspond to homologous regions containing the annotated RRM for orthologues from P. falciparum (aa 875-1232), P. vivax (aa 896-1203) and P. yoelii (aa 795-956) were cloned into the pGEX6P1 (Cytiva, cat. no. 28-9546-48) vector for expression with an N-terminal GST-tag. .. Sequences corresponding to aa 1-109 containing the three CCCH zinc finger domains of IESI-2 orthologues from P. falciparum, P. vivax and P. yoelii were also cloned downstream of a GST-tag in the pGEX6P1 (Cytiva, cat. no. 28-9546-48) vector for recombinant protein expression in bacteria, E. coli Rosetta 2 (DE3)pLysS (EMD Millipore, cat. no. 71401).

    Article Title: Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimp.
    Article Snippet: .. 639 640 Plasmid constructs, protein expression, and purification 641 The coding sequences for the functional domains of P. vannamei O-GlcNAc transferase 642 (PvOGT, GenBank: MH797025.1) and O-GlcNAcase (PvOGA, GenBank: 643 MH797026.1) were cloned into the pGEX-6P-1 vector (GE Healthcare, Boston, MA, 644 Jo urn al Pr e-p roo f 23 USA, 28-9546-48) at the BamHI site using the ClonExpress II One Step Cloning Kit 645 (Vazyme, Nanjing, China, C112). ..

    Article Title: The CCCH-type zinc-finger Pf MD3 promotes translation for malaria parasite transmission
    Article Snippet: .. For in vitro RNA-binding assays, the full length, short (aa 140-214), medium (aa 1-214) and long (aa 140-485) versions of Pf MD3 were cloned downstream of a GST-tag in the pGEX6P1 (Cytiva, 28-9546-48) vector for recombinant protein expression in bacteria, E. coli BL21(DE3)pLysS (Promega, L1195). ..

    Plasmid Preparation:

    Article Title: FKF1 nuclear condensates control anti-florigen turnover and flowering onset in response to nitrogen availability in monocots.
    Article Snippet: Article FKF1 nuclear condensates control anti-florigen turnover and flowering onset in response to nitrogen availability in monocots

    Article Title: Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimp.
    Article Snippet: .. 639 640 Plasmid constructs, protein expression, and purification 641 The coding sequences for the functional domains of P. vannamei O-GlcNAc transferase 642 (PvOGT, GenBank: MH797025.1) and O-GlcNAcase (PvOGA, GenBank: 643 MH797026.1) were cloned into the pGEX-6P-1 vector (GE Healthcare, Boston, MA, 644 Jo urn al Pr e-p roo f 23 USA, 28-9546-48) at the BamHI site using the ClonExpress II One Step Cloning Kit 645 (Vazyme, Nanjing, China, C112). ..

    Construct:

    Article Title: Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimp.
    Article Snippet: .. 639 640 Plasmid constructs, protein expression, and purification 641 The coding sequences for the functional domains of P. vannamei O-GlcNAc transferase 642 (PvOGT, GenBank: MH797025.1) and O-GlcNAcase (PvOGA, GenBank: 643 MH797026.1) were cloned into the pGEX-6P-1 vector (GE Healthcare, Boston, MA, 644 Jo urn al Pr e-p roo f 23 USA, 28-9546-48) at the BamHI site using the ClonExpress II One Step Cloning Kit 645 (Vazyme, Nanjing, China, C112). ..

    Purification:

    Article Title: Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimp.
    Article Snippet: .. 639 640 Plasmid constructs, protein expression, and purification 641 The coding sequences for the functional domains of P. vannamei O-GlcNAc transferase 642 (PvOGT, GenBank: MH797025.1) and O-GlcNAcase (PvOGA, GenBank: 643 MH797026.1) were cloned into the pGEX-6P-1 vector (GE Healthcare, Boston, MA, 644 Jo urn al Pr e-p roo f 23 USA, 28-9546-48) at the BamHI site using the ClonExpress II One Step Cloning Kit 645 (Vazyme, Nanjing, China, C112). ..

    Functional Assay:

    Article Title: Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimp.
    Article Snippet: .. 639 640 Plasmid constructs, protein expression, and purification 641 The coding sequences for the functional domains of P. vannamei O-GlcNAc transferase 642 (PvOGT, GenBank: MH797025.1) and O-GlcNAcase (PvOGA, GenBank: 643 MH797026.1) were cloned into the pGEX-6P-1 vector (GE Healthcare, Boston, MA, 644 Jo urn al Pr e-p roo f 23 USA, 28-9546-48) at the BamHI site using the ClonExpress II One Step Cloning Kit 645 (Vazyme, Nanjing, China, C112). ..

    Cloning:

    Article Title: Hexosamine biosynthesis drives hemocyanin O-GlcNAcylation to potentiate antibacterial immunity in shrimp.
    Article Snippet: .. 639 640 Plasmid constructs, protein expression, and purification 641 The coding sequences for the functional domains of P. vannamei O-GlcNAc transferase 642 (PvOGT, GenBank: MH797025.1) and O-GlcNAcase (PvOGA, GenBank: 643 MH797026.1) were cloned into the pGEX-6P-1 vector (GE Healthcare, Boston, MA, 644 Jo urn al Pr e-p roo f 23 USA, 28-9546-48) at the BamHI site using the ClonExpress II One Step Cloning Kit 645 (Vazyme, Nanjing, China, C112). ..

    In Vitro:

    Article Title: The CCCH-type zinc-finger Pf MD3 promotes translation for malaria parasite transmission
    Article Snippet: .. For in vitro RNA-binding assays, the full length, short (aa 140-214), medium (aa 1-214) and long (aa 140-485) versions of Pf MD3 were cloned downstream of a GST-tag in the pGEX6P1 (Cytiva, 28-9546-48) vector for recombinant protein expression in bacteria, E. coli BL21(DE3)pLysS (Promega, L1195). ..

    Recombinant:

    Article Title: The CCCH-type zinc-finger Pf MD3 promotes translation for malaria parasite transmission
    Article Snippet: .. For in vitro RNA-binding assays, the full length, short (aa 140-214), medium (aa 1-214) and long (aa 140-485) versions of Pf MD3 were cloned downstream of a GST-tag in the pGEX6P1 (Cytiva, 28-9546-48) vector for recombinant protein expression in bacteria, E. coli BL21(DE3)pLysS (Promega, L1195). ..

    Bacteria:

    Article Title: The CCCH-type zinc-finger Pf MD3 promotes translation for malaria parasite transmission
    Article Snippet: .. For in vitro RNA-binding assays, the full length, short (aa 140-214), medium (aa 1-214) and long (aa 140-485) versions of Pf MD3 were cloned downstream of a GST-tag in the pGEX6P1 (Cytiva, 28-9546-48) vector for recombinant protein expression in bacteria, E. coli BL21(DE3)pLysS (Promega, L1195). ..



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