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gfp  (OriGene)


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    Structured Review

    OriGene gfp
    Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector/pCMV6-A-GFP+Mammalian+Expression+Vector/pmc10548452-1437-25-28
    Average 93 stars, based on 49 article reviews
    gfp - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: ADH1B, the adipocyte-enriched alcohol dehydrogenase, plays an essential, cell-autonomous role in human adipogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- ADH1B Protein Tech Cat# 17165- 1- AP Anti- Adiponectin Thermo Fisher Scientific Cat# MA1- 054 Anti- C/EBPα Protein Tech Cat# 18311- 1- AP Anti- FAS Cell Signaling Technology Cat# 3180 Anti- Leptin Thermo Fisher Scientific Cat# PA1- 051 Anti- Perilipin- 1 Abcam Cat# ab3526 Anti- PPARγ Protein Tech Cat# 16643- 1- AP Anti- SREBP- 1 Santa Cruz Biotechnology Cat# sc- 366 Anti- Tubulin Sigma- Aldrich Cat# T5168 Anti- rabbit- HRP Cell Signaling Technology Cat# 7074 Anti- mouse- HRP Cell Signaling Technology Cat# 7076 Biological Samples Adipose Stem Cells (ASC) Pr. .. Fève Lab at CRSA, Paris N/A Fetal calf serum Sigma- Aldrich Cat# F7524 Newborn calf serum Biosera Cat# CA- 1151500 Chemicals, Peptides, and Recombinant Proteins 2,7- dichlorodihydrofluorescein diacetate (CM- H2DCFHDA) Sigma- Aldrich Cat# C6827 3- isobutyl- 1- methyl xanthine (IBMX) Sigma- Aldrich Cat# I7018 9- cis- retinoic acid (CAS 5300- 03- 8) Santa Cruz Biotechnology Cat# sc- 205589 All- trans- retinoic acid (CAS 302- 79- 4) Sigma- Aldrich Cat# R2625 All- trans- retinol (CAS 68- 26- 8) Sigma- Aldrich Cat# R7632 DAPI Sigma- Aldrich Cat# D1306 Dexamethasone Sigma- Aldrich Cat# D4902 Fibrobast Growth Factor- 2 (FGF- 2) PeproTech Cat# 100- 18B GlutaMAX Thermo Fisher Scientific Cat# 35050061 G- 418 Sulfate Sigma- Aldrich Cat# G418- RO HEPES Thermo Fisher Scientific Cat# 15630056 Insulin Sigma- Aldrich Cat# I0516 Oil Red- O Sigma- Aldrich Cat# O0625 Paraformaldehyde Thermo Fisher Scientific Cat# J19943.K2 Penicillin/streptomycin Thermo Fisher Scientific Cat# 11548876 Rosiglitazone Sigma- Aldrich Cat# D2408 Critical Commercial Assays Human MSC Kit buffer Lonza Cat# VPE- 1001 MycoAlertTM PLUS Mycoplasma Detection Kit Lonza Cat# LT07- 701 QuantiChromTM Alcohol dehydrogenase Assay Kit BioAssay Systems Cat# DADH- 100 QuikChange II Site- directed mutagenesis kit Agilent Technologies Cat# 200523 Triglyceride Infinity KitTM Thermo Fisher Scientific Cat# TR22421 TurboFectTM Thermo Fisher Scientific Cat# R0532 Recombinant DNA lentiCRISPR v2 Addgene Cat# 52961 pCMV6- entry- GFP Origene Cat# PS100026 pCMV- ADH1B WT- Flag Origene Cat# RC205391L3 pCMV- ADH1B c.937C>T- Flag Described in Methods N/A Software and Algorithms Multi- Experiment Viewer (MeV) WebMev N/A Prism GraphPad Software N/A Web resources CADD https://cadd.gs.washington.edu CRISPOR http://crispor.tefor.net/D ow nl oa de d fr om h ttp s: //w w w .p na s. or g by 1 03 .1 39 .1 0. ..

    Alcohol Dehydrogenase Assay:

    Article Title: ADH1B, the adipocyte-enriched alcohol dehydrogenase, plays an essential, cell-autonomous role in human adipogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- ADH1B Protein Tech Cat# 17165- 1- AP Anti- Adiponectin Thermo Fisher Scientific Cat# MA1- 054 Anti- C/EBPα Protein Tech Cat# 18311- 1- AP Anti- FAS Cell Signaling Technology Cat# 3180 Anti- Leptin Thermo Fisher Scientific Cat# PA1- 051 Anti- Perilipin- 1 Abcam Cat# ab3526 Anti- PPARγ Protein Tech Cat# 16643- 1- AP Anti- SREBP- 1 Santa Cruz Biotechnology Cat# sc- 366 Anti- Tubulin Sigma- Aldrich Cat# T5168 Anti- rabbit- HRP Cell Signaling Technology Cat# 7074 Anti- mouse- HRP Cell Signaling Technology Cat# 7076 Biological Samples Adipose Stem Cells (ASC) Pr. .. Fève Lab at CRSA, Paris N/A Fetal calf serum Sigma- Aldrich Cat# F7524 Newborn calf serum Biosera Cat# CA- 1151500 Chemicals, Peptides, and Recombinant Proteins 2,7- dichlorodihydrofluorescein diacetate (CM- H2DCFHDA) Sigma- Aldrich Cat# C6827 3- isobutyl- 1- methyl xanthine (IBMX) Sigma- Aldrich Cat# I7018 9- cis- retinoic acid (CAS 5300- 03- 8) Santa Cruz Biotechnology Cat# sc- 205589 All- trans- retinoic acid (CAS 302- 79- 4) Sigma- Aldrich Cat# R2625 All- trans- retinol (CAS 68- 26- 8) Sigma- Aldrich Cat# R7632 DAPI Sigma- Aldrich Cat# D1306 Dexamethasone Sigma- Aldrich Cat# D4902 Fibrobast Growth Factor- 2 (FGF- 2) PeproTech Cat# 100- 18B GlutaMAX Thermo Fisher Scientific Cat# 35050061 G- 418 Sulfate Sigma- Aldrich Cat# G418- RO HEPES Thermo Fisher Scientific Cat# 15630056 Insulin Sigma- Aldrich Cat# I0516 Oil Red- O Sigma- Aldrich Cat# O0625 Paraformaldehyde Thermo Fisher Scientific Cat# J19943.K2 Penicillin/streptomycin Thermo Fisher Scientific Cat# 11548876 Rosiglitazone Sigma- Aldrich Cat# D2408 Critical Commercial Assays Human MSC Kit buffer Lonza Cat# VPE- 1001 MycoAlertTM PLUS Mycoplasma Detection Kit Lonza Cat# LT07- 701 QuantiChromTM Alcohol dehydrogenase Assay Kit BioAssay Systems Cat# DADH- 100 QuikChange II Site- directed mutagenesis kit Agilent Technologies Cat# 200523 Triglyceride Infinity KitTM Thermo Fisher Scientific Cat# TR22421 TurboFectTM Thermo Fisher Scientific Cat# R0532 Recombinant DNA lentiCRISPR v2 Addgene Cat# 52961 pCMV6- entry- GFP Origene Cat# PS100026 pCMV- ADH1B WT- Flag Origene Cat# RC205391L3 pCMV- ADH1B c.937C>T- Flag Described in Methods N/A Software and Algorithms Multi- Experiment Viewer (MeV) WebMev N/A Prism GraphPad Software N/A Web resources CADD https://cadd.gs.washington.edu CRISPOR http://crispor.tefor.net/D ow nl oa de d fr om h ttp s: //w w w .p na s. or g by 1 03 .1 39 .1 0. ..

    Mutagenesis:

    Article Title: ADH1B, the adipocyte-enriched alcohol dehydrogenase, plays an essential, cell-autonomous role in human adipogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- ADH1B Protein Tech Cat# 17165- 1- AP Anti- Adiponectin Thermo Fisher Scientific Cat# MA1- 054 Anti- C/EBPα Protein Tech Cat# 18311- 1- AP Anti- FAS Cell Signaling Technology Cat# 3180 Anti- Leptin Thermo Fisher Scientific Cat# PA1- 051 Anti- Perilipin- 1 Abcam Cat# ab3526 Anti- PPARγ Protein Tech Cat# 16643- 1- AP Anti- SREBP- 1 Santa Cruz Biotechnology Cat# sc- 366 Anti- Tubulin Sigma- Aldrich Cat# T5168 Anti- rabbit- HRP Cell Signaling Technology Cat# 7074 Anti- mouse- HRP Cell Signaling Technology Cat# 7076 Biological Samples Adipose Stem Cells (ASC) Pr. .. Fève Lab at CRSA, Paris N/A Fetal calf serum Sigma- Aldrich Cat# F7524 Newborn calf serum Biosera Cat# CA- 1151500 Chemicals, Peptides, and Recombinant Proteins 2,7- dichlorodihydrofluorescein diacetate (CM- H2DCFHDA) Sigma- Aldrich Cat# C6827 3- isobutyl- 1- methyl xanthine (IBMX) Sigma- Aldrich Cat# I7018 9- cis- retinoic acid (CAS 5300- 03- 8) Santa Cruz Biotechnology Cat# sc- 205589 All- trans- retinoic acid (CAS 302- 79- 4) Sigma- Aldrich Cat# R2625 All- trans- retinol (CAS 68- 26- 8) Sigma- Aldrich Cat# R7632 DAPI Sigma- Aldrich Cat# D1306 Dexamethasone Sigma- Aldrich Cat# D4902 Fibrobast Growth Factor- 2 (FGF- 2) PeproTech Cat# 100- 18B GlutaMAX Thermo Fisher Scientific Cat# 35050061 G- 418 Sulfate Sigma- Aldrich Cat# G418- RO HEPES Thermo Fisher Scientific Cat# 15630056 Insulin Sigma- Aldrich Cat# I0516 Oil Red- O Sigma- Aldrich Cat# O0625 Paraformaldehyde Thermo Fisher Scientific Cat# J19943.K2 Penicillin/streptomycin Thermo Fisher Scientific Cat# 11548876 Rosiglitazone Sigma- Aldrich Cat# D2408 Critical Commercial Assays Human MSC Kit buffer Lonza Cat# VPE- 1001 MycoAlertTM PLUS Mycoplasma Detection Kit Lonza Cat# LT07- 701 QuantiChromTM Alcohol dehydrogenase Assay Kit BioAssay Systems Cat# DADH- 100 QuikChange II Site- directed mutagenesis kit Agilent Technologies Cat# 200523 Triglyceride Infinity KitTM Thermo Fisher Scientific Cat# TR22421 TurboFectTM Thermo Fisher Scientific Cat# R0532 Recombinant DNA lentiCRISPR v2 Addgene Cat# 52961 pCMV6- entry- GFP Origene Cat# PS100026 pCMV- ADH1B WT- Flag Origene Cat# RC205391L3 pCMV- ADH1B c.937C>T- Flag Described in Methods N/A Software and Algorithms Multi- Experiment Viewer (MeV) WebMev N/A Prism GraphPad Software N/A Web resources CADD https://cadd.gs.washington.edu CRISPOR http://crispor.tefor.net/D ow nl oa de d fr om h ttp s: //w w w .p na s. or g by 1 03 .1 39 .1 0. ..

    Software:

    Article Title: ADH1B, the adipocyte-enriched alcohol dehydrogenase, plays an essential, cell-autonomous role in human adipogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- ADH1B Protein Tech Cat# 17165- 1- AP Anti- Adiponectin Thermo Fisher Scientific Cat# MA1- 054 Anti- C/EBPα Protein Tech Cat# 18311- 1- AP Anti- FAS Cell Signaling Technology Cat# 3180 Anti- Leptin Thermo Fisher Scientific Cat# PA1- 051 Anti- Perilipin- 1 Abcam Cat# ab3526 Anti- PPARγ Protein Tech Cat# 16643- 1- AP Anti- SREBP- 1 Santa Cruz Biotechnology Cat# sc- 366 Anti- Tubulin Sigma- Aldrich Cat# T5168 Anti- rabbit- HRP Cell Signaling Technology Cat# 7074 Anti- mouse- HRP Cell Signaling Technology Cat# 7076 Biological Samples Adipose Stem Cells (ASC) Pr. .. Fève Lab at CRSA, Paris N/A Fetal calf serum Sigma- Aldrich Cat# F7524 Newborn calf serum Biosera Cat# CA- 1151500 Chemicals, Peptides, and Recombinant Proteins 2,7- dichlorodihydrofluorescein diacetate (CM- H2DCFHDA) Sigma- Aldrich Cat# C6827 3- isobutyl- 1- methyl xanthine (IBMX) Sigma- Aldrich Cat# I7018 9- cis- retinoic acid (CAS 5300- 03- 8) Santa Cruz Biotechnology Cat# sc- 205589 All- trans- retinoic acid (CAS 302- 79- 4) Sigma- Aldrich Cat# R2625 All- trans- retinol (CAS 68- 26- 8) Sigma- Aldrich Cat# R7632 DAPI Sigma- Aldrich Cat# D1306 Dexamethasone Sigma- Aldrich Cat# D4902 Fibrobast Growth Factor- 2 (FGF- 2) PeproTech Cat# 100- 18B GlutaMAX Thermo Fisher Scientific Cat# 35050061 G- 418 Sulfate Sigma- Aldrich Cat# G418- RO HEPES Thermo Fisher Scientific Cat# 15630056 Insulin Sigma- Aldrich Cat# I0516 Oil Red- O Sigma- Aldrich Cat# O0625 Paraformaldehyde Thermo Fisher Scientific Cat# J19943.K2 Penicillin/streptomycin Thermo Fisher Scientific Cat# 11548876 Rosiglitazone Sigma- Aldrich Cat# D2408 Critical Commercial Assays Human MSC Kit buffer Lonza Cat# VPE- 1001 MycoAlertTM PLUS Mycoplasma Detection Kit Lonza Cat# LT07- 701 QuantiChromTM Alcohol dehydrogenase Assay Kit BioAssay Systems Cat# DADH- 100 QuikChange II Site- directed mutagenesis kit Agilent Technologies Cat# 200523 Triglyceride Infinity KitTM Thermo Fisher Scientific Cat# TR22421 TurboFectTM Thermo Fisher Scientific Cat# R0532 Recombinant DNA lentiCRISPR v2 Addgene Cat# 52961 pCMV6- entry- GFP Origene Cat# PS100026 pCMV- ADH1B WT- Flag Origene Cat# RC205391L3 pCMV- ADH1B c.937C>T- Flag Described in Methods N/A Software and Algorithms Multi- Experiment Viewer (MeV) WebMev N/A Prism GraphPad Software N/A Web resources CADD https://cadd.gs.washington.edu CRISPOR http://crispor.tefor.net/D ow nl oa de d fr om h ttp s: //w w w .p na s. or g by 1 03 .1 39 .1 0. ..

    Sequencing:

    Article Title: Sonic Hedgehog Determines Early Retinal Development and Adjusts Eyeball Architecture
    Article Snippet: .. Sequencing analysis confirmed that the inserts bear the appropriate sequence. pCMV6-hSHH with C-terminal tGFP and pCMV6-GFP were purchased from OriGene (Rockville, MD, USA). ..

    Amplification:

    Article Title: Downregulation of Gene Expression by Alpha Satellite Transcripts
    Article Snippet: .. The alpha satellite 171 bp long monomer was amplified by PCR from MJ90-hTERT genomic DNA and cloned into the pCMV6-A-GFP plasmid vector (OriGene Technologies, Inc., Rockville, MD, USA). ..

    Polymerase Chain Reaction:

    Article Title: Downregulation of Gene Expression by Alpha Satellite Transcripts
    Article Snippet: .. The alpha satellite 171 bp long monomer was amplified by PCR from MJ90-hTERT genomic DNA and cloned into the pCMV6-A-GFP plasmid vector (OriGene Technologies, Inc., Rockville, MD, USA). ..

    Clone Assay:

    Article Title: Downregulation of Gene Expression by Alpha Satellite Transcripts
    Article Snippet: .. The alpha satellite 171 bp long monomer was amplified by PCR from MJ90-hTERT genomic DNA and cloned into the pCMV6-A-GFP plasmid vector (OriGene Technologies, Inc., Rockville, MD, USA). ..

    Article Title: CTCF-associated chromatin changes between HOXD1 and HOXD8 contribute to TNBC progression.
    Article Snippet: These BC cell lines were maintained in Dulbecco’s Modified Eagle Medium (DMEM: LM001-05, WelGENE Inc.) supplemented with 10% fetal bovine serum, 100mg/ml penicillin, 10mg/ml streptomycin sulfate, and 25 μg/ml amphotericin B (LS204-01, WelGENE Inc.) at 37 °C in 5% CO2. .. For overexpression studies, full-length HOXD1 and HOXD8 cDNAs were cloned into a pCMV6 expression vector (PS100026, OriGene). .. MDA-MB-231 and MDA-MB-468 cells were transfected with a plasmid DNA using Attractene eagent (301005, Qiagen).

    Plasmid Preparation:

    Article Title: Downregulation of Gene Expression by Alpha Satellite Transcripts
    Article Snippet: .. The alpha satellite 171 bp long monomer was amplified by PCR from MJ90-hTERT genomic DNA and cloned into the pCMV6-A-GFP plasmid vector (OriGene Technologies, Inc., Rockville, MD, USA). ..

    Article Title: CTCF-associated chromatin changes between HOXD1 and HOXD8 contribute to TNBC progression.
    Article Snippet: These BC cell lines were maintained in Dulbecco’s Modified Eagle Medium (DMEM: LM001-05, WelGENE Inc.) supplemented with 10% fetal bovine serum, 100mg/ml penicillin, 10mg/ml streptomycin sulfate, and 25 μg/ml amphotericin B (LS204-01, WelGENE Inc.) at 37 °C in 5% CO2. .. For overexpression studies, full-length HOXD1 and HOXD8 cDNAs were cloned into a pCMV6 expression vector (PS100026, OriGene). .. MDA-MB-231 and MDA-MB-468 cells were transfected with a plasmid DNA using Attractene eagent (301005, Qiagen).

    Article Title: In vivo conformational space and defects of misfolded CFTR variants by covalent protein painting.
    Article Snippet: .. K273A CFTR was generated using the Quick change method (Quiagen) in the pCMV6-ac-CFTR-GFP plasmid (Origene). .. Cell culture, plasmids and point mutations CFBE andHBE cells were grown in A-MEM supplementedwith 10% FBS, 1% Penicillin-Streptomycin (GibCo, Carlsbad, CA) and appropriate antibiotics at 37 °C, 5% CO2.

    other:

    Article Title: Novel PEI-aldehyde conjugates for gene delivery: Promoting chondrogenic differentiation in human mesenchymal stem cells
    Article Snippet: The Label IT Nucleic Acid Labeling kit Cy3 was obtained from Mirus Bio (Madison, WI, USA).

    Over Expression:

    Article Title: CTCF-associated chromatin changes between HOXD1 and HOXD8 contribute to TNBC progression.
    Article Snippet: These BC cell lines were maintained in Dulbecco’s Modified Eagle Medium (DMEM: LM001-05, WelGENE Inc.) supplemented with 10% fetal bovine serum, 100mg/ml penicillin, 10mg/ml streptomycin sulfate, and 25 μg/ml amphotericin B (LS204-01, WelGENE Inc.) at 37 °C in 5% CO2. .. For overexpression studies, full-length HOXD1 and HOXD8 cDNAs were cloned into a pCMV6 expression vector (PS100026, OriGene). .. MDA-MB-231 and MDA-MB-468 cells were transfected with a plasmid DNA using Attractene eagent (301005, Qiagen).

    Expressing:

    Article Title: CTCF-associated chromatin changes between HOXD1 and HOXD8 contribute to TNBC progression.
    Article Snippet: These BC cell lines were maintained in Dulbecco’s Modified Eagle Medium (DMEM: LM001-05, WelGENE Inc.) supplemented with 10% fetal bovine serum, 100mg/ml penicillin, 10mg/ml streptomycin sulfate, and 25 μg/ml amphotericin B (LS204-01, WelGENE Inc.) at 37 °C in 5% CO2. .. For overexpression studies, full-length HOXD1 and HOXD8 cDNAs were cloned into a pCMV6 expression vector (PS100026, OriGene). .. MDA-MB-231 and MDA-MB-468 cells were transfected with a plasmid DNA using Attractene eagent (301005, Qiagen).

    Generated:

    Article Title: In vivo conformational space and defects of misfolded CFTR variants by covalent protein painting.
    Article Snippet: .. K273A CFTR was generated using the Quick change method (Quiagen) in the pCMV6-ac-CFTR-GFP plasmid (Origene). .. Cell culture, plasmids and point mutations CFBE andHBE cells were grown in A-MEM supplementedwith 10% FBS, 1% Penicillin-Streptomycin (GibCo, Carlsbad, CA) and appropriate antibiotics at 37 °C, 5% CO2.



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    Image Search Results


    Journal: iScience

    Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease

    doi: 10.1016/j.isci.2026.117642

    Figure Lengend Snippet:

    Article Snippet: The cDNAs encoding the extracellular domains of the HLA-DR3 α- and β-chains were separately cloned into the pHLsec expression vector (Addgene plasmid #99845).

    Techniques: Isolation, Cell Isolation, DNA Extraction, Quantitation Assay, Plasmid Preparation, Extraction, Recombinant, Expressing, Software