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pcmv6 a puro  (OriGene)


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    Structured Review

    OriGene pcmv6 a puro
    Pcmv6 A Puro, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector/pCMV6-A-Puro+Mammalian+Expression+Vector/pmc07170992__mmc1-55-35-36
    Average 93 stars, based on 37 article reviews
    pcmv6 a puro - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Over Expression:

    Article Title: Deletion of NFIA Leads to Activation of S100A7 and Inflammatory Response‐Induced Apoptosis of Keratinocytes in Oral Lichen Planus Progression
    Article Snippet: .. On the second day, the overexpression lentiviral vector (> 10 9 TU/ml, VectorBuilder) for S100A7 and NFIA human genes, S100A7 human gene shRNA lentiviral vector (> 10 9 TU/mL, VectorBuilder), and pCMV6‐A‐Puro empty vector (PS100025, OriGene Technologies; Beijing, China) were used to infect cells at 37°C. ..

    Plasmid Preparation:

    Article Title: Deletion of NFIA Leads to Activation of S100A7 and Inflammatory Response‐Induced Apoptosis of Keratinocytes in Oral Lichen Planus Progression
    Article Snippet: .. On the second day, the overexpression lentiviral vector (> 10 9 TU/ml, VectorBuilder) for S100A7 and NFIA human genes, S100A7 human gene shRNA lentiviral vector (> 10 9 TU/mL, VectorBuilder), and pCMV6‐A‐Puro empty vector (PS100025, OriGene Technologies; Beijing, China) were used to infect cells at 37°C. ..

    Article Title: Cell-type-specific dysregulation of mitochondrial calcium signaling in Alzheimer’s disease
    Article Snippet: .. APPswe/F/L plasmid was purchased from Vecto Builder (Cat# VB220324-1227aqs) and pCMV6-A-Puro (Cat# PS100025 ) was purchased from Origene. .. Plasmid transfection was performed using FUGENE transfection reagent (Promega, Cat# E5911) according to the manufacturer’s protocol.

    Article Title: Effect of LOXL2 on metastasis through remodeling of the cell surface matrix in non-small cell lung cancer cells.
    Article Snippet: Lung cancer is the prominent cause of cancer-associated death primarily because of distant metastatic disease.. The metastatic potential of non-small cell lung cancer (NSCLC) is associated with tumor cell aggregation.. However, the systemic mechanotransduction mechanism by which tumor cells dynamically aggregate and disseminate is poorly understood, especially in NSCLC.

    Article Title: Synthesis, radiolabeling, and biological evaluation of methyl 6-deoxy-6-[ 18 F]fluoro-4-thio-α- d -maltotrioside as a positron emission tomography bacterial imaging agent
    Article Snippet: .. The mammalian expression vector, pCMV6-A-puro plasmid (PS100025), was obtained from OriGene. .. Restriction enzymes, ligation enzymes, and T4 polymerase were obtained from New England Biolabs.

    shRNA:

    Article Title: Deletion of NFIA Leads to Activation of S100A7 and Inflammatory Response‐Induced Apoptosis of Keratinocytes in Oral Lichen Planus Progression
    Article Snippet: .. On the second day, the overexpression lentiviral vector (> 10 9 TU/ml, VectorBuilder) for S100A7 and NFIA human genes, S100A7 human gene shRNA lentiviral vector (> 10 9 TU/mL, VectorBuilder), and pCMV6‐A‐Puro empty vector (PS100025, OriGene Technologies; Beijing, China) were used to infect cells at 37°C. ..

    Expressing:

    Article Title: A distinct isoform of lymphoid enhancer binding factor 1 (LEF1) epigenetically restricts EBV reactivation to maintain viral latency.
    Article Snippet: .. pCMV6-A-puro (ORIGENE PS100025) vector was used for expression of LEF1 variants. .. LEF1 variant 3 (NM_001130714) and variant 4 (NM_001166119) encoding DNA fragments were shuttled from pCMV6-entry vector-neo (PS100001) by digestion with BamH I and Pme I restriction enzymes and introduced at the same restriction sites in pCMV6-A-puro.

    Article Title: Effect of LOXL2 on metastasis through remodeling of the cell surface matrix in non-small cell lung cancer cells.
    Article Snippet: Lung cancer is the prominent cause of cancer-associated death primarily because of distant metastatic disease.. The metastatic potential of non-small cell lung cancer (NSCLC) is associated with tumor cell aggregation.. However, the systemic mechanotransduction mechanism by which tumor cells dynamically aggregate and disseminate is poorly understood, especially in NSCLC.

    Article Title: A distinct isoform of lymphoid enhancer binding factor 1 (LEF1) epigenetically restricts EBV reactivation to maintain viral latency
    Article Snippet: .. pCMV6-A-puro (ORIGENE PS100025) vector was used for expression of LEF1 variants. .. LEF1 variant 3 (NM_001130714) and variant 4 (NM_001166119) encoding DNA fragments were shuttled from pCMV6-entry vector-neo (PS100001) by digestion with BamH I and Pme I restriction enzymes and introduced at the same restriction sites in pCMV6-A-puro.

    Article Title: Synthesis, radiolabeling, and biological evaluation of methyl 6-deoxy-6-[ 18 F]fluoro-4-thio-α- d -maltotrioside as a positron emission tomography bacterial imaging agent
    Article Snippet: .. The mammalian expression vector, pCMV6-A-puro plasmid (PS100025), was obtained from OriGene. .. Restriction enzymes, ligation enzymes, and T4 polymerase were obtained from New England Biolabs.

    Transfection:

    Article Title: Effect of LOXL2 on metastasis through remodeling of the cell surface matrix in non-small cell lung cancer cells.
    Article Snippet: Lung cancer is the prominent cause of cancer-associated death primarily because of distant metastatic disease.. The metastatic potential of non-small cell lung cancer (NSCLC) is associated with tumor cell aggregation.. However, the systemic mechanotransduction mechanism by which tumor cells dynamically aggregate and disseminate is poorly understood, especially in NSCLC.

    Article Title: p53-Independent Cell Cycle and Erythroid Differentiation Defects in Murine Embryonic Stem Cells Haploinsufficient for Diamond Blackfan Anemia-Proteins: RPS19 versus RPL5
    Article Snippet: .. The Rpl5 mutant cell line D050B12 was transfected, using FuGene HD (Promega), with a pCMV6-A-Puro vector containing wild type Rpl5 cDNA and a puromycin resistance gene (Origene) to establish a stably transfected clone overexpressing Rpl5. .. Twenty-four hours prior to primary differentiation, small interfering RNA (siRNA) targeting p53, (Dharmacon; see ) were transiently transfected into pre-differentiation cell cultures, using DharmaFect 1 (Dharmacon) transfection reagent according to the manufacturer's specifications.

    Control:

    Article Title: Effect of LOXL2 on metastasis through remodeling of the cell surface matrix in non-small cell lung cancer cells.
    Article Snippet: Lung cancer is the prominent cause of cancer-associated death primarily because of distant metastatic disease.. The metastatic potential of non-small cell lung cancer (NSCLC) is associated with tumor cell aggregation.. However, the systemic mechanotransduction mechanism by which tumor cells dynamically aggregate and disseminate is poorly understood, especially in NSCLC.

    Mutagenesis:

    Article Title: p53-Independent Cell Cycle and Erythroid Differentiation Defects in Murine Embryonic Stem Cells Haploinsufficient for Diamond Blackfan Anemia-Proteins: RPS19 versus RPL5
    Article Snippet: .. The Rpl5 mutant cell line D050B12 was transfected, using FuGene HD (Promega), with a pCMV6-A-Puro vector containing wild type Rpl5 cDNA and a puromycin resistance gene (Origene) to establish a stably transfected clone overexpressing Rpl5. .. Twenty-four hours prior to primary differentiation, small interfering RNA (siRNA) targeting p53, (Dharmacon; see ) were transiently transfected into pre-differentiation cell cultures, using DharmaFect 1 (Dharmacon) transfection reagent according to the manufacturer's specifications.

    Stable Transfection:

    Article Title: p53-Independent Cell Cycle and Erythroid Differentiation Defects in Murine Embryonic Stem Cells Haploinsufficient for Diamond Blackfan Anemia-Proteins: RPS19 versus RPL5
    Article Snippet: .. The Rpl5 mutant cell line D050B12 was transfected, using FuGene HD (Promega), with a pCMV6-A-Puro vector containing wild type Rpl5 cDNA and a puromycin resistance gene (Origene) to establish a stably transfected clone overexpressing Rpl5. .. Twenty-four hours prior to primary differentiation, small interfering RNA (siRNA) targeting p53, (Dharmacon; see ) were transiently transfected into pre-differentiation cell cultures, using DharmaFect 1 (Dharmacon) transfection reagent according to the manufacturer's specifications.



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    Image Search Results


    Journal: iScience

    Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease

    doi: 10.1016/j.isci.2026.117642

    Figure Lengend Snippet:

    Article Snippet: The cDNAs encoding the extracellular domains of the HLA-DR3 α- and β-chains were separately cloned into the pHLsec expression vector (Addgene plasmid #99845).

    Techniques: Isolation, Cell Isolation, DNA Extraction, Quantitation Assay, Plasmid Preparation, Extraction, Recombinant, Expressing, Software