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plenti c myc ddk p2apuro  (OriGene)


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    OriGene plenti c myc ddk p2apuro
    Plenti C Myc Ddk P2apuro, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector/pLenti-C-Myc-DDK-P2A-Puro+Lentiviral+Gene+Expression+Vector/pm42251161-372-14-15
    Average 96 stars, based on 79 article reviews
    plenti c myc ddk p2apuro - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Luciferase:

    Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
    Article Snippet: pLKO.1 control (further termed “shCTRL”) was a gift from William Hahn (Addgene plasmid, 42559; RRID: Addgene_42559). .. COL6A2 and luciferase control (oeCTRL) were subcloned into pLenti-C-Myc-DDK-P2A-Puro expression vector backbone (PS100092, OriGene Technologies). .. COL6A2_pCSdest was a gift from Roger Reeves (Addgene plasmid, 53903; RRID: Addgene_53903) .

    Control:

    Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
    Article Snippet: pLKO.1 control (further termed “shCTRL”) was a gift from William Hahn (Addgene plasmid, 42559; RRID: Addgene_42559). .. COL6A2 and luciferase control (oeCTRL) were subcloned into pLenti-C-Myc-DDK-P2A-Puro expression vector backbone (PS100092, OriGene Technologies). .. COL6A2_pCSdest was a gift from Roger Reeves (Addgene plasmid, 53903; RRID: Addgene_53903) .

    Article Title: SOX9 and SEMA7A regulate cell plasticity in the postpartum mammary gland with implications for breast cancer
    Article Snippet: To generate SEMA7A OE cell lines, plasmids containing a SEMA7A-ORF Clone insert (Origene, #MR209876L3, pLenti-C-Myc-DDK-P2A-puro backbone) were packaged into lentivirus at the CU Functional Genomics Shared Resource. .. The same backbone without an ORF insert was used as an empty vector control (Origene, #PS100092). .. HC-11 and EpH4 cells were grown to 70% confluence and incubated with a 1:1 ratio of cell maintenance media and viral particles with 8 u g/mL polybrene (Sigma-Aldrich, #TR-1003).

    Article Title: Discovery of BEND4 as a novel single-gene prognostic marker and therapeutic target for adverse AML.
    Article Snippet: .. For BEND4 overexpression, pLenti-c-Myc-DDK-P2A-Puro-BEND4 (Origene, CAT: RC212072L3) was used, and pLenti-c-MYC-DDK-Puro-GFP (Addgene, 123299) or pLenti-C-Myc-DDK-P2APuro (Origene, CAT: PS100092) were used as vector control. .. For BEND4 knockdown, 3 different shRNAs with TRC clone ID TRCN0000245693, TRCN0000245694, and TRCN0000245695 were purchased from Sigma and pLKO.1-puro Empty Vector Control (Sigma, #SHC001) was used as vector control.

    Article Title: Resistance to neoadjuvant talazoparib in triple-negative breast cancer by BRN2-induced ATR/STAT3 pathways or SHLD2 subclone expansion
    Article Snippet: Fresh media were added every 2 to 3 d. Organoid cells were recovered from the matrigel matrix by incubating them with cell recovery solution (Corning, Cat. No. 354253) on ice for approximately 30 min, with frequent pipetting. .. For BRN2 overproduction studies, control lentivirus (Cat. No. PS100092 ) and lentiviruses encoding human BRN2 (Cat. No. RC205330) were purchased from OriGene. .. For knockdown experiments, lentivirus containing BRN2 shRNAs (Cat. No. TRCN0000019329, shRNA #1; and Cat. No. TRCN0000019332, shRNA #2) and a nontargeting control (Cat. No. SHC007) were obtained from Sigma Chemical Company.

    Article Title: Resistance to neoadjuvant talazoparib in triple-negative breast cancer by BRN2-induced ATR/STAT3 pathways or SHLD2 subclone expansion.
    Article Snippet: Fresh media were added every 2 to 3 d. Organoid cells were recovered from the matrigel matrix by incubating them with cell recovery solution (Corning, Cat. No. 354253) on ice for approximately 30 min, with frequent pipetting. .. For BRN2 overproduction studies, control lentivirus (Cat. No. PS100092) and lentiviruses encoding human BRN2 (Cat. No. RC205330) were purchased from OriGene. .. For knockdown experiments, lentivirus containing BRN2 shRNAs (Cat. No. TRCN0000019329, shRNA #1; and Cat. No. TRCN0000019332, shRNA #2) and a nontargeting control (Cat. No. SHC007) were obtained from Sigma Chemical Company.

    Article Title: CCR2 is a potential biomarker and therapeutic target in Group 3 medulloblastoma
    Article Snippet: A lentiviral plasmid encoding the open reading frame of CCR2 variant B (Origene, RC225538L3) was used in this process. .. An empty vector plasmid (Origene, PS100092) was used as the control for transduction. ..

    Expressing:

    Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
    Article Snippet: pLKO.1 control (further termed “shCTRL”) was a gift from William Hahn (Addgene plasmid, 42559; RRID: Addgene_42559). .. COL6A2 and luciferase control (oeCTRL) were subcloned into pLenti-C-Myc-DDK-P2A-Puro expression vector backbone (PS100092, OriGene Technologies). .. COL6A2_pCSdest was a gift from Roger Reeves (Addgene plasmid, 53903; RRID: Addgene_53903) .

    Article Title: Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics
    Article Snippet: Cells were sorted into 96-well plates (Sarstedt) and grown to generate stable cell lines, as shown above. .. To rescue human ETFDH expression in HCT-116 cells, ETFDH( NM_004453 ) Human Tagged ORF was cloned into pLenti-ETFDH-C-Myc-DDK-P2A-Puro plasmid according to the manufacturer’s instructions (Origene) to generate the resulting plasmid, pLenti-ETFDH-C-Myc-DDK-P2A-Puro. .. To re-express mutant ETFDH (Y304A, G306E), WT ETFDH, or ORF-Stuffer lentiviral plasmids conferring hygromycin B resistance were designed using VectorBuilder (VB250613-1532mdm, VB250613-1530fzr, and VB900172-2887sfj, respectively).

    Plasmid Preparation:

    Article Title: Fibroblast-derived Collagen VI shapes the structure and function of the tumor-immune microenvironment in clear cell renal cell carcinoma
    Article Snippet: pLKO.1 control (further termed “shCTRL”) was a gift from William Hahn (Addgene plasmid, 42559; RRID: Addgene_42559). .. COL6A2 and luciferase control (oeCTRL) were subcloned into pLenti-C-Myc-DDK-P2A-Puro expression vector backbone (PS100092, OriGene Technologies). .. COL6A2_pCSdest was a gift from Roger Reeves (Addgene plasmid, 53903; RRID: Addgene_53903) .

    Article Title: Discovery of BEND4 as a novel single-gene prognostic marker and therapeutic target for adverse AML.
    Article Snippet: .. For BEND4 overexpression, pLenti-c-Myc-DDK-P2A-Puro-BEND4 (Origene, CAT: RC212072L3) was used, and pLenti-c-MYC-DDK-Puro-GFP (Addgene, 123299) or pLenti-C-Myc-DDK-P2APuro (Origene, CAT: PS100092) were used as vector control. .. For BEND4 knockdown, 3 different shRNAs with TRC clone ID TRCN0000245693, TRCN0000245694, and TRCN0000245695 were purchased from Sigma and pLKO.1-puro Empty Vector Control (Sigma, #SHC001) was used as vector control.

    Article Title: CCR2 is a potential biomarker and therapeutic target in Group 3 medulloblastoma
    Article Snippet: A lentiviral plasmid encoding the open reading frame of CCR2 variant B (Origene, RC225538L3) was used in this process. .. An empty vector plasmid (Origene, PS100092) was used as the control for transduction. ..

    Article Title: Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics
    Article Snippet: Cells were sorted into 96-well plates (Sarstedt) and grown to generate stable cell lines, as shown above. .. To rescue human ETFDH expression in HCT-116 cells, ETFDH( NM_004453 ) Human Tagged ORF was cloned into pLenti-ETFDH-C-Myc-DDK-P2A-Puro plasmid according to the manufacturer’s instructions (Origene) to generate the resulting plasmid, pLenti-ETFDH-C-Myc-DDK-P2A-Puro. .. To re-express mutant ETFDH (Y304A, G306E), WT ETFDH, or ORF-Stuffer lentiviral plasmids conferring hygromycin B resistance were designed using VectorBuilder (VB250613-1532mdm, VB250613-1530fzr, and VB900172-2887sfj, respectively).

    Over Expression:

    Article Title: Discovery of BEND4 as a novel single-gene prognostic marker and therapeutic target for adverse AML.
    Article Snippet: .. For BEND4 overexpression, pLenti-c-Myc-DDK-P2A-Puro-BEND4 (Origene, CAT: RC212072L3) was used, and pLenti-c-MYC-DDK-Puro-GFP (Addgene, 123299) or pLenti-C-Myc-DDK-P2APuro (Origene, CAT: PS100092) were used as vector control. .. For BEND4 knockdown, 3 different shRNAs with TRC clone ID TRCN0000245693, TRCN0000245694, and TRCN0000245695 were purchased from Sigma and pLKO.1-puro Empty Vector Control (Sigma, #SHC001) was used as vector control.

    other:

    Article Title: In Vitro characterization of narciclasine: solubility, metabolic stability, and P-glycoprotein substrate status
    Article Snippet: The cells were then co-transfected with either the lentiviral ABCB1 (RC216080L3) plasmid or the control ( PS100092 ) plasmid, along with the Lenti-vpak Packaging kit (OriGene).

    Transduction:

    Article Title: CCR2 is a potential biomarker and therapeutic target in Group 3 medulloblastoma
    Article Snippet: A lentiviral plasmid encoding the open reading frame of CCR2 variant B (Origene, RC225538L3) was used in this process. .. An empty vector plasmid (Origene, PS100092) was used as the control for transduction. ..

    Clone Assay:

    Article Title: Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics
    Article Snippet: Cells were sorted into 96-well plates (Sarstedt) and grown to generate stable cell lines, as shown above. .. To rescue human ETFDH expression in HCT-116 cells, ETFDH( NM_004453 ) Human Tagged ORF was cloned into pLenti-ETFDH-C-Myc-DDK-P2A-Puro plasmid according to the manufacturer’s instructions (Origene) to generate the resulting plasmid, pLenti-ETFDH-C-Myc-DDK-P2A-Puro. .. To re-express mutant ETFDH (Y304A, G306E), WT ETFDH, or ORF-Stuffer lentiviral plasmids conferring hygromycin B resistance were designed using VectorBuilder (VB250613-1532mdm, VB250613-1530fzr, and VB900172-2887sfj, respectively).



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    Image Search Results


    Journal: iScience

    Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease

    doi: 10.1016/j.isci.2026.117642

    Figure Lengend Snippet:

    Article Snippet: The cDNAs encoding the extracellular domains of the HLA-DR3 α- and β-chains were separately cloned into the pHLsec expression vector (Addgene plasmid #99845).

    Techniques: Isolation, Cell Isolation, DNA Extraction, Quantitation Assay, Plasmid Preparation, Extraction, Recombinant, Expressing, Software