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codon optimised and synthesised sequence  (GenScript corporation)

 
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    GenScript corporation codon optimised and synthesised sequence
    Codon Optimised And Synthesised Sequence, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector+pgs21a/codon+optimised+target+gene+sequence/pmc03463603-39-3-7
    Average 90 stars, based on 1 article reviews
    codon optimised and synthesised sequence - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Plasmepsin X activates the PCRCR complex of Plasmodium falciparum by processing PfRh5 for erythrocyte invasion.
    Article Snippet: The HR plasmids assembled in a modified p1.2 plasmid encoding WR99210 resistance, were made in three steps, with 5’ and 3’ flanks (~500 bp upstream or downstream from the guide sequence) amplified from 3D7 genomic DNA and a codon-optimised target gene sequence (Genscript) cloned downstream of the 5’ flank.

    Article Title: Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling.
    Article Snippet: Protein expression and purification The codon optimised coding sequences (CDSs) of the wild-type, truncations, and point-mutants of human MuvB and B-MYB proteins were obtained from Genscript or GeneArt (Thermo Fischer Scientific) and cloned into pFASTBAC-1 or pACEBAC-1 vector using standardmethods.

    Article Title: PCRCR complex is essential for invasion of human erythrocytes by Plasmodium falciparum
    Article Snippet: The HDR plasmids assembled in a modified p1.2 plasmid encoding WR99210 resistance, were made in either 3 steps, with 5’ and 3’ flanks (~500 bp upstream or downstream from the guide sequence) amplified from 3D7 genomic DNA and a codon-optimised target gene sequence (Genscript) cloned downstream of the 5’ flank or in two steps, where the 5’ flank was synthesized and fused to the codon-optimised gene sequence (in the case of CyRPA).

    Article Title: Global shape of SvGT, a metal-dependent bacteriocin modifying S/O-HexNActransferase from actinobacteria: c -terminal dimerization modulates the function of this GT.
    Article Snippet: Here, we describe hitherto unknown shape-function of S/O-HexNActransferase SvGT (ORF AQF52_ 3101) instrumental in glycosylation of bacteriocin SvC (ORF AQF52_3099) in Streptomyces venezuelae ATCC 15439.. Data from gel filtration, mass spectrometry, analytical ultracentrifugation, and Small Angle X-ray Scattering (SAXS), experiments confirmed elongated dimeric shape in solution for SvGT protein.. Enzyme assays confirmed the dependence of SvGT on the availability of Mg ions to be functionally activated.

    Synthesized:

    Article Title: NK-92MI Cells Engineered with Anti-claudin-6 Chimeric Antigen Receptors in Immunotherapy for Ovarian Cancer
    Article Snippet: .. The optimized and synthesized codon sequences (GenScript) were cloned into pUC57. .. The CLDN6-CAR 1 , CLDN6-CAR 2 , and CD19-CAR cassettes were generated by gene synthesis and cloned into the lentiviral vector pWPXLd-2A-eGFP by Genscript Co., Ltd. (Nanjing, China).

    Clone Assay:

    Article Title: NK-92MI Cells Engineered with Anti-claudin-6 Chimeric Antigen Receptors in Immunotherapy for Ovarian Cancer
    Article Snippet: .. The optimized and synthesized codon sequences (GenScript) were cloned into pUC57. .. The CLDN6-CAR 1 , CLDN6-CAR 2 , and CD19-CAR cassettes were generated by gene synthesis and cloned into the lentiviral vector pWPXLd-2A-eGFP by Genscript Co., Ltd. (Nanjing, China).

    Article Title: Cytochrome P450 5208A3 is a promiscuous xenobiotic biotransforming enzyme in Cunninghamella elegans.
    Article Snippet: EasySelectTM Pichia Expression Kit (InvitrogenTM), EZ-RunTM Prestained Rec Protein Ladder and ZeocinTM (Thermo Fisher Scientific) were acquired from Fisher Scientific (Dublin, Ireland). .. The gene constructs of the codon optimised (OptimumGeneTM) sequences were synthesised by Genscript Biotech (Netherlands) for CYP5208A3, CYP5313D1, CPR_A, CPR_B, and CPR_C and cloned in pPICZ A with KpnI and XhoI restriction sites and using E. coli DH5α as the host. ..

    Construct:

    Article Title: Cytochrome P450 5208A3 is a promiscuous xenobiotic biotransforming enzyme in Cunninghamella elegans.
    Article Snippet: EasySelectTM Pichia Expression Kit (InvitrogenTM), EZ-RunTM Prestained Rec Protein Ladder and ZeocinTM (Thermo Fisher Scientific) were acquired from Fisher Scientific (Dublin, Ireland). .. The gene constructs of the codon optimised (OptimumGeneTM) sequences were synthesised by Genscript Biotech (Netherlands) for CYP5208A3, CYP5313D1, CPR_A, CPR_B, and CPR_C and cloned in pPICZ A with KpnI and XhoI restriction sites and using E. coli DH5α as the host. ..



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    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
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    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
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    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

    A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, Recombinant, Plasmid Preparation

    A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

    Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

    A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Journal:

    Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

    doi: 10.1016/j.pep.2007.03.017

    Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

    Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

    Techniques: Expressing, Recombinant, Plasmid Preparation