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GenScript corporation expression vector pgs21a
Expression Vector Pgs21a, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pgs21a/pgs+21a/pmc03225368-39-6-9
Average 90 stars, based on 1 article reviews
expression vector pgs21a - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Binding Assay:

Article Title: A p.N92K variant of the GTPase RAC3 disrupts cortical neuron migration and axon elongation.
Article Snippet: .. These kinase mutants were 358 subcloned into a pCAG-Flag vector. pGS21a vectors (GenScript, Piscataway, NJ) containing the RAC-359 binding region (RBR) of PAK1 (amino acids 67-150), MLK2 (amino acids 401-550), or Rho-kinase 1 360 (amino acids 67-150) were prepared as described previously (22, 36). ..

Polymerase Chain Reaction:

Article Title: Surface immobilization and properties optimization of phage hydrolase against Gram-negative bacteria.
Article Snippet: Immobilized hydrolase not only reduces the production of antibiotic-resistant bacteria, but also effectively improves the stability of hydrolase in external use.. In this study, phage hydrolase LysSSE1 against Gram-negative bacteria were surface immobilized and optimized for their bactericidal activity.. Different anti-pathogen surface materials were prepared, where LysSSE1 was immobilized on the glass surface with a silica-affinity peptide and into which different peptide linkers were introduced.

Article Title: Pathophysiological significance of the p.E31G variant in RAC1 responsible for a neurodevelopmental disorder with microcephaly.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Amplification:

Article Title: Surface immobilization and properties optimization of phage hydrolase against Gram-negative bacteria.
Article Snippet: Immobilized hydrolase not only reduces the production of antibiotic-resistant bacteria, but also effectively improves the stability of hydrolase in external use.. In this study, phage hydrolase LysSSE1 against Gram-negative bacteria were surface immobilized and optimized for their bactericidal activity.. Different anti-pathogen surface materials were prepared, where LysSSE1 was immobilized on the glass surface with a silica-affinity peptide and into which different peptide linkers were introduced.

Article Title: The p.R66W Variant in RAC3 Causes Severe Fetopathy Through Variant-Specific Mechanisms
Article Snippet: To create the RAC3-R66W and constitutively active RAC3-Q61L (GTPase-deficient) variants, mutations were introduced as previously described [ ] and cloned into both pCAG-Myc and pTriEx-4 vectors (Merck, Darmstadt, Germany). .. RAC-binding regions (RBRs) of human PAK1 (aa 67–150), human MLK2 (aa 401–550), and rat N-WASP (aa 191–270) were amplified from a cDNA pool of U251MG cells or rat brain and inserted into the pGS21a vector (GenScript, Piscataway, NJ, USA) [ ]. ..

Article Title: The p.R66W Variant in RAC3 Causes Severe Fetopathy Through Variant-Specific Mechanisms.
Article Snippet: To create the RAC3-R66W and constitutively active RAC3-Q61L (GTPase-deficient) variants, mutations were introduced as previously described [22] and cloned into both pCAG-Myc and pTriEx4 vectors (Merck, Darmstadt, Germany). .. RAC-binding regions (RBRs) of human PAK1 (aa 67–150), human MLK2 (aa 401–550), and rat N-WASP (aa 191–270) were amplified from a cDNA pool of U251MG cells or rat brain and inserted into the pGS21a vector (GenScript, Piscataway, NJ, USA) [21]. ..

Article Title: Pathophysiological significance of the p.E31G variant in RAC1 responsible for a neurodevelopmental disorder with microcephaly.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Plasmid Preparation:

Article Title: Surface immobilization and properties optimization of phage hydrolase against Gram-negative bacteria.
Article Snippet: Immobilized hydrolase not only reduces the production of antibiotic-resistant bacteria, but also effectively improves the stability of hydrolase in external use.. In this study, phage hydrolase LysSSE1 against Gram-negative bacteria were surface immobilized and optimized for their bactericidal activity.. Different anti-pathogen surface materials were prepared, where LysSSE1 was immobilized on the glass surface with a silica-affinity peptide and into which different peptide linkers were introduced.

Knock-Out:

Article Title: IRF2BP2 binds to a conserved RxSVI motif of protein partners and regulates megakaryocytic differentiation.
Article Snippet: .. Generation of knockout cell lines Generation of knockout cell lines using the CRISPR/Cas9 technology was carried out with the pLenti-Cas9 vector and pGS vector (Genscript). .. Small guide RNA sequences for the targeted inactivation of Irf2bp2 and Zbtb16 in HEL cells were obtained from the Genscript library, which have been proven to be effective.

Article Title: IRF2BP2 binds to a conserved RxSVI motif of protein partners and regulates megakaryocytic differentiation
Article Snippet: .. Generation of knockout cell lines using the CRISPR/Cas9 technology was carried out with the pLenti-Cas9 vector and pGS vector (Genscript). .. Small guide RNA sequences for the targeted inactivation of Irf2bp2 and Zbtb16 in HEL cells were obtained from the Genscript library, which have been proven to be effective.

CRISPR:

Article Title: IRF2BP2 binds to a conserved RxSVI motif of protein partners and regulates megakaryocytic differentiation.
Article Snippet: .. Generation of knockout cell lines Generation of knockout cell lines using the CRISPR/Cas9 technology was carried out with the pLenti-Cas9 vector and pGS vector (Genscript). .. Small guide RNA sequences for the targeted inactivation of Irf2bp2 and Zbtb16 in HEL cells were obtained from the Genscript library, which have been proven to be effective.

Article Title: IRF2BP2 binds to a conserved RxSVI motif of protein partners and regulates megakaryocytic differentiation
Article Snippet: .. Generation of knockout cell lines using the CRISPR/Cas9 technology was carried out with the pLenti-Cas9 vector and pGS vector (Genscript). .. Small guide RNA sequences for the targeted inactivation of Irf2bp2 and Zbtb16 in HEL cells were obtained from the Genscript library, which have been proven to be effective.

Clone Assay:

Article Title: Pathophysiological significance of the p.E31G variant in RAC1 responsible for a neurodevelopmental disorder with microcephaly.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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GenScript corporation expression vector pgs21a
Expression Vector Pgs21a, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pgs21a/pgs+21a/pmc03225368-39-6-9
Average 90 stars, based on 1 article reviews
expression vector pgs21a - by Bioz Stars, 2026-09
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GenScript corporation bacterial expression vector pgs21a
A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
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GenScript corporation the bacterial expression vector pgs21a
A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of <t>pGS21a-LOC387715(ORF)m,</t> showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.
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https://www.bioz.com/product/expression+vector+pgs21a/pgs+21a/pmc02780029-97-17-29
Average 90 stars, based on 1 article reviews
the bacterial expression vector pgs21a - by Bioz Stars, 2026-09
90/100 stars
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A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

Journal:

Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

doi: 10.1016/j.pep.2007.03.017

Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

Journal:

Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

doi: 10.1016/j.pep.2007.03.017

Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

Article Snippet: LOC387715 cDNA synthesis, cloning and sequencing Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

Techniques: Expressing, Recombinant, Plasmid Preparation

A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

Journal:

Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

doi: 10.1016/j.pep.2007.03.017

Figure Lengend Snippet: A. Schematic diagram of the strategy and process for high and authentical expression of rLOC387715 in E. coli. B. EcoR I + Xho I digestion of pGS21a-LOC387715(ORF)m, showing the expected size of insert (336 bp). C. SDS-PAGE, showing the high expression of the recombinant protein in bacterium BL21(DE3) strain after induction with IPTG (1mM at 37°C for 3 h). The recombinant protein rLOC387715 was about 15% of the bacterial total proteins. Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a. Std – protein standards.

Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

Techniques: Expressing, SDS Page, Recombinant, Plasmid Preparation

A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

Journal:

Article Title: Expression of Recombinant Protein Encoded by LOC387715 in Escherichia coli

doi: 10.1016/j.pep.2007.03.017

Figure Lengend Snippet: A. Detection by anti-GST antibody. B. Detection by anti-6xHis antibody. High expression of the recombinant protein was obtained in bacterium BL21(DE3) strain by the induction of IPTG (1 mM at 37°C for 3 h). Top arrow, the recombinant protein – His-tagged GST-LOC387715 fusion protein that was expressed from pGS21a-LOC387715(ORF)m; bottom arrow, His-tagged GST that was expressed from the vector pGS21a.

Article Snippet: Self-designed synonymously modified ORF cDNA sequence of LOC387715 – LOC387715(ORF)m was synthesized and cloned in-frame into the bacterial expression vector pGS21a between EcoR I and Xho I sites by GenScript Corporation.

Techniques: Expressing, Recombinant, Plasmid Preparation