pegfpc1 expression vector (Addgene inc)
Structured Review

Pegfpc1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pegfpc1/pEGFPC1-6XHis-FLKSRP+(Plasmid+%2323001)/pmc11149935-88-12-10
Average 92 stars, based on 4 article reviews
Images
1) Product Images from "SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA"
Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA
Journal: eLife
doi: 10.7554/eLife.74940
Figure Legend Snippet:
Techniques Used: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software
Related Articles
Plasmid Preparation:Article Title: KSRP improves pancreatic beta cell function and survival Article Snippet: .. The Article Title: Tandem RNA isolation reveals functional rearrangement of RNA-binding proteins on CDKN1B/p27 Kip1 3’UTRs in cisplatin treated cells Article Snippet: In brief, 3 μl of Lipofectamine RNAiMAX was diluted in 50 μl of Opti-Mem (Gibco, # 31,985,070) and combined with 10 pmol of respective siRNA supplied in 50 μl of Opti-Mem and incubated for 15 min at room temperature (RT). .. The mixture was added to cells, which were further grown in media supplemented with 1 μg/ml of tet (Fisher, #BP-912-100) and treated with 20 μM of CP for 15 h. Plasmid transfections were performed at 70% cell confluency with 2 μg of Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA Article Snippet: Recombinant DNA reagent , pEGFP-DHX9 (plasmid) , , https://doi.org/10.18632/oncotarget.5033 , pEGFP expression vector. .. Article Title: PCBP1 inhibits the expression of oncogenic STAT3 isoform by targeting alternative splicing of STAT3 exon 23. Article Snippet: Plasmid expressing GFP tagged RBM3 was a gift from Dr. Maria Alvarado-Kristensson (Addgene plasmid #87860) [36]. .. Bacteria:Article Title: KSRP improves pancreatic beta cell function and survival Article Snippet: .. The Transfection:Article Title: Tandem RNA isolation reveals functional rearrangement of RNA-binding proteins on CDKN1B/p27 Kip1 3’UTRs in cisplatin treated cells Article Snippet: In brief, 3 μl of Lipofectamine RNAiMAX was diluted in 50 μl of Opti-Mem (Gibco, # 31,985,070) and combined with 10 pmol of respective siRNA supplied in 50 μl of Opti-Mem and incubated for 15 min at room temperature (RT). .. The mixture was added to cells, which were further grown in media supplemented with 1 μg/ml of tet (Fisher, #BP-912-100) and treated with 20 μM of CP for 15 h. Plasmid transfections were performed at 70% cell confluency with 2 μg of Recombinant:Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA Article Snippet: Recombinant DNA reagent , pEGFP-DHX9 (plasmid) , , https://doi.org/10.18632/oncotarget.5033 , pEGFP expression vector. .. Expressing:Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA Article Snippet: Recombinant DNA reagent , pEGFP-DHX9 (plasmid) , , https://doi.org/10.18632/oncotarget.5033 , pEGFP expression vector. .. Article Title: PCBP1 inhibits the expression of oncogenic STAT3 isoform by targeting alternative splicing of STAT3 exon 23. Article Snippet: Plasmid expressing GFP tagged RBM3 was a gift from Dr. Maria Alvarado-Kristensson (Addgene plasmid #87860) [36]. .. |
