Review



pcmv6 entry plasmid  (OriGene)


Bioz Verified Symbol OriGene is a verified supplier
Bioz Manufacturer Symbol OriGene manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    OriGene pcmv6 entry plasmid
    Pcmv6 Entry Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/pCMV6-Entry+Mammalian+Expression+Vector/pmc13217847-87-0-3
    Average 96 stars, based on 1687 article reviews
    pcmv6 entry plasmid - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Overexpression of FOXO1 may suppress proliferation and migration and correlate with tumor immune cells in nasopharyngeal carcinoma
    Article Snippet: NP69 cells were cultured in Keratinocyte-SFM medium supplemented with recombinant epidermal growth factor and bovine pituitary extract (Gibco, 17005042). .. PCMV6-entry-FOXO1 plasmid (RC200477) and empty vector plasmid ( PS100001 ) were purchased from OriGene Technologies in Wuxi. .. The Cell Counting Kit-8 (CCK-8) assay (Dojindo, CK04) and a colony formation assay were used to evaluate the impact of FOXO1 on cell proliferation and colony formation, respectively.

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling
    Article Snippet: Mouse Nrp1 Plasmid , Origene , Cat# MR211197. .. pCMV6-Entry Plasmid , Origene , Cat# PS100001. .. Mouse Aldh1a3 CRISPR Plasmid , Santa Cruz Biotechnology , Cat# sc-425322.


    Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation
    Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of full-length human wild-type TDP-43 (hTDP-43 , NM_007375 ) and Grx1( NM_002064.3 ) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and pCMV6-AC-Myc-DDK vector ( PS100001 , Origene Technologies, Rockville, Maryland, USA), respectively. .. Grx1-specific siRNA (D-001810-10-05) and negative control siRNAs (L-063838-01-0005) were purchased from Dharmacon.

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Cell Culture:

    Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor
    Article Snippet: .. Human CLDN3/pCMV6-Entry, human CLDN4/pCMV6-Entry, human CLDN6/pCMV-Entry, human CLDN9/pCMV6-Entry, or pCMV6-Entry purchased from OriGene Technologies, Inc. was transiently transferred into 293T cells (Thermo Fisher Scientific, HCL4517) by using Lipofectamine 2000 (Thermo Fisher Scientific), and the cells were cultured under conditions of 37° C. and 5% CO2 overnight, and then a cell suspension was prepared. .. The transfected 293T cell suspension was centrifuged to remove the supernatant, and a mouse anti-CLDN6 antibody (clone number: B1 or C7) or a mouse IgG1 control antibody (R&D Systems, Inc.) was then added and suspended to a final concentration of 30 μg/mL, 10 μg/mL, 3.3 μg/mL, or 1.1 μg/mL, and the resultant was left to stand at 4° C. for 1 hour.

    Suspension:

    Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor
    Article Snippet: .. Human CLDN3/pCMV6-Entry, human CLDN4/pCMV6-Entry, human CLDN6/pCMV-Entry, human CLDN9/pCMV6-Entry, or pCMV6-Entry purchased from OriGene Technologies, Inc. was transiently transferred into 293T cells (Thermo Fisher Scientific, HCL4517) by using Lipofectamine 2000 (Thermo Fisher Scientific), and the cells were cultured under conditions of 37° C. and 5% CO2 overnight, and then a cell suspension was prepared. .. The transfected 293T cell suspension was centrifuged to remove the supernatant, and a mouse anti-CLDN6 antibody (clone number: B1 or C7) or a mouse IgG1 control antibody (R&D Systems, Inc.) was then added and suspended to a final concentration of 30 μg/mL, 10 μg/mL, 3.3 μg/mL, or 1.1 μg/mL, and the resultant was left to stand at 4° C. for 1 hour.

    Immunocytochemistry:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Flow Cytometry:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Western Blot:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Expressing:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Construct:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Recombinant:


    Software:


    Clone Assay:

    Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation
    Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of full-length human wild-type TDP-43 (hTDP-43 , NM_007375 ) and Grx1( NM_002064.3 ) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and pCMV6-AC-Myc-DDK vector ( PS100001 , Origene Technologies, Rockville, Maryland, USA), respectively. .. Grx1-specific siRNA (D-001810-10-05) and negative control siRNAs (L-063838-01-0005) were purchased from Dharmacon.

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Over Expression:

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF clone (MG222097, MR222673, MR211197, and PS100001, Origene). .. Subsequently, 0.5 mg/mL of geneticin (10131027, Thermo Fisher Scientific) was supplemented to the culture medium for selection.

    Transfection:

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF clone (MG222097, MR222673, MR211197, and PS100001, Origene). .. Subsequently, 0.5 mg/mL of geneticin (10131027, Thermo Fisher Scientific) was supplemented to the culture medium for selection.

    Control:

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF clone (MG222097, MR222673, MR211197, and PS100001, Origene). .. Subsequently, 0.5 mg/mL of geneticin (10131027, Thermo Fisher Scientific) was supplemented to the culture medium for selection.

    Amplification:

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Cloning:

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..



    Similar Products

    94
    Sino Biological tbr1 pcmv3
    Tbr1 Pcmv3, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/Human+TBR1+Gene+ORF+cDNA+clone+expression+plasmid/10__1038_slash_s41420___026___03385___4-197-2-6
    Average 94 stars, based on 1 article reviews
    tbr1 pcmv3 - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93
    Addgene inc expression vector pcs2 8
    Expression Vector Pcs2 8, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/pCS2%2B8+(Plasmid+%2334931)/pm42887803-64-1-4
    Average 93 stars, based on 1 article reviews
    expression vector pcs2 8 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    95
    Addgene inc phlsec expression vector

    Phlsec Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/pHL-sec+(Plasmid+%2399845)/pmc13651826-223-17-20
    Average 95 stars, based on 1 article reviews
    phlsec expression vector - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    96
    Addgene inc a lentiviral expression

    A Lentiviral Expression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/LentiV_Cas9_puro+(Plasmid+%23108100)/pm42869907-516-15-21
    Average 96 stars, based on 1 article reviews
    a lentiviral expression - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    93
    Addgene inc puromycin resistance expression plasmid px459v2 0 espcas9 1 1

    Puromycin Resistance Expression Plasmid Px459v2 0 Espcas9 1 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/pX459V2%2E0-eSpCas9(1%2E1)+(Plasmid+%23108292)/pm42869113-79-13-18
    Average 93 stars, based on 1 article reviews
    puromycin resistance expression plasmid px459v2 0 espcas9 1 1 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pezy3 constitutive expression vector

    Pezy3 Constitutive Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/pEZY3+(Plasmid+%2318672)/pm42864353-463-11-15
    Average 93 stars, based on 1 article reviews
    pezy3 constitutive expression vector - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc ha tagged human c myc expression plasmid

    Ha Tagged Human C Myc Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/pCDNA3-HA-HA-humanCMYC+(Plasmid+%2374164)/pm42864323-399-18-25
    Average 93 stars, based on 1 article reviews
    ha tagged human c myc expression plasmid - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    96
    Addgene inc pcw57 1 expression vector

    Pcw57 1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/pCW57%2E1+(Plasmid+%2341393)/pmc13651888-313-1-5
    Average 96 stars, based on 1 article reviews
    pcw57 1 expression vector - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    Kyfora Bio s protein expression plasmid

    S Protein Expression Plasmid, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/PEI+MAX+Transfection+Reagent/10__1038_slash_s41541___026___01598___3-288-24-30
    Average 99 stars, based on 1 article reviews
    s protein expression plasmid - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    98
    Addgene inc vsv g envelope expressing plasmid pmd2 g

    Vsv G Envelope Expressing Plasmid Pmd2 G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+plasmids/pMD2%2EG+(Plasmid+%2312259)/10__1038_slash_s41467___026___78252___y-394-25-30
    Average 98 stars, based on 1 article reviews
    vsv g envelope expressing plasmid pmd2 g - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    Image Search Results


    Journal: iScience

    Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease

    doi: 10.1016/j.isci.2026.117642

    Figure Lengend Snippet:

    Article Snippet: The cDNAs encoding the extracellular domains of the HLA-DR3 α- and β-chains were separately cloned into the pHLsec expression vector (Addgene plasmid #99845).

    Techniques: Isolation, Cell Isolation, DNA Extraction, Quantitation Assay, Plasmid Preparation, Extraction, Recombinant, Expressing, Software