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expression cassette  (Addgene inc)


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    Structured Review

    Addgene inc expression cassette
    Expression Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+cassettes/pcDXC3GMS+(Plasmid+%2349031)/pm41922314-237-5-9
    Average 91 stars, based on 5 article reviews
    expression cassette - by Bioz Stars, 2026-11
    91/100 stars

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    Related Articles

    Expressing:

    Article Title: Stereoselectivity and functional plasticity of a common ligand-binding pocket in TRPM3
    Article Snippet: The coding sequence of murine TRPM3 (NCBI Reference Sequence: NP_001030319.1), truncated at amino acid residue 1344, was cloned into a modified pcDNA5/FRT/TR expression vector (Invitrogen/Thermo Scientific). .. To do so, the entire expression cassette of pcDXC3GMS (Addgene #49031) containing a C-terminal SBP-tag was excised with HindIII and ApaI and ligated into HindIII/ApaI-digested pcDNA5/FRT/TR to generate p5TO-CSBP. ..

    Article Title: Stereoselectivity and functional plasticity of a common ligand-binding pocket in TRPM3.
    Article Snippet: The coding sequence of murine TRPM3 (NCBI Reference Sequence: NP_001030319.1), truncated at amino acid residue 1344, was cloned into a modified pcDNA5/FRT/TR expression vector (Invitrogen/Thermo Scientific). .. To do so, the entire expression cassette of pcDXC3GMS (Addgene #49031) containing a Cterminal SBP-tag was excised with HindIII and ApaI and ligated into HindIII/ApaI-digested pcDNA5/FRT/TR to generate p5TO-CSBP. ..

    Article Title: Structural basis for ion selectivity in TMEM175 K+ channels
    Article Snippet: .. Expression and purification of MtTMEM175/fluorescent Nb51H01 from HEK293 cells Low passage HEK293 cells were transiently transfected with MtTMEM175 (cloned into pcDXC3MS [Brunner et al., 2014; Schenck et al., 2017], Addgene #49030) or Nb51H01 (cloned into pcDXC3GMS [Brunner et al., 2014; Schenck et al., 2017], Addgene #49031) using Fugene following the manufacturers protocol. ..

    Article Title: MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
    Article Snippet: BtTMEM206 was cloned from the IMAGE clone IRCJp5010B0531D (Source BioSciences) and mTMEM16F was synthesized (Genscript) and cloned into a modified pcDNA5/FRT/TR expression vector (Invitrogen/Thermo Scientific). .. For this, the entire expression cassette of pcDXC3GMS (Addgene #49031) with a C-terminal Venus YFP (flanked by the KpnI sites) and a streptavidin-binding protein tag (SBP-tag) was excised with HindIII and ApaI and ligated into HindIII/ApaI-digested pcDNA5/FRT/TR to generate p5TO-CV. ..

    Clone Assay:

    Article Title: Structural basis for ion selectivity in TMEM175 K+ channels
    Article Snippet: For cell surface labeling of MtTMEM175 with Nb51H01 and for size exclusion profiles of MtTMEM175 expressed in HEK cells, MtTMEM175 was cloned into pcDXC3MS (Brunner et al., 2014; Schenck et al., 2017) (Addgene #49030) to include a streptavidin binding peptide tag for purification (SBP) (Keefe et al., 2001). .. Nb51H01 was cloned into pcDXC3GMS (Addgene #49031) to include a vYFP tag in addition to the purification tag. ..

    Purification:

    Article Title: Structural basis for ion selectivity in TMEM175 K+ channels
    Article Snippet: For cell surface labeling of MtTMEM175 with Nb51H01 and for size exclusion profiles of MtTMEM175 expressed in HEK cells, MtTMEM175 was cloned into pcDXC3MS (Brunner et al., 2014; Schenck et al., 2017) (Addgene #49030) to include a streptavidin binding peptide tag for purification (SBP) (Keefe et al., 2001). .. Nb51H01 was cloned into pcDXC3GMS (Addgene #49031) to include a vYFP tag in addition to the purification tag. ..

    Article Title: Structural basis for ion selectivity in TMEM175 K+ channels
    Article Snippet: .. Expression and purification of MtTMEM175/fluorescent Nb51H01 from HEK293 cells Low passage HEK293 cells were transiently transfected with MtTMEM175 (cloned into pcDXC3MS [Brunner et al., 2014; Schenck et al., 2017], Addgene #49030) or Nb51H01 (cloned into pcDXC3GMS [Brunner et al., 2014; Schenck et al., 2017], Addgene #49031) using Fugene following the manufacturers protocol. ..

    Transfection:

    Article Title: Structural basis for ion selectivity in TMEM175 K+ channels
    Article Snippet: .. Expression and purification of MtTMEM175/fluorescent Nb51H01 from HEK293 cells Low passage HEK293 cells were transiently transfected with MtTMEM175 (cloned into pcDXC3MS [Brunner et al., 2014; Schenck et al., 2017], Addgene #49030) or Nb51H01 (cloned into pcDXC3GMS [Brunner et al., 2014; Schenck et al., 2017], Addgene #49031) using Fugene following the manufacturers protocol. ..



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