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expresshyb solution containing 1x10 6 cpm/ml 32 p-labeled thymidine kinase probe  (Becton Dickinson)

 
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    Becton Dickinson expresshyb solution containing 1x10 6 cpm/ml 32 p-labeled thymidine kinase probe
    Expresshyb Solution Containing 1x10 6 Cpm/Ml 32 P Labeled Thymidine Kinase Probe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expresshybtm+buffer/pdgfr%CE%B1+antibody/pmc01829174-75-30-15
    Average 90 stars, based on 1 article reviews
    expresshyb solution containing 1x10 6 cpm/ml 32 p-labeled thymidine kinase probe - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Filtration:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Staining:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Cell Culture:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    In Vitro:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Isolation:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Co-Culture Assay:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Expressing:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Software:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Cell Based Assay:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Concentration Assay:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Injection:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    In Vivo:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Saline:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Western Blot:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Plasmid Purification:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Immunofluorescence:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    MANN-WHITNEY:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Electron Microscopy:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Labeling:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Incubation:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Virus:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Recombinant:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Modification:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    In Situ:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Avidin-Biotin Assay:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Plasmid Preparation:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    SYBR Green Assay:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).

    Cell Counting:

    Article Title: Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model
    Article Snippet: Differentiated cardiovascular cells and cardiac tissue sheets were dissociated by incubation with Accutase and stained with one or a combination of the following surface markers: anti-PDGFRβ conjugated with phycoerythrin (PE), clone 28d4, 1:100 (BD, Franklin Lakes, NJ, USA) for MCs, and anti-VE-cadherin conjugated with phycoerythrin (PE), clone 55-7h1, 1:100 (BD) for ECs.

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Article Snippet: The following primary antibodies were used for immunoblotting: anti- Flag (1:5000; SigmaAldrich, F7425), anti- Myc (1:5000; Proteintech, 16286- 1- AP), anti- hemagglutinin (1:5000; Sigma- Aldrich, H3663), anti- RNF220 (1:1000; Sigma- Aldrich, HPA027578), anti- MBP (1:1000; SigmaAldrich, ab9348), anti- PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190- 1- Ig), anti- PLP (1:1000; Cell Signaling Technology, 85971S), anti- MOG (1:1000; Proteintech, 12690- 1- AP), anti- MAG (1:1000; Proteintech, 14386- 1- AP), anti- Olig1 (1:1000; Santa Cruz Biotechnology, SC- 166257), anti- Olig2 (1:1000; Millipore, MABN50), antiubiquitin (1:1000; Santa Cruz Biotechnology, SC- 8017), and anti–α- tubulin (1:5000; Proteintech, 66031- 1- Ig).

    Article Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-Flag (1:5000; Sigma-Aldrich, F7425), anti-Myc (1:5000; Proteintech, 16286-1-AP), anti-hemagglutinin (1:5000; Sigma-Aldrich, H3663), anti-RNF220 (1:1000; Sigma-Aldrich, HPA027578), anti-MBP (1:1000; Sigma-Aldrich, ab9348), anti-PDGFRα (1:1000; BD Biosciences, 558774), anti–glial fibrillary acidic protein (GFAP) (1:1000; Proteintech, 60190-1-Ig), anti-PLP (1:1000; Cell Signaling Technology, 85971S), anti-MOG (1:1000; Proteintech, 12690-1-AP), anti-MAG (1:1000; Proteintech, 14386-1-AP), anti-Olig1 (1:1000; Santa Cruz Biotechnology, SC-166257), anti-Olig2 (1:1000; Millipore, MABN50), anti-ubiquitin (1:1000; Santa Cruz Biotechnology, SC-8017), and anti–α-tubulin (1:5000; Proteintech, 66031-1-Ig).

    Article Title: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
    Article Snippet: MCs were stained with an anti-PDGFRβ antibody (1:100, #558821, BD Bioscience) labelled with Grn-PE and an anti-Thy1 antibody (BioLegend, San Diego, USA, 1:100) labelled with APC in EDTA at room temperature for 30 min. CMs were first fixed in 4% paraformaldehyde (PFA) for 15 min and washed twice with saponin-EDTA (0.25%, Sigma-Aldrich, St. Louis, USA), following by staining with an anti-cardiac isoform of Troponin T (cTnT) antibody (1:50, #MS-295-P, mouse monoclonal, clone 13–11, Thermo Fisher Scientific) labeled with Alexa-488 using the Zenon technology (#Z25002, Thermo Fisher Scientific) according to the manufacturer’s instructions, diluted in saponin-EDTA for 30 min at room temperature.

    Article Title: Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.
    Article Snippet: Mural cells were identified using anti-PDGFRβ conjugated with phycoerythrin (PE) (clone 28d4, 1:100, BD, Franklin Lakes, NJ, USA), while endothelial cells were marked with anti-VE-cadherin conjugated with allophycocyanin (APC) (clone 55-7h1, 1:100, BD).

    Article Title: Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells
    Article Snippet: Mouse monoclonal to PDGFRβ (clone 28D4), BV786 , BD Biosciences , Cat. #743038; RRID: AB_2741234.

    Article Title: Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling
    Article Snippet: After filtration using a 70-μm cell strainer to remove debris and bone fragments, cells were stained with APC-conjugated mouse PDGFRα (clone APA5), FITC-conjugated mouse Sca-1 (clone D7), PE-cy7-conjugated mouse CD45 (clone 30-F11), mouse TER119 (clone TER-119), and mouse CD31 (clone 390) antibodies (BD Biosciences, Franklin Lakes, NJ, USA).



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