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Protein Sciences Inc expressf+ serum-free high-density insect cells
Expressf+ Serum Free High Density Insect Cells, supplied by Protein Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expressf++cells/expressf++insect+cells/pm10504713-11-22-24
Average 90 stars, based on 1 article reviews
expressf+ serum-free high-density insect cells - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Structural features of the Bluetongue virus NS2 protein.
Article Snippet: Bluetongue virus (BTV) non-structural protein 2 (NS2) belongs to a class of highly conserved proteins found in members of the orbivirus genus of the reoviridae.. NS2 forms large multimeric complexes, localizes to cytoplasmic inclusion bodies in the infected cells and binds non-sequence specifically single-stranded RNA (ssRNA).. Due to its ability to bind ssRNA, it has been suggested that the protein is involved in the selection and condensation of the BTV ssRNA segments prior to genome encapsidation.

Article Title: Identification of small molecule inhibitors of p27 Kip1 ubiquitination by high‐throughput screening
Article Snippet: The recombinant plasmids were then transformed into DH10Bac cells followed by transfection of the obtained recombinant bacmids into ExpressSF+ Serum‐Free Insect Cells (Protein Sciences) using Cellfectin II (Invitrogen, Carlsbad, CA, USA).

Article Title: New products
Article Snippet: The system uses a repressor to regulate transcription, eliminating the nonspecific effects of transactivators used by other tetracycline-regulated systems. http://www.invitrogen.com RIN: 1202 High-density cells Protein Sciences’ (Meriden, CT) proprietary expresSF+ serum-free high-density insect cells for use in the baculovirus expression vector system are suitable for research and cGMP production of recombinant proteins.

Article Title: Structural and Biophysical Characterization of the Proteins Interacting with the Herpes Simplex Virus 1 Origin of Replication
Article Snippet: expresSF+ insect cells (Protein Sciences Corp.) were maintained in Sf900II medium supplemented with 100 units ml −1 penicillin/streptomycin (Invitrogen) and grown in 2.5-liter suspension flasks (Fernbach) to a cell density of 4 × 10 6 cells ml −1 .

Article Title: Structural basis for the specific recognition of the major antigenic peptide from the Japanese cedar pollen allergen Cry j 1 by HLA-DP5.
Article Snippet: Spodoptera frugiperda cells (5 × 105 cells/ml), derived from expresSF+® Insect Cells (Protein Sciences), were cultured in Sf-900TM III SFM (Life Technologies) and infected with the viral stock at a multiplicity of infection of 0.2.

Article Title: Osteoclast-secreted CTHRC1 in the coupling of bone resorption to formation
Article Snippet: In brief, mouse Cthrc1 cDNA containing a His-tag in a pPSC8 vector (Protein Science) was cotransfected with AcNPV DNA (Invitrogen) using CELLFECTIN (Invitrogen) into Sf9 cells, which were then infected into expresSF + insect cells (Protein Sciences) using recombinant viruses.

Article Title: Structural and Biophysical Characterization of the Proteins Interacting with the Herpes Simplex Virus 1 Origin of Replication
Article Snippet: Insect Cell Expression and Purification of ICP8 C—expresSF insect cells (Protein Sciences Corp.) were maintained in Sf900II medium supplemented with 100 units ml 1 penicillin/streptomycin (Invitrogen) and grown in 2.5-liter suspension flasks (Fernbach) to a cell density of 4 106 cells ml 1.

Infection:

Article Title: Crystal Structure of West Nile Virus Envelope Glycoprotein Reveals Viral Surface Epitopes
Article Snippet: Nucleotides 925 to 2142 containing the E gene of the polyprotein of WNV strain 2741 (GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AF206518","term_id":"7717200","term_text":"AF206518"}} AF206518 ) ( 2 ) were inserted into the pPSC12 baculovirus cloning vector (Protein Sciences Corporation, Meriden, CT). .. ExpresSF+ cells from Spodoptera frugiperda (Protein Sciences Corporation) were infected with the recombinant baculovirus, causing them to secrete soluble E protein (sE) into the medium. .. The medium was concentrated on a Biomax 30 Pellicon XL concentrator (Millipore), adjusted to pH 7.1 with 1 N NaOH, and loaded on a preequilibrated immunoaffinity column.

Recombinant:

Article Title: Crystal Structure of West Nile Virus Envelope Glycoprotein Reveals Viral Surface Epitopes
Article Snippet: Nucleotides 925 to 2142 containing the E gene of the polyprotein of WNV strain 2741 (GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AF206518","term_id":"7717200","term_text":"AF206518"}} AF206518 ) ( 2 ) were inserted into the pPSC12 baculovirus cloning vector (Protein Sciences Corporation, Meriden, CT). .. ExpresSF+ cells from Spodoptera frugiperda (Protein Sciences Corporation) were infected with the recombinant baculovirus, causing them to secrete soluble E protein (sE) into the medium. .. The medium was concentrated on a Biomax 30 Pellicon XL concentrator (Millipore), adjusted to pH 7.1 with 1 N NaOH, and loaded on a preequilibrated immunoaffinity column.



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List of commercially approved human and veterinary vaccines developed using an insect cell expression system.

Journal: Vaccines

Article Title: Challenges and Opportunities in the Process Development of Chimeric Vaccines

doi: 10.3390/vaccines11121828

Figure Lengend Snippet: List of commercially approved human and veterinary vaccines developed using an insect cell expression system.

Article Snippet: Influenza Virus , FlublokQuadrivalent ® , HA quadrivalent/Subunit , expresSF+ cells , Sanofi Pasteur, Paris, France , [ ] .

Techniques: Vaccines, Expressing, Virus

Summary of vaccine produced in BEVS and BacMam platform in the campaign against influenza-virus infection

Journal: Applied Microbiology and Biotechnology

Article Title: Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies

doi: 10.1007/s00253-021-11615-1

Figure Lengend Snippet: Summary of vaccine produced in BEVS and BacMam platform in the campaign against influenza-virus infection

Article Snippet: , expresSF + cells , HA from A/H1N1, A/H3N2, and two strain B , ND , Approved (FlubloK, Sanofi Pasteur-Protein Science Corporation) , Buckland et al. ( ) .

Techniques: Produced, Plasmid Preparation, Expressing, Adjuvant