maximal treadmill exercise test  (Columbus Instruments)

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: High‐Calorie Diet During Pregnancy Leads to Muscular Fibrosis and Neuromuscular Damage in Offspring Mice

doi: 10.1002/jcsm.70027

Figure Lengend Snippet: Maternal high‐calorie diet markedly alters muscle fitness function in PolgA offspring mice. (A and B) Body weight (A) and calorie intake (B) in PolgA offspring with/without M‐HFD challenge during pregnancy ( n = 6/group). (C) Relative inguinal white adipose tissue (ingWAT) and epididymal (epi)WAT weights in PolgA offspring (body weight was used for normalization; n = 6/group). (D) Time‐resolved oxygen consumption (left) and carbon dioxide production (right) of 6‐month‐old PolgA offspring born from mothers fed HFD during pregnancy ( n = 6/group). (E) Relative maximal grip strength (body weight was used for normalization) (left) and 10‐repitition endurance grip strength (middle) and area under the curve (AUC) of endurance strength (right) in 6‐month‐old PolgA offspring with/without M‐HFD challenge ( n = 6/group). (F) Total exercise time (left) and distance (right) through cardiorespiratory exercise test in 6‐month‐old PolgA offspring in response to M‐HFD ( n = 6/group). Mean ± SEM, and each dot represents one litter. * p < 0.05, ** p < 0.01 and *** p < 0.001 in WT vs. PolgA mut , and # p < 0.05, ## p < 0.01 and ### p < 0.001 in CD vs. HFD by two‐sided p values by two‐way ANOVA followed by Tukey's test (A–F).

Article Snippet: Endurance treadmill exercise capacity test was determined using a 25° inclined treadmill exercise along with the indirect respiratory metabolic measurements using a treadmill respiratory measurement system (Oxymax fast 4 lane modular treadmill system; Columbus Instruments) according to the manufacturer's protocols and guidelines, as described in our previous studies [ , ].

Techniques:

Journal: Journal of Physiology and Biochemistry

Article Title: Polarized or threshold training: is there a superior training intensity distribution to improve V̇O 2 max, endurance capacity and mitochondrial function? A study in Wistar Rat models

doi: 10.1007/s13105-025-01079-6

Figure Lengend Snippet: Exercise testing: A relative to body weight maximal oxygen consumption (V̇O 2 max; mL . Kg −1. min −1 ) at baseline, intermediate (four weeks) and final assessment (eight weeks); B absolute V̇O 2 max (mL.min −1 ) at baseline, intermediate and final assessment; C total distance performed (in meters; m) during the endurance capacity test at baseline, intermediate and final assessment. Data are presented as mean ± standard deviation. CON: Control group: POL: Polarized training group; THR: Threshold training group. Asterisks highlight the existence of statistically significant differences for CON in comparison to POL and THR (* p < 0.05)

Article Snippet: V̇O 2 max was determined through a maximal treadmill exercise test (Metabolic Modular Treadmill; Columbus Instruments, Columbus, USA) with respiratory gas analysis following adequate calibration according to manufacturer instructions using primary standard grade calibration gas (Acail Gas, Portugal) and drierite columns (Calcium Sulfate with Indicator, Sigma-Aldrich; St. Louis, USA) to ensure moisture absence in the gas sample.

Techniques: Standard Deviation, Control, Comparison