Journal: Virologica Sinica

Article Title: A modular APEX biosensor enables sensitive and visualized monitoring of viral protease activity

doi: 10.1016/j.virs.2026.08.001

Figure Lengend Snippet: Dose-response and fluorescence imaging of antiviral inhibitors with C3SIR biosensor. A Dose-response curves of the C3SIR biosensor for Boceprevir, Telaprevi and Narlaprevir were marked in red. Cell viability (blue) was assessed relative to the solvent (DMSO) control group. IC50 and R-squared values of each inhibitor were estimated via nonlinear regression. B HEK293T cells were transfected with the C3SIR sensor. Culture medium containing inhibitors at concentrations of 50, 20, 10, 5 and 2 μM was added 6 h post-transfection. Fluorescence microscopy images were captured 24 h post-transfection. C Dose-response curves of the C3SIR biosensor for Oseltamivir, Ritonavir and Rupintrivir were marked in red. Cell viability (blue) was assessed relative to the solvent (DMSO) control group. IC50 and R-squared values of each inhibitor were estimated via nonlinear regression. D HEK293T cells were transfected with the C3SIR sensor. Culture medium containing inhibitors at concentrations of 50, 20, 10, 5 and 2 μM was added 6 h post-transfection. Fluorescence microscopy images were captured 24 h post-transfection. IC 50 and R 2 values were estimated via nonlinear regression curve fitting to single experimental data for each inhibitor. All images show the same magnification, scale bar: 1000 μm ( B , D ).

Article Snippet: The C3SIR (EV-71) biosensor was validated with Rupintrivir (TargetMOI).

Techniques: Fluorescence, Imaging, Solvent, Control, Transfection, Microscopy