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plvx ef1a teton ires g418  (Addgene inc)


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    Addgene inc plvx ef1a teton ires g418
    Plvx Ef1a Teton Ires G418, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eto/pLVX-EF1a-tetOn-IRES-G418+(EtO)+(Plasmid+%2384776)/pm40847005-93-33-34
    Average 93 stars, based on 8 article reviews
    plvx ef1a teton ires g418 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Methods for Mitochondrial DNA Damage and Depletion in Immortalized Trabecular Meshwork Cells
    Article Snippet: .. For all plasmids used, plasmid maps are accessible from Addgene using the identifiers provided. pLVX-EF1a-tetOn-IRES-G418 (EtO) was a gift from Fred Gage (Addgene plasmid # 84776), pMA3287 was a gift from Mikhail Alexeyev (Addgene plasmid # 46883), and psPAX2 and pMD2.G were gifts from Didier Trono (Addgene plasmid # 12260 and # 12259). ..

    Article Title: Designed NGF mimetics with reduced nociceptive signatures in neurons
    Article Snippet: Cells were passaged every 3-4 days using trypsin-EDTA (Gibco, Cat #25300054), maintained at 37°C in a humidified incubator and routinely tested for mycoplasma. .. For conversion to neurons, stable lines were generated by transducing two lentiviruses produced in HEK293FT cells with packaging plasmids psPAX2 and pMD2.G following the Addgene protocol: The first one containing pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A), a gift from Fred Gage (Addgene plasmid # 84777), a the second one a tet-operator plasmid (Addgene # 84776), followed by 4 days of puromycin (1μg/ml) and G418 (150 μg/ml) selection. ..

    Transduction:

    Article Title: Differential effects of lithium on metabolic dysfunctions in astrocytes derived from bipolar disorder patients
    Article Snippet: iNs were generated using the protocol detailed in [ ] with some modifications. hESCs (WA01; H1) were maintained on Matrigel-coated plates (Corning 354230) for 24 h prior to seeding in mTeSR-1 (STEMCELL Technologies ST85850) supplemented with bFGF (20 ng/ml, R&D systems 4114-TC-01M). .. For iN precursor cell (iN_ESC) generation, hESCs were seeded at 1 × 10 5 cells per Matrigel-coated 35 mm dish one day prior to transduction, followed by lentiviral infection with pLVX-TREtight-Ngn2:2 A:Ascl1-PGK-Puro (Addgene #84777) and pLVX-EF1a-tetOn-IRES-G418 (Addgene #84776) at MOI = 1. .. Selection was performed using puromycin (1 μg/ml, Thermo Fisher Scientific A1113803) and G418 (0.5 mg/ml, Thermo Fisher Scientific 10131035).

    Article Title: Differential effects of lithium on metabolic dysfunctions in astrocytes derived from bipolar disorder patients.
    Article Snippet: Generation of induced Neurons (iNs) from human embryonic stem cells (hESCs) iNs were generated using the protocol detailed in [35] with some modifications. hESCs (WA01; H1) were maintained on Matrigel-coated plates (Corning 354230) for 24 h prior to seeding in mTeSR-1 (STEMCELL Technologies ST85850) supplemented with bFGF (20 ng/ml, R&D systems 4114-TC-01M). .. For iN precursor cell (iN_ESC) generation, hESCs were seeded at 1 × 105 cells per Matrigel-coated 35mm dish one day prior to transduction, followed by lentiviral infection with pLVX-TREtight-Ngn2:2 A:Ascl1-PGK-Puro (Addgene #84777) and pLVX-EF1a-tetOn-IRES-G418 (Addgene #84776) at MOI= 1. .. Selection was performed using puromycin (1 μg/ml, Thermo Fisher Scientific A1113803) and G418 (0.5 mg/ml, Thermo Fisher Scientific 10131035).

    Infection:

    Article Title: Differential effects of lithium on metabolic dysfunctions in astrocytes derived from bipolar disorder patients
    Article Snippet: iNs were generated using the protocol detailed in [ ] with some modifications. hESCs (WA01; H1) were maintained on Matrigel-coated plates (Corning 354230) for 24 h prior to seeding in mTeSR-1 (STEMCELL Technologies ST85850) supplemented with bFGF (20 ng/ml, R&D systems 4114-TC-01M). .. For iN precursor cell (iN_ESC) generation, hESCs were seeded at 1 × 10 5 cells per Matrigel-coated 35 mm dish one day prior to transduction, followed by lentiviral infection with pLVX-TREtight-Ngn2:2 A:Ascl1-PGK-Puro (Addgene #84777) and pLVX-EF1a-tetOn-IRES-G418 (Addgene #84776) at MOI = 1. .. Selection was performed using puromycin (1 μg/ml, Thermo Fisher Scientific A1113803) and G418 (0.5 mg/ml, Thermo Fisher Scientific 10131035).

    Article Title: Differential effects of lithium on metabolic dysfunctions in astrocytes derived from bipolar disorder patients.
    Article Snippet: Generation of induced Neurons (iNs) from human embryonic stem cells (hESCs) iNs were generated using the protocol detailed in [35] with some modifications. hESCs (WA01; H1) were maintained on Matrigel-coated plates (Corning 354230) for 24 h prior to seeding in mTeSR-1 (STEMCELL Technologies ST85850) supplemented with bFGF (20 ng/ml, R&D systems 4114-TC-01M). .. For iN precursor cell (iN_ESC) generation, hESCs were seeded at 1 × 105 cells per Matrigel-coated 35mm dish one day prior to transduction, followed by lentiviral infection with pLVX-TREtight-Ngn2:2 A:Ascl1-PGK-Puro (Addgene #84777) and pLVX-EF1a-tetOn-IRES-G418 (Addgene #84776) at MOI= 1. .. Selection was performed using puromycin (1 μg/ml, Thermo Fisher Scientific A1113803) and G418 (0.5 mg/ml, Thermo Fisher Scientific 10131035).

    Recombinant:

    Article Title: Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patients.
    Article Snippet: Cat#711-165-152 Anti-Mouse HRP Detection Module Bio-Techne Cat#DM-002 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#C3040H Cyto-Tune Sendai reprogramming kit Invitrogen Cat#A16517 Biological samples Fibroblast cultures from cohort (see Table S1) this study and Mertens et al., 2015a Table S1 Chemicals, peptides, and recombinant proteins Trizol-LS reagent Thermo Fisher Scientific Cat#10296010 Puromycin Sigma Aldrich Cat#P8833 Collagenase IV dissociation reagent Thermo Fisher Scientific Cat#17104019 TrypLE dissociation reagent Thermo Fisher Scientific Cat#12604013 B-27 supplement Thermo Fisher Scientific Cat#17504044 N2 supplement Thermo Fisher Scientific Cat#17502048 Matrigel coating reagent Thermo Fisher Scientific Cat#3432-005-01 Non-Essential Amino Acids (NEAA) supplement Thermo Fisher Scientific Cat#M7145 Laminin coating reagent Sigma Aldrich Cat#L2020 Geltrex coating reagent Thermo Fisher Scientific Cat#A1413201 (Continued on next page) Cell Stem Cell 28, 1–16.e1–e6, September 2, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pLVXUbC-rtTA-Ngn2:2A:Ascl1 (Herdy et al., 2019) Addgene #127289 pLVXUbC-rtTA-Ngn2:2A:EGFP (Schafer et al., 2019) Addgene #84777 pLVXTP-GcAMP6m This study N/A pCSC-hSyn1::dsRed (Mertens et al., 2015b) N/A pLVX-EF1a-tetOn-IRES-G418 (EtO) (Mertens et al., 2015a) Addgene #84776 Software and algorithms STAR Aligner https://github.com/alexdobin/STAR N/A Babraham Bioinformatics TrimGalore https://www.bioinformatics.babraham.ac.uk N/A Samtools samtools.github.io N/A Hypergeometric Optimization of Motif EnRichment (HOMER) homer.ucsd.edu N/A DESeq2 https://github.com/mikelove/DESeq2 N/A deepTools https://deeptools.readthedocs.io N/A bedtools https://bedtools.readthedocs.io N/A MBF Bioscience Neurolucida https://www.mbfbioscience.com/neurolucida N/A Integrative Genomics Viewer - Broad Institute (IGV) software.broadinstitute.org/software/igv N/A GraphPad Prism graphpad.com N/A FlowJo flowjo.com N/A STRING-db string-db.org N/A DAVID Functional Annotation https://david.ncifcrf.gov N/A Reduce + Visualize Gene Ontology (REVIGO) revigo.irb.hr N/A FACSChorus Software Becton Dickinson N/A ChAMP 10.18129/B9.bioc.ChAMP N/A RnBeads rnbeads.org N/A DNAmAGE http://dnamage.genetics.ucla.edu N/A Rank Rank Hypergeometric Overlap (RRHO) 10.18129/B9.bioc.RRHO N/A ZEN Imaging Software Carl Zeiss N/A ImageJ https://imagej.nih.gov/ij N/A ..

    Software:

    Article Title: Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patients.
    Article Snippet: Cat#711-165-152 Anti-Mouse HRP Detection Module Bio-Techne Cat#DM-002 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#C3040H Cyto-Tune Sendai reprogramming kit Invitrogen Cat#A16517 Biological samples Fibroblast cultures from cohort (see Table S1) this study and Mertens et al., 2015a Table S1 Chemicals, peptides, and recombinant proteins Trizol-LS reagent Thermo Fisher Scientific Cat#10296010 Puromycin Sigma Aldrich Cat#P8833 Collagenase IV dissociation reagent Thermo Fisher Scientific Cat#17104019 TrypLE dissociation reagent Thermo Fisher Scientific Cat#12604013 B-27 supplement Thermo Fisher Scientific Cat#17504044 N2 supplement Thermo Fisher Scientific Cat#17502048 Matrigel coating reagent Thermo Fisher Scientific Cat#3432-005-01 Non-Essential Amino Acids (NEAA) supplement Thermo Fisher Scientific Cat#M7145 Laminin coating reagent Sigma Aldrich Cat#L2020 Geltrex coating reagent Thermo Fisher Scientific Cat#A1413201 (Continued on next page) Cell Stem Cell 28, 1–16.e1–e6, September 2, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pLVXUbC-rtTA-Ngn2:2A:Ascl1 (Herdy et al., 2019) Addgene #127289 pLVXUbC-rtTA-Ngn2:2A:EGFP (Schafer et al., 2019) Addgene #84777 pLVXTP-GcAMP6m This study N/A pCSC-hSyn1::dsRed (Mertens et al., 2015b) N/A pLVX-EF1a-tetOn-IRES-G418 (EtO) (Mertens et al., 2015a) Addgene #84776 Software and algorithms STAR Aligner https://github.com/alexdobin/STAR N/A Babraham Bioinformatics TrimGalore https://www.bioinformatics.babraham.ac.uk N/A Samtools samtools.github.io N/A Hypergeometric Optimization of Motif EnRichment (HOMER) homer.ucsd.edu N/A DESeq2 https://github.com/mikelove/DESeq2 N/A deepTools https://deeptools.readthedocs.io N/A bedtools https://bedtools.readthedocs.io N/A MBF Bioscience Neurolucida https://www.mbfbioscience.com/neurolucida N/A Integrative Genomics Viewer - Broad Institute (IGV) software.broadinstitute.org/software/igv N/A GraphPad Prism graphpad.com N/A FlowJo flowjo.com N/A STRING-db string-db.org N/A DAVID Functional Annotation https://david.ncifcrf.gov N/A Reduce + Visualize Gene Ontology (REVIGO) revigo.irb.hr N/A FACSChorus Software Becton Dickinson N/A ChAMP 10.18129/B9.bioc.ChAMP N/A RnBeads rnbeads.org N/A DNAmAGE http://dnamage.genetics.ucla.edu N/A Rank Rank Hypergeometric Overlap (RRHO) 10.18129/B9.bioc.RRHO N/A ZEN Imaging Software Carl Zeiss N/A ImageJ https://imagej.nih.gov/ij N/A ..

    Functional Assay:

    Article Title: Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patients.
    Article Snippet: Cat#711-165-152 Anti-Mouse HRP Detection Module Bio-Techne Cat#DM-002 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#C3040H Cyto-Tune Sendai reprogramming kit Invitrogen Cat#A16517 Biological samples Fibroblast cultures from cohort (see Table S1) this study and Mertens et al., 2015a Table S1 Chemicals, peptides, and recombinant proteins Trizol-LS reagent Thermo Fisher Scientific Cat#10296010 Puromycin Sigma Aldrich Cat#P8833 Collagenase IV dissociation reagent Thermo Fisher Scientific Cat#17104019 TrypLE dissociation reagent Thermo Fisher Scientific Cat#12604013 B-27 supplement Thermo Fisher Scientific Cat#17504044 N2 supplement Thermo Fisher Scientific Cat#17502048 Matrigel coating reagent Thermo Fisher Scientific Cat#3432-005-01 Non-Essential Amino Acids (NEAA) supplement Thermo Fisher Scientific Cat#M7145 Laminin coating reagent Sigma Aldrich Cat#L2020 Geltrex coating reagent Thermo Fisher Scientific Cat#A1413201 (Continued on next page) Cell Stem Cell 28, 1–16.e1–e6, September 2, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pLVXUbC-rtTA-Ngn2:2A:Ascl1 (Herdy et al., 2019) Addgene #127289 pLVXUbC-rtTA-Ngn2:2A:EGFP (Schafer et al., 2019) Addgene #84777 pLVXTP-GcAMP6m This study N/A pCSC-hSyn1::dsRed (Mertens et al., 2015b) N/A pLVX-EF1a-tetOn-IRES-G418 (EtO) (Mertens et al., 2015a) Addgene #84776 Software and algorithms STAR Aligner https://github.com/alexdobin/STAR N/A Babraham Bioinformatics TrimGalore https://www.bioinformatics.babraham.ac.uk N/A Samtools samtools.github.io N/A Hypergeometric Optimization of Motif EnRichment (HOMER) homer.ucsd.edu N/A DESeq2 https://github.com/mikelove/DESeq2 N/A deepTools https://deeptools.readthedocs.io N/A bedtools https://bedtools.readthedocs.io N/A MBF Bioscience Neurolucida https://www.mbfbioscience.com/neurolucida N/A Integrative Genomics Viewer - Broad Institute (IGV) software.broadinstitute.org/software/igv N/A GraphPad Prism graphpad.com N/A FlowJo flowjo.com N/A STRING-db string-db.org N/A DAVID Functional Annotation https://david.ncifcrf.gov N/A Reduce + Visualize Gene Ontology (REVIGO) revigo.irb.hr N/A FACSChorus Software Becton Dickinson N/A ChAMP 10.18129/B9.bioc.ChAMP N/A RnBeads rnbeads.org N/A DNAmAGE http://dnamage.genetics.ucla.edu N/A Rank Rank Hypergeometric Overlap (RRHO) 10.18129/B9.bioc.RRHO N/A ZEN Imaging Software Carl Zeiss N/A ImageJ https://imagej.nih.gov/ij N/A ..

    Imaging:

    Article Title: Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patients.
    Article Snippet: Cat#711-165-152 Anti-Mouse HRP Detection Module Bio-Techne Cat#DM-002 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#C3040H Cyto-Tune Sendai reprogramming kit Invitrogen Cat#A16517 Biological samples Fibroblast cultures from cohort (see Table S1) this study and Mertens et al., 2015a Table S1 Chemicals, peptides, and recombinant proteins Trizol-LS reagent Thermo Fisher Scientific Cat#10296010 Puromycin Sigma Aldrich Cat#P8833 Collagenase IV dissociation reagent Thermo Fisher Scientific Cat#17104019 TrypLE dissociation reagent Thermo Fisher Scientific Cat#12604013 B-27 supplement Thermo Fisher Scientific Cat#17504044 N2 supplement Thermo Fisher Scientific Cat#17502048 Matrigel coating reagent Thermo Fisher Scientific Cat#3432-005-01 Non-Essential Amino Acids (NEAA) supplement Thermo Fisher Scientific Cat#M7145 Laminin coating reagent Sigma Aldrich Cat#L2020 Geltrex coating reagent Thermo Fisher Scientific Cat#A1413201 (Continued on next page) Cell Stem Cell 28, 1–16.e1–e6, September 2, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pLVXUbC-rtTA-Ngn2:2A:Ascl1 (Herdy et al., 2019) Addgene #127289 pLVXUbC-rtTA-Ngn2:2A:EGFP (Schafer et al., 2019) Addgene #84777 pLVXTP-GcAMP6m This study N/A pCSC-hSyn1::dsRed (Mertens et al., 2015b) N/A pLVX-EF1a-tetOn-IRES-G418 (EtO) (Mertens et al., 2015a) Addgene #84776 Software and algorithms STAR Aligner https://github.com/alexdobin/STAR N/A Babraham Bioinformatics TrimGalore https://www.bioinformatics.babraham.ac.uk N/A Samtools samtools.github.io N/A Hypergeometric Optimization of Motif EnRichment (HOMER) homer.ucsd.edu N/A DESeq2 https://github.com/mikelove/DESeq2 N/A deepTools https://deeptools.readthedocs.io N/A bedtools https://bedtools.readthedocs.io N/A MBF Bioscience Neurolucida https://www.mbfbioscience.com/neurolucida N/A Integrative Genomics Viewer - Broad Institute (IGV) software.broadinstitute.org/software/igv N/A GraphPad Prism graphpad.com N/A FlowJo flowjo.com N/A STRING-db string-db.org N/A DAVID Functional Annotation https://david.ncifcrf.gov N/A Reduce + Visualize Gene Ontology (REVIGO) revigo.irb.hr N/A FACSChorus Software Becton Dickinson N/A ChAMP 10.18129/B9.bioc.ChAMP N/A RnBeads rnbeads.org N/A DNAmAGE http://dnamage.genetics.ucla.edu N/A Rank Rank Hypergeometric Overlap (RRHO) 10.18129/B9.bioc.RRHO N/A ZEN Imaging Software Carl Zeiss N/A ImageJ https://imagej.nih.gov/ij N/A ..

    Generated:

    Article Title: Designed NGF mimetics with reduced nociceptive signatures in neurons
    Article Snippet: Cells were passaged every 3-4 days using trypsin-EDTA (Gibco, Cat #25300054), maintained at 37°C in a humidified incubator and routinely tested for mycoplasma. .. For conversion to neurons, stable lines were generated by transducing two lentiviruses produced in HEK293FT cells with packaging plasmids psPAX2 and pMD2.G following the Addgene protocol: The first one containing pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A), a gift from Fred Gage (Addgene plasmid # 84777), a the second one a tet-operator plasmid (Addgene # 84776), followed by 4 days of puromycin (1μg/ml) and G418 (150 μg/ml) selection. ..

    Produced:

    Article Title: Designed NGF mimetics with reduced nociceptive signatures in neurons
    Article Snippet: Cells were passaged every 3-4 days using trypsin-EDTA (Gibco, Cat #25300054), maintained at 37°C in a humidified incubator and routinely tested for mycoplasma. .. For conversion to neurons, stable lines were generated by transducing two lentiviruses produced in HEK293FT cells with packaging plasmids psPAX2 and pMD2.G following the Addgene protocol: The first one containing pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A), a gift from Fred Gage (Addgene plasmid # 84777), a the second one a tet-operator plasmid (Addgene # 84776), followed by 4 days of puromycin (1μg/ml) and G418 (150 μg/ml) selection. ..

    Selection:

    Article Title: Designed NGF mimetics with reduced nociceptive signatures in neurons
    Article Snippet: Cells were passaged every 3-4 days using trypsin-EDTA (Gibco, Cat #25300054), maintained at 37°C in a humidified incubator and routinely tested for mycoplasma. .. For conversion to neurons, stable lines were generated by transducing two lentiviruses produced in HEK293FT cells with packaging plasmids psPAX2 and pMD2.G following the Addgene protocol: The first one containing pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A), a gift from Fred Gage (Addgene plasmid # 84777), a the second one a tet-operator plasmid (Addgene # 84776), followed by 4 days of puromycin (1μg/ml) and G418 (150 μg/ml) selection. ..



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    Macklin Inc eto
    Inhibition of the mFAO sensitizes muscle to atrophic stress. (a) qRT-PCR analysis of zebrafish larvae treated with DEXA(S) and/or <t>etomoxir</t> <t>(ETO)</t> showing the expression of the atrophy genes fbxo32 and trim63a (DEXA(S) indicates a short-course DEXA treatment. * p < 0.05, t-test, two-tailed, error bar indicates s.d.). (b) Representative fluorescent images of LysoView-stained zebrafish larvae treated with DEXA(S) and/or ETO. Histogram showing quantification of fluorescent intensity (white rectangle indicates the area chosen for the fluorescence quantification. Scale bar represents 1 mm. * p < 0.05, one-way ANOVA and Tukey’s multiple comparison test; error bar indicates s.d.). (c) Representative confocal microscopy images of Alexa488-phalloidin-stained zebrafish larvae treated with DEXA(S) and/or ETO with or without the hormesis treatment (arrows indicate examples of detached muscle fibers). Boxplot shows quantification of larvae with 0, 1–2, 3–15, or >15 detached muscle fibers (* p < 0.05, Chi-square test. Scale bar represents 50 µM).
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    Inhibition of the mFAO sensitizes muscle to atrophic stress. (a) qRT-PCR analysis of zebrafish larvae treated with DEXA(S) and/or etomoxir (ETO) showing the expression of the atrophy genes fbxo32 and trim63a (DEXA(S) indicates a short-course DEXA treatment. * p < 0.05, t-test, two-tailed, error bar indicates s.d.). (b) Representative fluorescent images of LysoView-stained zebrafish larvae treated with DEXA(S) and/or ETO. Histogram showing quantification of fluorescent intensity (white rectangle indicates the area chosen for the fluorescence quantification. Scale bar represents 1 mm. * p < 0.05, one-way ANOVA and Tukey’s multiple comparison test; error bar indicates s.d.). (c) Representative confocal microscopy images of Alexa488-phalloidin-stained zebrafish larvae treated with DEXA(S) and/or ETO with or without the hormesis treatment (arrows indicate examples of detached muscle fibers). Boxplot shows quantification of larvae with 0, 1–2, 3–15, or >15 detached muscle fibers (* p < 0.05, Chi-square test. Scale bar represents 50 µM).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Sirt1 coordinates the mitochondrial UPR and myocellular proteostasis to preserve muscle integrity during muscle atrophy in zebrafish

    doi: 10.3389/fcell.2026.1761278

    Figure Lengend Snippet: Inhibition of the mFAO sensitizes muscle to atrophic stress. (a) qRT-PCR analysis of zebrafish larvae treated with DEXA(S) and/or etomoxir (ETO) showing the expression of the atrophy genes fbxo32 and trim63a (DEXA(S) indicates a short-course DEXA treatment. * p < 0.05, t-test, two-tailed, error bar indicates s.d.). (b) Representative fluorescent images of LysoView-stained zebrafish larvae treated with DEXA(S) and/or ETO. Histogram showing quantification of fluorescent intensity (white rectangle indicates the area chosen for the fluorescence quantification. Scale bar represents 1 mm. * p < 0.05, one-way ANOVA and Tukey’s multiple comparison test; error bar indicates s.d.). (c) Representative confocal microscopy images of Alexa488-phalloidin-stained zebrafish larvae treated with DEXA(S) and/or ETO with or without the hormesis treatment (arrows indicate examples of detached muscle fibers). Boxplot shows quantification of larvae with 0, 1–2, 3–15, or >15 detached muscle fibers (* p < 0.05, Chi-square test. Scale bar represents 50 µM).

    Article Snippet: To block Cpt1 activity, larval zebrafish at 4 dpf were treated with 10 μM etomoxir (ETO) (Selleckchem) with or without DEXA for 18 h–20 h. To suppress autophagy, 5 mM chloroquine (Sigma) was added to the larval zebrafish medium for 1-h incubation.

    Techniques: Inhibition, Quantitative RT-PCR, Expressing, Two Tailed Test, Staining, Fluorescence, Comparison, Confocal Microscopy