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anti her2 erbb2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti her2 erbb2
    Anti Her2 Erbb2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/erbb2/HER2%2FErbB2+Rabbit+mAb/pmc13041752-4-0-2
    Average 96 stars, based on 489 article reviews
    anti her2 erbb2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Tissue-specific inflammation induces cell state plasticity with oncogenic addiction in mucosal melanoma
    Article Snippet: Anti-HER2/ErbB2 , Cell Signaling , 2165.

    Article Title: An MIEN1-based hexamer peptide (LA3IK) inhibits EGF-driven oncogenic signaling in prostate cancer by disrupting EGFR-ERBB2 heterodimerization.
    Article Snippet: A mousespecific monoclonal antibody against EGFR (Cat# 2256S) and a human-specific HER2/ErbB2 (29D8) rabbit monoclonal antibody (Cat# 2165S), both from Cell Signaling Technology, were used to distinguish the closely migrating protein bands of EGFR (~175 kDa) and ERBB2 (~185 kDa).

    Incubation:

    Article Title: A compatible gravity-driven organoid perfusion (GDOP) platform for drug screening with sensitivity and toxicity process evaluation.
    Article Snippet: .. Subsequently, tissue sections were incubated with the primary antibody (mouse anti-Ki-67 mAb (9449S, CST, 1:800), rabbit anti-ER mAb (ab16660, Abcam, 1:200), rabbit anti-PR mAb (ab101688, Abcam, 1:200) and rabbit anti-HER2 mAb (2165, CST, 1:200)) for 30 min at ambient temperature followed by a HRP-labeled goat anti-rabbit IgG or goat anti-mouse IgG coupled with alkaline phosphatase (30 min). ..

    Article Title: Quantitative HER2 tissue and plasma profiling predicts the activity of trastuzumab deruxtecan for breast cancer
    Article Snippet: .. The Leica BOND Rx autostainer protocol is as follows: deparaffinization with BOND dewax solution (AR9222), antigen retrieval with BOND HIER epitope retrieval solution 2 (AR9640) at 97 ° C for 20 min, blocking with ReadyProbes Endogenous HRP & AP blocking solution ( R37629 , Invitrogen) for 10 min and with BSA for 30 min, 1 h incubation with primary rabbit monoclonal HER2 antibody (clone 29D8, #2165, IgG, Cell Signaling) at optimal concentration of 1ug/ml mixed together with 1:100 concentration of pan-CK (Clones AE1/AE3, REF#M3515, Dako), amplification with Rabbit Envision+ System–HRP labeled polymer anti-Rabbit (K400311-2, Dako) mixed together with 1:100 dilution of a green-fluorescent Alexa Fluor 546 Goat-anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody (REF#A11003) for 1 h, staining with 1:50 dilution of a red-fluorescent Tyramide Signal Amplification (TSA) Cyanin 5 (SAT705A001EA, Akoya Biosciences) for 10 min and nuclear staining with 1:500 dilution of a blue-fluorescent 4′,6-diamidino-2-phenylindole (DAPI) for 10 min. .. Subsequently, the excess DI water on each slide was wiped off carefully and coverslipped with ProLong Gold Antifade mounting reagent ( P36930 , Invitrogen).

    Blocking Assay:

    Article Title: Quantitative HER2 tissue and plasma profiling predicts the activity of trastuzumab deruxtecan for breast cancer
    Article Snippet: .. The Leica BOND Rx autostainer protocol is as follows: deparaffinization with BOND dewax solution (AR9222), antigen retrieval with BOND HIER epitope retrieval solution 2 (AR9640) at 97 ° C for 20 min, blocking with ReadyProbes Endogenous HRP & AP blocking solution ( R37629 , Invitrogen) for 10 min and with BSA for 30 min, 1 h incubation with primary rabbit monoclonal HER2 antibody (clone 29D8, #2165, IgG, Cell Signaling) at optimal concentration of 1ug/ml mixed together with 1:100 concentration of pan-CK (Clones AE1/AE3, REF#M3515, Dako), amplification with Rabbit Envision+ System–HRP labeled polymer anti-Rabbit (K400311-2, Dako) mixed together with 1:100 dilution of a green-fluorescent Alexa Fluor 546 Goat-anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody (REF#A11003) for 1 h, staining with 1:50 dilution of a red-fluorescent Tyramide Signal Amplification (TSA) Cyanin 5 (SAT705A001EA, Akoya Biosciences) for 10 min and nuclear staining with 1:500 dilution of a blue-fluorescent 4′,6-diamidino-2-phenylindole (DAPI) for 10 min. .. Subsequently, the excess DI water on each slide was wiped off carefully and coverslipped with ProLong Gold Antifade mounting reagent ( P36930 , Invitrogen).

    Concentration Assay:

    Article Title: Quantitative HER2 tissue and plasma profiling predicts the activity of trastuzumab deruxtecan for breast cancer
    Article Snippet: .. The Leica BOND Rx autostainer protocol is as follows: deparaffinization with BOND dewax solution (AR9222), antigen retrieval with BOND HIER epitope retrieval solution 2 (AR9640) at 97 ° C for 20 min, blocking with ReadyProbes Endogenous HRP & AP blocking solution ( R37629 , Invitrogen) for 10 min and with BSA for 30 min, 1 h incubation with primary rabbit monoclonal HER2 antibody (clone 29D8, #2165, IgG, Cell Signaling) at optimal concentration of 1ug/ml mixed together with 1:100 concentration of pan-CK (Clones AE1/AE3, REF#M3515, Dako), amplification with Rabbit Envision+ System–HRP labeled polymer anti-Rabbit (K400311-2, Dako) mixed together with 1:100 dilution of a green-fluorescent Alexa Fluor 546 Goat-anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody (REF#A11003) for 1 h, staining with 1:50 dilution of a red-fluorescent Tyramide Signal Amplification (TSA) Cyanin 5 (SAT705A001EA, Akoya Biosciences) for 10 min and nuclear staining with 1:500 dilution of a blue-fluorescent 4′,6-diamidino-2-phenylindole (DAPI) for 10 min. .. Subsequently, the excess DI water on each slide was wiped off carefully and coverslipped with ProLong Gold Antifade mounting reagent ( P36930 , Invitrogen).

    Amplification:

    Article Title: Quantitative HER2 tissue and plasma profiling predicts the activity of trastuzumab deruxtecan for breast cancer
    Article Snippet: .. The Leica BOND Rx autostainer protocol is as follows: deparaffinization with BOND dewax solution (AR9222), antigen retrieval with BOND HIER epitope retrieval solution 2 (AR9640) at 97 ° C for 20 min, blocking with ReadyProbes Endogenous HRP & AP blocking solution ( R37629 , Invitrogen) for 10 min and with BSA for 30 min, 1 h incubation with primary rabbit monoclonal HER2 antibody (clone 29D8, #2165, IgG, Cell Signaling) at optimal concentration of 1ug/ml mixed together with 1:100 concentration of pan-CK (Clones AE1/AE3, REF#M3515, Dako), amplification with Rabbit Envision+ System–HRP labeled polymer anti-Rabbit (K400311-2, Dako) mixed together with 1:100 dilution of a green-fluorescent Alexa Fluor 546 Goat-anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody (REF#A11003) for 1 h, staining with 1:50 dilution of a red-fluorescent Tyramide Signal Amplification (TSA) Cyanin 5 (SAT705A001EA, Akoya Biosciences) for 10 min and nuclear staining with 1:500 dilution of a blue-fluorescent 4′,6-diamidino-2-phenylindole (DAPI) for 10 min. .. Subsequently, the excess DI water on each slide was wiped off carefully and coverslipped with ProLong Gold Antifade mounting reagent ( P36930 , Invitrogen).

    Labeling:

    Article Title: Quantitative HER2 tissue and plasma profiling predicts the activity of trastuzumab deruxtecan for breast cancer
    Article Snippet: .. The Leica BOND Rx autostainer protocol is as follows: deparaffinization with BOND dewax solution (AR9222), antigen retrieval with BOND HIER epitope retrieval solution 2 (AR9640) at 97 ° C for 20 min, blocking with ReadyProbes Endogenous HRP & AP blocking solution ( R37629 , Invitrogen) for 10 min and with BSA for 30 min, 1 h incubation with primary rabbit monoclonal HER2 antibody (clone 29D8, #2165, IgG, Cell Signaling) at optimal concentration of 1ug/ml mixed together with 1:100 concentration of pan-CK (Clones AE1/AE3, REF#M3515, Dako), amplification with Rabbit Envision+ System–HRP labeled polymer anti-Rabbit (K400311-2, Dako) mixed together with 1:100 dilution of a green-fluorescent Alexa Fluor 546 Goat-anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody (REF#A11003) for 1 h, staining with 1:50 dilution of a red-fluorescent Tyramide Signal Amplification (TSA) Cyanin 5 (SAT705A001EA, Akoya Biosciences) for 10 min and nuclear staining with 1:500 dilution of a blue-fluorescent 4′,6-diamidino-2-phenylindole (DAPI) for 10 min. .. Subsequently, the excess DI water on each slide was wiped off carefully and coverslipped with ProLong Gold Antifade mounting reagent ( P36930 , Invitrogen).

    Polymer:

    Article Title: Quantitative HER2 tissue and plasma profiling predicts the activity of trastuzumab deruxtecan for breast cancer
    Article Snippet: .. The Leica BOND Rx autostainer protocol is as follows: deparaffinization with BOND dewax solution (AR9222), antigen retrieval with BOND HIER epitope retrieval solution 2 (AR9640) at 97 ° C for 20 min, blocking with ReadyProbes Endogenous HRP & AP blocking solution ( R37629 , Invitrogen) for 10 min and with BSA for 30 min, 1 h incubation with primary rabbit monoclonal HER2 antibody (clone 29D8, #2165, IgG, Cell Signaling) at optimal concentration of 1ug/ml mixed together with 1:100 concentration of pan-CK (Clones AE1/AE3, REF#M3515, Dako), amplification with Rabbit Envision+ System–HRP labeled polymer anti-Rabbit (K400311-2, Dako) mixed together with 1:100 dilution of a green-fluorescent Alexa Fluor 546 Goat-anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody (REF#A11003) for 1 h, staining with 1:50 dilution of a red-fluorescent Tyramide Signal Amplification (TSA) Cyanin 5 (SAT705A001EA, Akoya Biosciences) for 10 min and nuclear staining with 1:500 dilution of a blue-fluorescent 4′,6-diamidino-2-phenylindole (DAPI) for 10 min. .. Subsequently, the excess DI water on each slide was wiped off carefully and coverslipped with ProLong Gold Antifade mounting reagent ( P36930 , Invitrogen).

    Staining:

    Article Title: Quantitative HER2 tissue and plasma profiling predicts the activity of trastuzumab deruxtecan for breast cancer
    Article Snippet: .. The Leica BOND Rx autostainer protocol is as follows: deparaffinization with BOND dewax solution (AR9222), antigen retrieval with BOND HIER epitope retrieval solution 2 (AR9640) at 97 ° C for 20 min, blocking with ReadyProbes Endogenous HRP & AP blocking solution ( R37629 , Invitrogen) for 10 min and with BSA for 30 min, 1 h incubation with primary rabbit monoclonal HER2 antibody (clone 29D8, #2165, IgG, Cell Signaling) at optimal concentration of 1ug/ml mixed together with 1:100 concentration of pan-CK (Clones AE1/AE3, REF#M3515, Dako), amplification with Rabbit Envision+ System–HRP labeled polymer anti-Rabbit (K400311-2, Dako) mixed together with 1:100 dilution of a green-fluorescent Alexa Fluor 546 Goat-anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody (REF#A11003) for 1 h, staining with 1:50 dilution of a red-fluorescent Tyramide Signal Amplification (TSA) Cyanin 5 (SAT705A001EA, Akoya Biosciences) for 10 min and nuclear staining with 1:500 dilution of a blue-fluorescent 4′,6-diamidino-2-phenylindole (DAPI) for 10 min. .. Subsequently, the excess DI water on each slide was wiped off carefully and coverslipped with ProLong Gold Antifade mounting reagent ( P36930 , Invitrogen).



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