anti phospho her2 erbb2  (Cell Signaling Technology Inc)

Journal: Advanced Science

Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21

doi: 10.1002/advs.78290

Figure Lengend Snippet:

Article Snippet: HER2 , 2165S , Cell Signaling Technology (CST) , AB_10692490.

Techniques: Cell-Signaling

Journal: Bioengineering & Translational Medicine

Article Title: Combined HER2 ‐targeted mRNA ‐ LNP vaccine and radiotherapy suppress Lewis lung carcinoma growth in mice: Efficacy and mechanistic insights

doi: 10.1002/btm2.70160

Figure Lengend Snippet: (a) Plasmid map of the HER2 extracellular domain (ECD). (b) Flowchart of HER2‐mRNA‐LNP preparation. (c) Agarose gel electrophoresis of HER2 ECD mRNA (double‐site digested product). (d) Agarose gel electrophoresis of HER2 ECD total mRNA (single‐site digested product). (e) Particle size and zeta potential of blank LNPs and HER2‐mRNA‐LNPs. (f) Representative cryo‐TEM micrographs of HER2‐mRNA‐LNPs at magnifications of 28,000×, 45,000×, and 92,000×. (g) Encapsulation efficiency of HER2‐mRNA‐LNPs. (h) Western blot analysis of HER2 ECD protein expression, with relative grayscale values quantified and statistically analyzed by Student's t ‐test ( n = 3 independent experiments). (i) Serum anti‐HER2 antibody titers in BALB/c and C57BL/6 mice immunized with LNP or HER2‐mRNA‐LNP after three doses. Data are presented as mean ± SEM. n = 3 mice per group. Statistical significance was determined by two‐way repeated‐measures ANOVA followed by Tukey's multiple comparisons test. **** p < 0.0001.

Article Snippet: Human HER2 ECD (Sino Biological; 0.25 mg/mL) was coated onto an ELISA plate overnight at 20 ng/well.

Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Zeta Potential Analyzer, Cryo-TEM, Encapsulation, Western Blot, Analysis, Expressing

Journal: Bioengineering & Translational Medicine

Article Title: Combined HER2 ‐targeted mRNA ‐ LNP vaccine and radiotherapy suppress Lewis lung carcinoma growth in mice: Efficacy and mechanistic insights

doi: 10.1002/btm2.70160

Figure Lengend Snippet: (a) Gross images of liver, lung, heart, and spleen from mice treated with LNP or HER2‐mRNA‐LNP. (b) Body weight and organ weights (liver, lung, heart, and spleen) of mice in the two groups. (c) H&E staining of liver, lung, heart and spleen tissue sections (magnification: 200× and 400×). (d) Peripheral blood routine analysis: white blood cell (WBC), red blood cell (RBC), and platelet (PLT) counts. (e) Serum liver function parameters: alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), γ‐glutamyl transpeptidase (γ‐GT), albumin (ALB), direct bilirubin (DBIL), total bilirubin (TBIL), and total bile acid (TBA). (f) Serum renal function parameters: Urea (UREA), creatinine (CREA), and uric acid (UA). (g) Serum myocardial enzyme profile: creatine kinase (CK (U/L)), creatine kinase‐MB (CK‐MB), lactate dehydrogenase (LDH), and lactate dehydrogenase isoenzyme 1 (LDH1). All data are presented as mean ± SEM, with n = 5 mice per group. Statistical analysis was performed using unpaired two‐tailed Student's t ‐test. ** p < 0.01; ns, not significant ( p > 0.05).

Article Snippet: Human HER2 ECD (Sino Biological; 0.25 mg/mL) was coated onto an ELISA plate overnight at 20 ng/well.

Techniques: Staining, Tissue, Analysis, Two Tailed Test

Journal: Bioengineering & Translational Medicine

Article Title: Combined HER2 ‐targeted mRNA ‐ LNP vaccine and radiotherapy suppress Lewis lung carcinoma growth in mice: Efficacy and mechanistic insights

doi: 10.1002/btm2.70160

Figure Lengend Snippet: (a) HER2 mRNA expression in LLC and HER2‐LLC cells detected by Q‐PCR; HER2 protein expression in LLC, HER2‐LLC, SKBR3, and A549 cells analyzed by Western blot. (b) Anti‐HER2 antibody titers in mice after three doses of HER2‐mRNA‐LNP. (c) Tumor growth curve of HER2‐LLC tumor‐bearing mice in each group. (d) Treatment schedule for each group of mice. (e) Representative tumor images from each group. (f) Tumor volume and weight in each group. (g) CCK‐8 assay‐based inhibition rates of HER2‐LLC cells by splenic lymphocytes from tumor‐bearing mice. (h) Correlation analysis of tumor volume and antibody titer in HER2‐mRNA‐LNP group mice. (i) Quantitative immunohistochemical analysis of HER2 and Ki67 expression in tumor tissues from each group of mice. Three fields of view were selected per mouse, yielding 15 data points per group. (j) Representative immunohistochemical images of HER2 and Ki67 expression in mouse tumor tissues (magnification: 200× and 400×). (k) Kinetics of antibody titers over time in the PBS group, 1‐dose vaccine group, and 3‐dose vaccine group. All data are presented as mean ± SEM, with n = 5 mice per group. One‐way ANOVA with Tukey's test or Kruskal–Wallis test with Dunn's test was used for multiple group comparisons, as appropriate; statistical analysis was performed using two‐way repeated‐measures ANOVA followed by Tukey's multiple comparisons test for antibody titers, tumor growth curves and CCK‐8 inhibition rates; Pearson correlation analysis for correlation analyses ( R = −0.8889, p = 0.0437). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).

Article Snippet: Human HER2 ECD (Sino Biological; 0.25 mg/mL) was coated onto an ELISA plate overnight at 20 ng/well.

Techniques: Expressing, Western Blot, CCK-8 Assay, Inhibition, Analysis, Titer Analysis, Immunohistochemical staining

Journal: Bioengineering & Translational Medicine

Article Title: Combined HER2 ‐targeted mRNA ‐ LNP vaccine and radiotherapy suppress Lewis lung carcinoma growth in mice: Efficacy and mechanistic insights

doi: 10.1002/btm2.70160

Figure Lengend Snippet: Results of two‐way repeated‐measures ANOVA for serum HER2 antibody titer levels.

Article Snippet: Human HER2 ECD (Sino Biological; 0.25 mg/mL) was coated onto an ELISA plate overnight at 20 ng/well.

Techniques: Titer Analysis

Journal: Bioengineering & Translational Medicine

Article Title: Combined HER2 ‐targeted mRNA ‐ LNP vaccine and radiotherapy suppress Lewis lung carcinoma growth in mice: Efficacy and mechanistic insights

doi: 10.1002/btm2.70160

Figure Lengend Snippet: (a) Experimental timeline for each group. (b) Tumor growth curves of HER2‐LLC tumor‐bearing mice. (c) Representative tumor images. (d) Quantification of tumor volume and weight ( n = 5). (e) CCK‐8 assay‐based cytotoxicity inhibition rate at 48 h ( n = 3). (f) Representative immunohistochemical images of HER2 and Ki67 expression in mouse tumor tissues. (g) Quantitative analysis of HER2 and Ki67 immunohistochemical staining across groups (Three random fields per mouse were selected for analysis. n = 5). All data are presented as mean ± SEM. Two‐way repeated‐measures ANOVA with Tukey's test was used for tumor growth curves ( n = 5) (b); one‐way ANOVA with Tukey's test was used for multiple group comparisons (d,e,g).

Article Snippet: Human HER2 ECD (Sino Biological; 0.25 mg/mL) was coated onto an ELISA plate overnight at 20 ng/well.

Techniques: CCK-8 Assay, Inhibition, Immunohistochemical staining, Expressing, Analysis, Staining