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er tr7  (Hycult Biotech)


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    Hycult Biotech er tr7
    Er Tr7, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/er+tr7/Fibroblasts%2C+Mouse%2C+mAb+ER-TR7/pmc03633981-69-40-44
    Average 90 stars, based on 3 article reviews
    er tr7 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Peripherally Administered Nanoparticles Target Monocytic Myeloid Cells, Secondary Lymphoid Organs and Tumors in Mice
    Article Snippet: .. LN sections were either stained for hematoxylin and eosin, or immunostained for (i) CD35 (biotinylated rabbit anti-mouse, clone 8C12, BD Pharmingen, Franklin Lakes, NJ US) with streptavidin-A488 (Invitrogen) and DAPI, or (ii) Lyve-1 (rabbit anti-mouse, 103-PA50AG, ReliaTech, Wolfenbüttel, Germany) and ER-TR7 (rat anti-human/mouse, HM1086, Hycult biotech, Uden, The Netherlands) with secondary antibodies, A488-rabbit anti-mouse IgG and A546-goat anti-rat,IgG, respectively (Invitrogen). .. Images were taken on a Zeiss LSM 710 confocal microscope (Carl Zeiss Inc., Oberkochen, Germany) and in an upright Leica DM5500 (Leica Microsystems, Bensheim, Germany).

    Immunofluorescence:

    Article Title: Elevated H3K27ac in aged skeletal muscle leads to increase in extracellular matrix and fibrogenic conversion of muscle satellite cells
    Article Snippet: .. Immunofluorescence (IF) on cells was performed using the following antibodies: MyHC (Developmental Studies Hybridoma Bank, 1:50), Myogenin (Santa Cruz Biotechnology; 1:350), and ERTR7 (Hycult Biotech, HM1086; 1:20). ..

    Article Title: Elevated H3K27ac in aged skeletal muscle leads to increase in extracellular matrix and fibrogenic conversion of muscle satellite cells.
    Article Snippet: .. Immunofluorescence (IF) on cells was performed using the following antibodies: MyHC (Developmental Studies Hybridoma Bank, 1:50), Myogenin (Santa Cruz Biotechnology; 1:350), and ERTR7 (Hycult Biotech, HM1086; 1:20). ..

    other:

    Article Title: Tumor Induced Stromal Reprogramming Drives Lymph Node Transformation
    Article Snippet: Rat anti-mouse fibroblast , ER-TR7 , Hycult Biotech , HM1086 , 1:50 , http://www.hycultbiotech.com/hm1086.



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    a Schematic of mRNA-miR vaccine constructs encoding influenza NP or HA, followed by miR-targeting sequences in series. b Schematic of expected antigen expression patterns and relevant antigen processing pathways for each mRNA-miR construct. c, d Representative histograms, gated on live singlets ( c ), and summary data ( d ) of %NP-expressing cells by flow cytometry after in vitro transfection with NP-miR mRNA. e–g NP expression in murine lymph nodes after i.m. immunization with mRNA-LNPs. e Representative composite images (top row) and magnified insets (bottom row) of inguinal lymph <t>nodes.</t> <t>ER-TR7</t> is a fibroblastic reticular cell marker. Scale bars indicate 100 μm. f Surface area of positive NP staining, relative to the entire lymph node surface area. g Integrated intensity of NP staining among all NP + pixels. f , g Bars indicate mean of 3 biological replicates +SEM. Each symbol represents one mouse. Analyzed by 1-way ANOVA; results of Dunnett’s multiple comparison test are shown. h Representative western blot after transfection of C2C12 myotubes with NP-miR mRNA. i Summary data of NP expression as in ( h ), quantified relative to GAPDH using densitometry analysis. d , i Bars represent mean + SEM of biological replicates pooled from 3 ( d ) or 4 ( i ) independent experiments and normalized to the average %NP + value ( d ) or average NP/GAPDH value ( i ) among NP-control-transfected cells, per cell type within each experiment. Sample sizes: fibroblasts n = 7 (NP-control) or n = 8 (NP-miR142t, NP-miR206t) ( d ); DC2.4 n = 5 per condition ( d ); BMDC n = 4 per condition ( d ); C2C12 n = 10 per condition ( i ). d , i Analyzed by linear mixed-effects model with cell type ( d ) and mRNA construct ( d , i ) treated as fixed effects and experiment treated as a random effect ( d , i ). Significance of pairwise comparisons to control mRNA, determined by likelihood ratio test followed by Dunnett’s multiple comparison test within each cell type, are shown. d Among DC2.4, NP-control vs. NP-miR142t p < 1 × 10 -10 and NP-control vs. NP-miR206t p = 3.51 × 10 -7 ; among BMDC, NP-control vs. NP-miR142t p < 1 × 10 -10 and NP-control vs. NP-miR206t p = 1.1 × 10 -8 . i NP-control vs. NP-miR206t p < 1 × 10 -10 . c , h PR8 influenza-infected and untreated controls are included for comparison. d , f – i Source data are provided as a Source Data file.
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    a Schematic of mRNA-miR vaccine constructs encoding influenza NP or HA, followed by miR-targeting sequences in series. b Schematic of expected antigen expression patterns and relevant antigen processing pathways for each mRNA-miR construct. c, d Representative histograms, gated on live singlets ( c ), and summary data ( d ) of %NP-expressing cells by flow cytometry after in vitro transfection with NP-miR mRNA. e–g NP expression in murine lymph nodes after i.m. immunization with mRNA-LNPs. e Representative composite images (top row) and magnified insets (bottom row) of inguinal lymph <t>nodes.</t> <t>ER-TR7</t> is a fibroblastic reticular cell marker. Scale bars indicate 100 μm. f Surface area of positive NP staining, relative to the entire lymph node surface area. g Integrated intensity of NP staining among all NP + pixels. f , g Bars indicate mean of 3 biological replicates +SEM. Each symbol represents one mouse. Analyzed by 1-way ANOVA; results of Dunnett’s multiple comparison test are shown. h Representative western blot after transfection of C2C12 myotubes with NP-miR mRNA. i Summary data of NP expression as in ( h ), quantified relative to GAPDH using densitometry analysis. d , i Bars represent mean + SEM of biological replicates pooled from 3 ( d ) or 4 ( i ) independent experiments and normalized to the average %NP + value ( d ) or average NP/GAPDH value ( i ) among NP-control-transfected cells, per cell type within each experiment. Sample sizes: fibroblasts n = 7 (NP-control) or n = 8 (NP-miR142t, NP-miR206t) ( d ); DC2.4 n = 5 per condition ( d ); BMDC n = 4 per condition ( d ); C2C12 n = 10 per condition ( i ). d , i Analyzed by linear mixed-effects model with cell type ( d ) and mRNA construct ( d , i ) treated as fixed effects and experiment treated as a random effect ( d , i ). Significance of pairwise comparisons to control mRNA, determined by likelihood ratio test followed by Dunnett’s multiple comparison test within each cell type, are shown. d Among DC2.4, NP-control vs. NP-miR142t p < 1 × 10 -10 and NP-control vs. NP-miR206t p = 3.51 × 10 -7 ; among BMDC, NP-control vs. NP-miR142t p < 1 × 10 -10 and NP-control vs. NP-miR206t p = 1.1 × 10 -8 . i NP-control vs. NP-miR206t p < 1 × 10 -10 . c , h PR8 influenza-infected and untreated controls are included for comparison. d , f – i Source data are provided as a Source Data file.
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    Image Search Results


    a Schematic of mRNA-miR vaccine constructs encoding influenza NP or HA, followed by miR-targeting sequences in series. b Schematic of expected antigen expression patterns and relevant antigen processing pathways for each mRNA-miR construct. c, d Representative histograms, gated on live singlets ( c ), and summary data ( d ) of %NP-expressing cells by flow cytometry after in vitro transfection with NP-miR mRNA. e–g NP expression in murine lymph nodes after i.m. immunization with mRNA-LNPs. e Representative composite images (top row) and magnified insets (bottom row) of inguinal lymph nodes. ER-TR7 is a fibroblastic reticular cell marker. Scale bars indicate 100 μm. f Surface area of positive NP staining, relative to the entire lymph node surface area. g Integrated intensity of NP staining among all NP + pixels. f , g Bars indicate mean of 3 biological replicates +SEM. Each symbol represents one mouse. Analyzed by 1-way ANOVA; results of Dunnett’s multiple comparison test are shown. h Representative western blot after transfection of C2C12 myotubes with NP-miR mRNA. i Summary data of NP expression as in ( h ), quantified relative to GAPDH using densitometry analysis. d , i Bars represent mean + SEM of biological replicates pooled from 3 ( d ) or 4 ( i ) independent experiments and normalized to the average %NP + value ( d ) or average NP/GAPDH value ( i ) among NP-control-transfected cells, per cell type within each experiment. Sample sizes: fibroblasts n = 7 (NP-control) or n = 8 (NP-miR142t, NP-miR206t) ( d ); DC2.4 n = 5 per condition ( d ); BMDC n = 4 per condition ( d ); C2C12 n = 10 per condition ( i ). d , i Analyzed by linear mixed-effects model with cell type ( d ) and mRNA construct ( d , i ) treated as fixed effects and experiment treated as a random effect ( d , i ). Significance of pairwise comparisons to control mRNA, determined by likelihood ratio test followed by Dunnett’s multiple comparison test within each cell type, are shown. d Among DC2.4, NP-control vs. NP-miR142t p < 1 × 10 -10 and NP-control vs. NP-miR206t p = 3.51 × 10 -7 ; among BMDC, NP-control vs. NP-miR142t p < 1 × 10 -10 and NP-control vs. NP-miR206t p = 1.1 × 10 -8 . i NP-control vs. NP-miR206t p < 1 × 10 -10 . c , h PR8 influenza-infected and untreated controls are included for comparison. d , f – i Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Optimal murine CD4 + T cell priming by mRNA-lipid nanoparticle vaccines requires endogenous antigen processing

    doi: 10.1038/s41467-025-68080-x

    Figure Lengend Snippet: a Schematic of mRNA-miR vaccine constructs encoding influenza NP or HA, followed by miR-targeting sequences in series. b Schematic of expected antigen expression patterns and relevant antigen processing pathways for each mRNA-miR construct. c, d Representative histograms, gated on live singlets ( c ), and summary data ( d ) of %NP-expressing cells by flow cytometry after in vitro transfection with NP-miR mRNA. e–g NP expression in murine lymph nodes after i.m. immunization with mRNA-LNPs. e Representative composite images (top row) and magnified insets (bottom row) of inguinal lymph nodes. ER-TR7 is a fibroblastic reticular cell marker. Scale bars indicate 100 μm. f Surface area of positive NP staining, relative to the entire lymph node surface area. g Integrated intensity of NP staining among all NP + pixels. f , g Bars indicate mean of 3 biological replicates +SEM. Each symbol represents one mouse. Analyzed by 1-way ANOVA; results of Dunnett’s multiple comparison test are shown. h Representative western blot after transfection of C2C12 myotubes with NP-miR mRNA. i Summary data of NP expression as in ( h ), quantified relative to GAPDH using densitometry analysis. d , i Bars represent mean + SEM of biological replicates pooled from 3 ( d ) or 4 ( i ) independent experiments and normalized to the average %NP + value ( d ) or average NP/GAPDH value ( i ) among NP-control-transfected cells, per cell type within each experiment. Sample sizes: fibroblasts n = 7 (NP-control) or n = 8 (NP-miR142t, NP-miR206t) ( d ); DC2.4 n = 5 per condition ( d ); BMDC n = 4 per condition ( d ); C2C12 n = 10 per condition ( i ). d , i Analyzed by linear mixed-effects model with cell type ( d ) and mRNA construct ( d , i ) treated as fixed effects and experiment treated as a random effect ( d , i ). Significance of pairwise comparisons to control mRNA, determined by likelihood ratio test followed by Dunnett’s multiple comparison test within each cell type, are shown. d Among DC2.4, NP-control vs. NP-miR142t p < 1 × 10 -10 and NP-control vs. NP-miR206t p = 3.51 × 10 -7 ; among BMDC, NP-control vs. NP-miR142t p < 1 × 10 -10 and NP-control vs. NP-miR206t p = 1.1 × 10 -8 . i NP-control vs. NP-miR206t p < 1 × 10 -10 . c , h PR8 influenza-infected and untreated controls are included for comparison. d , f – i Source data are provided as a Source Data file.

    Article Snippet: Cryosections were taken at 16 μm (iLN) or 10 μm (muscle), dried, and stored at −80 °C until staining. iLN sections were permeabilized in 0.5% Triton X-100 for 15 min, blocked in 5% goat serum in PBS for 30 minutes, and stained overnight with Influenza A NP monoclonal antibody (Clone D67J) conjugated to FITC (Invitrogen MA1-7332, diluted 1:100) and Fibroblast Marker antibody (ER-TR7) conjugated to AF546 (Santa Cruz Biotechnology, sc-73355, diluted 1:50).

    Techniques: Construct, Expressing, Flow Cytometry, In Vitro, Transfection, Marker, Staining, Comparison, Western Blot, Control, Infection