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gift fromerik snapp  (Addgene inc)


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    Structured Review

    Addgene inc gift fromerik snapp
    Gift Fromerik Snapp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/er+mrfp/ER-mRFP+(Plasmid+%2362236)/pm39831730-64-4-7
    Average 93 stars, based on 12 article reviews
    gift fromerik snapp - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Targeting Cholesterol Biosynthesis with Statins Synergizes with AKT Inhibitors in Triple-Negative Breast Cancer
    Article Snippet: The following primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5′-CAG GAC ATC TTG TCA AAA GTG CCA TGA TTC ACA ACA GGT CGA-3′ (forward), 5′-TCG ACC TGT TGT GAA TCA TGG CAC TTT TGA CAA GAT GTC CTG-3′ (reverse), E559A: 5′-CCA ATG GCA ACA ACA GCA GGT TGT CTT GTG GCC-3′ (forward), 5′-GGC CAC AAG ACA ACC TGC TGT TGT TGC CAT TGG-3′ (reverse), K691A: 5′-GTT AGT GGT AAC TAT TGT ACT GAC GCG AAA CCT GCT GCT ATA AAT TGG AT-3′ (forward), 5′-ATC CAA TTT ATA GCA GCA GGT TTC GCG TCA GTA CAA TAG TTA CCA CTA AC-3′ (reverse), D767A: 5′-CAT TGC CTG TGG ACA GGC TGC AGC ACA GAA TGT TG-3′ (forward), 5′-CAA CAT TCT GTG CTG CAG CCT GTC CAC AGG CAA TG-3′ (reverse). .. To generate pLenti6-ER-mRFP, mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5′-GGT CGG ATC CGC CGC CAC CAT GGA CAG CAA AGG-3′ and the reverse primer 5′-GAG GCA CCG GTG TTT AGA GCT CAT CTT T-3′. .. PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) by following the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: Targeting Cholesterol Biosynthesis with Statins Synergizes with AKT Inhibitors in Triple-Negative Breast Cancer
    Article Snippet: The following primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5′-CAG GAC ATC TTG TCA AAA GTG CCA TGA TTC ACA ACA GGT CGA-3′ (forward), 5′-TCG ACC TGT TGT GAA TCA TGG CAC TTT TGA CAA GAT GTC CTG-3′ (reverse), E559A: 5′-CCA ATG GCA ACA ACA GCA GGT TGT CTT GTG GCC-3′ (forward), 5′-GGC CAC AAG ACA ACC TGC TGT TGT TGC CAT TGG-3′ (reverse), K691A: 5′-GTT AGT GGT AAC TAT TGT ACT GAC GCG AAA CCT GCT GCT ATA AAT TGG AT-3′ (forward), 5′-ATC CAA TTT ATA GCA GCA GGT TTC GCG TCA GTA CAA TAG TTA CCA CTA AC-3′ (reverse), D767A: 5′-CAT TGC CTG TGG ACA GGC TGC AGC ACA GAA TGT TG-3′ (forward), 5′-CAA CAT TCT GTG CTG CAG CCT GTC CAC AGG CAA TG-3′ (reverse). .. To generate pLenti6-ER-mRFP, mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5′-GGT CGG ATC CGC CGC CAC CAT GGA CAG CAA AGG-3′ and the reverse primer 5′-GAG GCA CCG GTG TTT AGA GCT CAT CTT T-3′. .. PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) by following the manufacturer’s protocol.

    Amplification:

    Article Title: Targeting Cholesterol Biosynthesis with Statins Synergizes with AKT Inhibitors in Triple-Negative Breast Cancer
    Article Snippet: The following primers were generated using Agilent’s QuikChange Primer Design tool: H866A: 5′-CAG GAC ATC TTG TCA AAA GTG CCA TGA TTC ACA ACA GGT CGA-3′ (forward), 5′-TCG ACC TGT TGT GAA TCA TGG CAC TTT TGA CAA GAT GTC CTG-3′ (reverse), E559A: 5′-CCA ATG GCA ACA ACA GCA GGT TGT CTT GTG GCC-3′ (forward), 5′-GGC CAC AAG ACA ACC TGC TGT TGT TGC CAT TGG-3′ (reverse), K691A: 5′-GTT AGT GGT AAC TAT TGT ACT GAC GCG AAA CCT GCT GCT ATA AAT TGG AT-3′ (forward), 5′-ATC CAA TTT ATA GCA GCA GGT TTC GCG TCA GTA CAA TAG TTA CCA CTA AC-3′ (reverse), D767A: 5′-CAT TGC CTG TGG ACA GGC TGC AGC ACA GAA TGT TG-3′ (forward), 5′-CAA CAT TCT GTG CTG CAG CCT GTC CAC AGG CAA TG-3′ (reverse). .. To generate pLenti6-ER-mRFP, mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5′-GGT CGG ATC CGC CGC CAC CAT GGA CAG CAA AGG-3′ and the reverse primer 5′-GAG GCA CCG GTG TTT AGA GCT CAT CTT T-3′. .. PCR-amplified ER-mRFP was cloned into pLenti6/V5-p53_wt p53 (gift from the Muranen lab) by double restriction enzyme digestion with BamHI-HF (New England Biolabs, R0136) and AgeI-HF (New England Biolabs, R3552) at 37°C for 1 hour, followed by ligation with T4 DNA ligase (New England Biolabs, M0202) by following the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: An X-Linked Ataxia Syndrome in a Family with Hearing Loss Associated with a Novel Variant in the BCAP31 Gene.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense mutant was performed with the designed primer (Forward: GTGGACTGCAaTTGCCACCTT Reverse: TGCAGACTCATGGCGATC) and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, E0552S). .. The plasmids ER-mRFPwas a gift fromErik Snapp (Addgene plasmid 62236; RRID:Addgene_62,236).22 .. SH-SY5Y, obtained from ATCC (CCL-2266 Homo sapiens neuroblastoma), were maintained in Dulbecco’s modified Eagle’s medium F12 (DMEM F12) supplemented with high glucose, 10% fetal bovine serum (FBS), 1% penicillin/streptomycin (final concentration 100 U/mL of penicillin and 100 μg/mL of streptomycin), 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 1 mM sodium pyruvate, 2 mM LGlutaMAX and 1 non-essential amino acids (NEAA) (all from Thermo Fisher Scientific, Waltham, MA) at 37 C, in 5% CO2 and split 1:10 every 4 to 7 days.

    Article Title: An X‐Linked Ataxia Syndrome in a Family with Hearing Loss Associated with a Novel Variant in the BCAP31 Gene
    Article Snippet: BCAP31‐tGFP mutant was performed with the designed primer (Forward: GTGGACTGCAaTTGCCACCTT Reverse: TGCAGACTCATGGCGATC) and the Q5 Site‐Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, E0552S). .. The plasmids ER‐mRFP was a gift from Erik Snapp (Addgene plasmid 62236; RRID: Addgene_62,236). .. SH‐SY5Y, obtained from ATCC (CCL‐2266 Homo sapiens neuroblastoma), were maintained in Dulbecco's modified Eagle's medium F12 (DMEM F12) supplemented with high glucose, 10% fetal bovine serum (FBS), 1% penicillin/streptomycin (final concentration 100 U/mL of penicillin and 100 μg/mL of streptomycin), 10 mM 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES), 1 mM sodium pyruvate, 2 mM L‐GlutaMAX and 1 × non‐essential amino acids (NEAA) (all from Thermo Fisher Scientific, Waltham, MA) at 37°C, in 5% CO 2 and split 1:10 every 4 to 7 days.



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