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Applichem inc equilibration buffer
Equilibration Buffer, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/equilibration+buffer/equilibration+buffer/pm25441713-199-15-27
Average 90 stars, based on 1 article reviews
equilibration buffer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Expressing:

Article Title: Viral potassium channels as a robust model system for studies of membrane-protein interaction.
Article Snippet: .. After the expression, the protein was loaded on a Ni–NTA column, which was equilibrated with an equilibration buffer [500 mM NaCl, 30 mM HEPES, 10% glycerin (all from AppliChem GmbH, Darmstadt, Germany), pH 7.5]. .. Unspecific binding was removed by washing the column with 20 mM imidazole (Sigma Chemical, Deisendorf, Germany) twice.

Article Title: Viruses infecting marine picoplancton encode functional potassium ion channels.
Article Snippet: .. After expression, the protein was loaded on a Ni–NTA column, which was equilibrated with an equilibration buffer [500 mM NaCl, 30 mM HEPES, 10% glycerin (all from AppliChem GmbH, Darmstadt, Germany), pH 7.5]. .. Unspecific binding was removed by washing the column with 20 mM imidazole (Sigma Chemical, Deisendorf, Germany) twice.

Stripping Membranes:

Article Title: VHL-dependent alterations in the secretome of renal cell carcinoma: Association with immune cell response?
Article Snippet: After 2 h of rehydration, isoelectric focusing (IEF) was carried out at 20°C on an Ettan IPGphor 2 unit (GE Healthcare) at the following settings: 30 V 10 hrs, 500 V 1 h, 1000 V 1 h, 5000 V 1 h, and 8000 V up to a total of 45,000 Vhrs. .. The IPG strips were subjected to a 2 step strip equilibration procedure, which was performed by incubation the strips first for 15 min in 12 ml equilibration buffer I (6 M urea, 2% SDS, 50 mM Tris HCl (pH 8.8), 30% glycerol, supplemented with 1.5% DTT) followed by 15 min in 12 ml equilibration buffer II (6 M urea, 2% SDS, 50 mM Tris HCl (pH 8.8), 30% glycerol, supplemented with 4.8% iodoacetamide, all chemicals purchased from Applichem). .. SDS-PAGE separation was performed using a PROTEAN plus Dodeca Cell (Bio-Rad, Munich, Germany) with gels of 1.5 mm thickness and an acrylamide/bisacrylamide matrix of 2.5% C and 13% T (Serva, Heidelberg, Germany).

Article Title: Identification of 14-3-3 β Gene as a Novel miR-152 Target Using a Proteome-based Approach
Article Snippet: Samples (500 μg of protein in a volume of 350 μl of lysis buffer) were applied to IPG strips (pH 3–10 NL, 18 cm, GE Healthcare) by in-gel rehydration and covered with 450 μl of Immobiline DryStrip Cover Fluid (GE Healthcare). .. After 2 h of rehydration, isoelectric focusing was carried out at 20 °C on an Ettan IPGphor 2 unit (GE Healthcare) at the following settings: 30 V for 10 h, 500 V for 1 h, 1000 V for 1 h, 5000 V for 1 h, and 8000 V up to a total of 45,000 V*h. The IPG strips were subjected to the strip equilibration procedure, which was performed by incubating the strips for 15 min in 12 ml of equilibration buffer (6 m urea, 2% SDS, 50 m m Tris-HCl (pH 8.8), 30% glycerol) supplemented with 1.5% DTT followed by 15 min in 12 ml of equilibration buffer supplemented with 4.8% iodoacetamide (all chemicals by AppliChem). .. SDS-PAGE separation was performed using a PROTEAN plus Dodeca Cell (Bio-Rad) with gels of 1.5 mm thickness and an acrylamide concentration of 13%.

Incubation:

Article Title: VHL-dependent alterations in the secretome of renal cell carcinoma: Association with immune cell response?
Article Snippet: After 2 h of rehydration, isoelectric focusing (IEF) was carried out at 20°C on an Ettan IPGphor 2 unit (GE Healthcare) at the following settings: 30 V 10 hrs, 500 V 1 h, 1000 V 1 h, 5000 V 1 h, and 8000 V up to a total of 45,000 Vhrs. .. The IPG strips were subjected to a 2 step strip equilibration procedure, which was performed by incubation the strips first for 15 min in 12 ml equilibration buffer I (6 M urea, 2% SDS, 50 mM Tris HCl (pH 8.8), 30% glycerol, supplemented with 1.5% DTT) followed by 15 min in 12 ml equilibration buffer II (6 M urea, 2% SDS, 50 mM Tris HCl (pH 8.8), 30% glycerol, supplemented with 4.8% iodoacetamide, all chemicals purchased from Applichem). .. SDS-PAGE separation was performed using a PROTEAN plus Dodeca Cell (Bio-Rad, Munich, Germany) with gels of 1.5 mm thickness and an acrylamide/bisacrylamide matrix of 2.5% C and 13% T (Serva, Heidelberg, Germany).



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