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basic eppendorf biospectrometer  (Eppendorf AG)


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    Structured Review

    Eppendorf AG basic eppendorf biospectrometer
    Basic Eppendorf Biospectrometer, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 202216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eppendorf+biospectrometer+basic/Eppendorf/pm41991802-57-14-15
    Average 99 stars, based on 202216 article reviews
    basic eppendorf biospectrometer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Software:

    Article Title: Gliadin-specific CD4 + T cells exhibit functional diversity independent of TCR usage or epitope specificity.
    Article Snippet: Enriched cells were then stained at 4°C for 30 min in the dark with a cocktail of fluorescently labeled surface antibodies (AF700 mouse anti- human CD14, AF700 mouse anti- human CD19, BV480 mouse anti- human CD3, and BUV395 mouse anti- human CD4 in Brilliant Stain buffer; table S12) followed by FVS700 fixable viability stain (BD Biosciences) for 10 min at RT in the dark. .. Cells were washed and the entire samples were acquired on a FACSAria III cell sorter with FACSDiva 8.0.1 software (BD Immunocytometry Systems), and live CD19−CD14− CD3+ CD4+ tetramer binding cells were singlecell index sorted into 96- well polymerase chain reaction (PCR) plates (Eppendorf #00301286) and stored at −80°C until use. .. Analysis of epitope- specific T cell repertoires For plates containing sorted DQ2.5–glia- α1a– or DQ2.5–gliaω1–specific CD4+ T cells, mRNA was reverse- transcribed in 2.5 μl using the SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific, #11754) (containing 1× VILO reaction mix, 1× SuperScript enzyme mix, and 0.1% Triton X- 100) and incubated at 25°C for 10 min, 42°C for 120 min and 85°C for 5 min.

    Binding Assay:

    Article Title: Gliadin-specific CD4 + T cells exhibit functional diversity independent of TCR usage or epitope specificity.
    Article Snippet: Enriched cells were then stained at 4°C for 30 min in the dark with a cocktail of fluorescently labeled surface antibodies (AF700 mouse anti- human CD14, AF700 mouse anti- human CD19, BV480 mouse anti- human CD3, and BUV395 mouse anti- human CD4 in Brilliant Stain buffer; table S12) followed by FVS700 fixable viability stain (BD Biosciences) for 10 min at RT in the dark. .. Cells were washed and the entire samples were acquired on a FACSAria III cell sorter with FACSDiva 8.0.1 software (BD Immunocytometry Systems), and live CD19−CD14− CD3+ CD4+ tetramer binding cells were singlecell index sorted into 96- well polymerase chain reaction (PCR) plates (Eppendorf #00301286) and stored at −80°C until use. .. Analysis of epitope- specific T cell repertoires For plates containing sorted DQ2.5–glia- α1a– or DQ2.5–gliaω1–specific CD4+ T cells, mRNA was reverse- transcribed in 2.5 μl using the SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific, #11754) (containing 1× VILO reaction mix, 1× SuperScript enzyme mix, and 0.1% Triton X- 100) and incubated at 25°C for 10 min, 42°C for 120 min and 85°C for 5 min.

    Polymerase Chain Reaction:

    Article Title: Gliadin-specific CD4 + T cells exhibit functional diversity independent of TCR usage or epitope specificity.
    Article Snippet: Enriched cells were then stained at 4°C for 30 min in the dark with a cocktail of fluorescently labeled surface antibodies (AF700 mouse anti- human CD14, AF700 mouse anti- human CD19, BV480 mouse anti- human CD3, and BUV395 mouse anti- human CD4 in Brilliant Stain buffer; table S12) followed by FVS700 fixable viability stain (BD Biosciences) for 10 min at RT in the dark. .. Cells were washed and the entire samples were acquired on a FACSAria III cell sorter with FACSDiva 8.0.1 software (BD Immunocytometry Systems), and live CD19−CD14− CD3+ CD4+ tetramer binding cells were singlecell index sorted into 96- well polymerase chain reaction (PCR) plates (Eppendorf #00301286) and stored at −80°C until use. .. Analysis of epitope- specific T cell repertoires For plates containing sorted DQ2.5–glia- α1a– or DQ2.5–gliaω1–specific CD4+ T cells, mRNA was reverse- transcribed in 2.5 μl using the SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific, #11754) (containing 1× VILO reaction mix, 1× SuperScript enzyme mix, and 0.1% Triton X- 100) and incubated at 25°C for 10 min, 42°C for 120 min and 85°C for 5 min.

    Saline:

    Article Title: Systematic identification of p11 as a signaling modulator across the GPCRome
    Article Snippet: For control purposes, untransfected Expi293F cells were grown for 48 hours in 12-well plates (Sarstedt) at the same density (3 × 10 6 /ml) as the transfected cells. .. To harvest the cells, each well was collected to a 1.5-ml Eppendorf tube, spun down at 4°C, and washed twice with ice-cold phosphate-buffered saline (PBS). ..

    Nuclear Magnetic Resonance:

    Article Title: Salivary metabolic profile in patients with oral squamous cell carcinoma and potentially malignant disorders.
    Article Snippet: .. The NMR samples were prepared by mixing 540 μl of saliva filtrate and 60 μl of potassium phosphate buffer (750 mM potassium dihydrogen phosphate, 0.1% sodium azide, 2.9 mM sodium 3-(trimethylsilyl)propionate-2,2,3,3-d4 in deuterium oxide, pH 7.0) in an Eppendorf tube, and 520 μl of the solution was transferred to a 5 mm NMR tube. .. The saliva NMR samples were measured with Bruker AVANCE III HD spectrometer (Bruker BioSpin GmbH, Karlsruhe, Germany) operating at 600.22 MHz and equipped with a cryoprobe (Bruker Prodigy TCI 600 S3 H&F-C/ND-05 Z).

    Sterility:

    Article Title: The impact of the estrous cycle on the vaginal microbiota of dairy cows
    Article Snippet: .. Fifteen circular movements were performed on the vaginal mucosa, after which the swab tip was placed into a sterile Eppendorf tube. ..

    Liquid Chromatography:

    Article Title: The Analysis of Fennel ( Foeniculum vulgare Mill.) Chemical Composition by UPLC‐Q‐Exactive‐Plus‐Orbitrap‐MS and GC‐MS/MS
    Article Snippet: .. Q Exactive Plus Orbitrap high‐resolution LC‐MS (Thermo Fisher), U3000 ultra‐high‐performance liquid chromatography system and autosampler (Thermo Fisher), ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm; Waters Corporation), 7890B‐7000D GC‐MS/MS instrument (Agilent Technologies, Santa Clara, CA, USA), HP‐5MS capillary column (30 m × 0.25 mm × 0.25 μm), Vortex 2 Genie Vortex Mixer (Scientific Industries), 5810R Low temperature centrifuge (Eppendorf), WD‐9415C ultrasonic cleaner (Beijing Liuyi Instrument Factory), AL 204 Electronic Analytical Balance (Mettler‐Toledo, USA), and KQ‐250 Ultrasonic Cleaner (Gong Yuhua Instrument Co., Ltd). ..

    Ultra-High Performance Liquid Chromatography:

    Article Title: The Analysis of Fennel ( Foeniculum vulgare Mill.) Chemical Composition by UPLC‐Q‐Exactive‐Plus‐Orbitrap‐MS and GC‐MS/MS
    Article Snippet: .. Q Exactive Plus Orbitrap high‐resolution LC‐MS (Thermo Fisher), U3000 ultra‐high‐performance liquid chromatography system and autosampler (Thermo Fisher), ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm; Waters Corporation), 7890B‐7000D GC‐MS/MS instrument (Agilent Technologies, Santa Clara, CA, USA), HP‐5MS capillary column (30 m × 0.25 mm × 0.25 μm), Vortex 2 Genie Vortex Mixer (Scientific Industries), 5810R Low temperature centrifuge (Eppendorf), WD‐9415C ultrasonic cleaner (Beijing Liuyi Instrument Factory), AL 204 Electronic Analytical Balance (Mettler‐Toledo, USA), and KQ‐250 Ultrasonic Cleaner (Gong Yuhua Instrument Co., Ltd). ..

    Gas Chromatography:

    Article Title: The Analysis of Fennel ( Foeniculum vulgare Mill.) Chemical Composition by UPLC‐Q‐Exactive‐Plus‐Orbitrap‐MS and GC‐MS/MS
    Article Snippet: .. Q Exactive Plus Orbitrap high‐resolution LC‐MS (Thermo Fisher), U3000 ultra‐high‐performance liquid chromatography system and autosampler (Thermo Fisher), ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm; Waters Corporation), 7890B‐7000D GC‐MS/MS instrument (Agilent Technologies, Santa Clara, CA, USA), HP‐5MS capillary column (30 m × 0.25 mm × 0.25 μm), Vortex 2 Genie Vortex Mixer (Scientific Industries), 5810R Low temperature centrifuge (Eppendorf), WD‐9415C ultrasonic cleaner (Beijing Liuyi Instrument Factory), AL 204 Electronic Analytical Balance (Mettler‐Toledo, USA), and KQ‐250 Ultrasonic Cleaner (Gong Yuhua Instrument Co., Ltd). ..

    Mass Measurement:

    Article Title: The Analysis of Fennel ( Foeniculum vulgare Mill.) Chemical Composition by UPLC‐Q‐Exactive‐Plus‐Orbitrap‐MS and GC‐MS/MS
    Article Snippet: .. Q Exactive Plus Orbitrap high‐resolution LC‐MS (Thermo Fisher), U3000 ultra‐high‐performance liquid chromatography system and autosampler (Thermo Fisher), ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm; Waters Corporation), 7890B‐7000D GC‐MS/MS instrument (Agilent Technologies, Santa Clara, CA, USA), HP‐5MS capillary column (30 m × 0.25 mm × 0.25 μm), Vortex 2 Genie Vortex Mixer (Scientific Industries), 5810R Low temperature centrifuge (Eppendorf), WD‐9415C ultrasonic cleaner (Beijing Liuyi Instrument Factory), AL 204 Electronic Analytical Balance (Mettler‐Toledo, USA), and KQ‐250 Ultrasonic Cleaner (Gong Yuhua Instrument Co., Ltd). ..



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