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enzyme superscript ii reverse transcriptase  (Thermo Fisher)


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    Thermo Fisher enzyme superscript ii reverse transcriptase
    Enzyme Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enzyme+superscript+ii+reverse+transcriptase/high+capacity+cdna+reverse+transcription+kit/pm38132363-101-9-14
    Average 90 stars, based on 1 article reviews
    enzyme superscript ii reverse transcriptase - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Integrative machine learning reveals the biological function and prognostic significance of α-ketoglutarate in gastric cancer
    Article Snippet: To further investigate the regulatory mechanism by which α-KG treatment suppresses GC progression, total RNA was extracted from GC cells in both treated and control groups using TRIzol ® reagent (cat. no. 15596018; Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. For transcriptomic analysis, polyadenylated mRNA was enriched using oligo(dT) magnetic beads, fragmented into short sequences and reverse transcribed into cDNA using the SuperScript III First-Strand Synthesis System (cat. no. 18080051; Invitrogen; Thermo Fisher Scientific, Inc.) with dNTPs (cat. no. R0192; Thermo Fisher Scientific, Inc.) and random hexamer primers. .. The reverse transcription temperature protocol was as follows: 2°C for 10 min, 50°C for 50 min and 70°C for 15 min. Double-stranded cDNA was synthesized, end-repaired, A-tailed and ligated to sequencing adapters using the NEBNext Ultra II DNA Library Prep Kit for Illumina (cat. no. E7645S; New England Biolabs, Inc.).

    Reverse Transcription:

    Article Title: Integrative machine learning reveals the biological function and prognostic significance of α-ketoglutarate in gastric cancer
    Article Snippet: To further investigate the regulatory mechanism by which α-KG treatment suppresses GC progression, total RNA was extracted from GC cells in both treated and control groups using TRIzol ® reagent (cat. no. 15596018; Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. For transcriptomic analysis, polyadenylated mRNA was enriched using oligo(dT) magnetic beads, fragmented into short sequences and reverse transcribed into cDNA using the SuperScript III First-Strand Synthesis System (cat. no. 18080051; Invitrogen; Thermo Fisher Scientific, Inc.) with dNTPs (cat. no. R0192; Thermo Fisher Scientific, Inc.) and random hexamer primers. .. The reverse transcription temperature protocol was as follows: 2°C for 10 min, 50°C for 50 min and 70°C for 15 min. Double-stranded cDNA was synthesized, end-repaired, A-tailed and ligated to sequencing adapters using the NEBNext Ultra II DNA Library Prep Kit for Illumina (cat. no. E7645S; New England Biolabs, Inc.).

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..

    Article Title: LncRNA levels in the central nervous system as novel potential players and biomarkers in amyotrophic lateral sclerosis
    Article Snippet: The quality and concentration of each sample were measured with Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific). .. RNA was retrotranscribed with the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific; Waltham, MA, USA). .. Quantitative PCR was performed from diluted cDNA in triplicates using the Quant StudioTM 3 Real Time PCR Instrument (Thermo Fisher Scientific).

    Random Hexamer:

    Article Title: Integrative machine learning reveals the biological function and prognostic significance of α-ketoglutarate in gastric cancer
    Article Snippet: To further investigate the regulatory mechanism by which α-KG treatment suppresses GC progression, total RNA was extracted from GC cells in both treated and control groups using TRIzol ® reagent (cat. no. 15596018; Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. For transcriptomic analysis, polyadenylated mRNA was enriched using oligo(dT) magnetic beads, fragmented into short sequences and reverse transcribed into cDNA using the SuperScript III First-Strand Synthesis System (cat. no. 18080051; Invitrogen; Thermo Fisher Scientific, Inc.) with dNTPs (cat. no. R0192; Thermo Fisher Scientific, Inc.) and random hexamer primers. .. The reverse transcription temperature protocol was as follows: 2°C for 10 min, 50°C for 50 min and 70°C for 15 min. Double-stranded cDNA was synthesized, end-repaired, A-tailed and ligated to sequencing adapters using the NEBNext Ultra II DNA Library Prep Kit for Illumina (cat. no. E7645S; New England Biolabs, Inc.).

    other:

    Article Title: Lobetyolin alleviates IMQ-induced psoriasis-like skin inflammation by maintaining the homeostasis of the skin and inhibiting the inflammatory cytokines in dendritic cells
    Article Snippet: Purified mRNA was fragmented in magnesium-containing fragmentation buffer (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.) at 94°C for 5 min. First-strand cDNA was synthesized using random hexamer-primed reverse transcription (SuperScriptTM IV Reverse Transcriptase; Thermo Fisher Scientific, Inc.).

    Article Title: Neonatal microbiota colonization primes maturation of goblet cell–mediated protection in the pre-weaning colon
    Article Snippet: Expression of Nox1 and Duox2 genes was analyzed by qRT-PCR of cDNA prepared from 600 ng RNA extractions using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific).

    Article Title: Antisense oligonucleotides targeting the SARS-CoV-2 nucleocapsid gene decrease viral titers in hamsters
    Article Snippet: Reverse transcription (RT) was performed with the SuperScript IV first-strand synthesis system (Thermo Fisher Scientific), according to the manufacturer’s directions, with 1 μg total RNA and 50 ng random hexamer primers (Thermo Fisher Scientific).

    Article Title: Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development
    Article Snippet: RT-qPCR primers ( ) were designed by the primer design program Primer3Plus or downloaded from a study by Forouhan et al. First-strand cDNA was synthetized using the High-Capacity cDNA Reverse transcription kit (Thermo Fisher Scientific) following the manufacturer’s protocol while precipitated DNA was de-crosslinked (65°C for 1 h), purified using classic phenol-chloroform, followed by ethanol precipitation.

    RNA Extraction:

    Article Title: Reduced growth and biofilm formation at high temperatures contribute to Cryptococcus deneoformans dermatotropism
    Article Snippet: .. RNA extraction from a 5 ml (1×10 7 cells/ml) yeast culture suspension was performed using a Quick-RNA fungal/bacterial extraction kit (Zymo Research), following the manufacturer's instructions; 200 ng of total RNA was used to synthesize cDNA with a Verso cDNA Synthesis kit (Thermo Fisher Scientific). ..

    Suspension:

    Article Title: Reduced growth and biofilm formation at high temperatures contribute to Cryptococcus deneoformans dermatotropism
    Article Snippet: .. RNA extraction from a 5 ml (1×10 7 cells/ml) yeast culture suspension was performed using a Quick-RNA fungal/bacterial extraction kit (Zymo Research), following the manufacturer's instructions; 200 ng of total RNA was used to synthesize cDNA with a Verso cDNA Synthesis kit (Thermo Fisher Scientific). ..

    Extraction:

    Article Title: Reduced growth and biofilm formation at high temperatures contribute to Cryptococcus deneoformans dermatotropism
    Article Snippet: .. RNA extraction from a 5 ml (1×10 7 cells/ml) yeast culture suspension was performed using a Quick-RNA fungal/bacterial extraction kit (Zymo Research), following the manufacturer's instructions; 200 ng of total RNA was used to synthesize cDNA with a Verso cDNA Synthesis kit (Thermo Fisher Scientific). ..

    cDNA Synthesis:

    Article Title: Reduced growth and biofilm formation at high temperatures contribute to Cryptococcus deneoformans dermatotropism
    Article Snippet: .. RNA extraction from a 5 ml (1×10 7 cells/ml) yeast culture suspension was performed using a Quick-RNA fungal/bacterial extraction kit (Zymo Research), following the manufacturer's instructions; 200 ng of total RNA was used to synthesize cDNA with a Verso cDNA Synthesis kit (Thermo Fisher Scientific). ..

    Amplification:

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..

    Purification:

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..

    Polymerase Chain Reaction:

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..

    Sequencing:

    Article Title: B-cell immunodeficiency associated with polynucleotide kinase 3′-phosphatase (PNKP) deficiency
    Article Snippet: Cells were irradiated with gamma rays at a 0.5 Gy/min rate using a cobalt-60 source in Chiyoda Technol Cobalt Radiation Center at the Institute of Science Tokyo. .. Total RNA was extracted from peripheral blood mononuclear cells using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich) and subjected to reverse transcription with a SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) using random primers. cDNA was amplified using VH1-6 FR1 forward primers and either the CgCH or immunoglobulin heavy chain (IGH) A reverse primer., The purified PCR products were ligated with index adapters for sequencing and analyzed on the Illumina MiSeq device using a MiSeq v3 600 cycle kit with 300 bp paired-end reads, following the manufacturer’s instructions as previously described., Data were analyzed in the immune repertoire pipeline and/or the somatic SHM and class-switch recombination pipelines from ARGalaxy (bioinf-galaxian.erasmusmc.nl/argalaxy/). ..



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