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illumina chip seq prep kit  (Illumina Inc)


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    Illumina Inc illumina chip seq prep kit
    Illumina Chip Seq Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enzymatic+chip-seq+kit/TruSeq+ChIP+Library+Preparation+Kit/pmc06028126-136-19-19
    Average 96 stars, based on 1119 article reviews
    illumina chip seq prep kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: DMRT1 is required at two steps of ovarian differentiation in rabbits.
    Article Snippet: Total RNA quality was verified on an Agilent 2100 Bioanalyzer (Matriks, Norway), and samples with an RIN > 9 were made available for RNA sequencing. .. This work benefited from the facilities and expertise of the I2BC High- throughput Sequencing Platform (www.i2bc.parissaclay.fr/sequencing/ng- sequencing/; Université Paris- Saclay, Gif- surYvette, France) for oriented library preparation (Illumina TruSeq RNA Sample Preparation Kit) and sequencing (Paired- end 50–35 bp and NextSeq500). ..

    Sequencing:

    Article Title: DMRT1 is required at two steps of ovarian differentiation in rabbits.
    Article Snippet: Total RNA quality was verified on an Agilent 2100 Bioanalyzer (Matriks, Norway), and samples with an RIN > 9 were made available for RNA sequencing. .. This work benefited from the facilities and expertise of the I2BC High- throughput Sequencing Platform (www.i2bc.parissaclay.fr/sequencing/ng- sequencing/; Université Paris- Saclay, Gif- surYvette, France) for oriented library preparation (Illumina TruSeq RNA Sample Preparation Kit) and sequencing (Paired- end 50–35 bp and NextSeq500). ..

    Article Title: Targeting TMPRSS2-ERG Fusion-Driven Prostate Cancer using Novel HBS-α-Helicomimics
    Article Snippet: Library preparation and sequencing analysis were performed by Macrogen Inc. (South Korea). .. Quality of RNA was assessed using a 2100 Bioanalyzer (Agilent Technologies), followed by library preparation (using the Illumina TruSeq Stranded mRNA Library method) and paired-end sequencing on NovaSeq with a 150 bp read length, and more than 25 M reads were recorded for each sample. ..

    Article Title: Genome-Wide Association Study of Body Weight and Body Size Traits in Wenshang Barred Chickens at the Late Rearing Stage
    Article Snippet: Genomic DNA that met the quality-control criteria was randomly fragmented to an average size of approximately 350 bp using a Covaris focused ultrasonicator (Covaris, Woburn, MA, USA). .. Sequencing libraries were constructed according to the manufacturer’s instructions using the TruSeq Library Construction Kit (Illumina, San Diego, CA, USA). .. Qualified libraries were sequenced on an Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 150-bp paired-end reads.

    Sample Prep:

    Article Title: DMRT1 is required at two steps of ovarian differentiation in rabbits.
    Article Snippet: Total RNA quality was verified on an Agilent 2100 Bioanalyzer (Matriks, Norway), and samples with an RIN > 9 were made available for RNA sequencing. .. This work benefited from the facilities and expertise of the I2BC High- throughput Sequencing Platform (www.i2bc.parissaclay.fr/sequencing/ng- sequencing/; Université Paris- Saclay, Gif- surYvette, France) for oriented library preparation (Illumina TruSeq RNA Sample Preparation Kit) and sequencing (Paired- end 50–35 bp and NextSeq500). ..

    other:

    Article Title: An epigenetic program dictates MYC amplification dynamics that promote transient, extrachromosomal, and inherited genomic events.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER McCoy’s 5A Medium Millipore Sigma Cat# M4892 Opti-Mem Life Technologies Cat# 31985070 Trypsin (0.25%) EDTA Life Technologies Cat# 2520056 L-Glutamine Life Technologies Cat# 25030-081 Penicillin and Streptomycin Life Technologies Cat# 15140122 Fetal Bovine Serum (FBS) Gibco Cat# 26140-079 Lipofectamine 3000 Life Technologies Cat# L30000015 KDM4i (QC6352) Jian Jin Chen et al.84 KDM4i (QC8222; Zavondemstat) MedChem Express Cat# HY-148807 SETD2i (EMZ0414) Jian Jin Alford et al.97 Critical commercial assays miRNeasy Mini Kit Qiagen Cat# 217004 Puregene Cell Core Kit Qiagen Cat# 1126826 Superscript IV 1st Strand System Life Technologies Cat# 18091050 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Life Technologies Cat# C10337 CL-XPosure Film Thermo Scientific Cat# 34091 Lumi-Light Western Blotting Substrate Roche Cat# 12015200001 Pierce BCA Protein Assay Thermo Scientific Cat# 23223 and 23224 Protein A Dynabeads Thermo Scientific Cat# 10002D Protein G Dynabeads Thermo Scientific Cat# 10004D TruSeq ChIP Sample Prep Kit Set A (48 samples) Illumina Cat# IP-202-1012 TruSeq ChIP Sample Prep Kit Set B (48 samples) Illumina Cat# IP-202-1024 ThruPLEX DNA-Seq Kit Takara Bio Cat# R400676 CUT&Tag-IT Express Assay Kit Active Motif Cat# 53177 CUT&Tag-IT Spike-In Control, Anti-Rabbit Active Motif Cat# 53168 NextSeq 500/550 High Output Kit v2 (75 cycles) Illumina Cat# FC-404-2005 NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit (100 Cycles) Illumina Cat# 20100987 Illumina DNA Prep with Enrichment, (S) Tagmentation Illumina Cat# 20025523 Twist Bioscience for Illumina Exome 2.5 Panel Illumina Cat# 20076914 Illumina DNA PCR-Free Prep, Tagmentation Illumina Cat# 20041795 Dead Cell Apoptosis Kits with Annexin V Life Technologies Cat# V13241 Deposited data Raw ChIP Sequencing and CUT&Tag This paper GEO: GSE315988 CUT&Tag Data This paper GEO: GSE315988 WES and WGS This paper SRA: PRJNA1393402 KYSE150 and Mouse ChIP-Seq Pedersen et al.82 GEO: GSE28332 Mouse ChIP Seq (H3K36me3) Pedersen et al.86 GEO: GSE64254 RPE siKDM5A H3K4me3 ChIP-Seq Clarke et al.63 GEO: GSE315481 Uncropped FISH Images and Blots This paper Mendeley Data: https://doi.org/ 10.17632/2wv3645ktf.1 Experimental models: Cell lines RPE-hTERT1 Nick Dyson RRID: CVCL_4388 RPE1-hTERT Cas9 TP53− /− Zimmerman et al.112 N/A HCT116 ATCC CCL-247; RRID: CVCL_0291 HL60 ATCC CCL-240; RRID: CVCL_0002 NCI-H446 Israel Canadas RRID: CVCL_1562 (Continued on next page) ll Article Molecular Cell 86, 1–21.e1–e12, December 3, 2026 e2

    Construct:

    Article Title: Geographic barrier contributed to the diversity of maternal and paternal lineages in New Guinea Island.
    Article Snippet: Library construction, sequencing and alignment were performed by Macrogen Japan (Kyoto, Japan). .. Genomic library was constructed with the TruSeq DNA PCR-Free library prep kit (Illumina) according to the Illumina TruSeq Nano DNA library preparation guide or TruSeq DNA PCRfree library preparation guide to obtain a final library of 300–400 bp average insert size. .. Sequencing was conducted on the DNA libraries using the NovaSeq 6000 System (Illumina), according to the manufacturer’s protocol.

    Article Title: Genome-Wide Association Study of Body Weight and Body Size Traits in Wenshang Barred Chickens at the Late Rearing Stage
    Article Snippet: Genomic DNA that met the quality-control criteria was randomly fragmented to an average size of approximately 350 bp using a Covaris focused ultrasonicator (Covaris, Woburn, MA, USA). .. Sequencing libraries were constructed according to the manufacturer’s instructions using the TruSeq Library Construction Kit (Illumina, San Diego, CA, USA). .. Qualified libraries were sequenced on an Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 150-bp paired-end reads.

    Polymerase Chain Reaction:

    Article Title: Geographic barrier contributed to the diversity of maternal and paternal lineages in New Guinea Island.
    Article Snippet: Library construction, sequencing and alignment were performed by Macrogen Japan (Kyoto, Japan). .. Genomic library was constructed with the TruSeq DNA PCR-Free library prep kit (Illumina) according to the Illumina TruSeq Nano DNA library preparation guide or TruSeq DNA PCRfree library preparation guide to obtain a final library of 300–400 bp average insert size. .. Sequencing was conducted on the DNA libraries using the NovaSeq 6000 System (Illumina), according to the manufacturer’s protocol.

    Article Title: Genome architecture shapes transcriptional responses to topoisomerase depletion in C. elegans.
    Article Snippet: .. For library preparation, half of the ChIP DNA and 30 ng of input DNA were ligated to Illumina TruSeq adapters and amplified by PCR. .. DNA fragments between 250 and 600 bp were gel-purified using the Qiagen gel extraction kit.

    DNA Library Preparation:

    Article Title: Geographic barrier contributed to the diversity of maternal and paternal lineages in New Guinea Island.
    Article Snippet: Library construction, sequencing and alignment were performed by Macrogen Japan (Kyoto, Japan). .. Genomic library was constructed with the TruSeq DNA PCR-Free library prep kit (Illumina) according to the Illumina TruSeq Nano DNA library preparation guide or TruSeq DNA PCRfree library preparation guide to obtain a final library of 300–400 bp average insert size. .. Sequencing was conducted on the DNA libraries using the NovaSeq 6000 System (Illumina), according to the manufacturer’s protocol.

    Chromatin Immunoprecipitation:

    Article Title: Nuclear cGAS inhibits stimulation-induced inflammatory gene transcription via HDAC1.
    Article Snippet: .. For ChIP-seq, purified ChIP DNA was used for library preparation and sequenced on an Illumina platform, followed by peak calling and genomic annotation using standar d pipelines. ..

    Article Title: Genome architecture shapes transcriptional responses to topoisomerase depletion in C. elegans.
    Article Snippet: .. For library preparation, half of the ChIP DNA and 30 ng of input DNA were ligated to Illumina TruSeq adapters and amplified by PCR. .. DNA fragments between 250 and 600 bp were gel-purified using the Qiagen gel extraction kit.

    Purification:

    Article Title: Nuclear cGAS inhibits stimulation-induced inflammatory gene transcription via HDAC1.
    Article Snippet: .. For ChIP-seq, purified ChIP DNA was used for library preparation and sequenced on an Illumina platform, followed by peak calling and genomic annotation using standar d pipelines. ..

    Amplification:

    Article Title: Genome architecture shapes transcriptional responses to topoisomerase depletion in C. elegans.
    Article Snippet: .. For library preparation, half of the ChIP DNA and 30 ng of input DNA were ligated to Illumina TruSeq adapters and amplified by PCR. .. DNA fragments between 250 and 600 bp were gel-purified using the Qiagen gel extraction kit.



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    Image Search Results


    Lactylation-driven expression of an oncogene set. a A heatmap depicts alterations in lactylation-associated gene expression and chromatin accessibility pre- and post-lactate treatment in the oral squamous cell carcinoma (OSCC) cell line CAL27. b Correlation analysis between lactylation-driven gene expression levels and chromatin accessibility. c Variations in lactylation-driven genes and chromatin accessibility in CAL27 cells exposed to high lactate concentrations. d Enrichment analysis of lactylation-driven genes and gene sets positively and negatively correlated with chromatin accessibility via single-sample gene set enrichment analysis (ssGSEA). e Intersection analysis of lactylation-driven gene sets positively correlated with chromatin accessibility and differentially expressed genes, determined by cleavage under targets and tagmentation sequencing (CUT&TAG-seq), assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), and RNA sequencing (RNA-seq). f Validation of increased lactylation enrichment in the promoter regions of CASP3 and TPR in OSCC tissues through chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR, two-tailed unpaired Student’s t -test, ** P < 0.01) and prediction of promoter sequences potentially affected by lactylation. g Validation of elevated expression of CASP3 , HSP90AA1 , HSP90B1 , TPR , RAS , and CCND1 in OSCC tissues through reverse transcription-quantitative polymerase chain reaction (RT-qPCR, two-tailed paired Student’s t -test, * P < 0.05; ** P < 0.01). Lac: lactate

    Journal: Genome Biology

    Article Title: Multi-omics reveals lactylation-driven regulatory mechanisms promoting tumor progression in oral squamous cell carcinoma

    doi: 10.1186/s13059-024-03383-8

    Figure Lengend Snippet: Lactylation-driven expression of an oncogene set. a A heatmap depicts alterations in lactylation-associated gene expression and chromatin accessibility pre- and post-lactate treatment in the oral squamous cell carcinoma (OSCC) cell line CAL27. b Correlation analysis between lactylation-driven gene expression levels and chromatin accessibility. c Variations in lactylation-driven genes and chromatin accessibility in CAL27 cells exposed to high lactate concentrations. d Enrichment analysis of lactylation-driven genes and gene sets positively and negatively correlated with chromatin accessibility via single-sample gene set enrichment analysis (ssGSEA). e Intersection analysis of lactylation-driven gene sets positively correlated with chromatin accessibility and differentially expressed genes, determined by cleavage under targets and tagmentation sequencing (CUT&TAG-seq), assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), and RNA sequencing (RNA-seq). f Validation of increased lactylation enrichment in the promoter regions of CASP3 and TPR in OSCC tissues through chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR, two-tailed unpaired Student’s t -test, ** P < 0.01) and prediction of promoter sequences potentially affected by lactylation. g Validation of elevated expression of CASP3 , HSP90AA1 , HSP90B1 , TPR , RAS , and CCND1 in OSCC tissues through reverse transcription-quantitative polymerase chain reaction (RT-qPCR, two-tailed paired Student’s t -test, * P < 0.05; ** P < 0.01). Lac: lactate

    Article Snippet: ChIP sequencing (ChIP-seq) was conducted using a ChIP-seq kit (9005S, Cell Signaling Technology) according to the provided protocol.

    Techniques: Expressing, Gene Expression, Sequencing, Next-Generation Sequencing, RNA Sequencing, Biomarker Discovery, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, ChIP-qPCR, Two Tailed Test, Reverse Transcription, Quantitative RT-PCR

    Journal: Cell Reports

    Article Title: Integrator-Dependent and Allosteric/Intrinsic Mechanisms Ensure Efficient Termination of snRNA Transcription

    doi: 10.1016/j.celrep.2020.108319

    Figure Lengend Snippet:

    Article Snippet: ChIP-seq Sample preparation kit , Cell Signaling Technologies , 9003S.

    Techniques: Recombinant, Sample Prep, Sequencing, Plasmid Preparation, Gene Expression, Control, RNA Sequencing, Western Blot, Software